健康教育在糖尿病周围神经血管病变中的应用观察

目的探讨健康教育在糖尿病周围神经血管病变中的应用效果。方法选取2011-09—2014-09我科收治的112例糖尿病周围神经血管病变患者为研究对象,按照随机分配原则将其分为对照组、研究组,每组56例。对照组实施一般护理方案,研究组实施一般护理+健康教育方案。观察对比2组血糖控制情况、心理状况评分及生化指标、疾病认知掌握情况。结果研究组血糖控制情况优于对照组,差异有统计学意义(P0.05)。研究组SAS评分、SDS评分均高于对照组,研究组心理状态优于对照组,差异有统计学意义(P0.05)。结论将健康教育用于糖尿病周围神经血管病变患者的临床护理中,可显著改善患者心理状态,提升患者疾病认知度,对患者血糖控制、疾病恢复十分有益,值得推广应用。     

Interleukin-11 stimulates multilineage progenitors, but not stem cells, in murine and human long-term marrow cultures

Interleukin-11 (IL-11) is a bone marrow microenvironment-derived growth factor with pleiotropic effects on a variety of hematopoietic cells. To more accurately assess the effects of IL-11 on stem and progenitor compartments within the hematopoietic microenvironment (HM), we added recombinant human (rh) IL-11 to human and murine long-term bone marrow cultures (LTMC) and analyzed primitive (high proliferative potential- colony forming cells [HPP-CFC], long-term culture-initiating cells [LTC- IC], and long-term reconstituting stem cells) and progenitor (day 12 colony forming unit-spleen [CFU-S12], colony forming unit-megakaryocyte [CFU-Mk] and colony forming unit-granulocyte/macrophage [CFU-GM]) compartments throughout the duration of the cultures. rhIL-11 (100 ng/mL) added twice weekly resulted in significantly increased nonadherent (NA) cellularity, CFU-GM, and CFU-Mk production in human LTMC. Addition of rhIL-11 to murine LTMC was associated with a 5- to 40- fold increase in CFU-GM and a four- to 20-fold increase in day 12 CFU-S in NA cells. However, IL-11 had no significant effect on total HPP-CFC concentration and decreased the size of the more primitive stem/progenitor compartment as evidenced by both decreased LTC-IC frequency in human LTMC and decreased frequency of long-term reconstituting stem cells in murine LTMC. These data suggest that IL-11 may increase commitment of stem cells into a multipotential progenitor compartment.     

化学合成小干扰RNA对PC12细胞NgR表达的影响

目的 Nogo及Nogo-66受体(Ng R)对抑制中枢神经系统再生有重要的作用。本文主要观察化学合成Ng R特异性小干扰RNA(si RNA)对具有神经元分化潜能的克隆细胞系PC12细胞Ng R m RNA和蛋白表达的影响。方法培养大鼠肾上腺嗜铬瘤细胞PC12细胞,神经生长因子(NGF)诱导使其分化为类神经细胞,化学合成一段针对大鼠Ng R基因编码区的si RNA,用阳离子脂质体使之转染类神经细胞,转染后48 h,采用实时荧光定量PCR检测m RNA水平;并采用蛋白免疫印迹检测Ng R蛋白表达的变化,检测干扰的效果。结果荧光定量PCR结果显示:转染后48 h Ng R m RNA水平明显下降,差异具有显著性(P<0.05);同时蛋白免疫印迹结果显示,Ng R蛋白表达水平与对照组比较也降低,差异同样具有显著性(P<0.05)。结论化学合成Ng R特异性si RNA可以实现大鼠神经细胞内源性Ng R基因沉默。     

转移性乳腺癌的化疗策略

乳腺癌是女性最常见的恶性肿瘤之一,其发病率高居女性肿瘤疾病首位,全世界每年约有50万妇女死于乳腺癌。晚期乳腺癌的治疗目标是控制疾病、缓解症状、延长患者生存时间、提高患者生存质量、减少治疗相关毒性。本文介绍了晚期乳腺癌化疗的相关指征、不同化疗方案的选择、后续的维持治疗方案在转移性乳腺癌患者中的应用情况。