Increased expression of receptor activator of nuclear factor-κB ligand in osteoblasts from adolescent idiopathic scoliosis patients with low bone mineral density

Persistent generalized low bone mineral density (BMD) has been reported in patients with adolescent idiopathic scoliosis (AIS).However,the exact mechanisms and causes of the low BMD in AIS patients are largely unknown.The purpose of this study was to examine the relationship between the receptor activator of NF-κB ligand (RANKL)/osteoprotegerin (OPG) levels in osteoblasts (OBs) from AIS patients with low BMD and with comparison made between the patients and controls.Twenty AIS patients and eight age-matched controls were included in the present study.The BMD of lumbar spine and proximal femur was measured in all subjects.OBs from the cancellous bone of each subject was harvested and primarily cultured.The mRNA and protein expression of RANKL and OPG in OBs was detected by RT-PCR and Western blotting.The results showed BMD was lower in AIS patients than in controls.A significantly higher mRNA and protein expression of RANKL was observed in OBs from AIS patients,while no significant difference was found in the expression of OPG between AIS patients and controls.As a result,RANKL/OPG ratio in patients with AIS was remarkably higher than controls.Our study preliminarily demonstrated expression of RANKL was higher in OBs from AIS patients with low BMD as compared with controls,suggesting the unbalanced RANKL/OPG ratio caused by an over-expression of RANKL in OBs may be responsible for the low BMD in AIS patients.     

应急救援专业技术网络教学平台应用研究

通过电子化学习技术(E-learning),将现有的网络资源和应急救援数字化教学培训相结合,充分利用现代信息技术所提供的全新沟通机制与丰富资源,为应急救援培训提供一个受众面更为广阔的平台,进一步为培训基地开展应急救援培训提供科学的、互动的、个性化的和不受时空约束的教学管理平台.该平台有利于各级人员根据自我需求制定各自的...     

胃癌组织中人表皮生长因子含量和临床意义

检测人表皮生长因子（ｈＥＧＦ）在胃癌组织中的含量，初步探讨其临床意义。用放射免疫分析法测定５２例胃癌切除标本和１８例正常胃粘膜组织中ｈＥＧＦ的含量。结果：对照组为１．４９±０．９５ｎｇ／ｇ湿重，胃癌组织为４．２９±２．３５ｎｇ／ｇ湿重，差异显著（Ｐ＜０．０１）。在胃癌组织中，ｈＥＧＦ含量进展期高于早期，弥漫型高于肠型，淋巴结转移阳性者高于阴性者，近期随访发现胃癌患者死亡组的ｈＥＧＦ含量较高。结论：胃癌组织可产生过量的ｈＥＧＦ，并与肿瘤浸润、组织分化程度、淋巴结转移等相关，提示胃癌组织ｈＥＧＦ测定可作为判断胃癌生物学行为及了解患者预后的指标。     

血清白细胞介素-13及肺功能检测与儿童毛细支气管炎的相关性研究

目的：探讨血清白细胞介素-13（IL-13）与肺功能检测在儿童毛细支气管炎的临床意义。方法：采用双抗体夹心酶联免疫吸附试验（ELISA）对15例毛细支气管炎急性期、10例恢复期患儿外周血行血清IL-13水平测定;并与10例健康对照组儿童比较。结果：毛细支气管炎恢复期组血清IL-13水平较急性期组明显下降（P〈0.01）,但两组均明显高于对照组（P均〈0.01）;毛细支气管炎恢复期组肺功能指标中呼出75%潮气量时的呼气流速/潮气呼气峰流速（25/PF）及潮气呼气中期流速/潮气吸气中期流速（ME/MI）均较急性期组明显升高（P均〈0.01）,但25/PF仍低于对照组（P〈0.01）,而ME/MI与对照组比较无显著性差异（P〉0.05）。毛细支气管炎急性期组血清IL-13水平与25/PF呈显著负相关（r=0.38,P〈0.05）,与ME/MI无相关性（r=-0.002,P〉0.05）。结论：IL-13参与毛细支气管炎发病过程,降低其水平有助于毛细支气管炎的痊愈;毛细支气管炎患儿急性期肺功能主要表现为小呼吸道阻塞性通气功能障碍。     

公共关系课程对大学生公关意识与能力影响的研究

目的:通过<公共关系学>课程的教学,提高大学生的公关意识和能力.方法:在3所大学采用理论教学、课堂讨论、无记名问卷调查等方式开展教学.结果:405名学生的问卷调查显示:选择<公共关系学>教学能"逐步树立公关意识、形象意识"者占95.1%(385/405);能"提高学生综合素质"者占93.1%(377/405);能"提高学生的观察反应能力"者占82.5%(334/405);能"提高学生的自控应变能力"者占78.3%(317/405).结论:公关理念能增强公关意识,主动塑造自身良好社会形象;能培养公关技能,提高大学生的社会适应能力.     

SHP-1 acts as a tumor suppressor by interacting with EGFR and predicts the prognosis of human breast cancer

Objective:The aims of this study were to examine the prognostic value of SHP-1 in breast cancer, its roles in the regulation of breast cancer cell growth and metastasis, and the underlying mechanisms.Methods:Tumor specimens from 160 patients with breast cancer and 160 noncancerous tissues were used to examine the expression of SHP-1 and to analyze its association with overall survival through Kaplan–Meier and multivariate Cox regression analyses. RNA sequencing data and the expression and clinical importance of SHP-1 in breast cancer were evaluated with data from The Cancer Genome Atlas. In vitro and in vivo assays were performed to elucidate the effects of SHP-1 on breast cancer cell proliferation and invasion. Confocal immunofluorescence and GST pulldown assays were used to demonstrate the interaction between SHP-1 and epidermal growth factor receptor, as well as its downstream pathways. Immunohistochemistry and The Cancer Genome Atlas database were used to investigate the clinical association between SHP-1 and EGFR in human breast cancer.Results:SHP-1 expression was associated with better survival in patients with breast cancer, whereas SHP-1 expression was negatively correlated with EGFR in human breast cancer. Ectopic SHP-1 expression significantly suppressed breast cancer cell proliferation, migration, and invasion. SHP-1 knockdown induced a more invasive phenotype and accelerated cell growth. Mechanistically, EGFR, a protein directly interacting with SHP-1, mediates the SHP-1-induced inactivation of Ras/Erk/GSK3β signaling and its downstream effectors.Conclusions:SHP-1 is an important prognostic biomarker in patients with breast cancer, and the SHP-1-EGFR axis is a promising target for treatment.     

快速康复外科护理新路径在痔疮患者围手术期的应用研究

目的：探讨快速康复外科护理新路径在痔疮患者围手术期的应用。方法：将2013年－2014年我科室收治的200例痔疮手术患者随机分为观察组与对照组,每组各100例患者,对观察组痔疮患者采用快速康复护理新路径进行护理,对照组采用传统常规护理方案进行护理,对两组护理方式的康复结果进行对比。结果：两组患者在愈合时间、首次排便时间、健康教育知晓率、患者满意度、术后并发症发生率等方面的差异具有统计学意义（ P ＜0．05）。结论：快速康复外科护理新路径在痔疮患者围手术期的应用可缩短伤口愈合时间、减少术后并发症,有利于患者康复,具有良好的社会效益。     

浅祈中职生做人教育

随着现代社会对人才综合素质要求的提高,做人教育越来越受到人们的重视.做人教育就是使学生学会做人,学会做一个有良好的个性品质和道德修养、有较强的事业心和责任感并能够与他人和谐相处、有益于社会和谐发展的人.     

Elevated Glycated Albumin in Serum Is Associated with Adverse Cardiac Outcomes in Patients with Acute Coronary Syndrome Who Underwent Revascularization Therapy

Aims: The associations between increased glycated albumin (GA) in the serum and diabetic complications and mortality have been revealed in the general population. However, less is known regarding the prognostic value of GA in patients diagnosed with acute coronary syndrome (ACS). Methods: In this study, all patients admitted for ACS who underwent a successful percutaneous coronary intervention (PCI) at our center from January 2018 to February 2019 were retrospectively examined. Clinical characteristics, laboratory results (e.g., serum GA levels), and procedural details were collected. The primary outcome included a composite of major adverse cardio-cerebral events (MACCE), such as death, myocardial infarction, stroke, and unplanned revascularization. The association between serum GA levels and clinical outcomes was tested in three multivariable models using Cox proportional hazard analysis. Subgroup analysis was performed in patients who were diagnosed with diabetes versus patients without diabetes. Results: A total of 1,806 ACS patients (mean age of 59.4 years; 77.8% were men; 44.9% were diagnosed with diabetes) were enrolled in this study, where the majority exhibited unstable angina (81.6%) and showed preserved left ventricular systolic function. Patients in the high GA level group were commonly female and were more likely to have metabolic disorders and to exhibit severe CAD (all p ＜0.05). MACCE occurred in 126 patients (7.0%) during a mean follow-up time of 17.2 months. The cumulative risk of MACCE at the 18-month follow-up visit significantly increased in a stepwise fashion along with increased GA levels (log-rank p =0.018) in the serum. The association between serum GA levels and MACCE was further determined after adjusting traditional risk factors and hemoglobin A1c (HbA1c) (GA, per 1% increase: hazard ratio [HR] 1.09, 95% confidence interval [CI] 1.06–1.13; GA, higher vs. lower tertial: HR 1.92, 95% CI 1.01–3.67). In a subgroup analysis, the prognostic role of serum GA only existed in diabetic patients, even when adjusting for traditional risk factors and HbA1c levels. Conclusions: Elevated GA levels in the serum were associated with poor intermediate-term outcomes in low-risk ACS patients who underwent PCI, especially in patients with preexisting diabetes.