MTX和米非司酮联合治疗异位妊娠的疗效观察

目的:观察MTX联合米非司酮治疗异住妊娠的疗效。方法:将本院2006年1月~2007年11月间收治的54例异位妊娠患者随机分为对照组26例和观察组28例。对照组给予一次性肌肉注射MTX治疗,观察组在给予一次性肌肉注射MTX治疗基础上,同时加服米非司酮,共服3d,比较两组患者治疗疗效。结果:观察组治疗成功率较对照组明显提高,用药后血B-HCG、盆腔包块改变显著,两组比较差异显著P<0.05。两组不良反应发生率比较差异不明显P>0.05,经对症处理后均明显好转。结论:MTX联合米非司酮治疗异位妊娠可提高成功率,且不加重不良反应,明显优于单纯使用甲氨蝶呤。     

人工流产术后闭经的原因分析

目的分析人工流产术后发生闭经的原因,为相关的临床实践提供参考。方法对82例人工流产术后发生闭经的患者进行宫腔镜检查、病理检查和激素测定。结果宫腔内口粘连9例,宫腔粘连25例,宫腔瘢痕13例,宫内残留物8例,宫腔内未见异常27例;82例均刮出内膜组织,其中属于增生期内膜的45例,分泌期内膜的22例,内膜增生过长的15例;有排卵的9例,无排卵的16例,卵巢功能早衰9例,高催乳素血症13例,丘脑功能失调性闭经15例,20例FSH、LH均低下。结论宫腔异常和下丘脑-垂体-卵巢轴系统功能失调是女性人工流产术后闭经的原因。     

记忆合金环抱器用于肋骨骨折内固定的手术配合

目的 总结镍钛形状记忆合金环抱器用于肋骨骨折内固定手术的配合要点.方法 对31例肋骨骨折实施镍钛形状记忆合金环抱器内固定手术,术前了解患者生命体征、血氧饱和度(SpO2)、肋骨骨折的根数及有无其他并存疾病,准备镍钛形状记忆合金环抱器及0～4℃无菌生理盐水;术中密切观察患者的生命体征变化,及时提供0～4℃生理盐水和型号合适的镍钛形状记忆合金环抱器;术后妥善固定胸腔引流管,密切观察SpO2.结果 31例手术均顺利完成,无1例出现手术并发症.结论 手术室护士对相关手术知识的掌握以及完善的术前准备和及时的术中配合是手术成功的保证.     

Altered P-selectin and CD44 expression in the renal tissues and peripheral blood of children with IgA nephropathy

Objective  To understand the role of P-selectin (CD62P) and CD44 in mediating immune inflammation in the nephrotic process of children with IgA nephropathy (IgAN), cooperative expression of CD62P and CD44 in peripheral blood and renal tissues of IgAN children was investigated and its association with changes of histopathologic, serologic, and urinary properties was tested. Material and methods  Forty-six IgAN children were divided into three groups according to pathologic grades and clinical features. Fifteen blood samples from normal children and four normal renal biopsy specimens were used as controls. Plasma level of CD62P was detected by double antibody sandwich immunoradiometric assay; ELISA was used to determine serum level of CD44. Expression of CD62P and CD44 in renal tissues was determined by immunohistochemistry. Results  Cooperative expression of CD62P and CD44 was detected in renal tissues and peripheral blood of IgAN children. Altered expression of CD62P and CD44 in peripheral blood significantly correlated not only with hematuria, proteinuria, serum cholesterol, and albumin, and with urine NAG and β₂-MG, but also with degree of tubulointerstitial injury in IgAN children. Conclusion  The evidence supported CD62P and CD44 as initial and promoting factors mediating immune inflammation in the nephrotic process in IgAN children. The cooperative expression profiles of CD62P and CD44 in renal tissues and peripheral blood combined with serologic and urinary predictors may be important in diagnosis of progression in children with IgAN.     

Analyses of Bcl-2, Survivin, and CD44v6 expressions and human papillomavirus infection in cervical carcinomas

Infection with human papillomaviruses (HPV), and suppression of apoptosis and cell adhesion are putative aetiological factors to cervical carcinogenesis. However, controversial results have been reported with respect to their relationships with cervical carcinomas. Here we analysed papillomavirus infection, apoptotic index (AI), expressions of the anti-apoptotic proteins Bcl-2 and Survivin, and expression of the cell-adhesion protein CD44 in cervical tissue samples from individuals with and without cervical carcinomas. Although both HPV16 and HPV18 are reportedly important aetiological factors, we found that cervical carcinomas were highly associated with HPV16 but not HPV18 infection. Immunohistochemistry showed that the percentages of cells expressing Bcl-2, Survivin, and CD44v6 were greatly increased in samples of cervical carcinomas. Furthermore, the expression rates of Survivin and CD44v6 increased whereas that of Bcl-2 declined as cervical cancers developed into more advanced clinical or histopathological stages. Surprisingly, there was little difference in AI between control and cervical cancer samples. These observations provide further evidence that HPV infection, apoptosis and cell adhesion abnormalities are related to cervical cancers. They also suggest that Bcl-2, Survivin and CD44v6 expressions, and HPV16 infection could be useful indices in screening of cervical carcinomas.     

卵巢癌细胞IL-6、IL-8分泌与其化疗敏感性及耐药相关基因和凋亡抑制基因表达关系的初步探讨

目的 分析比较4种常见的人E皮性卵巢癌细胞系IL-6、IL-8分泌与其化疗敏感性及部分耐药相关基因和凋亡抑制基因表达的关系,为今后研究IL-6、IL-8诱导卵巢癌细胞对化疗药物产生耐药的机制奠定基础.方法 IL-6、IL-8及其受体的表达采用RT-PCR、ELISA及免疫印迹技术进行检测,卵巢癌细胞对顺铂和紫杉醇的敏感性采用M1Tr试验进行分析,耐药相关基因和凋亡抑制基因的表达则采用RT-PCR进行测定.结果 1)4种卵巢癌细胞除A2780细胞外均组成性分泌IL-6和IL-8,二者转录水平与其蛋白水平基本一致.2)4种卵巢癌细胞均表达IL-6受体和IL-8受体.3)4种卵巢癌细胞对顺铂和紫杉醇的敏感性不同,其中A2780细胞最敏感,ES-2细胞次之,CAOV-3和SKOV-3细胞则有不同程度的耐药性.4)部分耐药相关基因和凋亡抑制基因在A2780和ES-2细胞中的表达水平均较低,而在CAOV-3和SKOV-3细胞中的表达水平均较高.结论 卵巢癌细胞IL-6、IL-8的自分泌水平(尤其是IL-6的水平)与其对顺铂和紫杉醇的敏感性基本呈负相关趋势,与其部分耐药相关基因和凋亡抑制基因的表达水平相一致.     

HPV L1 C-末端保守序列短肽体液免疫学特性

目的 探讨一段位于HPV L1 C-末端、长30个氨基酸残基的保守序列的体液免疫学性质而进行此实验.方法 以此序列为基础人工合成短肽,以该短肽免疫小鼠和兔,获得抗血清,通过ELISA,Western Blot及免疫组织化学的方法,分别对含HPV6b,16及18 L1的细胞裂解物、重组蛋白或HPV阳性临床标本进行反应.结果 抗短肽抗血清能与含HPV6b,16及18 L1的细胞裂解物、重组蛋白发生针对HPV L1的反应,能使HPV6和16阳性的临床标本呈现阳性反应,而抗HPV16及抗重组HPV16 L1抗血清对此短肽的反应则较弱.结论结果表明由该保守序列短肽诱导的抗血清具有一定的型间交叉反应特征,这一特性可能对研究检测用广谱HPV L1抗体有一定的意义.该序列短肽抗血清对不同型HPV L1反应的差异性及抗血清对多型别HPV L1的反应是否具有中和性,尚需进一步的研究.     

针刺心包经内关、郄门对急性轻度缺氧健康志愿者心输出量的影响

目的 现察针刺心包经穴位时急性轻度缺氧健康志愿者心输出量的影响. 方法在吸入低氧混合气体条件下,电针心包经穴位,用多道生理信号采集系统记录心输出量(CO)、搏出量(SV)、心率(HR)3项指标在针刺前后的变化. 结果电针内关穴能显著降低CO和SV,电针郄门能明显降低CO. 结论电针内关、郄门能降低健康志愿者急性轻度缺氧...     

milk-145通过下调OCT4基因仰制肺腺癌干细胞增殖

背景与目的 miR-145是通过miRNA芯片及qPCR验证筛选出的一种潜在肺癌保护性miRNA。本研究旨在探讨miR-145与肺癌干细胞之间的关系及分子机制。方法 miRNA芯片对肺腺癌患者瘤旁和正常组织进行表达谱分析;生物信息学软件预测miR-145潜在的靶基因;脂质体2000介导转染miR-145模拟物和阻遏物进入A549细胞株;实时定量PCR检测miR-145表达水平;Western blot检测OCT4蛋白水平;双荧光素酶报告基因验证miR-145是否作用于OCT4mRNA的3'UTR区预测靶位;细胞增殖实验检测miR-145对于A549细胞生长的作用;流式细胞术检测干细胞表型CD133+的表达。结果在肺腺癌组织中miR-145表达明显低于瘤旁正常组织;miRanda软件预测OCT4是miR-145潜在靶基因;与对照组相比,miR-145模拟物组和阻遏物组miR-145表达分别明显上调和下调;miR-145对A549细胞的生长有双向调节作用,过表达miR-145抑制细胞生长;过表达miR-145可明显降低OCT4蛋白水平及干细胞表型CD133百分比,而抑制miR-145表达则明显增加OCT4蛋白水平...     

扎鲁司特的合成工艺改进

目的合成扎鲁司特,改进工艺。方法5-硝基-1H-吲哚经甲基化、付-克反应、缩合、还原和酰胺化反应制得扎鲁司特,主要对甲基化、付-克反应和还原工艺进行了改进。结果通过优化条件合成了扎鲁司特,总收率31．6％,目标化合物结构经红外、质谱、核磁共振谱、元素分析确证。结论改进后的工艺原材料价廉易得,降低了生产成本,更适合工业化生产。