搭建体验式沟通平台提高大学生人际交往能力

在生活节奏不断加快，竞争激烈的当今社会，大学生群体中不同程度地存在人际交往困难，影响了各种适应社会需求和自我发展能力的培养。通过搭建体验式沟通平台，形成一种团结友爱、朝气蓬勃的氛围，将有利于大学生形成和发展健康的个性品质，提高大学生的人际交往能力。     

人胚肺细胞对人冠状病毒的敏感性

人冠状病毒72-62和73-50干燥毒种,用人胚器官和原代人胚肾细胞合管培养未能引起细胞病变,而将病毒液接种到人胚器官细胞或人胚肺传代细胞第一代就能引起明显的细胞病变。新传代病毒经电镜及中和试验鉴定确属人冠状病毒。实验结果证明人胚肺传代细胞比原代人胚肾细胞敏感,为进一步研究人冠状病毒提供了一株敏感的细胞。     

胃癌病人NK细胞活性变化机理的初步研究

应用~(51)Cr释放试验和效靶结合试验,同时测定了正常人和胃癌病人外周血NK细胞活性与靶结合细胞数(TBC),研究了正常人及病人血浆对NK细胞功能的影响。发现正常人NK细胞活性与靶结合细胞数之间的关系呈直线正相关,但在NK活性降低的病人中靶结合细胞数却无明显改变,二者无相关性,而病人的血浆对正常人外周血NK活性则有明显的抑制作用(P<0.05),且抑制率与胃癌人NK细胞活性存有着负相关的关系,提示胃癌病人血浆具有抑制NK细胞活性的物质。     

双炔失碳酯类似物的抗生育活性研究

本文报道八个双炔失碳酯类似物的小鼠抗早孕、抗着床试验结果,同时考查了生物活性与油水分布系数之间的关系,发现抗早孕活性与油水分布系数之间为线性相关。双炔失碳半琥珀酸酯的抗早孕效果优于已用于临床的双炔失碳丙酸酯。     

In vivo and in vitro anti-tumour response of selenium-protein polysaccharide extracted from rich selenium Agaricus blazei

In this study, the anti-tumour activity of selenium-protein polysaccharide (SPP), a water extract of the rich selenium Agaricus blazei, was tested both in vivo and in vitro. The results of in vivo experiments show that SPP at doses of 50 and 100 mg/kg inhibits proliferation of implanted Sarcoma 180 by 22 and 37.69%, respectively, and promotes lymphocyte transformation and natural killer (NK) cells activity in tumour bearing mice. During the in vitro experiment, we treated the tumour and non-tumour bearing mice with SPP, and prepared serum treated with SPP (Serum_SPP). The results show that Serum_SPP, whether from tumour or non-tumour bearing mice, significantly inhibits K562 cells proliferation and induces their apoptosis, and also significantly increases caspase-3 activity of K562 cells. However, the difference in anti-tumour activity of Serum_SPP between tumour and non-tumour bearing mice is significantly different (p<0.01). The results, according to the studies both in vivo and in vitro, imply that SPP extracted from rich selenium A. blazei can inhibit growth of implanted Sarcoma 180 and promote lymphocyte transformation and NK cells activity in vivo. Additionally, Serum_SPP can inhibit proliferation and cause apoptotic morphological changes and the fragmentation of internucleosomal DNA, and increase caspase-3 activity of K562 cells in vitro, which indicates that apoptosis of K562 cells induced by Serum_SPP may be related to up-regulation of caspase-3.     

江西省528例非何杰金氏恶性淋巴瘤回顾性病理分析

本文收集了我省十年(1974—1983)来诊断为恶性淋巴瘤的病例,按免疫功能分类复查了全部切片,最后确诊为非何杰金氏恶性淋巴瘤(NHL)528例,进行了分析。本组NHL在首发部位、类型分布等方面与国内外有所不同。本组NHL首发于淋巴结外的占64.02％,明显高于国内其它省、市,而滤泡型淋巴瘤则低于国内多数地区。T细胞淋巴瘤占14.84％,较国内、外均低。并提出提高制片质量和广泛开展及应用免疫学技术的重要性。     

Preparation of [125I-Tyr27,Leu5]beta h-endorphin and its use for crosslinking of opioid binding sites in human striatum and NG108-15 neuroblastoma-glioma cells.

A radioligand suitable for crosslinking studies to opioid receptors has been obtained by radioiodination and purification of the monoiodotyrosine-27 derivative of the synthetic human beta-endorphin (beta h-endorphin) analogue [5-leucine]beta h-endorphin. The derivative, [27-[125I]monoiodotyrosine,5-leucine]beta h-endorphin, was crosslinked to human striatal (caudate and putamen) and NG108-15 neuroblastoma-glioma cell membranes by using disuccinimidyl suberate. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis under reducing conditions revealed four specifically labeled bands at 68, 40, 30, and 25 kDa for both human caudate and putamen, whereas NG108-15 cell membranes gave specifically labeled bands at 92, 56, 38, and 23 kDa.     

原发性甲状腺机能亢进症的手术探查技巧

目的探讨原发性甲状腺机能亢进症的手术探查技巧和经验教训。方法对50例原发性甲状腺机能亢进症患者临床资料进行回顾性总结分析。结果48例患者手术治疗，术后38例诊断为甲状旁腺瘤，6例为甲状旁腺增生，4例为甲状旁腺癌。48例中44例第一次手术探查成功（占88％），2例行2次手术，2例行3次手术。46例术后痊愈，2例并发多处肋骨骨折发生呼吸衰竭而死亡。结论确诊为原发性甲状腺机能亢进症伴有临床征象者均应手术治疗。术前病灶定位明确者手术较容易，定位不明确者应在“热区内”重点探查。