鹿茸多肽干预膝骨性关节炎软骨细胞的增殖

背景:软骨细胞体外培养实验证实,鹿茸多肽其具有显著的促细胞有丝分裂活性,可刺激软骨细胞的增殖。目的:观察鹿茸多肽对实验性骨性关节炎相关细胞因子的调节作用和对软骨细胞增殖的影响。方法:将新西兰大白兔随机分成正常组,假手术组和模型组。正常组不予任何处理,假手术组左膝关节内侧皮肤切开后缝合,模型组建立左膝骨性关节炎模型。模型组建模成功后再将随机分为2组,鹿茸多肽组给予鹿茸多肽针剂生理盐水稀释液关节腔注射干预,生理盐水组给予生理盐水关节腔注射作为对照,干预后第1,7,15,30天分别观察鹿茸多肽组和生理盐水组关节软骨形态学变化和软骨细胞结构变化;酶联免疫吸附法检测关节液中白细胞介素1β,肿瘤坏死因子α和转化生长因子β1的水平,免疫组织化学法检测关节软骨增殖细胞核抗原表达并计算细胞增殖指数。结果与结论:在相同时间段内,与生理盐水组相比,鹿茸多肽组关节软骨增殖细胞核抗原表达,细胞增殖指数及关节液中转化生长因子β1含量均增高(P<0.05),关节液中白细胞介素1β和肿瘤坏死因子α水平明显降低(P<0.05)。结果证实鹿茸多肽可降低实验性骨性关节炎过程中白细胞介素1β和肿瘤坏死因子α水平,提高转化生长因子β1水平,并可促进关节软骨细胞的增殖。     

Additive antitumoral effect of interleukin-12 gene therapy and chemotherapy in the treatment of urothelial bladder cancer in vitro and in vivo

Background  

We evaluated antitumoral effect of combined chemotherapy and interleukin-12 (IL-12) gene therapy in in vitro and in vivo experimental urothelial bladder cancer (UBC) model.     

Preauricular transcondylar approach for basal cell adenoma of parotid coexist with ganglion cyst of the ipsilateral temporomandibular joint

The concurrence of 2 independent neoplasias in the ipsilateral parotid and the temporomandibular joint (TMJ) region was infrequently reported. In this article, we present a unique case characterized by the coexistence of a rare salivary gland tumor, basal cell adenoma, of the parotid gland with a ganglion cyst in the ipsilateral TMJ region. A special surgical procedure was also presented here using a modified preauricular incision and transcondylar approach for extracapsular dissection of both lesions. Previously published literature are about the traditional treatment of benign parotid tumors using partial or total parotidectomy with the preservation of facial nerve mainly via an S-shaped submandibular incision and approach. Here, for the first time, a special surgical method using preauricular transcondylar approach for this unique case of synchronous occurrence of parotid tumor and TMJ cyst is reported.     

Flash visual evoked potentials at 2-year-old infants with different birth weights

Background  

Increased prevalence of visual impairments has been reported in preterm populations. However, it remains unclear about the long-term visual electrophysiological outcomes and their association with visual cognitive functions in premature infants. We investigated visual electrophysiological outcome of 2-year-old infants of different birth weights by flash visual evoked potentials (FVEPs) in order to explore the correlation between visual cognitive functions and FVEPs and to assess the application of FVEPs in evaluating the visual capability of an infant.     

连续血液净化治疗在危重症患儿中的应用

目的 探讨连续血液净化治疗(CBP)在PICU危重患儿中的应用价值.方法 实施CBP治疗患儿18例,其中急性肾功能衰竭14例,行连续静脉-静脉血液滤过(CVVH)治疗;另外2例瑞氏综合征、2例吉兰-巴雷综合征患儿行血浆置换(PE)治疗.观察治疗前后患儿临床症状、血生化、血气指标及氧合的变化情况.结果 14例急性肾功能衰竭患儿CVVH治疗后血清尿素氮(BUN)、肌酐(Scr)明显下降[(48.6±14.8)mmol/L vs(28.9±5.4)mmol/L;(634.3±258.2)μmol/L vs(318.4±143.5)μmol/L],血钾、pH值基本维持正常范围,氧合也有明显的改善.14例患儿中12例肾功能恢复正常.2例吉兰-巴雷综合征患儿经PE治疗后呼吸困难、肌力明显改善,顺利撤离呼吸机.2例瑞氏综合征患儿PE治疗后ALT、AST、血氨明显下降,经包括PE在内的综合抢救治疗后好转出院.CBP治疗并发症:穿刺口渗血3例,体温不升2例.治疗过程中患儿生命体征稳定,未见血压、脉搏异常波动.结论 CBP对急性肝、肾功能衰竭及自身免疫性疾病危重患儿的治疗安全有效.     

Serum C-reactive protein and soluble angiopoietin receptor Tie-2 in patients with acute myocardial infarction and its detection by optical proteinchip

Serum C-reactive protein (CRP) and soluble angiopoietin receptor Tie-2 (sTie) were detected in patients with acute myocardial infarction (AMI). The results indicated that mean serum CRP and Tie-2 levels were significantly higher in the patients with AMI than the control group. Increasing CRP was related to an increased infarction area and adverse prognosis. Levels of sTie-2 increased in the AMI patients and the maximum level of sTie-2 appeared at day 2 after onset of AMI. The feasibility of using to detect serum CRP and Tie-2 was also presented in this study. Measurement for CRP by optical proteinchip with imaging ellipsometry (OPC-IE) and immunobidimetric analyzer showed no obvious difference (p<0.01). Also, the measurement for Tie-2 by ELISA and OPC-IE showed no obvious difference (p<0.01).     

Effect and mechanism of melatonin's action on the proliferation of human umbilical vein endothelial cells

Melatonin is the major secretory product of the pineal gland and is considered an important natural oncostatic agent. The anticancer activity of melatonin is due to its immunomodulatory, anti-proliferative and antioxidative effects. At present there are no direct data available as to melatonin's possible influence on angiogenesis, which is a major biological mechanism responsible for tumor growth and dissemination. The current study investigated the influence of melatonin on angiogenesis. Human umbilical vein endothelial cells (HUVECs) were cultured, identified, and purified. Cell growth and viability, DNA fragmentation and cell cycle analyses were determined. To elucidate the mechanism of action of melatonin, Western blot analyses for P53, Bax and Bcl-2 expression were carried out. The results demonstrate the anti-proliferative and apoptosis-inducing effects of melatonin; these changes were associated with cell cycle arrest, upregulation of P53 and Bax and downregulation of Bcl-2. Taken together, our data showed that melatonin in high concentrations markedly reduces HUVECs proliferation, induces cellular apoptosis, and modulates cell cycle length. P53 and Bax/Bcl-2 expression changes may be involved in these actions of melatonin.     

胃癌组织中MMP-9和TIMP-1的表达及其临床意义

背景：有关胃癌组织中基质金属蛋白酶（MMPs）和金属蛋白酶组织抑制剂（TIMPs）表达的研究不多且结果不一。目的：探讨胃癌组织中MMP-9和TIMP-1的表达及其临床意义。方法：收集临床病理资料完整的98例胃癌患者。应用免疫组化法检测组织中MMP-9和TIMP-1表达．并分析其与临床病理特征的关系以及各参数对胃癌患者预后的影响。结果：胃癌组织中MMP-9高表达与浸润深度、淋巴结转移和TNM分期显著相关（P〈0．01）。单因素分析显示胃癌患者预后与MMP-9高表达（P=0．014）、浸润深度（P〈0．001）、淋巴结转移（P〈0．0001）和TNM分期（P〈0．0001）相关。TIMP-1表达与胃癌患者临床病理特征和预后无关。结论：MMP-9高表达与胃癌的发生、进展有关,可作为胃癌患者预后指标之一。