维生素C和维生素E辅加剂降低引起胚胎毒性的氧化作用和提高胚囊发育率

维生素C和维生素E辅加剂降低引起胚胎毒性的氧化作用和提高胚囊发育率@Wang X @赵铀     

N-Phenylethyl Amitriptyline in Rat Sciatic Nerve Blockade

Background: The antidepressant amitriptyline is commonly used orally for the treatment of chronic pain, particularly neuropathic pain, which is thought to be caused by high-frequency ectopic discharge. Among its many properties, amitriptyline is a potent Na+ channel blocker in vitro, has local anesthetic properties in vivo, and confers additional blockade at high stimulus-discharge rates (use-dependent blockade). As with other drug modifications, adding a phenylethyl group to obtain a permanently charged quaternary ammonium derivative may improve these advantageous properties.

Methods: The electrophysiologic properties of N-phenylethyl amitriptyline were assessed in cultured neuronal GH3 cells with the whole cell mode of the patch clamp technique, and the therapeutic range and toxicity were evaluated in the rat sciatic nerve model.

Results: In vitro, N-phenylethyl amitriptyline at 10 [mu]m elicits a greater block of Na+ channels than amitriptyline (resting block of approximately 90%vs. approximately 15%). This derivative also retains the attribute of amitriptyline in evoking high-degree use-dependent blockade during repetitive pulses. In vivo, duration to full recovery of nociception in the sciatic nerve model was 1,932 +/- 72 min for N-phenylethyl amitriptyline at 2.5 mm (n = 7) versus 72 +/- 3 min for lidocaine at 37 mm (n = 4; mean +/- SEM). However, there was evidence of neurotoxicity at 5 mm.     

Study on therapeutic mechanism of Neiyifang in treating endometriosis

Objective: To observe the clinical effect of Neiyifang (NYF) in treating endometriosis and to explore its therapeutic mechanism through observing its influence on plasma β-endorphin (β-EP) in different menstrual stages and levels of prostaglandins (PGs) in menstruation.Methods:NYF was administered to 104 patients with endometriosis one dose daily with 3 successive menstrual cycles as one therapeutic course. Peripheral blood β-EP level in follicular, luteal and menstrual stages, as well as PGF_2α, PGE2, thromboxane B₂ and 6-keto-PGFl α levels in menstrual stage were detected by RIA, and controlled with those in 15 healthy persons.Results: (1) The total effective rate of NYF was 81.3% and it showed significant effect in improving patients’ clinical symptoms and physical signs; (2) In menstrual stage, the levels of β-EP, 6-keto-PGF_α/ TXB₂ were lower(P <0.05) and levels of PGF_2α, PGE₂, TXB₂ and 6-keto-PGF_1α were higher (P< 0. 05) in patients than those in control, and the higher the level of PGE₂, the severer the menalgia, (3) NYF could increase levels of β-EP, 6-keto-PGF_1α, and reduce levels of PGF_2α, PGE_2α in menstrual stage of patients (all P<0.05).Conclusion: (1) NYF has good clinical effect in treating endometriosis; (2) Patients’ symptom of menalgia is closely related with the excessive high levels of PGF_2α and PGE₂, PGI₂/TXA₂ ratio disturbance, and excessive low level of β-EP; (3) NYF could significantly decrease the PGE₂, PGF_1α levels, increase the 6-keto-PGF_1α/TXB₂ ratio and the level of β-EP, so as to alleviate the menalgia in patients with endometriosis. This item was supported by National Funds of Natural Sciences(No. 30070942)     

Clinical Study on Long-Term Treatment of Ankylosing Spondylitis with Integrative Medicine

Ａｎｋｙｌｏｓｉｎｇｓｐｏｎｄｙｌｉｔｉｓ（ＡＳ）ｉｓａｒｈｅｕｍａｔｉｃｄｉｓｅａｓｅｗｉｔｈｈｉｇｈｉｎｃｉｄｅｎｃｅ，ｉｎｄｕｃ ｉｎｇｄｉｓａｂｉｌｉｔｙａｎｄｉｎｖａｄｉｎｇｔｈｅｓｐｉｎａｌａｘｉａｌｊｏｉｎｔａｓｉｔｓｃｈｉｅｆｃｈａｒａｃｔ     

基层医院病案管理影响因素分析

本文通过对一些基层医院的了解和实地考察,对基层医院病案管理的影响因素进行了探索和分析,期望能使病案管理的质量得以提高,从而更好地为医院管理、临床医疗、科研教学和社会需求服务,达到病案管理的工作目的。     

蓝山眼科研究:老龄人群的开角型青光眼和甲状腺疾病

目的:根据甲状腺疾病的病史以及当前甲状腺素的使用情况,评估甲状腺疾病与开角型青光眼(O AG)是否存在独立的相关性。方法:蓝山眼科研究中心的患者3654例,年龄49～97岁。收集患者自述的甲状腺病史和治疗情况。眼部检查包括压平眼压计,立体视盘照像及自动视野检查。O AG的诊断根据眼压、典型青光眼的视野改变和视盘凹陷。使用多变量模型评估甲状腺疾病(病史和治疗)和OA G的相关性。结果:324例有甲状腺病史的患者(占8.9%)中,147例(占4.0%)正在使用甲状腺素。尽管难以对所有病例的甲状腺功能异常进行精确分类,但甲状腺素的使用提示患者曾有…     

肾综合征出血热血清甲状腺素变化的临床意义

本文通过20例肾综合征出血热(HFRS)37次血清T2、T4值的检测，并与20例健康献血者血清T3、T4检测值比较．发现发病早期(5病日内)各型HFRS血清T3值均低于正常组，中型、童型患者T4值与正常组比较有显著性差异(P＜0．05)．。T3、T4值，发热期出现降低，低血压休克期和少尿期下降最为明显，多尿期开始恢复，恢复期T3值接近正常，T4值反而升高，与对照组比较有非常显著性差异(P＜0．01)．提示，对HFRB患者进行血清T3、T4的检测，对HFRS的早期分型和判断预后有一定的临床意义。     

发挥中药临床基地优势加强祖国医药学的研究

为促进中药事业的发展,发扬我国传统医药学所独具的特点和优势,充分发挥中医中药专家的作用,积极应用现代先进科学技术,搞好中药的临床研究,提高中药新药的研制水平,做好中药新药审批、老药再评价以及药品不良反应的监测等工作,卫生部进行调查研究后根据中医药特点和各地的优势,结合实际     

Investigation of the Effect of Neoadjuvant Chemotherapy on Stage Ⅱ Breast Cancer

OBJECTIVE To investigate the effect of neoadjuvant chemotherapy in treatment of Stage Ⅱ breast cancer. METHODS The data from 113 patients with breast cancer of the same pathologic type in Stage Ⅱ,during the period of 1995 to 2001,were analyzed retrospectively.Among the patients,47 were treated with neoadjuvant chemotherapy,and 66 received no adjuvant therapy before surgery（control group）.After the patients of the neoadjuvant chemotherapy group had received 2 courses of chemotherapy with the CMF regimen,the surgical procedure was conducted. RESULTS Complete remission（CR）was attained in 9 of the 47 cases receiving neoadjuvant chemotherapy and partial remission（PR）was reached for 22 cases.The rate of breast-conserving surgery was enhanced from 22.73%to 46.81%（P〈0.05）in the neoadjuvant treatment group. There was no difference in the 5-year overall survival（OS）and disease-free survival（DFS）rate between the two groups（P〉0.05）,but the 5-year OS and DFS of the cases with clinical tumor remission was higher compared to the control group（P〈0.05）. CONCLUSION Neoadjuvant chemotherapy can enhance the rate of breast conservation for Stage Ⅱ breast cancer and may improve the prognosis of the cases with clinical remission.