7832例孕妇HCMV-IgM的检测分析

目的为了解孕妇人巨细胞病毒（HCMV）近期感染状况。方法采用酶联免疫吸附试验（ELISA）对7832例孕妇的静脉血标本进行了HCMV-IgM检测。结果在7832例孕妇中检测出HCMV-IgM阳性标本67例,阳性率为0.86%。结论在孕妇中巨细胞病毒有一定的近期感染率。     

磷酸组织胺诱导的Ⅰ型超敏反应对肝损伤作用的研究

目的:通过观察与Ⅰ型超敏反应相关的生物活性介质--组织胺对家兔肝脏有无直接损伤作用,进一步论证Ⅰ型超敏反应对肝脏的损伤作用.方法:选择34只家兔随机分为对照组、实验Ⅰ组和实验Ⅱ组3组;对照组只进行正常饲料喂养,实验Ⅰ组在正常饲料喂养的同时每天给予0.4μg/kg耳静脉注射磷酸组胺注射液,实验Ⅱ组在正常饲料喂养的同时每天给予0.08 μg/kg耳静脉注射磷酸组胺注射液;动态观察以上3个组的血清谷丙转氨酶(ALT)及血清谷草转氨酶(AST)变化;利用光学显微镜观察以上3个组肝组织的病理变化.结果:无论是实验Ⅰ组或实验Ⅱ组,经过一段时间的观察,发现血清内ALT和AST含量均显着高于对照组(P＜0.01),但Ⅰ、Ⅱ组之间无显着性差异(P＞0.05);实验Ⅰ组和实验Ⅱ组在显微镜下观察,其肝脏均有不同程的损伤和病理改变,且实验Ⅱ组的损伤和变化大于实验Ⅰ组,而对照组的肝脏则无明显的病理变化.结论:组织胺对家兔肝脏确实有一定的损伤作用,而且随着投予剂量和时间的增加,肝脏的损伤和病理变化也越显著;通过本研究,可以得出Ⅰ型超敏反应导致肝脏病理变化及损伤的见解.     

Interferon-gamma is an autocrine mediator for dendritic cell maturation

Maturation of dendritic cells (DC) is critical for efficient antigen presentation and initiation of an immune response. Interferon-gamma (IFN-gamma) is an important Th1 cytokine. In this study, we investigated the role of IFN-gamma in DC maturation using either IFN-gamma receptor deficient- or IFN-gamma overexpression-models. We showed that immature DC generated in vitro from bone marrow (BM) progenitor cells produced low level of IFN-gamma. After LPS stimulation, DC produced more IFN-gamma, and IFN-gamma productions were at comparable levels among C57BL/6 mice-derived DC (C57BL/6 DC), wild-type 129 mice-derived DC (129 DC) and IFN-gamma receptor deficient 129 mice-derived DC (IFN-gammaR-/-DC). We found that IFN-gammaR-/-DC exhibited decreased expression of CD54, CD86, reduced capacity to secrete IL-1beta and IL-12p70, and impaired capacity to stimulate alloreactive T cells and to drive Th1 differentiation. Transfection of IFN-gamma gene into DC promoted DC to express higher CD40, CD54, CD80, CD86, CCR7 and I-Ab, secrete more IL-1beta and IL-12p70, and more potently activate both CD4 and CD8 T cells. These data suggest that IFN-gamma signaling pathway is important for the maturation of DC in an autocrine fashion.     

抗SARS-CoV抗原的人源Fab段噬菌体抗体库的构建

目的 :利用抗SARS冠状病毒IgG抗体阳性的SARS康复患者外周血淋巴细胞 ,构建人源Fab段抗体文库。方法 :制备外周血淋巴细胞总RNA ,逆转录成cDNA。以其为模板 ,利用针对家族特异性Ig基因的引物扩增重链Fd段和轻链基因 ,并重组到噬菌粒载体pComb3中 ,将重组噬菌粒载体电转化大肠杆菌XL 1Blue,酶切鉴定抗体库的重组率 ,并测定噬菌体抗体库的库容量。结果 :构建了源于SARS康复患者血清中抗Fab段的抗体文库 ,轻链、重链Fd段基因的重组率分别为91%和 75 % ,库容量为 7.2 3× 10 7。结论 :成功地构建了抗SARS CoV抗原的人源Fab段噬菌体抗体库     

Association of Opioid Use Disorder With 2016 Presidential Voting Patterns: Cross-sectional Study in New York State at Census Tract Level

BackgroundOpioid overdose-related deaths have increased dramatically in recent years. Combating the opioid epidemic requires better understanding of the epidemiology of opioid poisoning (OP) and opioid use disorder (OUD).ObjectiveWe aimed to discover geospatial patterns in nonmedical opioid use and its correlations with demographic features related to despair and economic hardship, most notably the US presidential voting patterns in 2016 at census tract level in New York State.MethodsThis cross-sectional analysis used data from New York Statewide Planning and Research Cooperative System claims data and the presidential voting results of 2016 in New York State from the Harvard Election Data Archive. We included 63,958 patients who had at least one OUD diagnosis between 2010 and 2016 and 36,004 patients with at least one OP diagnosis between 2012 and 2016. Geospatial mappings were created to compare areas of New York in OUD rates and presidential voting patterns. A multiple regression model examines the extent that certain factors explain OUD rate variation.ResultsSeveral areas shared similar patterns of OUD rates and Republican vote: census tracts in western New York, central New York, and Suffolk County. The correlation between OUD rates and the Republican vote was .38 (P<.001). The regression model with census tract level of demographic and socioeconomic factors explains 30% of the variance in OUD rates, with disability and Republican vote as the most significant predictors.ConclusionsAt the census tract level, OUD rates were positively correlated with Republican support in the 2016 presidential election, disability, unemployment, and unmarried status. Socioeconomic and demographic despair-related features explain a large portion of the association between the Republican vote and OUD. Together, these findings underscore the importance of socioeconomic interventions in combating the opioid epidemic.     

rGH＋CLS减少电损伤组织坏死的研究

研究rGH CLS局部应用对减少高压电损伤组织进行性坏死的作用。使用该实验室已建立的重度高压电损伤模型,新西兰大白兔45只均分5组。治疗分别使用rCH CLS（1组）,CLS（2组）,rGH(3组）,盐水（4组）和5组对照,临床解剖探查、组织学和超微结构等方法观察。结果：治疗1组有明显的减少组织坏死作用。15d动物伤肢完整,而2,3和4组虽也各有不同程度延缓坏死的作用。但动物伤肢最终坏死丧失,5组7d大部软组织基本坏死,12d坏死离断,光电镜检查1组5d治疗后粒细胞浸润较少,炎性反应轻,有纤维组织细胞再生和新生的线粒体,10d修复过程明显,2,3,4组7d后坏死明显,5组肌坏死迅速,结果表明：CLS持续组织内透析冲洗和rGH局部肌注能有效地减少电损伤坏死速度和范围,值得研究应用。     

肝移植病人的ICU期观察及护理

目的：通过对8例肝移植、1例肝肾联合移植术后护理经验的总结,介绍肝移植病人的护理重点及方法;方法：隔离室准备、神经系统及生命体征监测、排斥反应监测、预防感染及心理护理;结果：肝移植术后早期（ICU）的护理至关重要,直接关系到近远期疗效和生存率;结论：新技术的开展要求高素质的护理人员和高质量的护理水平。     

雷尼替丁与复方丹参联合治疗急性胰腺炎35例疗效分析

目的：观察雷尼替丁与复方丹参联合治疗急性胰腺炎的疗效。方法：对确诊急性胰腺炎65例随机分成治疗组35例,对照组30例,分别用常规治疗,常规治疗加雷尼替丁与复方丹参静滴,连用4－7天,评价其疗效。结果：治疗组与对照组显效率分别为85.71%、43.3%,两组差异显著,P＜0.05。结论：两药联合应用不仅可减轻本病的炎症病变,且可改善心肌细胞营养状况。     

饮食服务业从业人员乙型肝炎病毒感染动态研究

目的为了解泰安市饮食服务业从业人员乙型肝炎病毒感染状况。方法应用ELISA方法在1995～1999年对53434名饮食服务业从业人员进行了血清HBsAg、抗-HBc、HBeAg和抗-HBe检测。结果1995～1999年饮食服务业从业人员血清HBsAg、抗-HBc、HBeAg和抗-HBe总阳性率呈逐年下降趋势,依次为4.41%、3.88%、3.46%、2.86%和2.50%,其差异有显著意义（χ     

0．75％罗哌卡因硬膜外麻醉用于妇产科手术的临床观察

目的 观察０．７５％罗哌卡因硬膜外用于妇产科手术的临床效果、方法 选择７２例ＡＳＡＩ－Ⅱ级妇产科手术随机分为两组．Ｉ组为０．７５％罗哌卡因组（３６例）Ⅱ组为０．５％布比卡因组（３６例）,两组均取腰１－２行硬膜外穿刺,观察两组的感觉阻滞、运动阻滞、镇痛效果及对循环呼吸的影响和不良反应,结果 两组感觉阻滞达Ｔ８的时间。切皮时ＶＡＳ评分、运动恢复时间有显著性差异（Ｐ＜０．０５）,不良反应中寒颤的发生率Ⅰ组明显比Ⅱ组少（Ｐ＜０．０５）,结论０．７５％罗哌卡因具有起效时间快,镇痛效果好,运动阻滞恢复早,对循环呼吸的影响小,神经毒性低的临床特点。