Cbln3, a novel member of the precerebellin family that binds specifically to Cbln1.

Precerebellin (Cbln1) is the precursor of the brain-specific hexadecapeptide cerebellin. Although cerebellin has properties of a conventional neuropeptide, its function is controversial because Cbln1 has structural features characteristic of circulating atypical collagens. Cbln1 is related to the three subunits of the complement C1q complex. Therefore, we hypothesized that Cbln1 participated in analogous heteromeric complexes with precerebellin-related proteins. Using LexA-Cbln1 as bait in a yeast two-hybrid screen, we isolated a cDNA encoding a novel Cbln1-related protein, designated Cbln3. The gene encoding cbln3 had the same intron-exon structure as cbln1 but mapped to a different mouse chromosome (14). The deduced amino acid sequence of Cbln3 was 55% identical to Cbln1 and also contained a C1q signature domain and signal sequence for secretion. In addition to binding avidly to Cbln3, Cbln1 also formed homomeric complexes. In contrast, Cbln3 homomeric association was weak. These interactions exhibited specificity because C1qB bound to neither Cbln1 nor Cbln3. Like cbln1, cbln3 was expressed in the cerebellum and dorsal cochlear nucleus in which it was detected in granule neurons. Because Cbln1 and Cbln3 are coexpressed in the brain and interact avidly, they may function as a secreted heteromeric complex in vivo.     

T G D牙髓治疗剂的药敏试验

目的 检测ＴＧＤ牙髓治疗剂对感染根管常见厌氧菌的抑菌和杀菌效果。方法 采用药物平皿试验方法测量ＴＧＤ溶液对产黑色素类杆菌等８种厌氧菌的抑菌环大小：ＴＧＤ溶液与８种菌接触３ｍｉｎ,５ｍｉｎ后观察菌落生长情况,上述试验均与ＦＣ,ＣＰ和戊二醛进行比较。结果 ＴＧＤ对８种细菌的抑菌环为２２～４８ｍｍ,与ＦＣ一致而明显大于ＣＰ（１０～１８ｍｍ）和戊二醛（Ｐ〈０．０１）,其３ｍｉｎ,５ｍｉｎ的杀菌效果较ＣＰ和戊二醛强,结论 ＴＧＤ牙髓治疗剂对厌氧菌有较强的抑菌和杀菌作用。     

冠状动脉粥样硬化的超高速CT检测与病理学对照:Ⅱ-钙化参数与管腔狭窄之间的关系

目的为探讨超高速CT检测的冠状动脉钙化与冠状动脉管腔狭窄之间的关系。方法从8例压力灌注固定后的心脏标本中分离出20支冠状动脉,拉直后,超高速CT扫描;每支冠状动脉分成相应的3mm血管段,组织切片,地衣红染色,Leica图像分析仪上计算冠状动脉管腔面积狭窄率。结果在484个血管段中,无粥样病变血管段的钙化率、钙化面积、钙化积分(0,0,0)明显低于有粥样病变的血管段(28％,1.74％和6．73,P     

胆囊结石及癌变过程中肿瘤坏死因子的变化

目的 探讨肿瘤坏死因子(TNF-a)在胆囊结石及癌变过程中的变化。方法 用放射免疫法对53例胆囊结石,9例胆囊癌血清及胆汁中TNF水平进行检测。结果 血清及胆汁中TNF水平(ng／mL)胆囊癌组(1．35±0.28,10．28±3.23)较胆石症组(0．93±0.23,3．38±1．11)及对照组(0．67±0．17,2．12±0．55)均显著升高(P     

血乳酸和APACHEⅡ评分在评估重症胰腺炎患者病情预后中的价值

目的 分析探讨重症胰腺炎患者血乳酸水平与APACHEⅡ评分的相关性及指导预后的意义。方法 回顾性分析45例入住笔者医院重症医学科（intensive care unit,ICU）中重症胰腺炎患者的临床资料,根据其预后将患者分为存活组和死亡组,比较血乳酸水平与APACHEⅡ分值之间的关系,分析上述指标与患者预后之间的关系。结果 死亡组患者的血乳酸水平、APACHEⅡ评分明显高于存活组（P<0.05）,APACHEⅡ评分>30分组血乳酸水平明显高于21~30分组（P<0.05）,APACHEⅡ评分21~30分组血乳酸水平明显高于≤10及其11~20分组（P<0.05）;血乳酸水平和APACHEⅡ分值的相关性呈显著正相关（r=0.995,P<0.01）。结论 血乳酸水平、APACHEⅡ评分均与重症胰腺炎患者病情严重程度相关,在该类患者预后判断中具有一定的临床指导意义。     

Effect of low-degree astigmatism on the objective visual quality

AIM: To evaluate the effect of low-degree astigmatism on objective visual quality through the Optical Quality Analysis System (OQAS). METHODS: This study enrolled 46 participants (aged 23 to 30y, 90 eyes) with normal or corrected-to-normal vision. The cylindrical lenses (0, 0.5, 0.75, 1.0, and 1.25 D) were placed at the axial direction (180°, 45°, 90°, and 135°) in front of the eyes with the best correction to form 16 types of regular low-degree astigmatism. OQAS was used to detect the objective visual quality, recorded as the objective scattering index (OSI), OQAS values at contrasts of 100%, 20%, and 9% predictive visual acuity (OV100%, OV20%, and OV9%), modulation transfer function cut-off (MTFcut-off) and Strehl ratio (SR). The mixed effect linear model was used to compare objective visual quality differences between groups and examine associations between astigmatic magnitude and objective visual quality parameters. RESULTS: Apparent negative relationships between the magnitude of low astigmatism and objective visual quality were observed. The increase of OSI per degree of astigmatism at 180°, 45°, 90°, and 135° axis were 0.38 (95%CI: 0.35, 0.42), 0.50 (95%CI: 0.46, 0.53), 0.49 (95%CI: 0.45, 0.54) and 0.37 (95%CI: 0.34, 0.41), respectively. The decrease of MTFcut-off per degree of astigmatism at 180°, 45°, 90°, and 135° axis were -10.30 (95%CI: -11.43, -9.16), -12.73 (95%CI: -13.62, -11.86), -12.75 (95%CI: -13.79, -11.70), and -9.97 (95%CI: -10.92, -9.03), respectively. At the same astigmatism degree, OSI at 45° and 90° axis were higher than that at 0° and 135° axis, while MTFcut-off were lower. CONCLUSION: Low astigmatism of only 0.50 D can significantly reduce the objective visual quality.     

一起天津市集体单位内新型冠状病毒肺炎聚集性疫情流行病学调查

目的 对天津市一起新型冠状病毒肺炎（COVID-19）聚集性疫情进行详细调查和分析,旨在为COVID-19的流行病学特征提供更多证据,并对前期基于有限证据和经验实施的防控措施进行评估。方法 采用描述性研究方法对一起发生在集体单位内的聚集性病例进行三间分布及其他流行病学特征描述。结果 自第一例指示病例于1月15日发病后,该单位相继发现10例确诊病例,且疫情从单位传播到4个家庭内,致7人发病。17例病例年龄中位数为55（19~79）岁;其中10例职工病例全部为男性,7例家庭聚集病例中男性3例,女性4例。职工病例中,8例办公地点在CW车间,2例在行政办公楼。病例的暴露-发病间隔中位数为4 d,其中职工病例暴露-发病间隔中位数为4.5 d,家庭聚集病例为4 d。初期发病未实施隔离措施的病例发病-就诊间隔中位数为4 d,发病期间开始居家隔离的病例发病-就诊间隔中位数为2.5 d,发病前开始居家隔离的病例发病-就诊间隔中位数为0.5 d。结论 COVID-19传播过程中存在单位和家庭聚集性,在疫情发生初期,准确、迅速的采取封控措施完全可以阻止COVID-19聚集性疫情的大范围扩散。     

比较4F和6F造影导管经股动脉行冠状动脉造影

目的 比较使用4F及6F造影导管经股动脉径路行冠状动脉造影的可行性。方法 把疑诊冠心病病人409例分成两组,分别使用4F造影导管178例和6F造影导管231例行冠状动脉及左心室造影,对比两组病人手术成功率,术后止血压迫时间、开始活动时间、局部及全身并发症发生率等。结果 两组病例造影成功率及完成造影时间大致相同,4F组造影剂使用量、术后止血压迫时间、卧床时间、穿刺点伤口皮下血肿形成、皮下淤斑、迷走神经反射发生率均明显低于6F组（P＜0.01）。结论 与6F动脉鞘及造影导管比较,使用4F动脉鞘及造影导管经股动脉经路行冠状动脉造影具有方便、可靠、损伤小、并发症少,病人可早期活动、痛苦小、容易接受的优点,可作为诊断性造影、门诊造影和介入术后复查首选方法。     

督脉刺法治疗脑血管性痴呆的临床研究

目的观察督脉刺法治疗血管性痴呆的临床疗效。方法将85例血管性痴呆患者随机分为2组:治疗组用针刺加哈伯因治疗,对照组仅用哈伯因治疗,治疗前后用简易智能量表(MMSE)、日常生活能力量表(ADL)进行评分。结果2组治疗后MMSE积分、ADL积分均明显提高,且治疗组提高程度优于对照组。结论督脉刺法能显著改善血管性痴呆患者的认知功能及日常生活能力,临床疗效显著。     

心肌梗死前心绞痛和白细胞计数对急性心肌梗死患者左室射血功能的影响

目的探讨心肌梗死前心绞痛和白细胞计数与急性心肌梗死(AMI)后左室射血分数(LVEF)的关系。方法选择48例初发的前壁ST段抬高AMI患者,均在入院时检测白细胞计数,入院后30 min内溶栓治疗,记录AMI发生前24 h内出现的心绞痛,在入院时和出院前测LVEF。结果白细胞计数升高与出院前低LVEF有关;心肌梗死前心绞痛与出院前LVEF的增加有关,有心肌梗死前心绞痛患者白细胞计数更低。Logistic回归分析显示白细胞计数、心肌梗死前心绞痛均与LVEF密切相关。结论白细胞计数升高和心肌梗死前心绞痛的缺乏与再灌注治疗后LVEF受损有关,心肌梗死前心绞痛可能通过抗炎作用使LVEF改善。