年轻恒牙牙根发育过程中牙髓中Ⅲ型胶原的表达

目的：研究牙根发育不同阶段的人年轻恒牙牙髓中Ⅲ型胶原的表达变化：方法：根据牙根的发育情况，分为牙根刚开始发育、牙根发育中和根尖闭合共3组：采用SP免疫组化法。对各组牙髓标本的石蜡切片进行Ⅲ型胶原的免疫组化染色：结果：图像分析显示、3个组的冠髓中心染色强度之间有非常显著性差异，以牙根刚开始发育时染色最强，根尖闭合时染色最弱：在牙根刚开始发育组、牙髓中心区染色强于牙髓外层。结论：随着年轻恒牙牙根的逐渐发育，Ⅲ型胶原在牙髓中的表达逐渐减弱，因而可能参与牙根的发育。     

牙色度分析在原位乳牙脱矿与再矿化检测中的应用

目的:验证数码照相牙色度分析法进行体内乳牙脱矿与再矿化研究的可行性。方法:选取58例平均年龄为4岁的儿童,以左上中切牙为实验牙,先染色、数码拍照,然后对实验牙面进行酸蚀、染色、数码拍照,最后在口腔环境内自然再矿化24h和1周后再染色、拍照,运用图像分析软件进行牙色度R、G、B值分析,比较酸蚀脱矿及再矿化前后牙色度的变化。用SPSS 19.0软件包对数据进行方差分析。结果:酸蚀脱矿后及再矿化24h后的色值(R、G、B)与基值有显著差异(P<0.05);在1周时间点上,只有B值与基值有显著差异(P<0.05);再矿化24h及1周后,牙色度值与酸蚀后相比有显著差异。结论:数码牙色度分析法可识别乳牙脱矿及再矿化前后牙色度的变化,可用于原位乳牙脱矿与再矿化研究。     

Carnoy液对金黄地鼠颊囊作用的研究

目的了解Carnoy液对粘膜组织损伤的性质，为其用于临床治疗提供理论依据。方法以金黄地鼠颊囊作为模型，以Carnoy液作为涂布试剂，药液作用时间为4min，于术后不同时期处死动物，切取标本，进行光镜和透射电镜观察。结果Carnoy液可引起颊囊粘膜上皮坏死脱落，溃疡形成，穿透深度约0.3mm。术后第3周坏死区上度重新形成，胶原纤维及成纤维细胞明显增多。结论Carnoy液是一种烧灼性质较强的物质，将其用于上皮性囊肿治疗，可因上皮破坏而治愈囊肿或降低其复发率。     

'Love Teeth Day' campaign in China and its impact on oral public health - the twentieth anniversary

The date 20 September has been designated 'Love Teeth Day' (LTD) in China since 1989. The aim of this nationwide campaign is to encourage all Chinese people to conduct preventive oral public health care and promote oral health education; therefore it is beneficial to improve the levels of oral health in the whole Chinese population. Public awareness of oral health in China has substantially improved following 20 years of hard work from dental professionals and relevant departments. The main activities were planned and conducted by the National Committee for Oral Health and local committees at provincial, county and municipal levels to support preventive oral care. The LTD campaign attracts more and more attention from the public on the prevention and control of oral diseases, which is considered to be an extremely pioneering health care programme in the field of Chinese dentistry. This review provides an overview of the huge LTD campaign over the past 20 years, aiming to share our experiences with international dental professionals.     

功能矫治器矫治安氏Ⅱ类Ⅰ分类错He的软组织侧貌变化

目的 用5项软组织美学指标，评价功能矫治器矫治安氏类Ⅰ分类错殆的软组织变化。方法 对21例安氏类Ⅰ分类错He患者用功能矫治器(FR-2)矫治前后的X线头影测量片进行分析，对比治疗前后软组织5项美学指标的变化。结果 通过早期的功能矫形治疗，患者大多数美学指标接近正常值范围，但也有部分指标改善不明显。结论 功能矫治能使大多数患者软组织侧貌得到明显改善，但存在个体差异，部分病例仍需要二期治疗。     

Cervical Castleman disease in children

We report a new case of cervical Castleman disease (hyaline vascular type) in a 6-year-old child. The mass was shown on computed tomography and magnetic resonance angiography (MRA) to be compressing the bifurcation of the right common carotid artery. At operation four masses were found, which were tightly adherent to both the artery and the right jugular vein. These were removed by blunt dissection and there were no signs of recurrence after 6 months. This is a rare tumour (we know of only 24 other reports), which is usually asymptomatic, and of uncertain prognosis.     

婴幼儿血管瘤继发组织坏死的临床研究

本文通过对65例血管瘤中的5例继发组织坏死的血管瘤进行血清雌二醇水平(E2)测定、肥大细胞计数,发现其血清E2水平及肥大细胞计数都明显高于正常对照组。经强的松及平阳霉素治疗后坏死的溃疡面愈合,并且血管瘤体积缩小,颜色明显变浅。     

全下颌种植固定义齿三维各向异性有限元模型的建立

种植义齿的远期成功率与其生物力学相容性关系密切。本文采用CT扫描数字探测法和图形图像处理分析系统，在微机上完成全下颌种植固定义齿三维空间坐标数据测量，在Var-6510计算机上采用ANSYS有限元分析程序的布尔运算和单元自动划分功能建立全下颌种植固定义齿的三维各向异性有限元模型；并在咀嚼肌附着区和颞颌关节区模拟口腔咀嚼状况时加约束反力，建立生理状况下的下颌有限元模型。     

雷帕霉素联合顺铂对CCL19调节头颈鳞状细胞癌细胞活性的影响

目的 探讨雷帕霉素(rapamycin)与顺铂(cisplatin)联合使用对趋化因子CCL19调节头颈鳞状细胞癌细胞活性的影响.方法 应用甲基噻唑基四唑(MTT)法及流式细胞仪检测顺铂及雷帕霉素对CCL19诱导后人头颈鳞状细胞癌淋巴结转移细胞系(PCI-4B、PCI-37B)生长抑制作用、细胞凋亡作用和细胞周期变化.使用Calcusyn软件计算两种药物(浓度比为80:1)联合使用时的剂量效应参数及联合指数(combination index,CI),使用SPSS 11.0软件包进行统计分析.结果 顺铂及雷帕霉素可以分别提高CCL19作用后细胞的生长抑制率(CCL19+顺铂作用后两细胞系的生长抑制率为21.22%±2.68%和22.76%±2.79%,CCL19+雷帕霉素作用后为19.46%±2.54%和20.6%±4.38%)、细胞G1期比例(CCL19+顺铂作用后两细胞系的G1期比例为72.14%±2.66%和76.39%±1.45%,CCL19+雷帕霉素作用后为75.26%±5.92%和74.00%±1.51%)和凋亡率(CCL19+顺铂作用后两细胞系的凋亡率9.59%±0.86%和7.55%±0.66%,CCL19+雷帕霉素作用后为8.21±1.80%和6.26±0.78%).两种药物浓度比为80:1联合使用时,PCI-4B细胞在半数抑制量(inhibitory concentration 50%,IC50)以下,CI＜1,IC75以上CI＞1;PCI-37B则在IC75以下CI＜1,IC90以上CI＞1.结论 顺铂及雷帕霉素均有抑制CCL19对人头颈鳞状细胞癌淋巴结转移细胞的活性调节作用,并且二者联合使用存在协同作用.

Abstract：

Objective To investigate the synergistic effects of rapamycin and cisplatin on head and neck squamous cancer cells regulated by chemokine( C-C motif) ligand 19 (CCL19). Methods The role of rapamycin and cisplatin was detected on cell-cycle and apoptosis in CCL19 induced PCI-4B and PCI-37B cells by methyl thiazolyl tetrazolium (MTT) and flow cytometry(FCM). Dose-effect relationship parameters and combination index(CI) were calculated on the median-effect equation and multiple drug effect equation using computer software CalcuSyn. Statistical analysis was performed by the unpaired student's t-test.Results Rapamycin and cisplatin could respectively increase the growth arrest, the proportion of G1 phase and apoptosis of CCL19 induced cancer cells (P ＜0. 05 ). Under inhibitory concentration 50% (IC50), CI was less than 1, and in IC75, it was more than 1 in PCI-4B cells. In PCI-37B cells, under IC75, CI was less than 1, and in IC90, it was more than 1. Conclusions Rapamycin and cisplatin can inhibit CCL19-regulated PCI-4B and PCI-37B cells' survival. The two drugs have synergistic effects when used in combination.