转移瘤致股骨近端病理骨折的治疗

目的　探讨转移瘤致股骨近端病理骨折的治疗方法。方法　分析 1995年 5月至 2 0 0 2年 5月手术治疗的 16例的临床资料。结果　无手术并发症 ,术后疼痛明显缓解 ,伤口除 1例浅层感染外 ,余愈合良好。所有病例均存活在 3个月以上 ,14例术后继续原发肿瘤治疗。结论　股骨近端转移瘤所致的病理骨折应积极行手术治疗 ,内固定以带锁髓内钉加骨水泥为好。     

Electrophysiological study on differentiation of rat bone marrow stromal stem cells into neuron-like cells in vitro by edaravone

To explore the electrophysiological proper-ties of differentiation of rat bone marrow-derived stromal stem cells (rBMSCs) to neuron-like cells in vitro by edaravone, a new type of free radical scavenger. Methods: Stromal stem cells were separated from rat bone marrow with Ficoll-Paque reagent and expanded in different culture medium in vitro, rBMSCs were induced by edaravone containing serum-free L-DMEM. Morphologic observation and Western blot analysis including the ex-pression of Nav1.6, Kv1.2, Kv1.3, Cav1.2 were performed, and whole patch-clamp technique was used. Results: Cyton contraction and long processes were shown in differentiated stromal stem cells. Nav1.6, Kv1.2, Kv1.3 and Cav1.2 were expressed in both differentiated and undifferentiated cells. However, the expression of channel proteins in differentiated cells was up-regulated. Consistently, their resting potential and outward currents were also enhanced in the differentiated cells, which was especially significant in the outward rectifier potassium current. Conclusion: In vitro, neuron-like cells derived from rBMSCs, induced by edaravone, possess electrophysiologi-cal properties of neurons.     

单甲氧聚乙二醇化学修饰药物酶的研究进展

用单甲氧基聚乙二醉(1)化学修饰药物酶是生化药物研究开发的重要手段之一。本文综述了1化学修饰药物酶的一般方法及修饰后酶在生物和理化性质方面的变化，同时对1研究前景进行展望，并指出了尚待解决的问题。     

Effect of (+/-)-, (+)- and (-)-gossypol on the lactate dehydrogenase-X activity of rat testis

The effect of (+/-)-, (+)- and (-)-gossypol on testicular lactate dehydrogenase-X (LDH-X) was studied in vitro and in vivo. It was found that racemic gossypol and the two optical enantiomers had similar inhibitory effects on rat testicular LDH-X in vitro. However, neither racemic gossypol nor the enantiomers exhibited an inhibitory effect on testicular LDH-X in vivo. It is concluded that inhibition of testicular LDH-X is not likely to be the mechanism of the antifertility action of gossypol. The inhibition of testicular LDH-X in vitro by all three preparations of gossypol is probably non-specific.     

A comparison of the relative risk of vessel injury with conical versus pyramidal laparoscopic trocars in a rabbit model

OBJECTIVE: Our purpose was to compare the relative risk of vessel injury after use of a 5 mm conical-tipped trocar, a 5 mm pyramidal-tipped trocar, and a 10 mm pyramidal-tipped trocar in a rabbit vessel model.STUDY DESIGN: Plastic templates were placed in front of and behind 108 mesenteric vessels in 11 anesthetized New Zealand White rabbits. Laparoscopic trocars were inserted through the templates and mesentery. The incidence of vessel injury was determined at distances from the vessels ranging from 0 to 5 mm.RESULTS: The 5 mm conical trocar resulted in a vessel injury rate of 88% at 0 mm from the vessel but 0% at 1 or 2 mm. The 5 mm pyramidal trocar resulted in 100%, 88%, and 62% injury rates of 0, 1, and 2 mm from the vessels, respectively. The 10 mm pyramidal trocar resulted in a 100% injury rate at 0, 1, 2, or 3 mm from the vessels and 80% and 40% at 4 mm and 5mm, respectively.CONCLUSION: The relative risk of vessel injury is significantly increased by the use of pyramidal-tipped trocars when compared with conical-tipped trocars, especially if larger diameter trocars are used.     

脆性X综合征的遗传学诊断与产前诊断

脆性X综合征( fragile X syndrome,FraX )是一种常见的遗传性智力低下综合征,其发病率仅次于Down综合征,呈X连锁遗传,占X连锁智力低下的40%[1].1992年,Erster SH等报道其发病率男性为1/1250,女性约为1/2000;1997年,De Vries BB等报道其发病率男性为1/4000,女性为1/6000[2-3].发病率下降的原因可能是随着诊断技术的提高,减少误诊的结果.     

甲酰肽受体家族受体与恶性肿瘤

甲酰肽受体家族受体包括甲酰肽受体（FPR）、类甲酰肽受体1和类甲酰肽受体2是主要表达在吞噬细胞表面的一类七次跨膜、G蛋白偶联受体。这些受体被相应的激动剂激活后，能活化吞噬细胞，引起细胞的趋化移动和炎症介质的释放等生物学效应，从而在天然免疫和炎症反应中发挥重要作用。近年来，人们发现甲酰肽受体家族受体也高表达于某些恶性肿瘤细胞的表面，且与这些肿瘤的发生、发展、转移以及治疗有着密切的关系，这无疑揭示了甲酰肽受体家族受体一个新的重要作用，值得深入研究和探讨。