颈椎肿瘤单侧关节突关节切除后的稳定性重建

目的：探讨颈椎肿瘤单侧关节突关节切除后稳定性重建的方法及效果。方法：对1999—2005年存我院骨科手术治疗且得到随访的18例切除单侧关节突关节的颈椎肿瘤患者的资料进行分析，男10例，女8例；年龄16～72岁，平均46岁。神经根受压表现为主者10例，VAS评分2～8分，平均4．2分；脊髓压迫表现为主者8例．ASIA分级C级5例．D级3例。均采用颈后路患侧关节突关节、侧块切除，完整切除肿瘤组织，其中10例行单侧侧块钢板固定植骨融合，8例行双侧侧块钢板固定植骨融合。结果：随访3—60个月，平均20个月，1例透明细胞癌肺转移患者死亡．余存活无复发。10例神经根受压表现为主者术后疼痛VAS评分0—4分，平均1．6分。8例脊髓压迫表现为主者，5例术前C级者术后C级2例、E级3例，3例术前D级者术后D级2例．E级l例。双侧侧块钢板固定植骨融合者术后3个月4例m现骨性融合（其中1例3个月后失访），6个月7例达到骨性融合，内固定无断裂、松动、移位。无颈椎不稳。单侧侧块钢板固定植骨融合者．1例术后5个月出现颈部疼痛；9例在术后9个月骨性融合；1例12个月时仍未能骨性融合，螺钉松动。结论：颈后路侧块钢板同定植骨融合可以实现颈椎肿瘤单侧关节突关节切除后的颈椎稳定性重建。     

Body mass index, percent body fat, and bone mass in a cohort of Chinese twins aged 6 to 18 years

Introduction This report examines the relationship of body mass index (BMI), percent body fat (%BF), and bone mass in a cohort of male and female twins recruited from Anhui province, China, ages 6–18 years—577 male pairs (mean age = 11.4) and 478 female pairs (mean age = 11.6). Methods Whole body bone mineral content (WBMC) in (g), whole body bone area (WBA) in (cm²), and %BF were measured using DEXA (Lunar Prodigy, USA). Regression analysis of within-pair differences was used to assess the strength of the association, and the analysis was stratified by gender and age group, where age cut-offs were based on ages at spermarche or menarche estimated from large population based studies in China. Males were stratified at ages before 14 and age 14–18, and females at ages prior to 12 and age 12–18. Results Univariately, BMI and %BF were associated with WBMC and WBA in the younger males and females, and in older males; %BF was significant only in older females. Multivariate models included both BMI and %BF. Among the younger males, age < 14, BMI and %BF were significantly associated with WBMC and WBA. In the younger females, age < 12, %BF was only significant to WBA. In the older age group, only BMI was significant to WBMC and WBA in females, but in males, BMI was positively associated, and %BF was negatively associated with both bone measures. Discussion These findings show that association between BMI and %BF and bone mass differ across gender and developmental stages, and %BF appears to be beneficial at younger ages, but detrimental or non-beneficial at older ages of development.     

Exogenous advanced glycosylation end products induce diabetes-like vascular dysfunction in normal rats: a factor in diabetic retinopathy

BACKGROUND. Diabetic retinopathy has been shown to be directly associated with the degree and duration of hyperglycemia, and advanced glycosylation end products (AGEs) have been implicated in this pathological process. The purpose of the experiments reported here was to study the effect of AGE deposition on retinal vascular damage which leads to diabetic retinopathy. METHODS. Intravenous injection of exogenous AGEs was used to treat wild-type non-diabetic Sprague-Dawley rats. One of the two retinal slides from each animal was treated using immunohistochemical staining to label retinal vascular AGE deposition, the other H&E staining for counting of capillary pericytes. The results were compared with the findings in untreated wild-type and diabetic controls and in rats treated with unmodified rat serum albumin (RSA). RESULTS. After 2 weeks of continuous treatment, AGEs were identified in the retinal vascular tissue of the AGE-RSA-injected group. The average number of retinal capillary pericytes per 10x100 microscope power field was 4.313+/-0.34 (mean +/- SD) in the AGE-RSA-injected group, compared with 5.798+/-0.481 in the control group ( P<0.01). CONCLUSION. These experiments demonstrate that AGEs, independent of other metabolic factors, can induce vascular change resembling that of diabetic retinopathy.     

耐磷酸盐土霉素菌株选育和发酵的研究

以土霉素生产菌株138^#为出发菌株，经饥饿培养、5-FU、UV、咖啡因复合诱变处理，再用含高磷酸盐的培养基定向筛选，得到高产突变菌株100^#。该突变株遗传特性稳定，代谢特性优于出发菌株，经57m^3发酵罐生产验证，生产稳定，低效价罐批减少59．5％，在磷酸盐浓度提高到0.04％～0.05％时，统计一年的正常罐批平均发酵效价比对照菌株提高5．76％。     

浙江医科大学附二院门诊处方中精神药物使用调查

本文对浙江医科大学附属二院１９９３年７月间３１３７８张合格处方进行统计分析，并用限定日剂量（ＤＤＤ）和药物使用指数（ＤＵＩ）为指标，分析镇静催眠药物使用情况。结果表明苯二氮　类药方２０６７张，占总处方量的６．５９％，巴比妥类药方４９张，占０．１６％，麻醉药方１１１张；占０．３５％。苯二氮　类药方中．女性处方量１２０２张，男性处方量８６５张，男、女组间比较有显著性差异。苯二氮　类药方多分布在神经内科，常用的药有舒乐安定和阿普唑仑。九种常用的苯二氮　类药和麻醉性镇痛药的ＤＵＩ均小于１。提示药物滥用现象少见，该院对这类药物的管理比较严格。麻醉性镇痛药中二氢埃托啡处方占６７．５７％。     

吗啡的呼吸抑制作用与中枢胆碱能系统的关系

本文以兔呼吸频率、通气量和血气作为指标,观察到iv吗啡0.5～4.0mg/kg产生剂量依赖性呼吸抑制。icv匹鲁卡品2.5mg/kg能完全逆转这一抑制效应。icv 4—氨基吡啶(4—AP),1.5μg/kg兴奋呼吸,并使吗啡量效曲线右移。用利血平耗竭儿茶酚胺后,吗啡仍能抑制呼吸,4—AP可消除之。表明吗啡抑制呼吸与中枢胆碱能系统有关,且可能系它抑制Ach释放所致。     

Effect of eucalyptus globulus oil on activation of nuclear factor-kappaB in THP-1 cells]

OBJECTIVE: To study the effect of eucalyptus globulus oil on the activity of nuclear factor-kappaB(NF-kappaB) in THP-1 cell line. METHODS: THP-1 cells were cultured with or without eucalyptus globulus oil at different concentrations (1, 10, 100 mg x L(-1), 30 min) before being stimulated with lipopolysaccharide (LPS, 1 mg x L(-1), 30 min). The location of NF-kappaB p65 subunit (NF-kappaB/p65) in THP-1 cells was detected by indirect immunofluorescence and laser scanning confocal microscope. The expression of NF-kappaB/p65 in nuclei was measured by Western-blot analysis. RESULT: The FITC-label NF-kappaB/p65 was mainly located in the nuclei after THP-1 cells were stimulated with LPS. Whereas, no fluorescence were seen in the nuclei of cells pretreated with eucalyptus globulus oil. This effect on NF-kappaB/p65 nuclear translocation was in a concentration dependent manner. CONCLUSION: Eucalyptus globulus oil inhibits the nuclear translocation of NF-kappaB induced by LPS in THP-1 cells.     

酸性环境介导皮质神经元损伤及阻断酸敏感离子通道1α的神经保护作用

目的 探讨脑梗死模型中酸性环境能否介导皮质神经元损伤及阻断酸敏感离子通道1α（acidsensing ion channels1α，ASIC1α）的神经保护作用。方法 制作缺氧细胞模型、脑梗死动物模型，检测ASIC1α阻断剂对乳酸脱氢酸（lactate dehydrogenase，LDH）释放率、大鼠行为学、脑梗死体积以及ASIC1蛋白表达的影响。结果 酸性环境可以损伤皮质神经元，加重缺氧对细胞的毒性作用，经侧脑室注射ASIC1α阻断剂可以改善脑梗死大鼠的行为学改变，梗死体积明显减小（P〈0.05）；随梗死时间延长，缺血半暗带ASIC1表达增加，在缺血后24h表达量较明显。结论 酸性环境导致皮质神经元损伤，其损伤机制与缺血后神经元ASIC1α开放以及表达增加有关；阻断ASIC1α具有神经保护作用。     

非体外循环冠状动脉搭桥术临床分析（附11例报告）

（1）目的 总结探讨11例非体外循环冠状动脉搭桥术（OPCAB）的经验。（2）方法 2001年6－12月对11例冠心患采用非体外循环心脏不停跳方法完成冠状动脉搭桥术。（3）结果 共搭桥35支，平均3．17支／例，其中左乳内动脉桥8支，大隐静脉桥27支。6例患（54．5％）未血，无手术死亡及并发症。（4）结论 非体外循环搭桥术克服了体外循环搭桥术（CABG）的诸多缺点和弊端，手术简化，时间缩短，适应证扩大，并发症明显减少，效果满意。     

TD清洗消毒剂对口腔科器械消毒效果探讨

通过实验室和口腔科临床观察表明:TD清洗消毒剂(氯化磷酸三钠)对金黄色葡萄球菌、绿脓杆菌和枯草杆菌黑色变种的平均杀菌率为100.0％,对口腔科器械消毒无菌率为100％,对被HBsAg阳性血清污染的器械浸泡30分钟转阴率为98.1％。其消毒效果优于新洁尔灭,与戊二醛相同。在口腔科使用两年,未见金属器械有锈斑生成。