重组人BMP-2骨诱导中神经生长因子的表达

目的通过观察重组人骨形态发生蛋白-2(rhBMP-2)异位诱导成骨过程中神经生长因子(NGF)的表达,探讨NGF在BMP骨诱导中的作用。方法采用ICR小鼠48只,随机分为实验组和对照组,行右侧股后部肌袋异位成骨模型,实验组植入含0．125mg rhBMP-2胶原海绵复合物,对照组仅植入相同体积胶原海绵,分别于术后3、7、14、21d取材,进行放射学、组织学及免疫组化检测。结果放射学和组织学检测均证实,rhBMP-2胶原海绵复合物具有良好的诱导成骨能力;术后第7d,在软骨大量形成期,NGF阳性染色达到高峰．在成纤维细胞、软骨前体细胞、软骨细胞、肥大软骨细胞和成骨细胞中均有阳性表达;术后14、2ld,阳性细胞数量和染色强度有所回落。对照组未见成骨现象。结论首次证实在外源性BMP诱导成骨过程中有明显的NGF表达,提示NGF可能通过直接或间接的方式参与并协同BMP的诱导成骨过程。     

蚕蛹油对糖尿病大鼠血糖和糖代谢相关酶的影响

目的研究蚕蛹油对链脲佐菌素(STZ)诱导的糖尿病大鼠血糖及糖代谢相关酶的影响。方法 SD大鼠以STZ诱导糖尿病模型,大鼠按血糖随机分成6组:模型组,蚕蛹油低、中、高剂量(4.5、6.0、7.5 mL/kg)组,二甲双胍(90 mg/kg)阳性对照组和亚麻籽油(6.0 mL/kg)阳性对照组,另设对照组。各给药组每天ig给药1次,连续4周,对照组和模型组ig生理盐水。每天测大鼠饮食量和饮水量1次,每周末测大鼠体质量和血糖水平,4周后检测大鼠肝脏中己糖激酶(HK)、丙酮酸激酶(PK)以及小肠黏膜中的麦芽糖酶、蔗糖酶、淀粉酶的活性,并进行淀粉耐量检测。结果蚕蛹油各给药组糖尿病大鼠血糖水平呈下降趋势,并呈剂量相关性,HK、PK、麦芽糖酶、蔗糖酶、淀粉酶活性与模型组相比均具有显著差异(P<0.05),且大鼠血糖水平与HK、PK、麦芽糖酶、蔗糖酶、淀粉酶活性的相关性显著(P<0.000 1)。淀粉耐量检测显示,蚕蛹油各给药组大鼠血糖峰值和曲线下面积显著下降(P<0.05)。结论蚕蛹油剂量相关地抑制STZ诱导的大鼠血糖升高,抑制α-葡萄糖苷酶活性,提高HK、PK活性可能是其降血糖作用机制之一。     

医院感染现患率调查分析

目的:了解我院医院感染现患率,探讨现患率调查的优势。方法:对住院患者2003年8月7日零时至24时医院感染现患率进行调查,采用卫生部《医院感染诊断标准》,对所有住院患者医院感染进行床旁调查和病案调查相结合。结果:调查住院患者1124人,发现医院感染45人、49例次,医院感染现患率为4.00%,例次现患率为4.36%;医院感染现患率在0.00%～28.57%之间。下呼吸道感染占40.82%,上呼吸道感染占20.41%,泌尿道18.37%。结论:现患率调查简单、方便,资料更准确。     

细胞周期调控因子p27kip1和DI及SPF值在食管癌检测中的意义

目的:研究细胞周期调控因子p27kip1、DNA指数(DI)和细胞周期S期细胞比值(S-phasefraction,SPF)在食管癌组织中共同检测的意义。方法:对48例食管癌组织采用免疫组织化学方法检测其p27kip1蛋白含量的表达;同时,应用流式细胞术检测DI和SPF。结果:48例癌组织p27kip1蛋白高表达率为33·33%(16/48),而癌周正常组织为78·12%(25/32);癌组织的SPF(1·48±0·32)及DI[(19·48±12·35)%]明显高于癌周组织[1·02±0·17,(9·39±5·75)%],P<0·05;p27kip1低表达组的DI(1·66±0·28)和SPF[(19·78±6·12)%]高于p27kip1高表达组[1·10±0·19,(5·56±5·18)%],P<0·05。并且随着组织学分级和TNM分期的升高,DI和SPF有增加趋势,在Ⅰ、Ⅱ级之间及Ⅰ、ⅡA期之间的差异有统计学意义,P<0·05。结论:p27kip1、SPF和DI共同检测可作为辅助临床判断食管癌的生物学行为及预后的指标。     

口腔正畸模型计算机辅助测量分析系统

利用CCD数字图像获取技术采集牙模的数字图像,基于Windows平台及其Visual C 6.0开发工具,借助于MS SQL Server数据库技术对牙模图像的数据进行维护和管理,研究与开发了口腔正畸模型的计算机辅助测量分析系统.系统设计主要以牙弓形态测量与分析为基础,针对口腔正畸临床诊断及临床操作的需要而进行的.系统包括3个子系统,分别是牙(牙合)模型的病历管理系统、测量系统和分析系统.研究结果表明,口腔正畸模型的计算机辅助测量取代手工测量方式,可提高参数测量分析水平,减少口腔正畸模型的测量误差.     

Apoptosis of human pancreatic carcinoma cells induced by all-trans retinoic acid and interferon

Objective  To investigate the apoptosis of human pancreatic carcinoma PC3 cells induced by the combination of all-trans retinoic acid (ATRA) with interferon alpha (IFN-α). Methods  PC3 cells were treated with ATRA and IFN-α. The inhibitory rate of PC3 cell proliferation was detected using MTT method. Cellular apoptosis was determined with flow cytometry. The percentage of PC3 cell apoptosis was assayed using TUNEL methods. Results  ATRA and IFN-α could inhibit cellular proliferation and induces cellular apoptosis of PC3 cells. The inhibitory effect was stronger when the ATRA and IFN-α were combined as a therapy. Conclusion  ATRA inhibits the proliferation of PC3 cells and induce the apoptosis of PC3 cells. The combination of IFN-α with ATRA may enhance these effects on PC3 cells.      

Inhibitory Effect of Cantharidin on Proliferation of A549 Cells

Objective:To study the inhibition of Cantharidin against the proliferation of human lung cancer A549 cells and its mechanism.Methods:MTT assay was employed to determine the inhibition of Cantharidin against proliferation of A549 cells and flow Cytometry was applied to analyze A549 cell cycle and the effect of Cantharidin on cell cycle.Results:Cantharidin showed inhibition against the proliferation of A549 cells,and the inhibition was mediated by blocking A549 cell cycle at G2/M phase significantly.Conclusion:Cantharidin exhibits inhibition against the proliferation of human lung cancer A549 cells.     

B型利钠肽检测在急诊呼吸困难病因鉴别中应用价值的探讨

目的 探讨B型利钠肽(BNP)在急诊呼吸困难病因鉴别中的应用价值.方法 测定155例急诊呼吸困难患者血浆BNP浓度,并与超声心动图左心室射血分数(LVEF)及E/A值进行相关性分析.以不同BNP浓度为临界值,计算其诊断心源性呼吸困难的敏感性及特异性.结果 心源性呼吸困难组BNP浓度[(764±275)ng/L]与LVEF及E/A值呈明显负相关,且明显高于非心源性呼吸困难组[(113±59)ng/L](P＜0.01).以BNP浓度100、300、500 ng/L为临界值,其诊断心源性呼吸困难的敏感性分别为100.0%、92.2%、80.0%;特异性分别为73.9%、90.8%、100.0%.结论 BNP检测用于急诊呼吸困难病因初步筛查具有重要意义,低水平的BNP临界浓度(＜100 ng/L)有助于排除心源性呼吸困难,高水平的BNP(＞500 ng/L)临界浓度有助于确定心源性呼吸困难,BNP浓度区间100～500 ng/L对于诊断和排除心源性呼吸困难都有一定局限性.

Abstract：

Objective To explore the value of plasma brain natriuretic peptide (BNP)level in differentiating etiology of dyspnea in emergency patients. Methods The plasma BNP levels in 155 emergency patients with dyspnea were assayed. The correlation between BNP levels and left ventricular ejection fraction(LVEF) and E/A respectively. Sensitivity and specificity in differentiating etiology of dyspnea were calculated based on different levels of BNP. Results The BNP levels in cardiogenic dyspnea group were negatively correlated with LVEF and E/A respectively (P ＜ 0.01). They were also significantly higher than those in noncardiogenic dyspnea group (P ＜ 0.01).The sensitivity in diagnosing cardiogenic dyspnea at different critical BNP levels (100 ng/L,300 ng/L,500 ng/L)was 100.0%,92.2%,80.0%,and the specificity was 73.9%,90. 8%,100.0% respectively. Conclusions Plasma BNP level has significant value in preliminarily differentiating etiology of patients with dyspnea. Lower BNP level (＜ 100 ng/L) is helpful in excluding cardiogenic dyspnea and higher BNP level (＞500 ng/L) is helpful in confirming cardiogenic dyspnea. However,BNP level between 100 ～ 500 ng/L isdeficient in confirming and excluding cardiogenic dyspnea.     

毛细管柱气相色谱法同时测定工作场所空气中16种常见有机物

目的建立用毛细管柱气相色谱法同时测定工作场所空气中16种常见有机物。方法采用活性炭管吸附和二硫化碳解吸,氢火焰离子化检测器(FID),经HP-5毛细管柱分离,气相色谱法测定。结果 16种有毒物质的分离效果好,相关系数均在0.9995以上,检测限在0.10～0.75mg/L,相对标准偏差为0.3%～4.3%,回收率为95.2%/～106.1%,解吸效率为77.6%～100%。结论该方法简单、准确、可操作性强,可提高工作效率,适用于实验室的常规监测。     

胃肠道间质瘤预后相关的分子标志物研究进展

胃肠道间质瘤(GISTs)是目前靶向治疗最为成功的实体肿瘤之一,其生物学行为非常复杂,准确判断其良恶性及恶性程度,对于制订正确的治疗方案尤为重要。目前已有研究发现较多具有预后判断价值的分子标志物,综合分析这些标志物的表达情况及其作用网络将有助于GISTs发生发展机制的探索,同时对GISTs的分子分类以及个体化治疗也具有重要的意义。