Evaluation of different methods to determine the loading of proteins in PLGA microspheres

Various determination methods for protein encapsulation efficiency of PLGA microspheres were compared. Acetonitrile is recommended as an extraction solvent.     

Potential diagnostic value of pancreatic isoamylases for pancreaticobiliary maljunction with mild biliary dilatation in patients and a porcine model

Purpose

The aim of this study was to evaluate the diagnostic value of serum pancreatic isoamylases for pancreaticobiliary maljunction (PBM) with mild biliary dilatation.

Methods

Serum and bile from 8 children with PBM and mild biliary dilatation (6 to 11 mm in diameter) and 4 young pigs with an anastomosis constructed between an isolated pancreas-duodenal segment and the gallbladder were studied for pancreatic isoamylases. Using an electrophoretic technique, the assay of pancreatic isoamylases was expressed by peak appearance rate (PAR). Serum from 20 healthy children served as normal controls.

Results

In the serum of the patients, preoperatively there were 5 pancreatic peaks with PAR as follows: P1, 100%; P2, 100%; P3, 100%; P4, 100%; and P5, 66.7%. These abnormal pancreatic isoamylases disappeared 2 weeks after operative treatment. In normal controls, there were only P1 (PAR, 40%) and P2 (PAR, 100%). Mild cylindrical dilatation (6 to 8 mm in diameter) of the common bile duct developed in the porcine PBM model. There were P1 (PAR, 100%) and P2 (PAR, 100%) in the porcine serum preoperatively. Thirty days and 60 days after establishing the model, there appeared in the serum 6 pancreatic peaks with PAR as follows: P1, 100%; P2, 100%; P3, 75%; P4, 100%; P5, 100%, and P6; 75%. The bile patterns of pancreatic isoenzymes in the patients and pigs were similar to those in serum.

Conclusions

Abnormal pancreatic isoamylases are characteristically present in the serum from both children and a porcine model of PBM and mild biliary dilatation. Assay for these abnormalities is promising to recognize this subset of patients in whom diagnosis remains a challenge.     

钛瓷修复体的颜色稳定性

目的探讨钛瓷修复体在制作过程中经多次烧结处理后表面颜色的改变规律.方法设计制作钛瓷修复体标准试件,经过l、2、4、7、10次烧结处理,每次烧结后做色度测定.结果除a*和Hab外,其他色度指标差异均无显著性.a*和Hab值的差异主要表现在烧结l、2次与烧结7、10次之间,各组间的色差值均小于1.0.结论在正常的修复体制作过程中,烧结次数在7次以内,钛瓷修复体表面颜色基本无改变.     

微粉化乌贝分散片工艺研究及药效验证

目的筛选微粉化乌贝分散片的处方和制备工艺,对剂型改变前后的药效进行比较。方法按照《中国药典》2005年版附录对分散片的要求,以休止角、硬度、外观、脆碎度、分散均匀性做为考察指标筛选处方和工艺,采用小鼠利血平胃溃疡模型考察两种剂型药效变化。结果按优选处方制备微粉化乌贝分散片,可在3min内完全崩解,且同等剂量下药效优于散剂。结论微粉化乌贝分散片服用、携带及药效明显优于散剂。     

人工麝香缓释片的制备工艺研究

目的探讨人工麝香缓释片的制备工艺。方法以人工麝香为模型药物,乙基纤维素为主要骨架缓释材料,采用传统溶剂法和改良溶剂法制备固体分散体后压片,测定其体外释放度。结果X-射线实验表明药物以非晶态存在,体外释放度结果表明,改良溶剂法优于传统溶剂法。结论用改良溶剂法制备人工麝香缓释片。     

氟西汀与马来酸曲美布汀联合治疗功能性消化不良的临床观察

目的:探讨氟西汀与马来酸曲美布汀联合治疗功能性消化不良(FD)的疗效。方法:将门诊160例FD患者随机分成甲、乙两组,甲组80例予以马来酸曲美布汀治疗,乙组80例予以马来酸曲美汀与氟西汀联合治疗,治疗时间均为8周,以自制FD症状评定量表评定疗效。结果:甲、乙两组有效率分别为62.5%(50例),91.25%(73例),疗效差异有显著性(P<0.01)。结论:氟西汀与马来酸曲美布汀联合使用可显著提高治疗FD的疗效,值得推广。     

Solid Pseudopapilllary Tumor of the Pancreas： a Case Report

Solid pseudopapillary tumors （SPT） to be a rare low-grade malignancy of the pancreas are considered that mainly appears in young women. It accounts for less than 1% of all pancreatic neoplasms and is pathologically distinctive from other types of pancreatic cancers. Curative resection is the optimal choice for SPT and the 5-year survival rate of SPT is about 95%. Here we report a case of a young girl who presented with this rare pancreatic tumor.     

防范医疗缺陷提高中医医院医疗质量

中医医院医疗缺陷的发生与大型综合性医院相比,既有普遍性,也存在着特殊性。笔者结合工作实际,分析三年中受理的39件医疗事件存在的缺陷环节,提出在中医医院中强化执行中医诊疗常规、重视诊断意识、会诊意识、建立完善的医政管理制度以及提高各级各类人员业务水平等措施。