多西他赛联合卡培他滨方案在新辅助化疗三阴乳腺癌与非三阴乳腺癌中的近期疗效观察

目的探讨三阴乳腺癌(triple-negative breast cancer,TNBC)与非三阴乳腺癌患者应用多西他赛联合卡培他滨(DX)方案行新辅助化疗的临床疗效及不良反应。方法选择2008年7月—2013年8月收治的102例乳腺癌患者,其中TNBC 27例,非TNBC75例,均应用DX方案新辅助化疗。观察临床疗效和毒副反应发生情况。结果TNBC患者总有效率与非TNBC患者比较差异无统计学意义(P>0.05)。TNBC患者病理完全缓解率、临床完全缓解率高于非TNBC患者(P<0.01,P<0.05)。毒副反应主要表现为胃肠道反应、骨髓抑制及手足综合征等,TNBC与非TNBC患者毒副反应发生率比较差异无统计学意义(P>0.05)。结论 TNBC患者较非TNBC患者对DX方案新辅助化疗更敏感,且未增加毒副反应发生率。     

深低温保存羊膜在眼睑成形术中的应用

观察和评价羊膜在眼睑缺损成形术中的应用价值。方法采用保存人羊膜移植用于各种原因引起的眼睑缺损部位替自体球结膜移植,成形再造眼睑共２６例２６只眼。结果２６只眼无１例感染,１０天后移植的羊膜变透明,缝合处结膜向羊膜上爬行,１个月后新生的结膜上皮完全覆盖移植区。     

痹祺胶囊对兔骨关节炎软骨代谢的作用研究

目的：观察痹祺胶囊对骨关节炎软骨代谢的作用。方法：以Hulth法复制兔膝骨关节炎模型,对软骨组织行Mankin标准评分,设置空白组、假手术组,观测各组兔血清及尿液的羟脯氨酸含量,并设痹祺胶囊低、中、高浓度组及西药组以了解其疗效。结果：各治疗组间Mankin标准评分水平无差异,痹祺胶囊各剂量组血清、尿羟脯氨酸含量与模型组比较差异均有显著意义（P〈0.05）。结论：痹祺胶囊可通过促进骨修复或抑制软骨破坏而减低兔骨关节炎模型体内羟脯氨酸含量,对骨关节炎软骨代谢有明确的改善作用。     

Silencing of Nesprin‐2 inhibits the differentiation of myofibroblasts from fibroblasts induced by mechanical stretch

Mechanical force plays a pivotal role in the pathogenesis of hypertrophic scar (HTS). Dermal fibroblasts and myofibroblasts are the key cells involved in HTS. Myofibroblasts in HTS possess different biochemical and biophysical characteristics by which myofibroblasts are often distinguished from fibroblasts. The role of mechanotransducers outside the nucleus in the pathogenesis of HTS has been reported in many studies. However, the role of Nesprin‐2 in HTS is not clear. Hence, we aim to construct a cell model of HTS and explore the role of Nesprin‐2 in this process. Myofibroblasts and fibroblasts were isolated from HTS and healthy skin tissues of the same patient. Fibroblasts were exposed to cyclic stretch with 10% magnitude and a frequency of 0.1 Hz for 3 days, 5 days, and 7 days, respectively. After the cell model was confirmed, fibroblasts transfected with siRNA targeting human Nesprin‐2 were exposed to cyclic stretch. The mechanical behaviour and biochemical reaction of the dermal fibroblasts were analysed. The stretched fibroblasts at day 5 showed the same mechanotransductive and biochemical features as unstretched myofibroblasts. Mechanical strain could induce the myofibroblasts differentiation and a cell model of HTS was established successfully at day 5. The expressions of lamin A/C, alpha‐smooth muscle actin, transforming growth factor beta 1, and collagen type I in fibroblasts were reduced by the silencing of Nesprin‐2. Mechanical strain could induce the myofibroblasts differentiation and silencing of Nesprin‐2 could block the mechanical stimulation of terminal myofibroblasts differentiation. Nesprin‐2 might be a potential target to treat the HTS.     

基于多指标成分定量结合化学计量学评价不同产地和不同采收期桔梗质量

目的 采用多指标成分定量结合化学计量学方法对不同产地和不同采收期的桔梗Platycodon grandiflorum进行质量评价。方法 采用热浸法测定其醇溶性浸出物含量,应用超高效液相-电喷雾检测器（UPLC-CAD）法同时测定桔梗皂苷D、桔梗皂苷D₃、去芹糖桔梗皂苷D、桔梗皂苷E、去芹糖桔梗皂苷E 5个活性成分含量,揭示不同采收期桔梗化学成分含量动态积累规律。运用主成分分析法分别对不同生长年限、不同产地桔梗进行综合评价,单因素方差分析比较不同产地桔梗各指标成分含量。结果 以春季4月、秋季9～10月、三年生为桔梗的最佳采收期。主成分分析、方差分析表明不同产地桔梗中指标含量存在显著性差异,同一产地不同批次间也有区别;根据主成分分析对不同产地桔梗进行综合评分,排名靠前的产地为甘肃陇南及河南省地区,陕西商洛、安徽亳州、内蒙古赤峰产地次之,山东淄博和山西太原产地排名较后。结论 以多指标成分定量结合化学计量学建立的评价方法明确了桔梗最佳采收期,评价了不同产地桔梗的质量,为桔梗药材质量控制研究提供依据。     

IL‐27 improves adoptive CD8+ T cells’ antitumor activity via enhancing cell survival and memory T cell differentiation

IL‐27 is an anti‐inflammatory cytokine that triggers enhanced antitumor immunity, particularly cytotoxic T lymphocyte responses. In the present study, we sought to develop IL‐27 into a therapeutic adjutant for adoptive T cell therapy using our well‐established models. We have found that IL‐27 directly improved the survival status and cytotoxicity of adoptive OT‐1 CD8⁺ T cells in vitro and in vivo. Meanwhile, IL‐27 treatment programs memory T cell differentiation in CD8⁺ T cells, characterized by upregulation of genes associated with T cell memory differentiation (T‐bet, Eomes, Blimp1, and Ly6C). Additionally, we engineered the adoptive OT‐1 CD8⁺ T cells to deliver IL‐27. In mice, the established tumors treated with OT‐1 CD8⁺ T‐IL‐27 were completely rejected, which demonstrated that IL‐27 delivered via tumor antigen–specific T cells enhances adoptive T cells’ cancer immunity. To our knowledge, this is the first application of CD8⁺ T cells as a vehicle to deliver IL‐27 to treat tumors. Thus, this study demonstrates IL‐27 is a feasible approach for enhancing CD8⁺ T cells’ antitumor immunity and can be used as a therapeutic adjutant for T cell adoptive transfer to treat cancer.     

The effectiveness of Salvianolate injection for in-stent restenosis after percutaneous coronary intervention: A protocol for systematic review and meta-analysis

Background:In-stent restenosis (ISR) caused by vascular remodeling after percutaneous coronary intervention limits the long-term efficacy of this method. Salvianolate injection is now widely used in the clinical treatment of ISR. However, there is no systematic review or meta-analysis to evaluate the effects of Salvianolate injection on ISR.Methods:We will search articles in 8 electronic databases, including the Cochrane Central Register of Controlled Trials, PubMed, Embase, the Web of Science, China National Knowledge Infrastructure, the Chinese Biomedical Literature Database, Wanfang Database, and the Chinese Scientific Journal Database for randomized controlled trials of ISR treated by Salvianolate injection from their inception to February 27, 2022. The primary outcome measure will be the restenosis rate. The data meeting the inclusion criteria were analyzed by RevMan V.5.4 software. Two authors evaluated the study using the Cochrane collaborative risk bias tool. We will use a scoring method to assess the overall evidence supporting the main results.Results:This study will analyze the clinical effectiveness of Salvianolate injection in the treatment of ISR.Conclusion:The findings of this systematic review will provide evidence to evaluate the effectiveness of Salvianolate injection for the treatment of ISR.INPLASY registration number:INPLASY202220117.     

Preparation and photodynamic antibacterial/anticancer effects of ultralong-lifetime room-temperature phosphorescent N-doped carbon dots

Room-temperature phosphorescent (RTP) N-doped carbon-dots (CNDs) featuring eco-friendliness, low cost and high biocompatibility, are ideal photodynamic antibacterial and anticancer nanomaterials. However, the existing CNDs are limited by low singlet oxygen (¹O₂) quantum yield, which has become a bottleneck in the development of CNDs. One basic reason is the short T₁-state exciton lifetime of CNDs. Herein, triethylenetetramine hexaacetic acid was used to synthesize CNDs via a one-step hydrothermal method. CNDs are characterized with low toxicity, high biocompatibility and ultralong-lifetime RTP (URTP). In addition to the URTP (average lifetime 414 ms) under solid conditions, CNDs even had URTP (average lifetime 320 ms) in a water environment. The ultralong T₁ exciton lifetime largely extends the collision time between T₁ state excitons and O₂ and prolongs the energy transfer time, not only improving the quantum yield (0.63) of singlet oxygen (¹O₂) in solution, but also facilitating the photodynamic antibacterial and anticancer effects.

The URTP CNDs largely extends the collision time between T₁ state exciton and O₂, which improve quantum yield of singlet oxygen (¹O₂) in solutions, facilitating the photodynamic antibacterial and anticancer effects.     

淫羊藿甙对OVX大鼠成骨细胞Smad4 mRNA的作用

目的：探讨淫羊藿甙（icariin）抑制骨质疏松的细胞内信号机制。方法：36只雌性Wistar大鼠随机分成3组：OVX（双侧卵巢切除）；OVX-I （OVX并注射icariin）；OVX-E（OVX并注射雌二醇）。术后4，6，8周时检测血清碱性磷酸酶（AIJP）的含量，并于术后8周时测体质量，取股骨干分离培养成骨细胞，用RT-PCR检测各组成骨细胞Smad4的mRNA含量。结果：（1）Icariin可以控制OVX大鼠体质量增加。（2）术后4，6周OVX-I和OVX-E组血清ALP值升高差异无统计学意义；OVX组ALP升高值与其他2组间差异有统计学意义（P〈0．01）；术后8周OVX、OVX-I和OVX-E组血清ALP值依次降低，差异有统计学意义（P〈0．01）。（3）术后8周，RT-PCR显示OVX、OVX-I和OVX-E组Smad4的mRNA含量依次增高，以OVX-E组最多。结论：淫羊霍甙可抑制OVX大鼠血清ALP水平的升高，且可能通过提高Smad4 mRNA抑制OVX大鼠体质量的增加。     

sTREM-1在内毒素诱导急性肺损伤早期的作用及意义

目的观察sTREM-1在内毒素诱导急性肺损伤早期的作用及意义。方法建立小鼠内毒素肺损伤的模型,酶联免疫吸附法检测肺组织以及血浆中sTREM-1、抗炎因子IL-10以及其他促炎因子IL-1β、IL-6。结果 sTREM-1具有与抗炎因子IL-10相同的动力学改变(r=0.99,P<0.01),且血浆中IL-1β/sTREM-1、IL-6/sTREM-1分别与组织中相应的比值正相关(r>0.90,P<0.01;r>0.80,P<0.01),血浆中IL-1β/sTREM-1和IL-6/sTREM-1之间以及组织中相应的比值之间均呈正相关(r=0.80,P<0.01;r=0.80,P<0.01)。结论 sTREM-1作为抗炎因子在LPS诱导的ALI过程中发挥保护肺组织的作用,而且血浆内sTREM-1与其他促炎因子形成的促炎/抗炎比值的检测可用于肺组织内炎症反应状态的评价。