全文获取类型
收费全文 | 176349篇 |
免费 | 14753篇 |
国内免费 | 11526篇 |
专业分类
耳鼻咽喉 | 1509篇 |
儿科学 | 2107篇 |
妇产科学 | 2836篇 |
基础医学 | 20012篇 |
口腔科学 | 2932篇 |
临床医学 | 23540篇 |
内科学 | 27209篇 |
皮肤病学 | 1965篇 |
神经病学 | 9051篇 |
特种医学 | 5746篇 |
外国民族医学 | 95篇 |
外科学 | 17417篇 |
综合类 | 29182篇 |
现状与发展 | 34篇 |
一般理论 | 14篇 |
预防医学 | 11803篇 |
眼科学 | 4822篇 |
药学 | 18373篇 |
162篇 | |
中国医学 | 8896篇 |
肿瘤学 | 14923篇 |
出版年
2024年 | 476篇 |
2023年 | 2463篇 |
2022年 | 6454篇 |
2021年 | 8220篇 |
2020年 | 6093篇 |
2019年 | 5484篇 |
2018年 | 5642篇 |
2017年 | 5244篇 |
2016年 | 4885篇 |
2015年 | 7464篇 |
2014年 | 9471篇 |
2013年 | 8834篇 |
2012年 | 12819篇 |
2011年 | 14165篇 |
2010年 | 9115篇 |
2009年 | 7274篇 |
2008年 | 9449篇 |
2007年 | 9435篇 |
2006年 | 9377篇 |
2005年 | 8959篇 |
2004年 | 6090篇 |
2003年 | 5734篇 |
2002年 | 4885篇 |
2001年 | 4302篇 |
2000年 | 4320篇 |
1999年 | 4441篇 |
1998年 | 2872篇 |
1997年 | 2848篇 |
1996年 | 2020篇 |
1995年 | 1901篇 |
1994年 | 1636篇 |
1993年 | 1045篇 |
1992年 | 1488篇 |
1991年 | 1292篇 |
1990年 | 1117篇 |
1989年 | 959篇 |
1988年 | 864篇 |
1987年 | 800篇 |
1986年 | 602篇 |
1985年 | 540篇 |
1984年 | 299篇 |
1983年 | 207篇 |
1982年 | 138篇 |
1981年 | 136篇 |
1980年 | 86篇 |
1979年 | 135篇 |
1978年 | 59篇 |
1976年 | 52篇 |
1975年 | 55篇 |
1974年 | 65篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
Yi-ting Cai Cheng-liang Xiong Tian-shu Liu Shi-liang Shen Jin-peng Rao Feng Qiu 《Andrologia》2021,53(8):e14144
This study aimed at the efficacy of sequential treatment of bone marrow-derived mesenchymal stem cell secretion for busulfan-treated azoospermia in mice. The conditioned media (CM) was obtained from bone marrow mesenchymal stem cells (MSCs) or 293 cells. Chemically induced azoospermia mice received 200 μl MSC-CM or 293-CM twice a week intravenously for three consecutive weeks. The histological assessment of spermatogenic recovery quantifying the expression of meiosis-associated genes, and Sertoli cell barrier functional factors were assessed. The characteristics of TM4 cells (Sertoli cell line) after pre-incubation of MSC-CM in vitro were also obtained. The MSC-CM group had the most spermatogenic colonies among the three groups (p < .05), but no spermatids were seen. Expressions of the meiosis-associated genes Dazl, Vasa, Miwi, Stra8, CyclinA1, Pgk2 and Scp3 in MSC-CM testis were remarkably higher compared with 293-CM and busulfan groups respectively (p < .05). The levels of Sertoli cell barrier functional factors, for example ICAM-1 and N-cadherin, were significantly increased during MSC-CM treatment (p < .05). Moreover, pre-incubation of MSC-CM particularly accelerated the CD54 (ICAM-1) and CD44 expressions of TM4 cells and promoted cell inherent adhesion. MSC-CM treatment can significantly improve the short-term restoration of spermatogonial structures of chemically induced azoospermia related to facilitating Sertoli cell adhesion integrity. 相似文献
992.
Litong Wu Ying Chen Minjing Chen Yueqin Yang Zuzhao Che Qixin Li Xujun You Wei Fu 《Andrologia》2021,53(9):e14165
The present study aimed to investigate the molecular mechanism of the Astragalus–Scorpion drug pair in the treatment of prostate cancer (PCa). We employed network pharmacology and molecular docking technology to retrieving the active ingredients and corresponding targets of Astragalus–Scorpion by using TCMSP, BATMAN-TCM, TCMID and Swiss Target Prediction Databases. The targets related to PCa were retrieved through GeneCards. Cytoscape software was used to construct the ‘active ingredient–target disease’ network, and GO and KEGG enrichment analyses were performed on the common targets. Autodock software was used for molecular docking verification. In total, 26 active ingredients, 340 potential targets related to active ingredients and 122 common targets were screened from Astragalus–Scorpion drug pair. The core targets of the protein–protein interaction (PPI) network were JUN, AKT1, IL6, MAPK1 and RELA, whereas the core active ingredients were quercetin, kaempferol, formononetin, 7-o-methylisomucronulatol and calycosin. Nearly 762 GO entries and 154 pathways were obtained by using the pathway enrichment analysis. Molecular docking results revealed that quercetin and kaempferol bind to AKT1 and formononetin binds to RELA, all of which were found to be stable bounds. 相似文献
993.
The therapeutic effect of Interleukin-18 on hypertrophic scar through inducing Fas ligand expression
Xin Le Wei-Wei Wu 《Burns : journal of the International Society for Burn Injuries》2021,47(2):430-438
ObjectiveAmong downstream interleukin-18 (IL-18) targets, Fas ligand (FasL) in particular, has been strongly implicated in many conditions. Our study aims to explore the role of IL-18 in hypertrophic scar through enhancing FasL expression.MethodsIL-18 expression in hypertrophic scar tissues and normal tissues were explored by immunohistochemistry, qRT-PCR and Western blotting, and the expression of IL-18 in normal skin fibroblasts and hypertrophic scar fibroblasts by immunofluorescence. Hypertrophic scar fibroblasts treated with recombinant human IL-18 (rhIL-18) were assessed with MTT, Annexin V-FITC/PI, qRT-PCR, ELISA and western blotting. In the hypertrophic scar of rabbit ears, rhIL-18 was injected to determine histological changes with HE and Masson staining. Additionally, the scars were rated based on contour and overall severity using a visual analog scale scores (VAS).ResultsIL-18 was decreased in hypertrophic scar tissues and fibroblasts compared to normal skin tissues and fibroblasts, respectively. Decreased proliferation and increased apoptosis of hypertrophic scar fibroblasts were found after rhIL-18 treatment with enhanced expression of FasL, sFasL FADD, Caspase-8, Caspase-9 and Caspase-3 in a dose-dependent manner. The VAS and thickness of scars in rabbit ears was decreased as time went on after rhIL-18 treatment, with decreases in scar elevation index (SEI) and the increases in FasL expression.ConclusionIL-18 curbs proliferation and promotes apoptosis of hypertrophic scar fibroblasts by enhancing FasL expression. IL-18is a potential target for treatment of hypertrophic scar. 相似文献
994.
995.
996.
BackgroundUnicompartmental knee arthroplasty (UKA) has been proven to be an effective surgical technique for unilateral compartment osteoarthritis. The purpose of this study is to identify and analyze the top 100 cited articles in the field of UKA research.MethodsPublications on UKA from 1980 to 2020 in the Web of Science database were retrieved. The characteristics of the top 100 cited articles were analyzed, including information of publications and citations, level of evidence, and research interests.ResultsThe number of publications and citations increased over time. The majority of the highly cited articles were from the Nuffield Orthopedic Centre (Oxford, England) and the Brigham and Women’s Hospital (Boston, USA). Long-term outcome of UKA and comparison between UKA and TKA gathered most research interests. The most frequently occurring keywords were “survival” and “revision.” Since 2012, “life quality” and “robotics” have been used. There was no level I evidence, and most studies provided level IV evidence.ConclusionThere was a rising trend in publications and citations in the field of UKA research, the majority of them were from a few centers, and were low-level evidence. Most studies focused on the long-term outcomes of UKA; in recent years, patient satisfaction and navigation surgery have become new research trends. 相似文献
997.
Sean Z. Griffiths Mohamed F. Albana Lauryn D. Bianco Manuel C. Pontes Eddie S. Wu 《The Journal of arthroplasty》2021,36(3):946-952
BackgroundThe use of robotic-assisted total knee arthroplasty (TKA) has significantly increased over the past decade. Internet content is largely unregulated and may contain inaccurate and/or misleading information about robotic TKA. Our goal was to assess the content, quality, and readability of online material regarding robotic-assisted TKA.MethodsWe conducted an internet search for the top 50 web sites from each of the 3 most popular search engines (Google, Yahoo, and Bing) using the search term robotic total knee replacement. Each web site was assessed for content, quality, and readability. Web site quality was assessed utilizing the QUality Evaluation Scoring Tool (QUEST). Readability was assessed utilizing the Simple Measure of Gobbledygook, Flesch-Kincaid Grade Level, and Flesch Reading Ease Formula scores.ResultsGeneral risks of TKA were discussed in 47.2%, while benefits were discussed in 98.6% of all web sites. Inaccurate claims occurred at a significantly higher rate in physician/community hospital sources compared to university/academic web sites (59% vs 28%, P = .045). Web sites from university/academic web sites had the highest QUEST scores, while physician/community hospital sources scored the lowest (16.1 vs 10.6, P = .01). Most web sites were written at a college reading level or higher.ConclusionPatients should be counseled on the largely unregulated nature of online information regarding robotic-assisted TKA. Physicians and hospitals should consider revising the readability of their online information to a more appropriate level in order to provide accurate, evidence-based information to allow the patient to make an informed consent decision. 相似文献
998.
Rui Sheng Yuan Wang Yunshu Wu Jun Wang Shiwen Zhang Qiwen Li Danting Zhang Xingying Qi Qingyue Xiao Shuang Jiang Quan Yuan 《Journal of bone and mineral research》2021,36(2):412-423
N6-methyladenosine (m6A), as a eukaryotic mRNA modification catalyzed by methyltransferase METTL3, is involved in various processes of development or diseases via regulating RNA metabolism. However, the effect of METTL3-mediated m6A modification in tooth development has remained elusive. Here we show that METTL3 is prevalently expressed in odontoblasts, dental pulp cells, dental follicle cells, and epithelial cells in Hertwig's epithelial root sheath during tooth root formation. Depletion of METTL3 in human dental pulp cells (hDPCs) impairs proliferation, migration, and odontogenic differentiation. Furthermore, conditional knockout of Mettl3 in Osterix-expressing cells leads to short molar roots and thinner root dentin featured by decreased secretion of pre-dentin matrix and formation of the odontoblast process. Mechanistically, loss of METTL3 cripples the translational efficiency of the key root-forming regulator nuclear factor I-C (NFIC). The odontogenic capacity of METTL3-silenced hDPCs is partially rescued via overexpressing NFIC. Our findings suggest that m6A methyltransferase METTL3 is crucial for tooth root development, uncovering a novel epigenetic mechanism in tooth root formation. © 2020 American Society for Bone and Mineral Research (ASBMR). 相似文献
999.
Immune and repair responses in joint tissues and lymph nodes after knee arthroplasty surgery in mice
1000.
Saqib Masroor Qiong Qiu Mohamad Alghothani Rajesh Gupta George V. Moukarbel 《Journal of cardiac surgery》2021,36(1):398-400
Percutaneous occlusion of the left atrial appendage is increasingly being used as an alternative for stroke prevention in patients with non‐valvular atrial fibrillation at high risk of complications from long term anticoagulation. We describe a case of left atrial appendage perforation during Watchman device implantation requiring emergency repair of the left atrium using sternotomy and cardiopulmonary bypass. Technical considerations for surgical decision making are discussed; in hemodynamically unstable patients as well as those at high risk for embolization. 相似文献