两种纳米晶羟基磷灰石的细胞相容性比较

目的 比较两种不同形态纳米晶羟基磷灰石的细胞相容性。方法 采用化学湿法合成的两种纳米晶形态羟基磷灰石。采用原代培养的成骨细胞检测两者的细胞相容性。采用MTT法观察成骨细胞在几种材料表面3d后的增殖情况，细胞培养第5d时对碱性磷酸酶活性进行检测，并用SEM观察第3d时细胞在材料表面的附着形态。结果 成骨细胞在球形纳米晶羟基磷灰石表面的细胞增殖数显著高于同直径的棒状纳米晶羟基磷灰石，并具有更好的细胞伸展形态；而碱性磷酸酶表达没有明显差别。结论 羟基磷灰石球形纳米晶较棒状纳米晶更能促进成骨细胞生长。     

硬衬材料中甲基丙烯酸酯单体释放的实验研究

目的 测定两种硬衬材料聚合后丙烯酸酯单体的释放量和趋势.方法 采用顶空固相微萃取气相色谱法测定两种常用的硬衬材料聚合后不同时段丙烯酸酯单体释放量.结果 两种硬衬材料的丙烯酸酯单体在10 min时释放量即达到释放总量的60%, 30 min时趋于稳定.在30 min时德山rebaseⅡ型丙烯酸酯单体释放量为0.003 9 mg/g,YJ-F自凝仿生义齿基托树脂为0.509 mg/g.结论 硬衬材料聚合后丙烯酸酯单体的释放随时间增加而逐渐下降,在30 min后趋于稳定.     

近距离腔内放射治疗控制口腔癌术后复发的临床评价

目的:评价口腔癌术后近距离腔内照射控制复发的疗效及影响因素、临床应用价值.方法:口腔癌术后配合放射治疗患者238例,其中143例单独给予近距离腔内照射,95例同时给予近距离腔内照射和外照射.100例同期仅行手术治疗的病例作疗效对比.计算生存率和局部控制率.结果:手术治疗、术后配合腔内照射或同时配合腔内及外照射患者的5年和10年生存率分别是62%和22%,79%和62%以及71%和43%,3种治疗方法对肿瘤的5年和10年局部控制率分别是54%和52%,78%和76%以及72%和69%.UICC分期、病理分级、组织来源及肿瘤发生部位对生存率和局控率均有重要影响.结论:术后配合放疗可有效地控制口腔癌术后复发,尤以术后配合腔内照射更佳,该方法治疗后并发症少且疗效显著,可望成为新的、常规的口腔癌治疗手段.     

三峡库区5433例病残儿先天性心脏病流行病学调查

目的 探讨重庆三峡库区16个区县儿童先天性心脏病的发生情况,为先天性心脏病人群干预及病因学研究提供参考.方法 利用重庆市病残儿医学鉴定平台,收集、回访、调查、整理三峡库区所属区、县5433例病残儿医学鉴定资料并从中筛选出418例先天性心脏病患儿,分析先天性心脏病与库区环境因素的关系.结果 大渡口区、涪陵区、长寿区、沙坪坝区、巴南区、万州区等儿童先天性心脏病发病率较高,奉节县等区县人群的患病率较低.418例先天性心脏病患儿中,室间隔缺损最多(44.7％),其他依次为房间隔缺损(10.8％)、动脉导管未闭(8.9％)、法洛四联征(7.7％)等.结论 室间隔缺损为重庆三峡库区先天性心脏病的主要类型,加大早期筛查力度,加强孕早期保健和防护,重视产前健康筛查,对预防儿童先天性心脏病具有较大意义.     

Inverted U-Shaped Associations between Glycemic Indices and Serum Uric Acid Levels in the General Chinese Population: Findings from the China Cardiometabolic Disease and Cancer Cohort (4C) Study

Objective The relationship between serum uric acid(SUA)levels and glycemic indices,including plasma glucose(FPG),2-hour postload glucose(2 h-PG),and glycated hemoglobin(HbA1 c),remains inconclusive.We aimed to explore the associations between glycemic indices and SUA levels in the general Chinese population.Methods The current study was a cross-sectional analysis using the first follow-up survey data from The China Cardiometabolic Disease and Cancer Cohort Study.A total of 105,922 community-dwelling adults aged≥40 years underwent the oral glucose tolerance test and uric acid assessment.The nonlinear relationships between glycemic indices and SUA levels were explored using generalized additive models.Results A total of 30,941 men and 62,361 women were eligible for the current analysis.Generalized additive models verified the inverted U-shaped association between glycemic indices and SUA levels,but with different inflection points in men and women.The thresholds for FPG,2 h-PG,and HbA1 c for men and women were 6.5/8.0 mmol/L,11.0/14.0 mmol/L,and 6.1/6.5,respectively(SUA levels increased with increasing glycemic indices before the inflection points and then eventually decreased with further increases in the glycemic indices).Conclusion An inverted U-shaped association was observed between major glycemic indices and uric acid levels in both sexes,while the inflection points were reached earlier in men than in women.     

Full-Length Genome Sequencing of SARS-CoV-2 Directly from Clinical and Environmental Samples Based on the Multiplex Polymerase Chain Reaction Method

The new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is spreading worldwide with the number of confirmed cases increasing dramatically[1,2]. Coronaviruses have the largest genome among RNA viruses and have caused two major outbreaks[3,4]. The new coronavirus (SARS-CoV-2) is the third coronavirus spread globally in the past 17 years. According to the World Health Organization, SARS-CoV-2 has affected more than 155.6 millon patients in 222 countries as of 6 May 2021 and has become a major global health concern (https://www.who.int/emergencies/diseases/novel-coronavirus-2019).     

National Nutrition and Health Systematic Survey for Children 0-17 Years of Age in China

The main purpose of the National Nutrition and Health Systematic Survey for children 0-17 years of age in China (CNHSC) was to collect basic data on the nutrition, development, and health status for children in different regions across China using evidence-based, reliable, and cost-effective approaches. Children and their parents or guardians from seven regions (south, southwest, north, northwest, eastern, central, and northeast China) in China were recruited. A multi-stage stratified randomized sampling method was used. Two provinces were randomly sampled from each of the seven regions, from which one urban district and one rural country were also randomly sampled, resulting in a total of 28 survey counties/districts. Dietary surveys, health examinations, laboratory testing, and questionnaires were used to collect dietary intake, nutritional status, child development, and health status information. Nutrition, health, and lifestyle assessment of children and their parents was determined using the Knowledge Attitude Practice (KAP) survey. Greater than 100,000 children (38,000 children < 6 years of age and 66,000 children 6-17 years of age) completed the survey. The survey provided comprehensive data on child nutrition and health status for future studies and will serve as the basis for an integrated nutrition and health improvement strategies proposal for children in China.     

Physical Activity and Sedentary Behaviors among Chinese Children: Recent Trends and Correlates

Objective This study was aimed at examining the trends and correlates of physical activity (PA) and sedentary behaviors among Chinese children. Methods A total of 4,341 subjects (6,936 observations) aged 6–17 years who participated in the China Health and Nutrition Survey (2004–2015) were included. Of the subjects, 41％ participated in the survey twice or more. Random-effects ordinal regression models and repeated-measures mixed-effects models were used to examine the PA trends. Quantile regression models were applied to examine the factors influencing PA and sedentary behaviors. Results From 2004 to 2015, the prevalence of physical inactivity among Chinese children aged 6–17 years increased by 5.5％ [odds ratio (OR), 1.51; 95％ confidence interval (CI), 1.19–1.90; P < 0.001]. The PA volume declined by 5.8 metabolic equivalent of task-hr/week (P < 0.001), and the time spent in sedentary behaviors increased by 1.8 hr/week (P < 0.001). Age, ethnicity, and region showed significant effects on the PA volume across the quartiles (P < 0.001). Across the quartiles, sedentary time was significantly higher in the children residing in urban areas (P < 0.001) or areas with high urbanization levels (P ≤ 0.005) than in their counterparts. Conclusions A declining PA trend among Chinese children aged 6–17 years was observed from 2004 to 2015, and certain subgroups and geographical areas are at higher risk of physical inactivity.     

Identification of Boseongicola sediminum sp. nov., a Novel Decabromodiphenyl Ether (BDE-209)-tolerant Strain Isolated from Coastal Sediment in Xiamen,China

Polybrominated diphenyl ethers (PBDEs) are a group of aromatic organic bromine compounds, which have been used in a broad array of polymeric materials (plastics, foams, resins, and adhesives) as brominated flame retardants. They are used in commercial and household products, including textiles, electronic equipment, airplanes, and automobiles, especially in China and the USA, because they are inexpensive with excellent flame-retardant effects[1]. During the past decade, over 14,000 tonnes of PBDEs have entered the market, and have become ubiquitous environmental contaminants[2].     

血清sRAGE、HbA1C表达与脓毒症患者应激性高血糖及其预后相关性

目的 探究血清可溶性晚期糖基化终末产物受体(sRAGE)、糖化血红蛋白(HbA1C)水平与脓毒症患者并发应激性高血糖(SHG)及其预后的关系。方法选取诊治的135例脓毒症患者为研究对象,按脓毒症患者发病24 h内是否发生SHG将其分为SHG组(56例)和正常血糖组(79例)。比较两组一般资料;应用酶联免疫吸附法(ELISA)检测血清sRAGE水平;采用高效液相色谱法检测血清HbA1C水平;比较两组患者28 d死亡率;比较不同预后脓毒症并发SHG患者血清sRAGE、HbA1C水平;利用受试者工作特征(ROC)曲线评估血清sRAGE、HbA1C水平对脓毒症并发SHG患者死亡的预测价值。结果与正常血糖组相比,SHG组患者急性生理与慢性健康评定量表Ⅱ(APACHE Ⅱ)评分、血清sRAGE、HbA1C水平、序贯器官衰竭评估(SOFA)评分、28 d死亡率均明显升高(P<0.05);与存活组相比,死亡组脓毒症并发SHG患者血清sRAGE、HbA1C水平均明显升高(P<0.05);血清sRAGE、HbA1C预测脓毒症并发SHG患者死亡的曲线下面积(AUC)分别为0.826、0.859,截断值分别为1.70 ng/mL、9.47%,相应敏感度分别为76.7%、73.3%,特异度分别为80.8%、84.6%;两者联合预测脓毒症并发SHG患者死亡的AUC为0.904,其敏感度、特异度分别为86.7%、80.2%。结论血清sRAGE、HbA1C水平升高均与脓毒症并发SHG患者不良预后关系密切,检测血清sRAGE、HbA1C水平有利于临床判定脓毒症并发SHG患者的预后。