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41.
42.
人外周血CD68^+单核细胞体外的分选及诱导分化为破骨细胞 总被引:7,自引:0,他引:7
OBJECTIVE: To establish a method for obtaining highly purified primary human osteoclast precursors for the biochemical and molecular biological research. METHODS: CD68(+) mono/macrophages were separated from peripheral blood mononuclear cells (PBMCs) of healthy donors by means of immunomagnetic cell sorting for subsequent analysis with flow cytometry. The isolated cells were incubated on coverslips or bone slices in the presence of dexamethasone(10(-8) mol/L), macrophage colony-stimulating factor (25 microg/L ) and soluble receptor activator of nuclear factor (NF)-kappaB ligand (s-RANKL, 16 microg/L). Calcitonin receptor (CR) immunocytochemistry and tartrate-resistant acid phosphatase (TRAP) histochemistry were employed. The bone slices were also studied by scanning electron microscopy (SEM). RESULTS: Fluorescence-activated cytometric analysis showed that 93.06%+/-0.61% n=4 of the selected cells were CD68(+) cells. After 7 days of culture of the CD68(+) cells, VR+, TRAP+ multinucleated giant cells appeared, and resorption lacunae could be observed by SEM. CONCLUSION: Highly purified CD68(+) cells can be obtained from human PBMCs as the osteoclast precursors, and mature osteoclasts can be induced from CD68(+) mono/macrophages by RANKL. 相似文献
43.
一个企业风光几年,在全世界都是一种普遍现象。在日本曾经做过统计,经营历史超过5年的企业不到20%,80%都在5年内消失了。然而创建于1992年12月18日的深圳太太药业股份有限公司,虽然是以保健品起家,多年来却一直保持着持续稳定的增长势头,成为行业的翘楚。1993年3月8日面市以来,太太口服液已连续畅销近十年,静心口服液上市三年来,销售也一直保持快速的增长态势,在2001年度销售更是势头迅猛,年销售额达21,656.62万元;同时,在OTC药品方面,太太药业陆续推出了意可贴、正源丹、汉林清脂等一系列产品,市场反应都极为积极,2000年推出的意可贴,更是用小药做出了大市场,赢得了非常理想的市场份额和患者口碑。2001年6月8日,深圳太太药业股份有限公司在上海证交所挂牌上市,并一举融资17亿,2002年4月3日太太药业股份有限公司发布公告,拟变更募集资金用途,用于收购健康药业“中国”有限公司100%股权及购买鹰牌商标物注册商标所有权。此次受让健康药业股权及鹰牌商标款共计折台人民币14,151万元。这是太太药业上市面上后首次的收购行动,也是资本在太太药业身上的首次说话。太太,已经变得越来越强大,用太太药业董事长朱保国的话说:低于10亿美元,别想买太太药业。那么,太太是如何取得今天的成就的呢? 相似文献
44.
目的 分析创伤性肝破裂外科多种干预治疗的效果。方法 总结分析1982-2002年我院收治创伤性肝破裂76例的救治方法和病死率。其中Ⅲ级以上的严重肝破裂46例(60.53%)。手术治疗56例,手术方式包括单纯修补、清创性肝切除、规则性肝切除、肝周填塞止血;非手术治疗20例。结果 手术组:治愈44例,术后并发症17例均经保守治疗治愈,死亡12例,其中术中死亡2例;非手术组:治愈12例,好转6例,2例失访。全组治愈64例,死亡12例,病死率15.8%。结论 Ⅰ-Ⅱ级单纯性外伤性肝破裂可保守治疗;手术是治疗创伤性肝破裂的主要措施,正确的手术方式,积极处理合并伤,重视综合治疗可提高救治成功率。 相似文献
45.
目的 探讨老年人肱骨近端二部分和三部分移位骨折的手术治疗方法。方法 回顾性分析19例老年肱骨近端二部分和三部分移位骨折患者的临床资料,其中二部分骨折13例、三部分骨折6例,采用切开复位,肱骨近端解剖钢板固定治疗。结果 二部分骨折术后优良率为76.9%,未出现骨折不愈合及肱骨头坏死;三部分骨折术后优良率为66.7%,肱骨头坏死率16.7%。结论 老年人肱骨近端二部分和三部分骨折应尽量采用有限切开复位,选择肱骨近端解剖钢板固定。 相似文献
46.
Inhibitory Effect of Angiogenesis Inhibitor TNP—470 on Human ACHN Renal Cell Carcinoma 总被引:2,自引:0,他引:2
Angiogenesis,the formation of new bloodvessels,is necessary for the growth of tumors andtheir metastasis.TNP- 470 is a synthetic analogueof fumagillin and has stronger antiangiogenic ac-tivity and less side- effects than fumagillin.Thiscompound has been reported to suppress the prolif-eration and metastasis of various kinds of tu-mors[1] . Using nude mice xenografts of ACHN hu-man renal cell carcinoma ( RCC) ,the presentstudyattempted to examine the action of TNP- 470 ongrowth,metastasi… 相似文献
47.
The promoting effect of the Chinese medicinal herb, Wikstroemia chamaedaphne and Tung oil extracts (WC and HHPA) on carcinoma of uterine cervix induced by HSV-2 or MCA in mice was studied. The results showed that WC and HHPA extracts were not carcinogenic themselves. After carcinogen HSV-2 and MCA treatment, WC and HHPA were added separately. The inducing rates by HSV-2 increased from 7.4% to 21.1% and 26.3%, those by MCA increased from 56.5% to 82.8% and 84.4%. There was a significant difference between the combined groups and groups with HSV-2 or MCA only. The experimental results suggest that these two kinds of extracts play a promoting effect on carcinogenesis. The relation between the carcinogenesis of uterine cervix or nasopharynx and WC or HHPA extracts is discussed. 相似文献
48.
本文采用小鼠精子试验和小鼠睾丸DNA合成抑制试验,检测了JX-1对雄性生殖细胞的致突变作用。实验结果表明JX-1对小鼠精子总数、精子活动率及精子畸形率均无统计学意义的增加,与小鼠睾丸DNA合成抑制试验阴性反应一致。作者认为JX-1对雄性生殖细胞为一种非诱变剂。 相似文献
49.
循证护理在糖尿病皮肤损害临床实践中的应用 总被引:15,自引:0,他引:15
目的 应用循证护理 (EBN) ,探索糖尿病皮肤损害有效干预方法 ,减少残疾及死亡率 ,提高病人生活质量。方法 通过临床评估确定护理诊断 ,查阅文献 ,选择最佳护理证据 ,制定护理干预措施。结果 通过治疗及护理干预 ,2 2例糖尿病患者皮肤损害 ,显效 2 1例 ,有效 1例 ,转科 1例 ,出院时患者无并发症。结论 循证护理应用于临床 ,使传统护理经验走向护理科学发展 ;促进护士主动学习 ,提高护士综合素质 ;优质护理服务于个体化 ;解决患者现存的、潜在的护理问题 ,提高患者生存质量。 相似文献
50.
Synergistic effects of Nell-1 and BMP-2 on the osteogenic differentiation of myoblasts. 总被引:1,自引:0,他引:1
Catherine M Cowan Xinquan Jiang Tiffany Hsu Chia Soo Beiji Zhang Joyce Z Wang Shun'ichi Kuroda Benjamin Wu Zhiyuan Zhang Xinli Zhang Kang Ting 《Journal of bone and mineral research》2007,22(6):918-930
Osteogenesis is synergistically enhanced by the combined effect of complimentary factors. This study showed that Nell-1 and BMP-2 synergistically enhanced osteogenic differentiation of myoblasts and phosphorylated the JNK MAPK pathway. The findings are important because of the osteochondral specificity of Nell-1 signaling and the potential therapeutic effects of coordinated BMP-2 and Nell-1 delivery. INTRODUCTION: BMPs play an important role in the migration and proliferation of mesenchymal cells and have a unique ability to alter the differentiation of mesenchymal cells toward chondrogenic and osteogenic lineages. Signaling upstream of Cbfa1/Runx2, BMPs effects are not limited to cells of the osteoblast lineage. Thus, additional osteoblast-specific factors that could synergize with BMP-2 would be advantageous for bone regeneration procedures. NELL-1 (NEL-like molecule-1; NEL [a protein strongly expressed in neural tissue encoding epidermal growth factor like domain]) is a novel growth factor believed to preferentially target cells committed to the osteochondral lineage. MATERIALS AND METHODS: C2C12 myoblasts were transduced with AdLacZ, AdNell-1, AdBMP-2, or AdNell-1+AdBMP-2 overexpression viruses. Effects were studied by cell morphology, alkaline phosphatase activity, osteopontin production, and MAPK signaling. Additionally, in a nude mouse model, viruses were injected into leg muscles, and new bone formation was examined after 2 and 8 wk. RESULTS: C2C12 myoblasts co-transduced with AdNell-1+AdBMP-2 showed a synergistic effect on osteogenic differentiation as detected by alkaline phosphatase activity and osteopontin production. Nell-1 stimulation on AdNell-1 + AdBMP-2 preconditioned C2C12 cells revealed significant activation of the non-BMP-2 associated c-Jun N-terminal kinase (JNK) MAPK signaling pathway, but not the p38 or extracellular signal-regulated kinase (ERK1/2) MAPK pathways. Importantly Nell-1 alone did not induce osteogenic differentiation of myoblasts. In a nude mouse model, injection of AdNell-1 alone stimulated no bone formation within muscle; however, injection of AdNell-1+AdBMP-2 stimulated a synergistic increase in bone formation compared with AdBMP-2 alone. CONCLUSIONS: These findings are important because of the confirmed osteochondral specificity of Nell-1 signaling and the potential therapeutic effects of enhanced BMP-2 action with coordinated Nell-1 delivery. 相似文献