Generation of HLA-DP transgenic pigs for the study of xenotransplantation.

The shortage of human organs has prompted scientists to seek xenogeneic sources of donors. To date, DAF, MCP, and CD59 transgenic pigs have been generated to inhibit hyperacute rejection. However, besides hyperacute rejection, acute and chronic rejection must also be considered in the use of porcine organs for xenotransplantation. The role of HLA-II in transgenic xeno-organ transplantation remains to be elucidated. By microinjecting 1655 embryos, we have generated one stillborn HLA-DR and two live HLA-DP transgenic pigs: P113-7 (male, carrying one copy of exogene) and P113-8 (female, carrying 2-3 copies of exogenes). The gene status of the live transgenic pigs was confirmed by PCR, Southern blot, and PCR product sequencing analysis. The expression of transgenes in these transgenic pigs were confirmed by RT-PCR analysis and immunohistochemical staining of frozen sections of ear tissue.     

静注负荷量苯巴比妥治疗新生儿惊厥(附100例临床分析)

对100例新生儿惊厥采用负荷量苯巴比妥静注治疗,在病因治疗的基础上,苯巴比妥一般按20～30mg／kg,最高达35mg／kg,短时间内静脉给药,12小时后给予维持量。总有效率为94％。疗效出现时间最短3分钟,最长55分钟。临床观察结果表明该疗法对新生儿惊厥不仅起效快,疗效明显,而且到作用小,使用安全可靠,是治疗新生儿惊厥的首选方法。     

痢特灵药疹患者的免疫学观察及发病情况调查

痢特灵药疹患者外周血的免疫学检测显示:发疹期B细胞数增加,功能增强和CIC增高:T细胞数减少和功能降低.临床上有一定潜伏期,皮损以尊麻垂为主,伴内脏损害,提示此类药疹表现为l型血清病样反应.非特异性LTT与CIC的变化与病情间呈平行关系.治疗服用痢特灵的发疹率明显高于预防服药者.     

鱼肝引起的烫伤样皮肤综合征一例报告

烫伤样皮肤综合征(Scalded Skin Syndrome)乃一严重而少见之皮肤病,由进食鱼肝引起者国内尚未见有报道,最近作者遇见一例,报告如下.     

Local Renin-Angiotensin System Activation and Myofibroblast Formation in Graft Versus Host Disease–Associated Conjunctival Fibrosis

PurposeThe present study was designed to investigate the role of myofibroblast transdifferentiation and the conjunctival renin–angiotensin system (RAS) in the pathogenesis of graft-versus-host disease (GVHD)–associated conjunctival fibrosis.MethodsA mouse model of major histocompatibility-matched allogeneic transplantation was used to induce GVHD, with male B10.D2 mice as donors and female BALB/c mice as recipients. Male BALB/c to female BALB/c syngeneic transplantation was used as control. Y chromosome staining in the spleen cells obtained from female recipient mice was used to confirm engraftment. The phenol red thread test and fluorescein staining were used to quantify tears and corneal keratopathy. Eyes were harvested at 4 and 8 weeks after the transplant for alpha-smooth muscle actin (α-SMA), angiotensinogen, and angiotensin-converting enzyme (ACE) immunostaining. Conjunctiva was harvested for gene expression quantification of α-SMA, angiotensinogen, and ACE.ResultsMore than 80% of the spleen cells in the recipient mice were chromosome Y positive, thus conforming successful engraftment. A significant decrease in tear secretion and a marked increase in corneal keratopathy score after allogeneic transplantation indicated the onset of ocular GVHD in these mice. A significant increase in α-SMA gene expression and the presence of a large number of α-SMA–positive cells was noted in the bulbar orbital conjunctiva of mice after allogeneic transplantation. Allogenic transplantation also caused a significant increase in the gene expression and protein expression of angiotensinogen and ACE in the subconjunctival eyelid area.ConclusionsResults of the present study demonstrate that GVHD-associated conjunctival fibrosis is accompanied by myofibroblast formation and activation of the local conjunctival RAS.     

孕期人乳头状瘤病毒亚临床感染的母婴传播及对胎婴儿的影响

目的　探讨孕中期、孕晚期人乳头状瘤病毒亚临床感染的母婴垂直传播情况及对胎婴儿的影响。方法　采用多聚酶链反应技术对 42例孕中期、32例孕晚期妇女的宫颈分泌物、母血、胎盘、羊水、脐血、新生儿咽部分泌物标本进行HPV - 6、11、16、18型DNA检测。结果　孕中期 :12例宫颈分泌物HPV阳性 (12 / 42 ) ,11例母血HPV阳性 (11/ 42 ) ,9例胎盘组织HPV阳性 (9/ 42 ) ,2 2例羊水标本中 ,仅 1例羊水HPV阳性 (1/ 2 2 ) ;孕晚期 :2 3例宫颈分泌物HPV阳性 (2 3/ 32 ) ,18例母血HPV阳性 (18/ 32 ) ,12例胎盘组织HPV阳性 (12 / 32 ) ,31例羊水及脐血标本中 ,4例羊水HPV阳性 (4/ 31) ,12例脐血HPV阳性 (12 / 31)。 17例异常胎婴儿中有 3例胎儿窘迫、1例新生儿高胆红素血症及 1例无脑畸形儿的脐血或胎盘组织HPV阳性。结论　孕期HPV感染的母婴传播不但可经产道直接接触传播 ,还可经血液、羊水及胎盘发生宫内传播 ,其宫内传播率的高低主要与母血的HPV感染相关。新生儿脐血的HPV感染主要与母血的HPV感染相关 ,而产时新生儿咽部的HPV感染主要与宫颈分泌物HPV感染相关。妊娠期HPV感染可能与胎儿窘迫、新生儿高胆红素血症、胎儿畸形有关     

全髋关节置换术与神经损伤

目的　探讨全髋关节置换术后股神经与坐骨神经损伤发生的原因。方法　研究分析了我院 1983～ 1998年所施行的 5 87个全髋关节置换术。结果　共有 9例神经损伤 ,8例为坐骨神经损伤 ,1例为股神经损伤 ,均为非完全性损伤。肢体延长与神经损伤之间无明显关系 ,两者统计学处理无显著差异。而手术的难易程度与神经损伤之间有非明显的关系 ,两者之间有显著差异。结论　我们认为大多数神经损伤是由于手术中直接或间接的机械损伤所致。     

高效液相色谱法测定刺五加注射液的含量

的建立刺五加注射液中芦丁水解产物槲皮素的高效液相色谱法。方法以Alltima C     

电视胸腔镜下动脉导管钳闭手术方法及疗效探讨

目的 :探讨电视胸腔镜下钳闭动脉导管的手术方法 ,总结近期及远期疗效。　　方法 :36例患者 ,平均年龄 9.0 8± 4.96岁 ,平均动脉导管直径 0 .6 2± 0 .2 5 cm,73.0 %为管状型 ,其余为漏斗型 ,均采用大号钛夹钳闭。　　结果 :所有患者均成功地在电视胸腔镜下完全钳闭动脉导管 ,无导管损伤出血、喉返神经损伤等并发症 ,术后超声心动图复查 ,无残余分流。术后最长已随访 3年 ,均未发现导管再通现象。　　结论 :电视胸腔镜下钳闭动脉导管可取得良好的近期与远期手术效果 ,值得推广应用。