微矩阵基因芯片筛选喉鳞癌相关基因的研究

目的：应用基因微矩阵方法筛选喉鳞癌相关基因。方法：按一步法抽提喉鳞癌和对照喉正常组织的总ＤＮＡ并纯化ｍＲＮＡ;将４０９６种人类基因ＰＣＲ产物用ＣａｒｔｅｓｉａｎＰｉｘｓｙｓ７５００点样仪按微矩阵排列点样于化学涂层的载玻片上,制成基因芯片;将等量的对照组织和喉鳞癌组织ｍＲＮＡ分别逆转录合成荧光分子掺入的ｃＮＤＡ－链做探针,混合后杂交上述基因芯片,经严格洗片后用ＳｃａｎＡｒｒａｙ３０００扫描仪扫描芯片     

神经细胞粘附分子在人脑星形细胞瘤中的表达

研究神经细胞粘附分子在人脑星形细胞瘤发生发展中的作用。方法：用原位杂交和免疫组织化学方法检测４０例人脑星形细胞瘤组织中的ＮＣＡＭｍＲＮＡ及其蛋白（ＣＤ５６）的表达。结果：星形细胞瘤１,２级中ＮＣＡＭｍＲＮＡ及蛋白的表达显著高于星形细胞瘤３,４级,核酸与蛋白水平的检测结论一致。结论：ＮＣＡＭｍＲＮＡ及其蛋白的表达与星形细胞瘤的恶性程度有关,ＮＣＡＭ的高表达可与星形细胞瘤的侵袭转有关,并有可能成为星形     

L-硝基精氨酸甲酯对实验性葡萄膜炎的影响

目的：观察一氧化氮（ＮＯ）含量含量在葡萄炎房东水及玻璃体中的变化及Ｌ－硝基精氨酸甲酯（Ｎ＾Ｇ－ｎｉｔｒｏ－Ｌ－ａｒｇｉｎｉｎｅ ｍｅｔｈｙｌ ｅａｒｔｅｒ,Ｌ－ＮＡＭＥ）的治疗效果。方法;采用兔眼玻璃２本内注射内毒素脂多糖（ＬＰＳ）制备葡萄膜炎模型,以检测房水蛋白浓度,结果：葡萄为房水及玻璃体及玻璃体中显著增高,即刻使用Ｌ ＮＡＭＥ;显著降低了ＮＯ水平,同时其蛋白浓度、细胞数目及组织炎症程度明显降     

冻干人硬脑膜用于修补硬脑膜缺损和血管内栓塞治疗的临床观察

目的：探讨用冻干人硬脑膜行硬脑膜成硬脊膜缺损修补和血管内栓塞治疗的效果。方法：用冻干人硬脑膜修补因脑肿瘤、脑外伤、脊髓内肿瘤手术引起的硬脑膜或硬脊膜缺损,并不同规格的的冻干人硬脑膜微粒或微丝行靶血管栓塞。结果：（１）冻干人硬脑膜用于硬脑或硬脊膜缺损修补患者２２９例,无颅仙感染等并发症,用于血管内栓塞靶血管２８例,可明显减少或闭塞颅内肿瘤的血供。结论：冻干人厝脑膜是一种较为理想的硬脑膜缺损修补和血管     

检测孕妇血浆中的urocortin

目的：研究ｕｒｏｃｏｒｔｉｎ（促肾上腺皮质激素释放激素肽类家族新成员）是否存在于孕妇血循环中及其含量,以证实ｕｒｏｃｏｒｔｉｎ是否能在妊娠中产生并由此调节子宫－胎盘的血管张力。方法：用放射免疫测定法从受孕１６周起检测孕妇血浆中是否含有ｕｒｏｃｏｒｔｉｎ,并用凝胶层析法检测从孕妇血浆中提取的ｕｒｏｃｏｒｔｉｎ是否与人工合成的ｕｒｏｃｏｒｔｉｎ标准品一致。结果：从受孕１６周起孕妇的血浆中即可测得ｕｒｏ     

喉癌中人乳头状瘤病毒感染的检测及其临床分析

目的：探讨喉鳞状细胞癌中人乳头状瘤病毒（ＨＰＶ１６,１８亚型）感染情况及临床分析。方法：用免疫组化的方法对４２例喉鳞癌组织中ＨＰＶ１６,１８亚型情况进行检测。结果：４２例喉鳞癌组织中ＨＰＶ１６,１８亚型感染阳性率６４．２９％（２７／４２）,但ＨＰＶ１６,１８感染与临床分期、癌细胞的分化程度、肿瘤的淋巴结转移和临床分型无关。结论：ＨＰＶ１６,１８感染可能是人喉癌形成的触发因素,它对肿瘤生物学行为的影     

盐酸维拉帕米脉冲控释片人体内的药代动力学及生物利用度研究

目的：比较盐酸维拉帕米脉冲释放片与其片芯在人体的药代动力学特征及生物利用度。方法：采用拉丁方设计,将志愿者８人随机分为３组,交叉口服Ⅲ,Ⅳ型脉冲片和片芯,以片芯为对照制剂,考察体内脉冲控释效果。结果：Ⅲ型脉冲片在体内的时滞为４ｈ,而ｃｍａｘ,ＡＵＣ等与片芯无明显差异,达到设计要求。结论：该研究制备的脉冲释放制剂为防治心血管疾病的凌晨发作提供了一个良好的剂型选择。     

Ultraviolet blood irradiation and oxygenation affects free radicals and antioxidase after rabbit spinal cord injury

Ｕｌｔｒａｖｉｏｌｅｔｂｌｏｏｄｉｒｒａｄｉａｔｉｏｎａｎｄｏｘｙｇｅｎａｔｉｏｎ (ＵＢＩＯ)ｉｓａｆｏｒｍｏｆｐｈｙｓｉｃａｌｔｈｅｒａｐｙ ,ｄｕｒｉｎｇｗｈｉｃｈａｃｅｒｔａｉｎｖｏｌｕｍｅｏｆｖｅｎｏｕｓｂｌｏｏｄｉｓｄｒａｗｎｆｒｏｍａｐａｔｉｅｎｔａｎｄｐｒｏｃｅｓｓｅｄｗｉｔｈａｓｐｅｃｉｆｉｃｄｏｓｅｏｆｕｌｔｒａｖｉｏｌｅｔｉｒｒａｄｉａｔｉｏｎａｎｄｏｘｙｇｅｎａｔｉｏｎｉｎａｂｌｏｏｄｔｈｅｒａｐｙｉｎｓｔｒｕｍｅｎｔ,ｉｔｉｓｔｈｅｎｉｎｆｕｓｅｄｂａｃｋｉｎｔｏｔｈｅｓａｍ…     

Gene polymorphism in apolipoprotein E and presenilin-1 in patients with late -onset Alzheimer’s disease

Objective To evaluate the association of apolipoprotein E (apoE) and presenilin-1 (PS-1) gene polymorphism with late-onset Alzheimer’s disease (AD). Methods A case-control study was undertaken to detect the polymorphism of apoE and PS-1 by polymerase chain reaction and digestion with the endonucleases of BspL Ⅰ, Hha Ⅰ and BamH Ⅰ. Results The frequencies of apoE ε3/4 genotype and ε4 allele in late-onset AD (n=42) were significantly higher than those of age-matched controls (P<0.05). The frequencies of the apoE intron 1 enhancer (IE1) G/G genotype and G allele in late-onset AD were also significantly higher than those in controls (P<0.05). The frequencies of the PS-1 1/1 genotype but not the 1 allele in AD were significantly higher than those in controls (P<0.05).The apoE ε4 allele was associated with a tripling of risk for late-onset AD compared with that with no ε4 allele (odds ratio: 2.932). Homozygosity of the G allele in IE1 and 1/1 genotype in PS-1 was associated with a doubling of risk for late-onset AD, and odds ratios were 2.223 and 2.066, respectively.When the apoE ε4 was controlled, the association between the IE1 G/G genotype AD was no longer statistically significant (P>0.05). We sequenced the exon 4 of apoE in patients with late-onset AD, and found no other genetic polymorphism or mutation except for apoE ε4 and IE1 G alleles associated with AD. Conclusion apoE ε4 gene appears to be the strongest gene risk factor for late-onset AD and its apparent association between the IE1 G/G genotype and late-onset AD is a consequence of the association between the ε4 and IE1 G/G genotype.The PS-1/1 genotype is weakly associated with late-onset AD.