替代神经吻合法治疗喉返神经麻痹的解剖学研究

通过对30例人喉神经、膈神经、颈袢的解剖观测及利用图象分析仪二维重建技术对相关神经的截面积、纤维数量的测量比较分析,结合有关实验研究资料,设计了在甲状软膏板后下方开窗,于甲杓肌神经起点处切断该神经,用颈袢主支与甲杓肌神经吻合,用膈神经与喉下神经吻合,同时重建声带的内收和外展功能,并探讨了其解剖和生理学基础,认为此手术方法治疗喉返神经麻痹比较理想和可行。     

Advanced Chinese NiTi alloy wire and clinical observations.

Chinese NiTi wire was studied on the bench with six other nickel-titanium-alloy wires. Bending and torsional tests were conducted and temperatures of phase transformation compared. The Chinese NiTi wire was found to have a low stiffness, high springback and constant bending and torsional moments on unloading, in a very large deformation region. It can produce a gentle, nearly constant force. These factors make it desirable for clinical application. Included in this paper are clinical observations of case selected from over 100 patients in current treatment with Chinese NiTi wires. Chinese NiTi wire reduced the leveling and alignment phase of treatment without discomfort to the patient. Chinese NiTi wire can be used in both children and adults.     

ATP片剂的UV—HPLC测定

采用ＵＶ－ＨＰＬＣ法测定ＡＴＰ片剂的含量，较地方标准收载的方法方便易行，重现性好。测定的回收率为１００．８±１．５％。     

Pharmacokinetics of Controlled Release and Immediate Release Morphine Sulphate Tablets after a Single Dose and Multiple Doses in Chinese Volunteers

健康志愿者１０名，随机交叉口服硫酸吗啡控释片（ＣＲＭＳ）３０ｍｇ（３０ｍｇ×１）和硫酸吗啡普通片（ＩＲＭＳ）２０ｍｇ（１０ｍｇ×２），分别于服药前后各时点取静脉血，用ＧＣＭＳ测定血浆中吗啡含量。以药代软件程序处理，分别求得ＣＲＭＳ和ＩＲＭＳ的Ｃｍａｘ为１９．３８±３．８０和２１．２７±６．２１ｎｇ／ｍｌ；ｔｍａｘ为２．３６±０．３７ｈ和０．５５±０．１６ｈ；ｔ１／２β为３．５３±０．８７ｈ和３．０３±０．７４ｈ，曲线下面积ＡＵＣ为１４５．１５±１７．６５和９３．０８±１６．６５ｎｇ·ｈ／ｍｌ。癌症病人多次口服硫酸吗啡至稳态，ＣＲＭＳ和ＩＲＭＳ的峰浓度分别为２７．４３±０．３３ｎｇ／ｍｌ，２２．６８±０．１６ｎｇ／ｍｌ；谷浓度分别为１９．４５±１．４４ｎｇ／ｍｌ；１８．１４±０．４９ｎｇ／ｍｌ。     

Proliferative activity of calcifying odontogenic cysts as evaluated by proliferating cell nuclear antigen labeling index

The calcifying odontogenic cyst (COC) presents with diverse hlstologlcal features; thus, several subclasslfl-cations have been proposed. To evaluate the slgnlficance of the various histological features and subtypes of COC from the perspectlve of proliferative activity, the proliferating cell nuclear antigen (PCNA) labellng index (LI; the percentage of positive nuclei) was assessed immunohistochemlcally in 25 cases of COC (21 benign and four malignant). All of the benign cases were of the cystic variety and further subclas-sified into non-proliferative subtype (NPS; four cases); proliferative subtype (PS; eight cases); and COC associated with odontoma (COCaO, nlne cases). The PCNA U of the mallgnant COC (65.2 ± 5.6) was slgnlflcantly higher than that of the benlgn COC (11.6 ± 9.0; P = 0.002). Non-proliferative subtype (6.8 ± 2.8) showed the lowest PCNA LI and PS (17.2 ± 11.2) the highest of among the three subtypes of benign cystic COC (P = 0.028). In nine cases of COCaO, six showed epithelial lining of the non-proliferative type as NPS and the other three had lining wlth proliferative features as PS. The PCNA LI of the latter COCaO group (14.3 ± 6.6) was significantly higher than that of the former (6.1 ± 4.3; P = 0.05), as Seen between PS and NPS. These results demonstrate that PCNA LI is a possible parameter for differentiating mallgnant COC from benign COC and, whatever the subtypes, the proliferative features In the lining are the main factor influencing the prollferatlng actlvity of COC.     

中央空调冷却塔水中嗜肺军团菌的调查

目的：了解医院、宾馆空调冷却塔水中军团菌污染情况。方法：于2001年8月采集W市2家宾馆和4家医院的中央空调冷却塔水样，过滤空调冷却水的滤膜经盐酸处理后其处理液接种于GVPC平板，烛缸培养后选取可疑菌落进行生化试验、血清学试验并以PCR方法鉴定。结果：W市2家医院及1家星级宾馆的中央空调冷却塔水中分离到5株军团菌，2株为嗜肺军团菌血清1型（Lp1），2株为嗜肺军团菌血清5型（Lp5）。结论：应加强对医院、宾馆空调冷却塔水中军团菌的监测。     

带真皮下血管网超薄皮瓣血运重建的实验研究（三）超薄皮瓣的再血管化与血供变化

以猪作为动物模型，对带真皮下血管网超薄皮瓣的血运重建规律进行了探讨。以超薄皮瓣为实验组，保留真皮下血管网皮片为对照组，按自身配对原则随机分为６个组。通过荧光素显色，透明标本，组织学，明胶墨汁灌注过程中超薄皮瓣的染色情况等观察，发现超薄皮瓣的再血管化发生在术后第５天，至第７天新生血管通过塑形趋于成熟。它既发生在皮瓣与受床之间，又发生在皮瓣与创缘之间，而且由皮瓣成活部分远端渐至近端。     

果胶酶治疗胃石症的临床研究

目的 探讨胃石症的发病机制。分析和总结果胶酶治疗胃石症的疗效。方法 在体外将山楂，柿子嚼碎后置入新鲜胃液，分为加白酒和不加白酒两组后放入37℃恒温箱内，观察胃石形成的经过及时间，再分别加入果胶酶，观察其解聚过程及时间，对54例胃石症患服用果胶酶治疗并观察疗效。结果 加白酒组形成胃石的速度较快，果胶酶能有效溶解胃石。54例患服用果胶酶6h后吞钡透视见胃石完全消失，症状，体征完全缓解。结论 果胶酶能有效溶解胃石。是治疗胃石症的有效方法。     

人外周血单核细胞和脐血CD34+细胞来源的树突细胞比较

目的比较从人外周血单核细胞及脐血CD34+细胞诱导树突细胞(DC)的方法及其特性.方法用淋巴细胞分离液分离外周血单个核细胞,贴壁法获得单核细胞;经GM-CSF及IL-4诱导获得DC.磁性活化细胞分选系统(MACS)分选脐血CD34+细胞,以GM-CSF、IL-4、TNF-α、SCF、FL、TPO等细胞因子诱导获得DC.用电镜、普通显微镜观察DC形态;流式细胞仪分析DC表型;同种混合淋巴细胞反应(allo-MLR)观察DC刺激T淋巴细胞增殖的能力.结果外周血单核细胞在因子GM-CSF、IL-4、TNF-α,脐血CD34+细胞在GM-CSF、IL-4、TNF-α、SCF、FL、TPO等因子作用下,可生成DC,并表达相应的DC分化抗原CD14、CD80、CD83、CD86、HLA-DR.脐血CD34+细胞具有较强的扩增能力,经2周诱导,体系细胞数可增加173.8士26.7倍,两种来源的DC均能刺激同种异体淋巴细胞的增殖反应.结论外周血单核细胞与脐血CD34+细胞,在体外均可诱导成DC,并具有相应的DC分化抗原和功能.     

第四脑室囊虫病诊断及手术治疗

目的：探讨四室脑囊虫诊断及治疗方治。方法：回顾分析1992年5月--2002年12月收治的四室脑囊虫21例，术前所有患者行头颅CT扫描，其中15例MR检查，21例患者行血清及脑脊液囊虫ELISA检查。所有患者术前1d行脑室外引流，均经枕下正中入路行四室囊虫摘除术，其中1例行脑室腹腔分流术。结果：CT扫描示四脑室呈囊性扩张且伴脑积水，MRI矢状位T1加权像示四室完整的包囊。血清囊虫ELISA检查19例阳性，脑脊液21例阳性。所有患者术后高颅压症状均立即解除，随访27．4月未见复发。结论：MRI是诊断四室脑囊虫最准确的方法。血清囊虫-ELISA检查简便，但脑脊液检查比血清更敏感。术前脑室外引流很有必要，枕下正中入路四室囊虫摘除术是有效的治疗方法。