Pathogenesis of congenital pyrimidine 5′ -nucleotidase | deficiency: A study on relationship among erythrocyte morphology, enzyme protein content and activity

Objective: To further explore the mechanism of congenital pyrimidine 5'-nucleotidase I deficiency. Methods; The samples were collected from the family members of a patient with P5'N- I deficiency. The enzyme activities were measured by UMP method and the enzyme proteins were quantified by ELISA while the morphology of peripheral blood cells was observed. Results: The enzyme contents reduced as their enzyme activities decreased in the family especially in four members. There was a significant positive correlation(r =0. 955) between the activity and the content of P 5'N- I . The count of the stippling cell was varied in the family. Conclusion.- One of the reasons for congenital P5' N- I deficiency might be the deficiency in the enzyme content. The morphology of peripheral blood erythrocyte may be an assistant diagnotic index. The P5'N- I activities and contents were measured simultaneously may be a effective method in clinic diagnosis.     

聚合酶链反应检测铜绿假单胞菌感染性角膜溃疡的实验研究及临床应用

目的：探索聚合酶链反应（PCR）技术对铜绿假单胞菌感染性角膜溃疡快速诊断的可行性。优越性及其在临床应用中的价值。方法：建立PCR检测标准铜绿假单胞菌方法，对兔铜绿假单胞菌感染性角膜溃疡模型研究并应用于临床，并与细菌培养做比较。结果：铜绿假单胞菌扩增出一条长504bp的阳性条带，其他常见细菌，人和兔正常角膜组织均为阴性，动物模型PCR敏感性为93.8%。特异性为100.0%；细菌培养敏感性为68.7%。特异性为93.7%。临床标本PCR阳性率为66.7%；细菌培养阳性率为38.1%。结论：PCR技术对快速检测实验室和临床标本中的铜绿假单胞菌具有重要的应用价值。     

云南白药对全髋关节置换术出血量的影响

目的研究云南白药在围术期对全髋关节置换术术中出血量及术后引流量的影响。方法2006至2008年我科收治的股骨颈骨折行全髋关节置换术60例,随机分成两组,分别服用云南白药胶囊和空胶囊;术前术后测定凝血酶原时间(PT),记录全部病人术中出血量及术后24 h引流量。结果实验组和对照组病人的凝血酶原时间(PT)差异有统计学意义(P<0.01);试验组的术中出血量、术后24 h引流量分别为(380±78)ml(、336±77)ml,较对照组(481±77)ml(、418±68)ml明显减少,差异具有统计学意义(P<0.01)。结论云南白药胶囊能够明显减少全髋关节置换术围术期出血量。     

浙江省2007～2008年突发公共卫生事件分析

目的 了解浙江省2007～2008年突发公共卫生事件的发生现状,为突发公共卫生事件防制提供依据.方法 应用描述流行病学方法对浙江省卫生厅公布的2007～2008年突发公共卫生事件发生信息数据、资料进行统计分析.结果 2007～2008年全省共发生突发公共卫生事件350起,累计发病人数41 553人,死亡22人,死亡率0.05%.事件种类以传染病和食物中毒为主,分别占86.3%和12.0%,事件程度以一般突发公共卫生事件为主,占96.6%.突发公共卫生事件好发于3～6月及9月.结论 为积极应对突发公共卫生事件,应健全应急体系机制,强化信息报送,开展监测预警,尤其是在每年3～6月份.     

急性大血管闭塞性轻型卒中血管内治疗的疗效及安全性观察

目的 观察急性大血管闭塞性轻型卒中患者血管内治疗的疗效及安全性。
方法 回顾性分析2018年1月-2019年5月行血管内治疗的急性大血管闭塞性轻型卒中（NIHSS评分
≤5分）患者的临床资料。观察术后血管成功再通率（mTICI≥2b级）、围手术期并发症、90 d良好预后率
（mRS评分≤2分）等。
结果 共纳入13例患者，平均年龄58.7±14.5岁，男性11例（84.6%）。术后血管成功再通率100%；围
手术期并发症2例，假性动脉瘤1例、无症状性颅内出血1例；90 d良好预后率100%。
结论 急性大血管闭塞性轻型卒中行血管内治疗可能是安全、有效的。     

胃癌大肠转移的X线钡灌肠表现

目的:分析胃癌大肠转移的X线钡灌肠表现。方法:104例胃癌入院术前检查发现结肠转移或胃癌术后转移入院治疗的病例。分析钡灌肠初次发现大肠转移征象的年龄、发现胃癌原发病变距初次发现大肠转移的间隔时间、转移病变的发生部位和病变造成的X线钡灌肠图像上的肠管变形和粘膜面的改变。结果:104例胃癌结肠转移,男67例,女37例。胃癌术前检查中发现结肠转移者32例,术后发现的大肠转移中,多数转移发生在手术后3年内(占91.3%)。胃癌原发灶的肉眼形态BorrmannⅢ型(28例)和BorrmannⅣ型(65例)者占89.4%,组织学中以低分化腺癌和印戒细胞癌为主要成分者占91.3%。X线钡灌肠检查显示大肠转移的好发部位为横结肠(80),其次为直肠(50)。转移灶可累及多节段肠管,其中横结肠中1/3段(47),横结肠左1/3(44),横结肠右1/3(39)和直肠腹膜返折之上(39)。X线显示肠管单侧变形者227处,双侧变形者96处。黏膜面表现为梳齿状黏膜纹聚集改变253处,颗粒结节状改变23处,外压性改变20处,弥漫性改变62处。结论:胃癌大肠转移的好发部位为横结肠和直肠,结肠黏膜面的梳齿状黏膜纹聚集为胃癌大肠转移的主要X线钡剂灌肠表现。     

平面导板在深覆(牙合)矫治中的临床应用研究

目的：探讨平面导板矫治重度深覆[牙合]患者的作用机制及临床作用要点。方法：对18名生长发育期的重度深覆[牙合]患者,用平面导板来打开咬合,并对咬合打开前后的硬软组织变化进行分析。结果：①上下后牙及牙槽高度均增加,下后牙及牙槽高度的增加值大于上后牙及牙槽高度的增加值,而对前牙及牙槽高度的影响无统计学差异;②上下颌骨在水平方向上无改变,但下颌平面角与下面高/全面高治疗后较治疗前增大,后面高/前面高治疗后较治疗前减小;③覆[牙合]减小,Spee氏曲线得到整平;④松弛的下唇肌肉得到紧张,从而改善了侧貌外形。结论：平面导板使覆[牙合]减小主要是后牙及后牙齿槽突高度增加的结果,对生长发育期的低角重度深覆[牙合]患者治疗效果较好。     

颞肌筋膜瓣与全厚皮片移植修复下睑凹陷性畸形

目的探讨颞筋膜瓣或颞肌筋膜瓣与自体全厚皮片移植,在下睑凹陷性瘢痕畸形修复中的应用效果。方法设计以颞浅动脉为蒂的颞筋膜瓣,对伴眶下壁骨缺损者同时行以颞浅、深动脉为蒂的颞肌筋膜瓣,并取耳后全厚皮片移植联合矫正下睑凹陷性畸形。结果8例患者,术后组织瓣及皮片成活良好,凹陷处填充效果满意。随访6个月至1年,睑外翻矫正,瘢痕不显,颞部供区毛发生长良好。结论应用颞筋膜瓣或颞肌筋膜瓣与全厚皮片移植,联合矫正下睑凹陷性畸形的方法,具有操作简便,且无明显的供区损害的特点,是修复下睑凹陷性瘢痕畸形较理想的方法。     

模糊聚类分析方法在髋关节图像处理中的应用

针对医学图像的模糊特点,结合模糊连接度,提出一种新的用模糊聚类分析检测圆弧的方法,它可以精确提取出圆弧.首先把曲线按一定长度进行分段处理,分别拟合出各段的圆心坐标和半径,通过模糊关系把具有一定相似性的聚为一类,把同一类中连续的段合在一起组成新段,再进行拟合,然后再对此段两端一定范围内寻找,直至找到最佳的圆弧.这种方法已应用到股骨头轮廓圆弧的提取.     

Uncooked rice of relatively low gelatinization degree resulted in lower metabolic glucose and insulin responses compared with cooked rice in female college students

Cooking processes that gelatinize granules or disrupt structure might increase the glucose and insulin responses because a disruption of the structure of starch by gelatinization increases its availability for digestion and absorption in the small intestine. We hypothesized that the uncooked form of rice, which has a relatively low degree of gelatinization even though in powder form, would result in lower metabolic glucose and insulin responses compared with cooked rice (CR). To assess the effects of the gelatinization of rice on metabolic response of glucose and insulin, we investigated the glucose and insulin responses to 3 rice meals of different gelatinization degree in female college students (n = 12): CR (76.9% gelatinized), uncooked rice powder (UP; 3.5% gelatinized), and uncooked freeze-dried rice powder (UFP; 5.4% gelatinized). Uncooked rice powders (UP and UFP) induced lower glucose and insulin responses compared with CR. The relatively low gelatinization degree of UPs resulted in low metabolic responses in terms of the glycemic index (CR: 72.4% vs UP: 49.7%, UFP: 59.8%) and insulin index (CR: 94.8% vs UP: 74.4%, UFP: 68.0%). In summary, UPs that were less gelatinized than CR induced low postprandial glucose and insulin responses.