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991.
Wu AH Holtman V Apple FS Ricchiuti V DiBello PM Jacobsen D 《Annals of clinical and laboratory science》2000,30(2):185-190
A fully automated immunoassay for total plasma homocysteine assay was evaluated at four centers. To measure total homocysteine, oxidized forms of homocysteine in serum and plasma were reduced by dithiothreitol and assayed by a competitive fluorescence polarization technique. The assay had within-run precision from 0.9 to 3.0% and total precision from 2.8 to 4.1% for control materials with homocysteine concentrations of approximately 7, 12.5, and 25 micromol/L, a sensitivity of 0.35 micromol/L, good parallelism upon dilution, and analytical recovery ranging from 97.4 to 103.8%. The immunoassay correlated with four different HPLC assays for homocysteine, yielding a slope of 0.98, an intercept of -0.19 micromol/L, and a correlation coefficient of 0.966 for 440 paired samples. The reference range, determined with plasma samples from 609 males and 600 females, yielded a mean of 9.17+/-2.86 micromol/L, with a central 95% range of 4.78-15.43 micromol/L. The immunoassay is a suitable alternative to HPLC and may be useful in screening persons with high risk of coronary artery disease. 相似文献
992.
In order to clarify morphology and function(s) of the terminal nerve, gross examinations of the nerve were carried out in 9 species of selachians. The terminal ganglion was observed light- and electron-microscopically. FMRF-amide immunoreactivity of the terminal nerve was also examined in some species. The results were as follows: 1) The terminal nerve was divided into peripheral and central branches by interposed ganglion(s). Macroscopically, the peripheral branch appeared from an area between the nasal sac and the olfactory bulb. The central branch entered the telencephalon from either the rostral, dorsal, or ventral surface. The position seemed to differ from species to species. 2) The terminal nerve showed great species differences and individual variations in the macroscopic morphology, such as number and course of the peripheral branch, position, size and number of ganglia, and telencephalic areas where the central branch entered. Even in the same individual, there was a difference in left and right sides. 3) According to general histology, the ganglion was encapsulated and had no direct connection with the telencephalon through the capsule, even when the ganglion was situated on the telencephalon. The Bodian preparations showed that most ganglion cells were unipolar, and a few were bipolar or pseudounipolar. 4) All ganglion cells and the processes were FMRF-amide immunoreactive. Immunoreactive fibers of the central branch terminated in the septal and preoptic areas. FMRF-amide immunoreactive cells were also found in the olfactory nerves or the septal area of the telencephalon in some species. These neurons were thought to be ectopic ganglion cells. 5) Neuronal somata and the axons in the ganglion contained large dense cored vesicles.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
993.
The number of sodium pump sites on erythrocytes was measured on 1,847 individuals in 80 Utah kindreds ascertained through probands with cardiovascular disease. Likelihood analysis supported recessive inheritance of high pump number. The major locus explained 14.0% of the variance in pump number; polygenic inheritance explained another 63.4%. Homozygotes for the recessive allele occurred with a frequency of 1.74% and had a mean pump number estimated as 566.0 sites/red blood cell (RBC) versus a mean of 312.2 sites/RBC for the other genotypes. Young individuals with the high pump number genotype were leaner, and older adults with the high pump number genotype were heavier. Diabetes and early hypertension were more prevalent in women with the high pump number genotype. Although not significant, obesity in adults of both sexes and early coronary heart disease in men were more prevalent in individuals with the high pump number genotype. 相似文献
994.
Syndiotactic polystyrene was efficiently prepared in the presence of a homogeneous catalyst composed of (η5-cyclopentadienyl)tribenzyloxytitanium and methylaluminoxane (MAO) as a cocatalyst. Influences of various polymerization conditions, e.g., [Ti]. [MAO], [St], temperature and the content of retained trimethylaluminium (TMA) in MAO on the catalytic activity, syndiotacticity and molecular weight of the polymer were studied. It was found that the retained TMA plays an important role in the polymerization. Lowering the retained TMA content in MAO decreases the activity of the catalyst remarkably. Addition of external alkylaluminium (TMA or triisobutylaluminium) into the catalyst system with MAO containing low amounts of retained TMA promotes styrene polymerization. 相似文献
995.
用体外琼脂培养法研究了PHA对小鼠骨髓CFU-GM的影响,结果表明,小鼠经腹腔注射PHA后,对小鼠CFU-GM产率和自杀率的影响随PHA的剂量不同而异,20~40mg/kg的PHA能明显提高CFU-GM的产率,其作用时间维持3天,剂量大于50mg/kg,可抑制CFU-GM产率。PHA组自杀率较对照组明显提高,提示适量的PHA在体内可促进小鼠CFU-GM的增殖,使更多的CFU-GM由Go期进入S期。将PHA直接加入无CSF的培养体系中,未见集落生长。此项结果提示PHA可能通过间接机制而非直接作用于CFU-GM。 相似文献
996.
The open reading frame 3 (ORF3) of the severe acute respiratory syndrome coronavirus (SARS-CoV) genome encodes a predicted 154-amino acid protein, which lacks similarities to any known protein, and is named 3b. In this study, it was shown that 3b protein was predominately localized to nucleus with EGFP tag at its N- or C-terminus. The localization patterns were similar in different transfected cells. Immuno-fluorescence assay revealed that 3b protein was co-localized well with C23 in nucleolus. C23, B23 and fibrillarin all are important nucleolar proteins, which localize in the region of the nucleolus. Co-transfection of p3b-EGFP with pC23-DsRed, pB23-DsRed and pfibrillarin-DsRed further confirmed 3b's nucleolus localization. With construction of serial truncated mutants of 3b, a region (residues 134-154 aa) responsible for nucleolar localization was determinated in 3b protein. These results provide a new insight for further functional studies of SARS-CoV 3b protein. 相似文献
997.
目的 定位一个双眼多形性先天性白内障家系的致病位点,寻找疾病家系的致病基因.方法 应用ABI-MD10试剂盒中的常染色体382个微卫星位点,对一个常染色体显性、双眼具有不同白内障形态的先天性白内障家系进行全基因组扫描.MLINK软件进行两点连锁分析.直接测序候选基因外显子.结果 在D12S83处获得最大LOD值Zmax=5.44(θ=0),该家系致病位点被定位到12p11.2-q15之间的区域上,在该区域的白内障候选基因晶状体膜蛋白基因(major intrinsic protein,MIP)的第4外显子中发现单个碱基错义突变,702ntG→A,导致p.R233K.结论 该先天性白内障由MIP基因702ntG→A突变所致,导致MIP蛋白质的C-末端中p.R233K,从而可能减少了氢键的形成,影响了该蛋白C-末端的稳定性,推测影响了MIP对水的调节及由该蛋白质形成的细胞之间的连接.这一新突变为目前世界上首次报道. 相似文献
998.
磁性阿霉素纳米微球的制备及在高频磁场中的发热研究 总被引:3,自引:0,他引:3
制备一种在高频磁场中能感应发热的用于治疗肿瘤的阿霉素纳米微球,研究其在磁场中的热效应。用超声搅拌冷冻干燥的方法制备药物微球,平均粒径200nm左右。电镜观察其形态为球囊状。将其置于不同介质中于高频磁场中测其温度变化值,实验表明该微球在交变磁场中使介质升温。升温速度与平稳时的温度和微球的量及磁场强度成正比,介质流动性好,升温快。 相似文献
999.
Current trends in the management of type 2 diabetes mellitus, based on the 20-year United Kingdom Prospective Diabetic Study, include intensive treatment to control the blood glucose level and blood pressure in order to prevent or delay microvascular and cardiovascular complications. In the new millennium, type 2 diabetes will become epidemic in developing countries. If diabetes were to develop in 10% of the 1.2 billion population of China, the expense of intensive treatment would be immense. Laboratory tests are useful for detecting risk factors before the onset of the disease and convincing the general public to take preventive measures. Glucose tolerance testing is one of these tests. When glucose tolerance is impaired, 25% of beta-cell function is lost. Determining the plasma proinsulin level is another useful evaluation; impaired glucose tolerance accompanied by increased plasma proinsulin level is indicative of an enhanced risk that type 2 diabetes will develop within 5 years. Educating the public about eating a healthy diet and exercising may prevent the development of diabetes and thereby reduce the global prevalence of type 2 diabetes. 相似文献
1000.
我们以前曾报道,表达单纯疱疹病毒Ⅱ型糖蛋白D(HSV-2gD)的重组痘苗病毒(实验疫苗株)能保护被免疫小鼠抵抗致死量HSV-2病毒的攻击。在此工作基础上,严格按人用疫苗研究要求的实验条件,成功地建立了表达HSV-2gD的重组痘苗病毒活疫苗株。首先将经聚合酶链反应(PCR)修饰的HSV-2gD基因插入痘苗表达质粒pJSB1175,置于痘苗病毒P75K早/晚期启动子控制下。将此重组质粒用Lipofectin方法转染已受野型TK+痘苗病毒天坛761株感染的人胚肺二倍体细胞。经同位素探针(32P-HSV-2gD)原位杂交法和3轮蚀斑纯化,筛选出基因组内整合有HSV-2gD基因的重组痘苗病毒。斑点和Southern杂交证实,HSV-2gD基因已插入痘苗病毒基因组内预期的TK区段,间接免疫荧光检测显示,重组病毒感染细胞后能有效地表达HSV-2gD蛋白。 相似文献