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71.
G. Geussová Dr. J. Pknicová J. apková P. Kaláb J. Moos V. V. Philimonenko and P. Hozák 《Andrologia》1997,29(5):261-268
Summary. Monoclonal antibodies Ds-1 and Ds-2 specifically labelling dog sperm acrosome were prepared by immunization of mice with acetic acid extracts of dog spermatozoa. Electron microscopy and indirect immunofluorescence localized the site of Ds-1 and Ds-2 proteins inside the acrosomal vesicle. Ds-1 antibody detected 55, 76, 115, 120 and 190kDa proteins under non-reducing conditions, and 73 kDa and 54 kDa proteins after reduction (p73/Ds-1 and p54/Ds-1). 92 kDa and 40 kDa proteins recognized by Ds-2 (p92/Ds-2 and p40/Ds-2) migrated at > 200 kDa in the absence of reducing agent. In vivo , p73/Ds-1 and p54/Ds-1 are therefore likely to be present both in free and complexed form, while all of p92/Ds-2 and p40/Ds-2 form disulfide-bonded complexes. Decrease in the rate of acrosomes stained with Ds-1 and Ds-2 was correlated with the progress of capacitation resulting in the increased rate of spontaneous acrosome reactions, as suggested by a dramatic effect of A23187. Monoclonal antibody to boar acrosin (ACR-2) recognized dog sperm acrosin homologue. A higher rate of ACR-2-negative spermatozoa was observed after capacitation and A23187 treatment compared to Ds-1 and Ds-2, indicating that proteins recognized by Ds-1 and Ds-2 are localized in a specific compartment of acrosome, distinct from acrosin and possibly representing fraction of acrosomal matrix. 相似文献
72.
新型脊柱固定器械的研制与临床应用 总被引:21,自引:0,他引:21
自1989年以来,临床应用一种新型脊柱固定器械,共68例,其中52例为胸腰椎骨折,16例为椎体滑脱。经临床观察和随访,治疗效果满意。这种新型固定器械,既可行胸腰椎骨折的固定,也可行椎体滑脱的复位。根据临床需要,该器械可行压缩、撑开、成角和中立位固定。作者强调,脊柱损伤只要有手术指征,手术时机甚为重要,应早期或立即手术,有利于脊柱骨折和椎管前方骨块的复位,早期也有利于神经功能的恢复。 相似文献
73.
Serologic epidemiology of hepatitis B viral infection and observation on the immunologic effect of hepatitis B vaccine 总被引:1,自引:0,他引:1
Q Y Ge 《中华预防医学杂志》1988,22(2):92-94
74.
尼卡地平控制性降压对家犬脊髓血流的影响 总被引:2,自引:1,他引:1
目的 观察尼卡地平控制性降压对家犬脊髓血流的影响。方法 成年杂种犬 6只 ,体重 12 5~ 16kg ,以 2 5 %硫喷妥钠麻醉。股动脉置管监测MAP。以尼卡地平 8μg·kg 1·min 1持续静脉注射控制性降压。以激光多普勒血流仪测定脊髓血流 (SCBF)。结果 降压前MAP为 (12 5 7±10 6 )mmHg ,降压后为 (72 0± 11 2 )mmHg ,平均下降 4 2 8%。降压前SCBF为 (9 80± 1 0 5 )v ,降压后为 (8 0 4± 0 96 )v ,降低幅度为 18%。结论 尼卡地平控制性降压对SCBF影响较小 ,可安全用于脊髓手术。 相似文献
75.
经单侧椎板开窗夹闭硬脊膜动静脉瘘 总被引:6,自引:1,他引:5
目的 总结经单侧椎板开窗入路夹闭硬脊膜动静脉瘘的经验。方法 回顾性分析了 5 6例经脊髓MR和脊髓血管造影确诊的硬脊膜动静脉瘘患者经单侧椎板开窗夹闭瘘口的临床资料。结果 5 4例患者术后行脊髓血管造影复查 ,显示瘘口全部消失。 38例患者术后 6个月行脊髓MR复查 ,显示脊髓周围的血管流空影完全消失 ,T2 像髓内高信号影消失或明显减少。 5 4例患者获随访 ,随访时间 3~ 36个月 ,2 4例症状完全消失 ,2 7例症状改善 ,3例无变化。结论 经单侧椎板开窗夹闭瘘口的手术方法是硬脊膜动静脉瘘的首选治疗方法。 相似文献
76.
Leonore C Dijkgraaf Gustavo Zardeneta Fabian W Cordewener Robert S b Liem John P Schmitz Lambert G m de Bont Stephen B Milam 《Journal of oral and maxillofacial surgery》2003,61(1):101-111
PURPOSE: Adhesion formation in osteoarthritis (OA) of the temporomandibular joint (TMJ) typically results in a sustained limitation of joint movement. We propose the hypothesis that free-radical-mediated crosslinking of proteins underlies this adhesion formation in affected joints. Free radicals may cause oxidative modification of proteins, creating an opportunity for the formation of intramolecular and intermolecular crosslinks via covalent bonds. This may stabilize protein aggregates, rendering them more resistant to degradation. In this study, the free-radical-mediated crosslinking of model proteins (fibrinogen and fibronectin) was investigated to test our hypothesis that free radicals contribute to adhesion formation via this mechanism in OA of the TMJ. MATERIALS AND METHODS: Physiological clot formation of fibrinogen by thrombin and free-radical-induced crosslinking of fibrinogen and of fibronectin were analyzed using spectrophotometric turbidity measurements, light-scattering techniques, polyacrylamide gel electrophoresis (PAGE), and rotary shadowing. RESULTS: Fibrinogen was shown to aggregate after free radical treatment, as detected using turbidity measurements and light-scattering techniques. Using PAGE, fibrinogen as well as fibronectin was shown to degrade under low oxidative stress. Under high oxidative stress, however, fragments from both proteins were found to be covalently crosslinked, resulting in high-molecular-weight protein aggregates. The aggregation was shown to be at random with rotary shadowing. CONCLUSION: The study shows that high oxidative stress contributes to the formation of crosslinked proteins that may serve as an initial scaffolding for the development of adhesions frequently seen in OA of the TMJ. 相似文献
77.
多巴酚丁胺负荷超声心动图及组织多普勒成像技术在诊断冠心病中的临床应用 总被引:1,自引:0,他引:1
目的探讨多巴酚丁胺负荷超声心动图(DSE)和组织多普勒成像技术(TDI)在冠心病(CHD)诊断中的应用价值,以寻求一种敏感的、无创的CHD诊断方法。方法选择胸闷或胸痛怀疑CHD患者40例,应用TDI测量静息状态(Rest)下前壁中段收缩峰值速度(Vs),以及多巴酚丁胺峰值负荷状态(Peak)下前壁中段Vs,同时测量静息心率和负荷心率;所有入选对象均进行冠脉造影,并根据左前降支狭窄程度是否大于等于50%分为左前降支狭窄组(LST组)与左前降支非狭窄组(non-LST组)。分别比较Rest和Peak前壁中段LST组与non-LST组平均Vs差别等。结果Rest前壁中段LST组与non-LST组Vs差别无显著性意义;但Peak前壁中段LST组与non-LST组差别有显著性意义。结论DSE结合TDI是诊断冠心病有价值的定量分析方法。DSE在诊断CHD时的敏感性、准确性明显优于静息状态超声心动图。 相似文献
78.
采用显微外科技术行阴囊纵膈皮瓣尿道下裂修复术18例,均获成功。其手术要点为:将阴茎腹侧纤维索条切除,充分伸展阴茎,皮瓣保留足够长度与宽度,克保血运;在显微镜下采用7-0尼龙丝线缝合,形成皮管,不缝皮肤,只缝皮下。硅胶管支架引流,加压包扎阴茎。本文对手术成功的原因进行了初步讨论。 相似文献
79.
Elke Wezenberg Robert Jan Verkes Ge S F Ruigt Wouter Hulstijn Bernard G C Sabbe 《Neuropsychopharmacology》2007,32(6):1272-1283
Ampakines act as positive allosteric modulators of AMPA-type glutamate receptors and facilitate hippocampal long-term potentiation (LTP), a mechanism associated with memory storage and consolidation. The present study investigated the acute effects of farampator, 1-(benzofurazan-5-ylcarbonyl) piperidine, on memory and information processes in healthy elderly volunteers. A double-blind, placebo-controlled, randomized, cross-over study was performed in 16 healthy, elderly volunteers (eight male, eight female; mean age 66.1, SD 4.5 years). All subjects received farampator (500 mg) and placebo. Testing took place 1 h after drug intake, which was around Tmax for farampator. Subjects performed tasks assessing episodic memory (wordlist learning and picture memory), working and short-term memory (N-back, symbol recall) and motor learning (maze task, pursuit rotor). Information processing was assessed with a tangled lines task, the symbol digit substitution test (SDST) and the continuous trail making test (CTMT). Farampator (500 mg) unequivocally improved short-term memory but appeared to impair episodic memory. Furthermore, it tended to decrease the number of switching errors in the CTMT. Drug-induced side effects (SEs) included headache, somnolence and nausea. Subjects with SEs had significantly higher plasma levels of farampator than subjects without SEs. Additional analyses revealed that in the farampator condition the group without SEs showed a significantly superior memory performance relative to the group with SEs. The positive results on short-term memory and the favorable trends in the trail making test (CTMT) are interesting in view of the development of ampakines in the treatment of Alzheimer's disease and schizophrenia. 相似文献
80.
我院是山西省规模最大的集医疗、教学、科研、预防为一体的综合性三级甲等医院,共有58个临床和医技科室、34个病房。各临床科室在日常诊疗过程中都要使用大量的医疗仪器,其中,属于国家强检范围内的仪器共有788台,种类也已由过去的13种上升到现在的18种。根据我院实际情况,建立了一套全方位、一条龙式的医院计量管理系统,使我院的计量器具管理更加规范化、科学化、现代化,解决了长期以来医院计量管理工作的复杂、烦琐、效率低下、漏检、漏查等问题,提高了计量管理工作的质量和效率。 相似文献