普伐他汀对成功冠脉成形术患者的影响

为探讨普伐他汀对成功冠脉成形术患者术后6月再狭窄发生率及心脏事件的影响,将48例患者(男38例,女10例,年龄60.4±7.9岁)随机从手术当晚起口服普伐他汀(每日10mg)或安慰剂, 随访平均6月,观察心脏事件,随访结束时复查冠脉造影和血脂.结果显示术后6个月时普伐他汀组血脂参数明显改善,以总胆固醇和低密度脂蛋白胆固醇下降最为明显(分别为11.4%和14.4%,P<0.01).冠脉造影显示,两组再狭窄发生率无明显差异(40.9%对38.5%,P>0.05).心脏事件发生率普伐他汀组明显低于对照组(11.5%对36.4%,P<0.01),普伐他汀药物不良反应少.本组结果显示普伐他汀可以减少术后6个月内心脏事件的发生.     

人免疫缺陷病毒HB-J株的分离和生物学特性研究

目的：分离和鉴定我国流行的人免疫缺陷病毒（ｈｕｍａｎ ｉｍｍｕｎｏｄｅｆｉｃｉｅｎｃｙ ｖｉｒｕｓ,ＨＩＶ）毒株,研究我国ＨＩＶ毒株的生物学特性。方法：周外用血单个核细胞（ｐｅｒｉｐｈｅｒａｌ ｂｌｏｏｄ ｍｏｎｏｎｕｃｌｅａｒ ｃｅｌｌｓ,ＰＢＭＣ）共培养的方法分离ＨＩＶ,用观察细胞病变、检测Ｐ２４抗原和前病毒ＤＮＡ的方法监测病毒生长的情况。结果：在建立共培养的第１２天观察到明显的细胞病变,主要表面为大泡样细胞和多核巨细胞。接种第９天细胞培养上清液的Ｐ２４抗原由阴性转为阳性,并且浓度迅速增加,接种第１２天浓度即达到１７８０ｐｇ／ｍｌ,第１４天达到峰值,为２３２０ｐｇ／ｍｌ。但该株病毒的ＰＢＭＣ培养上清液转种于ＭＴ４细胞后,培养上清液Ｐ２４抗原和前病毒ＤＮＡ始终为阴性且不出现细胞病变。结论：用ＰＢＭＣ共培养的方     

两种β阻滞剂治疗轻、中度原发性高血压的比较

目的 :比较国产卡维地洛与柳胺苄心定治疗轻、中度原发性高血压患者的降压疗效和安全性。方法 :轻中度原性高血压患者 5 0例 ,随机分为卡维地洛组 (n=2 5 )与柳胺苄心定组 (n =2 5 ) ,分别口服卡维地洛 10mgbid,柳胺苄心定 5 0mgbid (均 8AM与 8PM)。治疗 2周后卡维地洛组 9例与柳胺苄心定组 4例因血压降低不满意 (舒张压>90mmHg)而分别增加剂量到 2 0mg和 10 0mgbid。共观察4周。结果 :卡维地洛组舒张压下降 11.2± 6 .7mmHg(P <0 0 1) ,收缩压下降 6 .9± 16 .2mmHg。柳胺苄心定组舒张压下降 1.1± 6 .2mmHg(P <0 0 1)。卡维地洛组总有效率 88%与柳胺苄心定组 84%相似。两组用药前后心率、血糖及血清胆固醇、甘油三酯均无明显变化 ;卡维地洛组HDL虽明显下降 ,但仍在正常范围内 ,不良反应少。结论 :国产卡维地洛治疗高血压的疗效与对照药柳胺苄心定相似 ,其疗效确切 ,不良反应少 ,耐受性好。     

Ⅱ型糖尿病胃轻瘫病人与幽门螺杆菌感染

目的　了解Ⅱ型糖尿病胃轻瘫病人幽门螺杆菌感染情况。方法　用快速尿素酶试验、组织学检查、细菌培养法检测 30 2 5例病人幽门螺杆菌感染情况。结果　Ⅱ型糖尿病胃轻瘫病人幽门螺杆菌感染率为 78 6 1% (2 378/ 30 2 5 ) ,其中性别及季节之间无显著差异性。结论　幽门螺杆菌感染可能与Ⅱ型糖尿病胃轻瘫有关。     

Cbln3, a novel member of the precerebellin family that binds specifically to Cbln1.

Precerebellin (Cbln1) is the precursor of the brain-specific hexadecapeptide cerebellin. Although cerebellin has properties of a conventional neuropeptide, its function is controversial because Cbln1 has structural features characteristic of circulating atypical collagens. Cbln1 is related to the three subunits of the complement C1q complex. Therefore, we hypothesized that Cbln1 participated in analogous heteromeric complexes with precerebellin-related proteins. Using LexA-Cbln1 as bait in a yeast two-hybrid screen, we isolated a cDNA encoding a novel Cbln1-related protein, designated Cbln3. The gene encoding cbln3 had the same intron-exon structure as cbln1 but mapped to a different mouse chromosome (14). The deduced amino acid sequence of Cbln3 was 55% identical to Cbln1 and also contained a C1q signature domain and signal sequence for secretion. In addition to binding avidly to Cbln3, Cbln1 also formed homomeric complexes. In contrast, Cbln3 homomeric association was weak. These interactions exhibited specificity because C1qB bound to neither Cbln1 nor Cbln3. Like cbln1, cbln3 was expressed in the cerebellum and dorsal cochlear nucleus in which it was detected in granule neurons. Because Cbln1 and Cbln3 are coexpressed in the brain and interact avidly, they may function as a secreted heteromeric complex in vivo.     

T G D牙髓治疗剂的药敏试验

目的 检测ＴＧＤ牙髓治疗剂对感染根管常见厌氧菌的抑菌和杀菌效果。方法 采用药物平皿试验方法测量ＴＧＤ溶液对产黑色素类杆菌等８种厌氧菌的抑菌环大小：ＴＧＤ溶液与８种菌接触３ｍｉｎ,５ｍｉｎ后观察菌落生长情况,上述试验均与ＦＣ,ＣＰ和戊二醛进行比较。结果 ＴＧＤ对８种细菌的抑菌环为２２～４８ｍｍ,与ＦＣ一致而明显大于ＣＰ（１０～１８ｍｍ）和戊二醛（Ｐ〈０．０１）,其３ｍｉｎ,５ｍｉｎ的杀菌效果较ＣＰ和戊二醛强,结论 ＴＧＤ牙髓治疗剂对厌氧菌有较强的抑菌和杀菌作用。     

冠状动脉粥样硬化的超高速CT检测与病理学对照:Ⅱ-钙化参数与管腔狭窄之间的关系

目的为探讨超高速CT检测的冠状动脉钙化与冠状动脉管腔狭窄之间的关系。方法从8例压力灌注固定后的心脏标本中分离出20支冠状动脉,拉直后,超高速CT扫描;每支冠状动脉分成相应的3mm血管段,组织切片,地衣红染色,Leica图像分析仪上计算冠状动脉管腔面积狭窄率。结果在484个血管段中,无粥样病变血管段的钙化率、钙化面积、钙化积分(0,0,0)明显低于有粥样病变的血管段(28％,1.74％和6．73,P     

胆囊结石及癌变过程中肿瘤坏死因子的变化

目的 探讨肿瘤坏死因子(TNF-a)在胆囊结石及癌变过程中的变化。方法 用放射免疫法对53例胆囊结石,9例胆囊癌血清及胆汁中TNF水平进行检测。结果 血清及胆汁中TNF水平(ng／mL)胆囊癌组(1．35±0.28,10．28±3.23)较胆石症组(0．93±0.23,3．38±1．11)及对照组(0．67±0．17,2．12±0．55)均显著升高(P     

血乳酸和APACHEⅡ评分在评估重症胰腺炎患者病情预后中的价值

目的 分析探讨重症胰腺炎患者血乳酸水平与APACHEⅡ评分的相关性及指导预后的意义。方法 回顾性分析45例入住笔者医院重症医学科（intensive care unit,ICU）中重症胰腺炎患者的临床资料,根据其预后将患者分为存活组和死亡组,比较血乳酸水平与APACHEⅡ分值之间的关系,分析上述指标与患者预后之间的关系。结果 死亡组患者的血乳酸水平、APACHEⅡ评分明显高于存活组（P<0.05）,APACHEⅡ评分>30分组血乳酸水平明显高于21~30分组（P<0.05）,APACHEⅡ评分21~30分组血乳酸水平明显高于≤10及其11~20分组（P<0.05）;血乳酸水平和APACHEⅡ分值的相关性呈显著正相关（r=0.995,P<0.01）。结论 血乳酸水平、APACHEⅡ评分均与重症胰腺炎患者病情严重程度相关,在该类患者预后判断中具有一定的临床指导意义。     

Detection techniques of biological and chemical Hall sensors

Integrated magnetic Hall effect sensors have been widely used in people''s daily life over the past decades, and still are gaining enormous attention from researchers to establish novel applications, especially in biochemistry and biomedical healthcare. This paper reviews, classifies, compares and concludes state-of-the-art integrated Hall magnetic sensors in terms of cost, power, area, performance and application. Current applications of the Hall sensors such as detecting magnetic nanoparticles (MNPs) labeled on biomolecule, monitoring blood pulse wave velocity, characterizing soft biological materials, controlling syringe injection rate and eye surgery by training systems, and assisting magnetic resonance imaging (MRI) will be discussed comprehensively and future applications and trends will be highlighted. This review paper will introduce Hall sensor''s advantages such as simple design and technology of manufacturing, low cost, low power consumption, possibility of the miniaturizing, noninvasive and room temperature measurement, with respect to the other magnetic sensing systems and methods.

Integrated magnetic Hall effect sensors have been widely used in people''s lives over the past decades. They are still gaining enormous attention from researchers to establish novel applications, especially in biochemistry and biomedical healthcare.