CLINICAL ANALYSIS OF FOUR CHINESE HEMOPHILIACS WITH HUMAN IMMUNODEFICIENCY VIRUS INFECTION

Human immunodeficiency virus (HIV) is now considered as the causative agent of acquired immunodeficiency syndrome (AIDS). A high risk of AIDS has been reported among patients with hemophilia who received lyophilized commercial factor VIII and IX concentrates of American origin. At a prevalent survey from September to December 1985, HIV antibodies were found in all four patients with hemophilia treated with the batch number W87307, 955 I.U. of American commercial factor VIII concentrate supplied by Armour Pharmaceutical Company, USA. One of the sero-positive patients developed AIDS-related complex (ARC) and died of cerebral hemorrhage. The other three sero-positive patients had abnormalities in cell-mediated immunity. Of them two developed left lumbosacral radiculopathy and hemorrhagic herpes zoster and one remained well so far.     

Clinical features of human intestinal capillariasis in Taiwan

Human intestinal capillariasis is a rare parasitosis that wasfirst recognized in the Philippines in the 1960 s.Parasitosisis a life threatening disease and has been reported fromThailand,Japan,South of Taiwan (Kaoh-Siung),Korea,Iran,Egypt,Italy and Spain.Its clinical symptoms arecharacterized by chronic diarrhea,abdominal pain,borborygmus,marked weight loss,protein and electrolyteloss and cachexia.Capillariasis may be fatal if earlytreatment is not given.We reported 14 cases living in ruralareas of Taiwan.Three cases had histories of travelling toThailand.They might have been infected in Thailand whilestayed there.Two cases had the diet of raw freshwaterfish before.Three cases received emergency laparotomydue to peritonitis and two cases were found of enteritiscystica profunda.According to the route of transmission,freshwater and brackish-water fish may act as theintermediate host of the parasite.The most simple andconvenient method of diagnosing capillariasis is stoolexamination.Two cases were diagnosed by histology.Mebendazole or albendezole 200 mg orally twice a day for20-30 d is the treatment of choice.All the patients werecured,and relapses were not observed within 12 mo.     

Effect on peripheral nerve vivo with human insulin-like regeneration by transgene in growth factor-1

BACKGROUND: Human insulin-like growth factor (hIGF-1) has been successful in treating peripheral nerve injury, but it is still unclear whether hIGF-1 after transgene in vivo has the effect on promoting the regeneration of peripheral nerve. OBJECTIVE: To observe the effect of hIGF-1 on the regeneration of peripheral nerve by transgene in vivo with electrophysiology, histological morphology and ultromicro morphology. DESIGN: A univariate design. SETTINGS: Jilin Institute of Surgery, China-Japan Friendship Hospital Affiliated to Jilin University; School of Basic Medical Sciences, Jilin University. MATERIALS: Thirty male adult Wistar rats of grade Ⅱ, weighing 200-250 g, were provided by the Animal Experimental Center of Jilin University [certification number: SCXK-(Ji)20030001]. The rats were raised in the environment at the temperature of 25 ℃ and humidity of 70%. All the rats were randomly divided into hIGF-1-treated group, treatment control group and blank control group, 10 rats in each group. Positive liposomes (mass concentration of 2 g/L) and pcDNA3.1 (mass concentration of 1 g/L) were purchased from Beijing Yuanpinghao Company; pcDNAhIGF-1 (mass concentration of 1 g/L) was provided by Dr. Shen from the School of Public Health of Jilin University. The liposomes were mixed with plasmids with the mass ratio of 1.5 to 10.Operative microscope was made by Jiangsu Zhenjiang Microsurgical Instrument Factory; EMB-5304K electromyogram (EMG) evoked potential meter by Nihon Kohden Corporation. HPIAS-1 000 high-acuity color pathological imaging analytical system (Japan) and JEM-1200EX transmission electron microscope (Japan) were also used. METHODS: The experiments were carried out in Jilin Institute of Surgery from April to June in 2004. ① All the rats were anesthetized, and the right sciatic nerve was exposed, and it was clipped with a clip at 5 mm below the piriform muscle for 3 times, 10 s for each time. The pressed width was 3 mm, and formed as membrane under operating microscope (×6). Rats in the hIGF-1-treated group were subepineurially injected with the mixture of pcDNAhIGF-1 and positive liposomes (10 μL) immediately, those in the treatment control group were injected with the mixture of pcDNA3.1, positive liposomes and distilled water (10 μL), and those in the blank control group were not given any injection. ② The sciatic nerve functional indexes (SFI) were measured within 56 days postoperatively according to the methods used by Shen et al. ISFI=0 was taken as normal, and ISFI=-100 as completely damaged. EMG evoked potential meter was used to record the electrophysiological changes of the regenerated nerve fibers. The indexes of histological morphology in 5 randomly selected sights were determined with the color pathological imaging analytical system, and the ultrostructures of the regenerated nerve fibers were also observed. MAIN OUTCOME MEASURES: ① Comparison of the SFI within 56 days postoperatively; ② Comparison of the electrophysiology, histological morphology and ultrastructure of the regenerated nerve fibers 56 days postoperatively. RESULTS: All the 30 Wistar rats were involved in the analysis of results. ① SFI: The SFI values were gradually increased as time prolonged in all the three groups, and the changes were more obvious after 24 days, the SFI values recovered better at each time point in the hIGF-1-treated group than in the other two groups. ② Eelectrophysiological results of right sciatic nerve: The latency of motor evoked potential (MEP) was close between the treatment control group and the blank control group [(2.55±0.36), (2.65±0.55) ms, P > 0.05], but higher in the hIGF-1-treated group [(2.14±0.22) ms] than in the blank control group (P < 0.01). The amplitude and conduction velocity of MEP in the treatment control group [(6.67±0.69) mV, (29.57±4.06) m/s] were close to those in the blank control group [(6.60±0.59) mV, (29.22±3.20) m/s, P > 0.05], but those in the hIGF-1-treated group [(7.81±0.84) mV, (36.91±4.37) m/s] were larger or faster than those in the blank control group (P < 0.01). ③ Results of the pathological image analysis of the regenerated nerve fibers: The axonal diameter, thickness of myelin sheath of the regenerated nerve fiber and the number of myelinated nerve fiber in the treatment control group [(2.28±0.33) μm, (1.08±0.18) μm2, (71.80±8.25) fibers] were close to those in the blank control group [(2.18±0.29) μm, (1.03±0.15) μm2, (68.60±8.55) fibers] (P > 0.05), and those in the hIGF-1-treated group [(3.03±0.35) μm, (1.65±0.24) μm2, (88.20±8.82) fibers] were obviously larger or more than those in the blank control group (P < 0.01). ④ Ultrastructure of the regenerated nerve fibers of sciatic nerve: In the hIGF-1-treated group, the regenerated fibers of sciatic nerve were more and mature, manifested by thicker nerve fibers, thicker and evener myelin sheath, which were better than those in the other two groups. CONCLUSION: The results of the quantitative parameters of the electrophysiology, gross histological morphology and ultrostructural changes in the process of repairing damaged peripheral nerve indicate that transgene in vivo with hIGF-1 can promote the neural regeneration after peripheral nerve injury.     

Effects of phycocyanin on apoptosis and expression of superoxide dismutase in cerebral ischemia reperfusion injury

IN T R O D U C T IO NSuperoxide dism utase (SO D )is an im portantendogenous free radica scavenger, itelim inates superoxide anion free radicals through dis proportionation, and itprotects the nerve cells in the process ofcere bralischem ia reperfusion in…     

用利多卡因和普鲁卡因配制的两种心脏停搏液对心肌保护效果的临床观察

本文用利多卡因和普鲁卡因配制的两种心脏停搏液对228例病人心内直视手术的心肌保护效果进行观察比较。两组心脏停搏液的基础成份:林格氏液400ml、20％甘露醇30ml;50％葡萄糖20ml,氯化钾0.5、硫酸镁0.1,ATP20mg、碳酸氢钠0.5,0～4℃。pH7.35～7.55,渗透压460～490mOsm/L。利多卡因组加利多卡因0.2,普鲁卡因组加普鲁卡因0.1。结果表明:利多卡因组心脏自动复跳102例(89.5％),明显高于普鲁卡因组73例(64.0％),且复跳后大多为窦性心律,无传导阻滞,需用药物支持心搏出量的9例(9.6％)少于普鲁卡因组的20例(17.5％)。经临床应用,我们认为利多卡因组用于心肌保护的效果优于普鲁卡因组。     

未破裂脑动脉瘤的治疗选择

未破裂脑动脉瘤的处理存在争议。由于其自然史尚未完全明确,因此最佳的治疗策略也不清楚。目前对未破裂脑动脉瘤处理的共识包括观察、显微手术夹闭和血管内治疗。用于随访已知未破裂脑动脉瘤的方法学也有争议,可能取决于经治医生的偏好。大多数动脉瘤由神经外科医师和介入神经放射科医师处理,但未破裂动脉瘤通常是由神经科医师在对患者进行其他神经系统疾病的筛查时首先发现的。因此,关于何时对患者进行筛查和如何对未破裂动脉瘤采取最佳处理的知识将对其日常医疗实践有直接的影响。未破裂动脉瘤经常导致包括缺血事件、癫和头痛在内的其他神经系统症状,这些症状可能促进更积极的干预治疗。由于缺乏设计完善真正基于人群的研究或随机试验,因此目前的最佳处理必须以现有文献和每例患者当时的具体情况为根据。     

肝硬化腹水并发原发性细菌性腹膜炎48例治疗体会

肝硬化腹水并发原发性细菌性腹膜炎４８例治疗体会闫玉伦，金世禄（滨州地区人民医院，２５６６１０）关键词肝硬化；肝硬化腹水；腹膜炎；细菌性腹膜炎肝硬化腹水患者易并发原发性细菌性腹膜炎（简称ＳＢＰ），使病情恶化，预后更差。我们近６年共收治肝硬化腹水并发ＳＢ...     

Therapeutic Effect of Berberine on 60 Patients with Non－Insulin Dependent Diabetes Mellitus and Experimental Research

ＴｈｅｒａｐｅｕｔｉｃＥｆｆｅｃｔｏｆＢｅｒｂｅｒｉｎｅｏｎ６０ＰａｔｉｅｎｔｓｗｉｔｈＮｏｎ－ＩｎｓｕｌｉｎＤｅｐｅｎｄｅｎｔＤｉａｂｅｔｅｓＭｅｌｌｉｔｕｓａｎｄＥｘｐｅｒｉｍｅｎｔａｌＲｅｓｅａｒｃｈＮｉＹａｎ－ｘｉａ（倪艳霞）；ＬｉｕＡｎ－ｑ...     

证候数据库录入的方法学探讨

古今医案及现代证候规范研究成果数据库的建立是“证候的规范及其与疾病、方剂相关的基础研究”中“证候规范与辨证方法体系的研究”课题的重要的基础性工作。在此项研究中,将对相关资料中涉及到的四诊信息与现代理化信息,还有来自社会和自然对证候有影响的信息全面采集,高度开     

THE INFLUENCE OF MULTIPLE FACTORS ON GALL BLADDER PRESSURE AFTER AURICULAR POINT STIMULATION

The study has explored 11 correlative variables which might affect the gallblad-der pressure GBP of patients with gall stone after auricular electrical stimulation by means of multiplestepwise regression. It was found that the size of gallbladder stone (X9), cholecystolithiasis (X4),sex (X1), and the baseline of GBP (X10) could affect the GBP change range (Y1) depressingly orreinforcingly. Also the baseline of GBP (Y1) could be affected by the variables X1 (sex), X2 (age),and X6 (long diameter of the gallbladder, LDGB), etc.. The result shows that the change in GBPafter auricular electrical stimulation is comprehensively influenced by multiple factors. And it providessome useful information for predicting the curative effect of auricular therapy on gallbladder stone inclinic.