Smac在Ⅰ～Ⅱ期非小细胞肺癌组织中的表达及其临床意义

背景与目的：Smac是新近发现的一种由线粒体释放的促凋亡蛋白,主要作用于凋亡抑制蛋白（inhibitor of apoptosis proteins）．促进凋亡的发生：Smac在体外实验中显示出抗肿瘤作用,但在肺癌中其作用尚不明确。本研究通过检测Smac蛋白在Ⅰ～Ⅱ期非小细胞肺癌的表达,探讨其与临床病理因素及预后的关系。方法：采用组织芯片和免疫组化方法,对213例Ⅰ、Ⅱ期非小细胞肺癌肿瘤组织中Smac蛋白的表达情况进行检测。结果：Ⅰ、Ⅱ期NSCLC的5年生存率分别为61．9％和30．0％。Smac蛋白主要定位于细胞浆,以75％的阳性细胞数为分界点时,低表达组占60．6％。高表达组占39．4％。Smac的表达与性别、年龄、血型、病理类型及生存无相关性（P〉0．05）,但Smac蛋白在无淋巴结转移和有淋巴结转移的表达分别为37．0％和58．3％．二者比较有统计学意义（P〈0．05）。结论：Smac蛋白与Ⅰ～Ⅱ期非小细胞肺癌的淋巴结转移有关。     

CpG岛甲基化表型及OPCML基因甲基化与肝细胞癌发生的关系

背景与目的：CpG岛甲基化表型（CpG island methylator phenotype,CIMP）涉及到多个基因启动子同时甲基化,具有肿瘤特异性,与多种肿瘤的发生或预后相关。但有关肝癌CPG岛甲基化表型的研究罕见报道。OPCML（opioid-binding protein／cell adhesion molecule—like）基因目前多为针对上皮性卵巢癌的研究．被认为是卵巢癌的候选抑癌基因。本研究旨在探讨CIMP及OPCML基因与肝癌的发生是否有关。方法：运用甲基化特异性PCR方法检测50例肝细胞癌组织及48例癌旁组织中OPCML、p15、SOCS-1、GST-P、RAR．b、p16、p73、p14、MGMT和hMLHl基因的甲基化状况。结果：肝癌组织甲基化率普遍比相应癌旁组织甲基化率高：OPCML（70．0％VS．64．6％）、p15（58．0％VS．50．0％）、SOCS-1（78．0％VS．50．0％）、GST—P（56．0％VS．27．1％）、RAR-b（30．0％vs．6．3％）、p16（26．0％vs．14．6％）、p73（16．0％vs．0％）、p14（36．0％vs．27．1％）、MGMT（16．0％vs．10．4％）和hMLH1（18．O％VS．4．2％）。SOCS-1,GST-p,RAR-b,p16和p73基因甲基化率在肝癌组与癌旁组差异有显著性（P〈0．05）,其它基因两组之间的甲基化率差异无显著性。CIMP阳性组（同时具有≥3个位点甲基化）复发时间较早,1年无瘤生存率为18．2％,而CIMP阴性组（具有〈3个位点甲基化）复发时间较晚,1年无瘤生存率为75．0％（P〈0．05）。结论：肝癌中存在着CpG岛甲基化表型（CIMP）。CIMP可作为肝癌患者预后判断的指标之一：OPCML基因甲基化可能在肝癌的发生中发挥重要的作用。     

Two new phenolic amides from Allium chinenseOA

Objective: To isolate the phenolic amides from the dried bulbs of Allium chinense and investigate their myocardium protective activities.Methods: The chemical constituents were isolated and purified by combining with silica gel column,Sephadex LH-20 column, HPLC and other chromatography techniques. Their structures were elucidated by NMR techniques and mass spectrometry. The isolated compounds were evaluated to determine their protective effect for myocardium cells in vitro.Results: Two new phen...     

A rare case of purulent pericarditis secondary to subdiaphragmatic abscess

<正>Subdiaphragmatic abscess is the accumulation of pus in the space between the diaphragm and the transverse colon and its mesentery.^[1] Subdiaphragmatic abscess is clinically characterized by fever and local pain. Its clinical manifestations are often vague and diverse, and its symptoms and signs together constitute thoracoabdominal syndrome, leading to delayed diagnosis and a high incidence rate and mortality.^[2]Subdiaphragmatic abscess is often secondary to acute p...     

The effects of hyperbaric oxygen therapy on paroxysmal sympathetic hyperactivity after cardiopulmonary resuscitation: a case series

<正>The onset of cardiac arrest (CA) is sudden and critical.Due to cerebral ischaemia and hypoxia, the prognosis for post-cardiopulmonary resuscitation (CPR) patients is poor. Paroxysmal sympathetic hyperexcitability (PSH) is a potentially life-threatening condition, which is characterized by episodes of increased heart rate and blood pressure,sweating, hypothermia, and forced posture.[1] Hypoxicischaemic encephalopathy post-CPR can lead to PSH,which often indicates a worse prognosis. Curre...     

Combination therapy to overcome ferroptosis resistance by biomimetic self-assembly nano-prodrug

Ferroptosis has emerged as a potent form of no-apoptotic cell death that offers a promising alternative to avoid the chemoresistance of apoptotic pathways and serves as a vulnerability of cancer. Herein, we have constructed a biomimetic self-assembly nano-prodrug system that enables the co-delivery of gefitinib(Gefi), ferrocene(Fc) and dihydroartemisinin(DHA)for the combined therapy of both ferroptosis and apoptosis. In the tumor microenvironment,this nano-prodrug is able to disassemble and trig...     

Nutritional status and prognostic factors for mortality in patients admitted to emergency department observation units: a national multi-center study in China

BACKGROUND: Nutritional risk is common among patients admitted to the emergency department and is associated with adverse clinical outcomes. Despite its large population, few comprehensive studies have been conducted in China concerning the nutritional status of patients admitted to emergency department observation units(EDOUs).METHODS: Patients admitted to EDOUs of 90 tertiary hospitals in China between June 2020 and December 2020 were enrolled. Demographic information, laboratory parameters, n...     

The influence of pressure injury risk on the association between left ventricular ejection fraction and all-cause mortality in patients with acute myocardial infarction 80 years or older

BACKGROUND: We aimed to investigate whether the pressure injury risk mediates the association of left ventricular ejection fraction(LVEF) with all-cause death in patients with acute myocardial infarction(AMI) aged 80 years or older.METHODS: This retrospective cohort study included 677 patients with AMI aged 80 years or older from a tertiary-level hospital. Pressure injury risk was assessed using the Braden scale at admission, and three risk groups(low/minimal, intermediate, high) were defined ac...     

Correlation between immune-related adverse events and long-term outcomes in pembrolizumab-treated patients with unresectable hepatocellular carcinoma: A retrospective study

BACKGROUND Although immune checkpoint inhibitor(ICI) therapy has improved the prognosis of unresectable hepatocellular carcinoma(HCC), it has also resulted in unique immune-related adverse events(irAEs). The relationship between irAE and treatment outcomes in ICI-treated unresectable HCC patients remains unknown.AIM To elucidate the correlation between immune-related toxic effects and prognosis in patients with unresectable HCC treated with pembrolizumab.METHODS From March 2019 to February 2021,...