肿瘤科护士共享决策知信行现状及影响因素调查

目的 调查肿瘤科护士共享决策知识、态度、行为现状并分析其影响因素,为开展共享决策培训提供支持.方法 采用自制的肿瘤科护士共享决策知信行调查问卷对郑州市三级甲等综合医院和肿瘤专科医院的351名肿瘤科护士进行横断面调查.结果 肿瘤科护士共享决策知识、态度、行为得分分别为(34.76±6.99)分、(21.48±4.00)分...     

上腔静脉塌陷指数预测机械通气患者容量反应的可行性

目的 探索上腔静脉塌陷指数(SVC?CI)对机械通气患者容量反应的预测效果.方法 选择择期气管插管全麻机械通气下行腹部手术患者36例,男20例,女16例,年龄18～64岁,ASAⅠ或Ⅱ级,NYHAⅠ或Ⅱ级.所有患者在全麻诱导后至手术开始前,快速输注复方乳酸钠10 ml/kg,输注时间10～15 min.记录补液前后MA...     

预产期前音乐干预缓解产妇分娩疼痛

目的探讨音乐无痛分娩法对缓解产妇分娩过程中疼痛的效果。方法将在我院产科行围产期保健并分娩的孕产妇140例随机分为观察组和对照组各70例。对照组接受常规围产保健及产程护理;观察组在此基础上,于预产期的前8周开始应用音乐干预疗法。包括采用音乐处方,引导产妇将注意力聚集在音乐上,以及在临产后自带音乐CD或MP3进入待产室,为其播放熟悉音乐。对两组产程各个阶段的疼痛程度、分娩方式及产程时间进行比较。结果两组产程不同阶段(除第三产程)疼痛评分比较,差异有统计学意义(P0.05,P0.01);观察组第一产程、第二产程及总产程时间较对照组显著缩短,剖宫产率显著降低(P0.05,P0.01)。结论音乐无痛分娩法可明显缓解产妇分娩过程中的疼痛,缩短产程,降低剖宫产率。     

鉴别诊断临床急性肺炎病原体感染的血清标志物研究

目的 检测急性肺炎患者血清相关生物标志物含量,为建立一种快速鉴别诊断临床急性支原体肺炎、病毒性肺炎、细菌性肺炎的免疫胶体金检测法提供实验依据。方法 以临床病原学诊断明确的急性支原体肺炎,病毒性肺炎,细菌性肺炎患者和正常人血清为检测样品,采用血小板衍生生长因子（platelet-derived growth factor,PDGF）/杀菌渗透增强蛋白（bactericidal/permeability-increasing protein,BPI）酶联免疫法（enzyme-linked immunosorbent assay,ELISA）检测试剂盒检测上述血清中相关生物标志物含量。结果 （1）正常人群血清78份,急性支原体肺炎患者血清46份,病毒性肺炎和细菌性肺炎患者血清42份,其中病毒感者患者血清35份,细菌感染患者血清7份;（2）正常人群血清PDGF最大值<6 000 pg/mL;血清BPI最大值<80 ng/mL;（3）以血清PDGF值6 000 pg/mL作为鉴别诊断急性支原体感染的基线值,支原体感染患者阳性检出率为93.47%（43/46）;病毒和细菌感染患者出现支原体感染的假阳性率为7.14%（3/42）;（4）以血清BPI值80 ng/mL作为鉴别诊断急性细菌感染的基线值,细菌感染患者阳性检出率为85.71%（6/7）;病毒感染患者出现细菌感染的假阳性率为3.22%（1/31）。结论 以血清PDGF 6 000 pg/mL和BPI 80 ng/mL作为鉴别诊断不同病原体感染的基线值,对临床急性支原体肺炎、病毒性肺炎、细菌性肺炎鉴别诊断具有一定意义。     

体外循环和非体外循环下冠状动脉旁路移植术中 患者脑氧饱和度的变化趋势分析

目的 探讨体外循环和非体外循环下冠状动脉旁路移植术中患者脑氧饱和度（rScO2）的变化趋势。 方法 前瞻性选取2018 年2 月—2019 年6 月在上海市第一人民医院择期行冠状动脉旁路移植术的105 例患 者。其中,56 例接受非体外循环冠状动脉旁路移植术（OPCAB）,49 例患者接受体外循环冠状动脉旁路移植 术（CCABG）。术中分别选择T0 ～ T9 10 个时间点检测rScO2,另外记录术后7 d 内术后认知功能障碍（POCD） 发生率。结果 CCABG 组患者手术时间长于OPCAB 组（P <0.05）,术中最低鼻咽温和红细胞比容（HCT） 低于OPCAB 组（P <0.05）,术中最高血糖（Glu）和血乳酸（Lac）高于OPCAB 组（P <0.05）;但是两组术 中最低rScO2 比较,差异无统计学意义（P >0.05）。两组rScO2 在不同时间、组间、变化趋势上有差别（P <0.05）。 CCABG 组rScO2 与鼻咽温、Lac 呈正相关（r =0.229 和0.283,P =0.005 和0.002）,而OPCAB 组rScO2 只 与Lac 呈正相关（r =0.214,P =0.010）。OPCAB 组和CCABG 组POCD 发生率分别为39.29%（22/56）和 32.65%（16/49）,且CCABG 组POCD 患者最低rScO2 低于非POCD 患者（P <0.05）。结论 虽然OPCAB 与CCABG 术中患者rScO2 变化趋势有差异,但是rScO2 可能不是影响术后早期POCD 发生的主要原因。     

血清胱抑素C水平与急性缺血性脑卒中后抑郁共病状态的关系

目的 探讨血清胱抑素C水平与急性缺血性脑卒中后抑郁共病状态的关系。方法 采用前瞻性研究方法,选取2019年1月—2022年6月承德医学院附属医院100例急性缺血性脑卒中患者作为研究对象,根据发病2周时的汉密尔顿抑郁量表（HAMD-17）评分分为抑郁组与非抑郁组。对比两组临床资料及实验室指标,分析血清胱抑素C水平与急性缺血性脑卒中后抑郁共病状态的关系。结果 抑郁组大动脉粥样硬化型占比低于非抑郁组,影像学病变累及占比高于非抑郁组（P <0.05）。抑郁组与非抑郁组性别、年龄、梗死部位、吸烟史、入院时HAMD评分比较,差异无统计学意义（P>0.05）。抑郁组血清胱抑素C、同型半胱氨酸水平高于非抑郁组（P <0.05）。抑郁组与非抑郁组甘油三酯、总胆固醇、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇比较,差异无统计学意义（P>0.05）。小动脉闭塞型组胱抑素C、同型半胱氨酸水平高于其他TOAST分型组（P <0.05）。累及组胱抑素C、同型半胱氨酸水平高于未累及组（P <0.05）。多因素一般Logistic回归分析结果显示：高胱抑素C[O^R=26.330(95...     

Circ_0008039 supports breast cancer cell proliferation,migration, invasion,and glycolysis by regulating the miR‐140‐3p/SKA2 axis

Circular RNAs (circRNAs) have been shown to modulate gene expression and participate in the development of multiple malignancies. The purpose of this study was to investigate the role of circ_0008039 in breast cancer (BC). The expression of circ_0008039, miR‐140‐3p, and spindle and kinetochore‐associated protein 2 (SKA2) was detected by qRT‐PCR. Cell viability, colony formation, migration, and invasion were evaluated using methylthiazolyldiphenyl‐tetrazolium bromide (MTT) assay, colony formation assay, and transwell assay, respectively. Glucose consumption and lactate production were measured using commercial kits. Protein levels of hexokinase II (HK2) and SKA2 were determined by western blot. The interaction between miR‐140‐3p and circ_0008039 or SKA2 was verified by dual‐luciferase reporter assay. Finally, a mouse xenograft model was established to investigate the roles of circ_0008039 in BC in vivo. We found that circ_0008039 and SKA2 were upregulated in BC tissues and cells, while miR‐140‐3p was downregulated. Knockdown of circ_0008039 suppressed BC cell proliferation, migration, invasion, and glycolysis. Moreover, miR‐140‐3p could bind to circ_0008039 and its inhibition reversed the inhibitory effect of circ_0008039 interference on proliferation, migration, invasion, and glycolysis in BC cells. SKA2 was verified as a direct target of miR‐140‐3p and its overexpression partially inhibited the suppressive effect of miR‐140‐3p restoration in BC cells. Additionally, circ_0008039 positively regulated SKA2 expression by sponging miR‐140‐3p. Consistently, silencing circ_0008039 restrained tumor growth via increasing miR‐140‐3p and decreasing SKA2. In conclusion, circ_0008039 downregulation suppressed BC cell proliferation, migration, invasion, and glycolysis partially through regulating the miR‐140‐3p/SKA2 axis, providing an important theoretical basis for treatment of BC.

Abbreviations

ANOVA

analysis of variance

BC

breast cancer

circRNAs

circular RNAs

DMSO

dimethyl sulfoxide

ECAR

extracellular acidification rate

ECL

enhanced chemiluminescence

FBS

fetal bovine serum

HK2

hexokinase II

MEGM

mammary epithelial growth medium

miR‐140‐3p

microRNA‐140‐3p

MTT

methylthiazolyldiphenyl‐tetrazolium bromide

PBS

phosphate‐buffered saline

PRKAR1B

protein kinase A regulatory subunit R1‐beta

SD

standard ± deviation

SKA2

spindle and kinetochore‐associated protein 2

     

The effect of metallic Fe(ii) and nonmetallic S codoping on the photocatalytic performance of graphitic carbon nitride

The metallic Fe(ii) ion and nonmetallic S codoped g-C₃N₄ photocatalyst was synthesized through the polymerization of melamine, ferrous chloride and trithiocyanuric acid (TCA) at elevated temperature. The performance of Fe(ii)–S codoped g-C₃N₄ compounds in RhB photocatalytic degradation was found to increase 5 times. This significant enhancement in catalytic activity is probably related to the enhanced visible light adsorption and the mobility of photoinduced electron/hole pairs, attributable to bandgap narrowing and also lowering in the surface electrostatic potential compared to that of the pure g-C₃N₄ nanosheets. XRD and XPS results indicate that the Fe species binds with N-atoms to form Fe–N bonds in the state of Fe(ii) ions. Fe(ii) doping increases the specific surface area, and enhances the photoinduced electron/hole pairs illustrated by PL, EIS spectra and transient photocurrent response measurements. The theoretical results show that divalent Fe(ii) ions coordinating in the pore centre among three triazine units form discrete dopant bands and S dopants substituting the N in triazine skeletons excite much stronger delocalized HOMO and LUMO states, facilitating the migration of photogenerated charge carriers, thus enhancing the visible-light driven photocatalytic performance.

The photoinduced electrons jump more easily to the conduction band of g-C₃N₄ for the Fe impurity band locates above the valence band acting a bridge for electron transfer.