女孩雌激素受体基因6号内含子区域两个新SNPs位点报道

目的：在女性性早熟患儿中检测雌激素受体基因6号外显子及内含子基因序列可能存在的突变。方法：PCR扩增雌激素受体基因6号外显子及内含子，对扩增产物进行DNA序列分析。结果：在6号内含子一侧，发现两个100％连锁的单核苷酸多态性(SNPs)，分别为．53G—67A和53T—67G。结论：这一对单核苷酸多态性并不引起氨基酸的替代，与女孩性早熟的发生也无关，但是否和其他疾病有连锁关系尚待进一步探讨。     

ACTIVIT OF HUMAN PLACENTAL GAMMA GLOBULININ BLOCKING IMMUNE FUNCTIONS IN VITRO AND IN ABROGATING THE XENOGENEIC, LOCAL GRAFT-VERSUS-HOST-REEACTEON

In order to understand the mechanism of immunosuppression caused by infusion of placental gamma globulin (PGG) in patients with renal allografts, rheumatoid arthritis, and graft – versus –host disease (GCHD) following bone marrow transplantation (BMT) ,we have examined the effect of PGG in vitro and in a model of the xenogeneic , local graft –ver- sus – host reaction (LGVHR) .PGG inhibited lymphocyte proliferation in mixed lymphocyte cultures (MLC) (P<0.005) and depressed interleukin -2 (IL-2) levels in such cultures at 72 hours (P<0.01) . In contrast phytohemagglutinin (PHA) –and pokeweed mitogen (PWM) –induced T and B lymphocyte blastogenesis was not affected by such PGG treatment .PGG treatment .PGG neither decreased the [3H] TdR pulse incorporation in unstimulated lymphocytes nor affected cell viability .Cell cycle analysis by flow cytometry showed that PGG reduced the percentage of cells in S and G2, M phases during the MLC, but did not alter cell cycling during PWM-stimulated proliferation . An immunosuppressive effect of PGG on the LGCHR was tested in a model of intracutaneous transplantation of PGG –treat human lymphocytes into cyclophosphamide – immunosuppressed rats. Lymphoprep – separated human tonsillar lymphocytes were incubated with RPMI-1640 buffer containing：（1）PGG,4mg/ml,(2) human plasma albumin,4mg/ml,(3)mitomycin-C,25ug/ml, or (4) no additive. Cell of each preparation (3x107cells in 0.1ml) were injected intracutaneously into cyclophosphamide-treated male rats at separate abdominal locations. A fifth site received only the buffer solution. Five days after injection of cells ,each rat received [125 I]IUdR (10uCi) intraperitoneally and was killed after 5 hours. For each site of injection, the diameters of induration were measured and 125 I was counted . There was no difference between buffer – treated and a ibumin – treated groups either in the diameter of the area of induration (t=0.66;P>0.5)or in radioactive counts(t=0.22;P>0.05).In the PGG –treated group, the induration and radioactivity measurements were significantly less than in control groups (t=3.72 and P<0.1;t=2.62 and P<0.02,respectively ) Cytophilic antibodies in PGG were thought to inhibit an early phase of T cell activation, and not to be cytotoxicity .In the LGVHR, the immune response might be abrogated either by immuno- regulatory suppression of T cell function or by toxicity to the infused lymphoid cells. For some clinical purposes, immuno- modulating, human antibodies might be preferred to murine, monoclonal antibodies.     

湿润暴露疗法治疗会阴部Ⅱ度烧伤患者32例临床分析

目的：探讨湿润暴露疗法治疗会阴部Ⅱ度烧伤患者的临床效果。方法：将我院2005年6月～2006年6月间规范应用湿性医疗技术治疗的32例会阴部烧伤患者进行临床分析、总结。结果：全部病例均治愈,且烧伤创面愈合快,不形成瘢痕。结论：应用MEBT／MEBO治疗会阴部烧伤,方法简单,护理方便,临床疗效满意,值得推广。     

神经导航内窥镜辅助下单鼻孔入路垂体腺瘤切除

目的神经导航、内窥镜辅助下垂体腺瘤的经单鼻孔经蝶手术治疗。方法对6例经磁共振成像诊断垂体腺瘤的病人术前进行磁共振成像定位,将影像资料输入导航工作站,作术前手术计划。采用零度及45度镜经单鼻腔,在导航的动态指引下确认鞍底,于内窥镜下实施瘤实质切除。结果本组5例完全切除,1例因为瘤实质较韧而作大部分切除,术后病人的视力均得到了改善。导航预期误差1.48mm,实际误差1.8mm。结论神经导航引导下的经鼻蝶垂体腺瘤手术定位准确,内窥镜下操作对鼻腔的解剖结构影响小、组织损伤轻,病人恢复快。     

手助腹腔镜肝部分切除术治疗原发性肝癌

目的 探讨手助腹腔镜肝部分切除术治疗原发性肝癌的方法和应用价值。方法 经临床筛选的第Ⅱ、Ⅲ、Ⅵ段或边缘型的原发性肝癌6例，在腹腔镜辅助下．应用手助器、超声刀、切割器等特殊器械进行腹腔镜下肝癌切除术。结果 6例患者均成功完成手术，包括肝Ⅱ、Ⅲ段切除，Ⅵ段切除及非规则性肝段切除术，手术时间80～130min，术后住院6～9d，手术出血150～700ml，无中转开腹手术，无并发症。术后随访6～15个月，2例分别于手术后3和4个月肝内复发，其余4例随访至今无复发。结论 在经过临床选择的病例中，手助腹腔镜下作肝部分切除术治疗原发性肝癌是安全、可行的手术方式。     

复发性脑胶质瘤及其放射治疗

1976～1986年肿瘤科收治脑胶质瘤42例,10例复发,其中6例采用单纯放疗,用~60钴γ线。5例脑胶质瘤予肿瘤区局部照射,DT44～57Gy/6-7周,1例髓母细胞瘤予全脑、肿瘤区及全脊髓照射、脑中平面量DT38Gy/5周;肿瘤区51Gy/7周;脊髓18Gy/8周。结果:有效1例,显效2例,无效3例。本研究提示对于已不能釆用其他手段治疗的复发病人,放疗仍有一定效果。     

Distribution of rat facial motoneurons and its reorganization following nerve reinnervation]

OBJECTIVE: To examine the distribution of rat facial motoneurons contributing different branches under normal situation and when nerve reinnervation occurred following facial nerve axotomy. METHODS: The normal distribution of motoneurons innervated both buccal and marginal mandibular branches and its reorganization after facial nerve reinnervation was observed using retrograde labeling with fluorescein. RESULTS: Under normal situation, the motoneurons contributing buccall and marginal mandibular branches were primarily distributed in the intermedial and lateral subnucleus in facial nucleus and almost completely overlapped. The two types labeled neurons organized closely, but there were no double-labeled neurons. Although the motoneurons contributing buccall and marginal mandibular branches were primarily overlapped 4 month post-anastomosis, the number of the labeled neurons obviously decreased and the organization got more scattered. There were 10% of buccall branches, 5% of marginal mandibular motoneurons in the dorsal subnuleus, 1% of buccall and 4% of marginal mandibular in dorsal ventral and medial subnucleus. The distribution pattern of the motoneurons 6 month post-anastomosis was similar to that of 4 month post-anastomosis, but the number of the labeled neurons increased, and there were 1%-2% double-labeled neurons. CONCLUSION: The distribution pattern of motoneurons innervated both buccal and marginal mandibular branches indicates that it should exist wide-spread communicating branches, and its reorganization after facial nerve reinnervation suggests that misdirected regeneration occurs among motoneurons innervating different branches.     

静脉输液加药后的微粒变化

本文对我院急诊病人常用的供静脉给药的22种药物、42组配伍情况,制成静脉加药输液,在超净工作台条件下,用KF—4型微粒计数器,测定了168次微粒数,结果显示输液加药后微粒虽有增加,但均在药典规定范围内。输液中加1种、2种、3种药物后所产生的微粒数分别为x_1=5.52(n_1=16);x_2=8.17(n_2=18)、x_3=10.25(n_3=8),经统计处理,三组间无显著差异(P<0.05)。本文对产生微粒的药物、注射器、操作环境等因素进行了分析。     

Experimental study on the immunosuppressive effects of gui zhi tang

In this paper the immunosuppressive effects of Gui Zhi Tang (a famous Chinese medicine) on the murine immune functions are reported. Varying dosages of Gui Zhi Tang administrated orally, i.p. and i.m. were able to inhibit the amounts of PFC, SRFC and the DTH response induced by BSA and the proliferation response of murine spleen cells to Con A and LPS. Further studies showed that Gui Zhi Tang had the inhibitory effect on Interleukin-2 production of murine spleen cells, which might be one of the mechanisms leading to the immunosuppressive effects of Gui Zhi Tang.