颞下颌关节的三维有限元法研究

本研究利用颞下颌关节ＣＴ扫描资料和计算机图像分析处理技术以及三维有限元方法相结合建立了髁状突的三维有限元模型，结果表明，此方法在技术上是可行的并将有助于颞下颌关节的临床和基础研究。     

不同来源HSP70的氨基酸组成分析

使用ＤＥＡＥ－５２离子交换层析和ＡＴＰ琼脂糖亲和层析方法，从人体肿瘤细胞株，大鼠肝脏和心脏、家兔和小鼠肝脏组织中纯化了主要的热应激蛋白－ＨＳＰ７０，用氨基酸自动分析仪分析它的氨基酸组成。     

血液白细胞图象的计算机自动分割方法研究

本文提出了一套血液白细胞图象的计算机自动分割方法。实际测试表明：该方法稳定可靠且有效。单个白细胞检出成功率达９６％，区域分割吻合率平均达９４％，现已将该方法装入作者研制的白细胞自动分类识别ＬＥＵＫ分析系统的分割模块中。     

真皮下血管网皮瓣修复感染创面的研究

目的：探讨真皮下血管网皮瓣的抗菌能力和临床修复感染创面的应用。方法：以家猪为实验动物，在臀部两侧分别形成传统的岛状皮瓣和真皮下血管网岛状皮瓣，用细菌计数、吖啶橙荧光染色测定白细胞吞噬指数和白细胞内杀菌率等方法，进行对比。结果：真皮下血管网岛状皮瓣的抗菌力明显降低。 １９８９年起临床应用 ５例，创面彻底清创后用吻合血管的真皮下血管网游离皮瓣修复，全部成活。结论：该皮瓣抗菌力虽低于传统皮瓣，但仍可用于修复感染创面。     

VACUOLE SIGN AND SMALL NODE SIGN IN EARLY PERIPHERAL LUNG CANCER PATHOLOGIC BASIS AND DIAGNOSTIC VALUE

Radiologic and pathologic mani;estations of 30 peripheral pulmonary masses around l.5-3 cm in dia- meter were compared to the pathologic basis of va- cuole sign and small node sign on tomogram. Stati stics of 100 conventional X-ray tomograms of peri- pheral pulmonary masses about l-3 cm in diameter suggested that the vacuole sign and small node sign, more common in early peripheral lung cancer than in tuberculoma, are valuable for differentiating early peripheral lung cancer from tuberculoma.     

安徽省回族居民中两例Hb D Punjab纯合子一级结构分析

继青海、甘肃、新疆、河南等地发现Ｈｂ Ｐｕｎｊａｂ杂合子后，杂们又在安徽省阜阳地区发现两例ＨｂＤ纯合子，级一级结构分析，确诊为ＨｂＤｐｕｎｊａｂβ１２１（ＣＨ４）Ｃｌｕ→Ｇｌｎ。这一发现为探讨该地区回族中异常Ｈｂ发生率高的原因，研究各民族的亲缘关系以及Ｈｂ变对人类健康与的影响提供了遗传学资料。     

实验冻伤大鼠神经纤维的超微结构改变

为探讨冷冻损伤发生、发展及转归的规律，采用健康Ｗｉｓｔａｒ大鼠，分为冻伤组（２０只）和对照组（５只）。电镜下观察大鼠后肢冷冻至－２０℃后即刻、４小时、２４小时及７２小时时周围神经的超微结构改变。冻后即刻，神经髓鞘即出现轻度变性；冻后１～２４小时，有髓神经雪旺细胞及轴突严重变性，而无髓神经变性较轻；冻后７２小时，神经溃变及修复并存，无髓神经较有髓神经易于修复。     

恶性疟P190抗原保守区Ⅰ、Ⅴ基因的亚克隆及在大肠杆菌中高效表达

作者采用PCR方法克隆了我国海南省FCC1/HN株P190抗原两个保守区基因,分别定名为P190CRI和P190CRV。基因片段经纯化后连接到pUC18载体中进行DNA序列分析,结果显示:除了P190CRV中有5个碱基变换外,其余序列均与MAD20型序列一致。经序列分析的两个基因片段分别与pGEX-2T载体连接,经双酶切鉴定后转化感受态JM109(DE_3)大肠杆菌进行高效融合表达,并且用Sepharose 4B-谷胱甘肽层析柱进行亲和纯化,结果为:两个插入基因片段均得到高效融合表达,经一步亲和纯化后就取得高纯度的重组蛋白。     

Cloning of Human α-defensin-1 (HNP-1) Gene and Construction of Its Eukaryotic Expression Vector

1 Introduction Defensins are small cationic antimicrobial peptides that function in the host's innate immune system. The human defensin family includes three small peptides from the azurophil granules of polymorphonuclear cells named human neutrophil peptide (HNP)-1, HNP-2, HNP-3,which consist 5%-7% of the protein of human neutrophil. HNP-4 is approximately one hundred times less abundant. They demonstrate antibacterial, antifungal and antiviral properties in vitro. HNPs are important component of nonoxidative mechanism in macrophages, and can direct inactivate the enveloped viruses. Because only special cells express defensins. And it is hard to extract them naturally and the production is few. So researcher expect to obtain defensins highly through heterogenous expression by gene engineering technology. In order to express HNP-1, we cloned the cDNA of HNP-1 from human polymorphonuclear cells in peripheral blood, and constructed its eukaryotic expression vector, which provided a base for the further study on its mechanism of antimicrobial effect.