Contributions of semantic and facial information to perception of nonsibilant fricatives.

Most studies have been unable to identify reliable acoustic cues for the recognition of the English nonsibilant fricatives [see text]. The present study was designed to test the extent to which the perception of these fricatives by normal-hearing adults is based on other sources of information, namely, linguistic context and visual information. In Experiment 1, target words beginning with /f/, /theta/, /s/, or [see text] were preceded by either a semantically congruous or incongruous precursor sentence. Results showed an effect of linguistic context on the perception of the distinction between /f/ and /theta/ and on the acoustically more robust distinction between /s/ and [see text]. In Experiment 2, participants identified syllables consisting of the fricatives [see text] paired with the vowels /i, a, u/. Three conditions were contrasted: Stimuli were presented with (a) both auditory and visual information, (b) auditory information alone, or (c) visual information alone. When errors in terms of voicing were ignored in all 3 conditions, results indicated that perception of these fricatives is as good with visual information alone as with both auditory and visual information combined, and better than for auditory information alone. These findings suggest that accurate perception of nonsibilant fricatives derives from a combination of acoustic, linguistic, and visual information.     

Azoospermia in two brothers with Kartagener syndrome:a family history and diagnosis

0This article describes 2 infertile brothers of asoospermia equally associated with co-existing sinusitis,bronchiectasis,and situs inversus and their family history.These two cases were diagnosed as Kartagener syndrome(KS).The patients were not offspring of kin marriages but their family members with scattered bronchi-pulmouary infections were discovered.No one manifests azoospermia,dextrocardia or situs inversus in the paternal family ex-cept two cases reported.No abnormalities were found in their mother-side family.KS should be differentiated from Young's syndrome,cystic fibrosis(CF),and other diseases such as Kallmann's syndrome in male infertility clinic.     

阴茎包埋对海绵体内一氧化氮合酶的影响

目的:探讨阴茎包埋对海绵体内一氧化氮合酶(NOS)活性的影响。方法:通过建立大鼠隐匿阴茎模型获得实验标本,分2个月组、4个月组和6个月组进行观测,每组80只大鼠。各阶段中包括包埋组(n=50)、假手术组(n=15)和正常组(n=15)。称量大鼠体重和海绵体重量后,采用化学比色法检测海绵体内NOS活性。结果:各阶段包埋组阴茎海绵体重量、体重及两者的比值与正常组和假手术组比较均无显著性差异(P>0.05);包埋降低大鼠阴茎海绵体组织的NOS活性(2个月组P>0.05,4个月组P<0.05,6个月组P<0.01)。结论:阴茎包埋可影响海绵体内NOS活性,且与包埋时间呈正相关,但对海绵体外观和重量无明显影响。     

心肌营养素1/破伤风毒素重链C端片段融合蛋白的构建及其靶向大鼠嗜铬细胞瘤细胞的研究

目的：探讨心肌营养素1（cardiotrophin 1，CT1）/破伤风毒素重链C端片段（tentanus toxin C fragment,TTC）（CT1/TTC）融合蛋白的构建及其对大鼠嗜铬细胞瘤（pheochromocytoma,PC12）细胞的靶向性。方法：采用聚合酶链式反应（PCR）、T-A克隆等分子生物学方法构建CT1/TTC融合蛋白。体外培养PC12细胞,并将CT1/TTC融合蛋白与PC12细胞共培养,红色荧光免疫组化染色后在激光共聚焦显微镜下观察融合蛋白能否在TTC的靶向作用下进入PC12细胞。结果：成功构建了CT1/TTC融合蛋白,测序显示融合基因序列正确,免疫组化染色结果显示融合蛋白能够进入PC12细胞，并发出红色荧光。结论：采用PCR和T-A克隆等分子生物学方法能成功构建CT1/TTC融合蛋白．并且TTC能够将CT1靶向进入PC12细胞。     

Fas、Bcl-2在γ射线体外诱发的白血病小鼠骨髓细胞中的表达

目的 :观察 Fas、Bcl- 2在 γ射线体外诱发的急性淋巴细胞白血病小鼠骨髓细胞中表达情况 ,以探讨辐射致癌的机制。方法 :采用 4次 1.75 Gyγ射线全身照射 BAL B/ c小鼠诱发白血病模型 ,通过流式细胞仪对照射后白血病组、未癌变组及对照组小鼠骨髓细胞中 Fas、Bcl- 2的表达进行检测 ;应用抗 Fas抗体诱导细胞凋亡 ,进一步观察 Fas、Bcl- 2的表达对骨髓细胞凋亡的影响。 结果 :白血病小鼠骨髓细胞 Fas表达较未癌变组及对照组明显下降 (P<0 .0 1) ,而 Bcl- 2的表达明显增强 (P<0 .0 1) ;白血病小鼠骨髓细胞明显耐受抗 Fas抗体诱导的细胞凋亡。 结论 :Fas低表达、Bcl- 2高表达导致了细胞凋亡受到抑制 ,这可能是辐射引起小鼠急性淋巴细胞白血病的机制之一。     

以新生儿重症监护病房为中心的早产儿视网膜病变筛查模式

目的：介绍一种较为完善的早产儿视网膜病变（ROP）筛查模式。方法：建立基本完善的ROP登记、会诊、筛查、转诊等制度,所有工作均在新生儿重症监护病房（N ICU）完成,形成以NICU为中心的ROP筛查模式,并按此模式开展筛查工作。结果：登记符合ROP筛查标准的患儿239例,其中接受至少一次筛查的患儿222例（占应筛查总数的92.89%）,按要求完成筛查的患儿176例（占应筛查总数的73.64%）。发现ROP患儿39例（占实际筛查数的17.57%）,其中阈值前期1型或阈值期病变11例（占实际筛查数的4.96%）。结论：这种以NICU为中心的ROP筛查模式,制度较为完善,临床中易于操作,能有效节约医疗资源,适合在中小城市中推广。     

结核分枝杆菌Rv1009基因蛋白的克隆和表达

目的 克隆表达结核分枝杆菌促Rv1009基因,序列测定正确后进行融合、表达.方法 采用热启动聚合酶链反应（Polymerase Chain Reaction,PCR）从结核分枝杆菌H37Rv基因组中扩增出Rv1009编码基因,用限制性内切酶消化后插入pGEX 4T-2载体中,将重组质粒转化大肠杆菌BL21（DE3）,目的基因经IPTG诱导,表达Rv1009基因蛋白.结果 经PCR扩增在1300bp处发现一条目的片段,获得了结核分枝杆菌H37Rv株Rv1009基因蛋白,经诱导后高效表达分子量为64KD的外源蛋白,与预期分子量大小一致,凝胶自动扫描分析,在A600值为0.6,IPTG终浓度为0.3 mmol/L,诱导表达3 h时融合蛋白表达量即达峰值,占菌体总蛋白的22.8%.结论 构建了结核分枝杆菌Rv1009基因重组表达载体,获得了RPF样融合蛋白的高效表达,为今后深入研究奠定了基础.     

免疫抑制剂SIIA9268A的研究.Ⅰ.发酵、分离及理化性质

在用含ｒａｐａｍｙｃｉｎ结合蛋白基因（ＲＢＰ）的啤酒酵母ＲＳ１８８Ｎ及其变株ＲＳ１８８Ｎ△ｒｂｐ：：：ＬＥＵ筛选免疫抑制剂的过程中，得到活性菌株Ｎｏ．ＳＩＩＡ９２６８（鉴定为吸水链霉菌东湖变种）。应用硅胶柱层析和制备ＴＬＣ等方法从其发酵液中分离出活性化合物ＳＩＡ９２６８Ａ。经理化性质鉴定与ｒａｐａｍｙｃｉｎ同质。     

加强思想政治工作 促进医院后勤管理

我院现有后勤职工72名，占全院总人数的7％，其中党员12名。党员占后勤职工16％。笔者自担任后勤分管领导以来，一直重视狠抓后勤党员队伍建设，充分发挥其先锋模范作用，增强带头示范效应。提升后勤队伍凝聚力，使得后勤队伍的工作积极性得到较大提高，后勤部门的服务效率、保障功能和职工满意度都取得新的突破。[第一段]