Meta-analysis of human lung cancer microRNA expression profiling studies comparing cancer tissues with normal tissues

ABSTRACT: BACKGROUND: Lung cancer is the major cause of cancer death globally, it is often diagnosed at an advanced stage and has one of the lowest survival rates of any type of cancer. The common interest in the field of lung cancer research is the identification of biomarkers for early diagnosis and accurate prognosis. There is increasing evidence to suggest that microRNAs play important and complex roles in lung cancer. METHODS: A meta-analysis was conducted to review the published microRNA expression profiling studies that compared the microRNAs expression profiles in lung cancer tissues with those in normal lung tissues. A vote-counting strategy that considers the total number of studies reporting its differential expression, the total number of tissue samples used in the studies and the average fold change was employed. RESULTS: A total of 184 differentially expressed microRNAs were reported in the fourteen microRNA expression profiling studies that compared lung cancer tissues with normal tissues, with 61 microRNAs were reported in at least two studies. In the panel of consistently reported up-regulated microRNAs, miR-210 was reported in nine studies and miR-21 was reported in seven studies. In the consistently reported down-regulated microRNAs, miR-126 was reported in ten studies and miR-30a was reported in eight studies. Four up-regulated microRNAs (miR-210, miR-21. miR-31 and miR-182) and two down-regulated mcroiRNAs (miR-126 and miR-145) were consistently reported both in squamous carcinoma and adenocarcinoma-based subgroup analysis, with the other 14 microRNAs solely reported in one or the other subset. CONCLUSIONS: In conclusion, the top two most consistently reported up-regulated microRNAs were miR-210 and miR-21. The results of this meta-analysis of human lung cancer microRNA expression profiling studies might provide some clues of the potential biomarkers in lung cancer. Further mechanistic and external validation studies are needed for their clinical significance and role in the development of lung cancer.     

NRG1在肝细胞肝癌中杂合性丢失和蛋白表达的研究

目的：分析在肝细胞性肝癌中NRG1基因的两个多态性位点D8S278和D8S499杂合性丢失（loss ofheterozygosity,LOH）情况及其与NRG1蛋白表达水平之间的关系,探讨NRG1在肝细胞肝癌发生发展中的作用。方法：酚氯仿抽提经石蜡包埋的肝癌和癌旁正常组织DNA,进行PCR扩增,扩增出来的产物进行变性聚丙烯酰胺凝胶电泳并分析两位点杂合性丢失情况;用免疫组织化学方法检测肝癌组织和癌旁正常组织中NRG1的表达情况。结果：D8S278和D8S499的LOH率分别为32.43%（24/74）、37.86%（26/69）,总频率为48.65%（36/74）;NRG1在肝癌组织中的阳性表达率为67.57%（50/74）,显著低于在正常组织中的表达水平（97.30%,72/74）（P〈0.05）,这种降低与NRG1基因多态性位点的LOH有关;NRG1表达下调与肿瘤大小、Edmondson分级相关,而与年龄、性别、HBsAg、AFP、肝硬化和肝内外转移无明显相关。结论：NRG1基因可能是肝细胞肝癌的一个候选抑癌基因,在肝癌发生发展中起一定的作用;可为临床诊断、治疗及预后评估提供理论依据。     

薏仁多糖诱导人肺癌A549细胞凋亡

背景与目的薏仁是禾木科植物薏米的干燥成熟种仁,是常用的中药,又是普遍、常吃的食物。本研究旨在探讨薏仁多糖诱导A549细胞凋亡的作用。方法本研究通过水提醇沉法提取粗的薏仁多糖,再通过透析、离子交换柱层析等方法得到薏仁多糖组份(CP-1)。采用MTT测定A549细胞的存活率;扫描电镜观察细胞形态;流式细胞仪检测细胞周期;RT-PCR测定caspase-3和caspase-9两种基因相对表达量;单细胞凝胶电泳检测细胞DNA变化。结果M测得薏仁多糖对A549细胞存活率有明显的生长抑制作用。扫描电子显微镜观察到测试组A549细胞产生凋亡小体,流式细胞仪分析薏仁多糖处理导致A549细胞S期阻滞,并产生凋亡峰。RT-PCR结果表明与对照组比较,测试组caspase-3和caspase-9表达量上升。通过DNA彗星实验薏仁多糖可延长尾距,表明DNA断裂破碎。结论研究结果表明薏仁多糖组分可有效地诱发A549癌细胞凋亡。     

康莱特注射液联合顺铂治疗肺癌的疗效观察

目的:观察康莱特注射液联合顺铂治疗肺癌的疗效,并探讨其安全性。方法:选择2013年6月~2014年6月本院收治的肺癌患者80例随机分为两组,每组40例,对照组采用吉西他滨注射液联合顺铂注射液治疗,观察组在对照组的基础上应用康莱特注射液治疗,均以21 d为1个周期,2个周期为1个疗程,比较两组患者治疗3个疗程后的疗效。结果:观察组总有效率65.0%,明显高于对照组的30.0%,χ2=9.637,P0.05;观察组Karnofsky评分有效率67.5%,明显高于对照组的32.5%,P0.05;观察组不良反应发生率为17.5%,显著低于对照组的42.5%,统计学差异显著,χ2=4.463,P0.05。结论:康莱特注射液联合顺铂治疗肺癌疗效佳,不良反应少,临床应用较为安全,具有重要的临床价值。     

中间件在医院信息系统中的应用

目的解决各个系统之间的互通,实现医院内部系统之间资源共享。方法采用消息中间件技术集成应用,充分利用现有的医疗信息系统资源,进行现有异构信息系统的集成。结果利用消息中间件设计了HIS与RIS集成的设计方案、结构体系和流程。结论基于中间件技术的集成在临床应用中运行良好、易维护、通用性好,提高了医疗质量和临床工作效率,体现了一定的功能优势。     

体重体成分与骨密度的关系

为了研究体成分与骨密度（ＢＭＤ）之间的关系，因体重与ＢＭＤ显著相关，体成分各个组成相加等于体重，而体成分与ＢＭＤ的关系仍不清楚。方法随机选取２０６名１６～５２岁健康的男女性汉族人，用双能Ｘ射线吸收法（ＤＸＡ）测量ＢＭＤ与体成分，进行ＢＭＤ与体重，体成分的多元线性回归分析。结果体重，瘦组织（ＬＴＭ）与男女性的ＢＭＤ显著正相关，脂肪组织（ＦＴＭ）仅对女性全身，腰椎ＢＭＤ起显著性作用。结论影响男女性ＢＭＤ的体成分中，ＬＴＭ是主要因素，ＦＴＭ仅对女性ＢＭＤ有影响。本文较全面地研究了体成分与ＢＭＤ的关系。     

德尔格Evita 4呼吸机气路分析及应用

目的：通过对德尔格Evita4呼吸机气路的分析与应用维护的探讨,获得保证设备安全稳定运行的经验。方法：结合实际工作,重点分析Evita4呼吸机气路构成及工作原理,建立科学的设备日常维护方法。结果：分析呼吸机内部气路,确定设备维护方案,保证设备使用安全和应用质量。结论：通过分析气路,指导临床工程师进行应用维护,保证设备高效运行。     

关于医疗卫生体制改革的认知差异性

卫生改革的顺利实施依赖于卫生系统内不同群体的合作,但是现有的文献表明,不同群体对于医疗卫生体制改革的认知存在较为明显的差异,影响卫生政策的实践效果.通过对文献的分析,对差异的程度进行定性分析;对认知差异的定量研究是未来研究需关注的领域.