全文获取类型
收费全文 | 52779篇 |
免费 | 5911篇 |
国内免费 | 4165篇 |
专业分类
耳鼻咽喉 | 446篇 |
儿科学 | 747篇 |
妇产科学 | 597篇 |
基础医学 | 5503篇 |
口腔科学 | 991篇 |
临床医学 | 6955篇 |
内科学 | 7278篇 |
皮肤病学 | 372篇 |
神经病学 | 2507篇 |
特种医学 | 1833篇 |
外国民族医学 | 33篇 |
外科学 | 4890篇 |
综合类 | 10606篇 |
现状与发展 | 18篇 |
一般理论 | 2篇 |
预防医学 | 4184篇 |
眼科学 | 1314篇 |
药学 | 6106篇 |
68篇 | |
中国医学 | 3926篇 |
肿瘤学 | 4479篇 |
出版年
2024年 | 251篇 |
2023年 | 1005篇 |
2022年 | 2464篇 |
2021年 | 3073篇 |
2020年 | 2369篇 |
2019年 | 1963篇 |
2018年 | 1954篇 |
2017年 | 1954篇 |
2016年 | 1773篇 |
2015年 | 2747篇 |
2014年 | 3151篇 |
2013年 | 2958篇 |
2012年 | 4317篇 |
2011年 | 4532篇 |
2010年 | 3141篇 |
2009年 | 2695篇 |
2008年 | 3007篇 |
2007年 | 2774篇 |
2006年 | 2619篇 |
2005年 | 2343篇 |
2004年 | 1681篇 |
2003年 | 1596篇 |
2002年 | 1341篇 |
2001年 | 1139篇 |
2000年 | 1038篇 |
1999年 | 986篇 |
1998年 | 582篇 |
1997年 | 552篇 |
1996年 | 450篇 |
1995年 | 442篇 |
1994年 | 350篇 |
1993年 | 210篇 |
1992年 | 256篇 |
1991年 | 226篇 |
1990年 | 176篇 |
1989年 | 145篇 |
1988年 | 156篇 |
1987年 | 120篇 |
1986年 | 87篇 |
1985年 | 70篇 |
1984年 | 33篇 |
1983年 | 30篇 |
1982年 | 21篇 |
1981年 | 15篇 |
1980年 | 8篇 |
1979年 | 16篇 |
1978年 | 9篇 |
1972年 | 3篇 |
1970年 | 4篇 |
1966年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
目的 评价Peplau人际关系理论对于提高护士沟通能力的影响.方法 对宁波市某三甲医院共50名护士进行了8次的Peplau人际关系理论训练,对训练前后护士的临床沟通能力进行比较.结果 训练后护士的临床沟通能力明显高于训练前,差异有统计学意义.结论 Peplau人际关系理论训练能够有效提升护士的临床沟通能力. 相似文献
992.
Qingying Luo Yanhao Lai Shukun Liu Mei Wu Yuan Liu Zunzhen Zhang 《Environmental and molecular mutagenesis》2012,53(5):325-333
Deregulated expression of DNA polymerase beta (pol β) has been implicated in genomic instability that leads to tumorigenesis, yet the mechanisms underlying the pol β‐mediated genetic instability remain elusive. In this study, we investigated the roles of deregulated expression of pol β in spontaneous and xenobiotic‐induced genetic instability using mouse embryonic fibroblasts (MEFs) that express distinct pol β levels (wild‐type, null, and overexpression) as a model system. Three genetic instability endpoints, DNA strand breaks, chromosome breakage, and gene mutation, were examined under various expression levels of pol β by comet assay, micronuclei test, and hprt mutation assay. Our results demonstrate that neither pol β deficiency nor pol β overexpression is sufficient for accumulation of spontaneous DNA damage that promotes a hyperproliferation phenotype. However, pol β null cells exhibit increased sensitivity to exogenous DNA damaging agents with increased genomic instability compared with pol β wild‐type and overexpression cells. This finding suggests that a pol β deficiency may underlie genomic instability induced by exogenous DNA damaging agents. Interestingly, pol β overexpression cells exhibit less chromosomal or DNA damage, but display a higher hprt mutation frequency upon methyl methanesulfonate exposure compared with the other two cell types. Our results therefore indicate that an excessive amount of pol β may promote genomic instability, presumably through an error‐prone repair response, although it enhances overall BER capacity for induced DNA damage. Environ. Mol. Mutagen. 2012. © 2012Wiley Periodicals, Inc. 相似文献
993.
Jia-Ling Song Yao Yuan Hai-Bo Tan Ri-Ming Huang Hong-Xin Liu Zhi-Fang Xu 《Journal of Asian natural products research》2017,19(3):222-228
Two new coumarins, named (±)-euryacoumarin A (1) and 6-demethylobtusinin (2), and one new natural coumarin, named euryacoumarin B (3), along with two known compounds, scopoletin (4) and obtusinol (5), were isolated from the stems of Eurya chinensis. Their structures were elucidated by means of extensive spectroscopic methods and comparison with data reported in the literatures. Compound 1 exhibited significant inhibition of LPS-induced nitric oxide (NO) production in RAW264.7 cells with IC50 value of 35.64 ± 1.73 μM, and showed marginal antibacterial activities against Bacillus subtilis and B. cereus with MIC values of 50.59 ± 2.12 and 35.42 ± 0.96 μM, respectively. 相似文献
994.
995.
Objective To establish a new procedure for isolating scutellarin from Erigeron multiradiatus. Methods A proposed method was developed by combining macroporous resins with ODS column. Firstly, E. multiradiatus was extracted by ultrasound with 80% methanol. Preliminary separation was performed on macroporous resin column. The performance and adsorption characteristics of three macroporous resins, D140, D141, and D605, were compared and the enrichment procedure was optimized. Further purification was carried out by medium pressure liquid chromatography (MPLC) with ODS column. Results It was demonstrated that D141 had better extractive effects on scutellarin. The MPLC conditions were optimized as follows: 15% ethanol aqueous as mobile phase with flow rate at 2.5 mL/min. The yield and purity of the isolated scutellarin were 1.20 mg/g and 96.5%, respectively. Conclusion The overall procedure is efficient and low-cost, which is considered suitable for the separation and purification of scutellarin from E. multiradiatus. The results provide the scientific basis for developing and using scutellarin in clinic. 相似文献
996.
背景:移植肾输尿管梗阻是肾移植后最常见的并发症,传统上需采用开放手术修复,但腔内泌尿外科技术的发展和经验的积累已使内镜技术成为临床处理这类并发症的另一选择。目的:回顾性分析腔内泌尿外科技术处理移植肾输尿管梗阻的临床效果。方法:2001-02/2010-10对23例移植肾输尿管梗阻患者采用腔内技术治疗。术中采用气囊扩张或腔内切开梗阻后,留置两条双J管4~6周。术后定期随诊,行B超、肾图和肾功能检查。结果与结论:4例患者采用气囊扩张,19例采用腔内切开。术中均成功将梗阻段扩张或切开,术中和术后无并发症发生。随访6~108个月,14例输尿管引流通畅,肾功能稳定;9例梗阻复发,其中2例采用长期输尿管换管,1例永久肾造瘘,6例患者改开放手术治疗(4例成功,2例失败后采用长期输尿管换管)。提示腔内泌尿外科技术处理移植肾输尿管膀胱吻合口梗阻安全、有效,但首次内切开失败后再次腔内治疗的复发率高。 相似文献
997.
998.
999.
Xianglong Liu Xiangfei Yuan Hai Qin Xipeng Zhang 《APMIS : acta pathologica, microbiologica, et immunologica Scandinavica》2017,125(2):93-100
Rectal cancer is a common malignant tumor of the digestive tract, with a high incidence and high mortality. This study aimed to identify the potential biomarkers and therapeutic targets for rectal adenocarcinoma (RAC) metastasis. The expression profiling of RAC patients with metastasis and RAC patients without metastasis was downloaded from The Cancer Genome Atlas (TCGA) database. The datasets were used to identify the genes associated with RAC metastasis. Fifty up‐regulated genes and seventeen down‐regulated genes were identified in the primary tumor loci of RAC metastasis compared with non‐metastasis. Sixty‐seven dysregulated gens were conducted to construct the protein–protein network, and CCND3 was the hub protein. The dysregulated genes were significantly enriched in pancreatic secretion, cell adhesion molecules pathways, response to vitamin D of biological process, and retinoid binding of molecular function. Quantitative real‐time polymerase chain reaction results demonstrated that CCND3, AQP3, PEG10, and RAB27B had the up‐regulated tendency in RAC metastasis; ADCY1 had the down‐regulated tendency in RAC metastasis. CCND3, AQP3, PEG10, RAB27B, and ADCY1 might play essential roles in the metastasis process of RAC through pancreatic secretion and cell adhesion molecules pathways. The five genes could be potential diagnosis biomarkers or therapeutic targets for RAC metastasis. 相似文献
1000.