脑卒中女性患者的盆底结构及盆底肌电特征

目的 探讨脑卒中对女性患者盆底结构和功能的影响。方法 2020年6月至12月,东南大学附属中大医院住院的脑卒中女性患者21例(卒中组)分为尿失禁组(n=6)和非尿失禁组(n=15),另选取同龄健康女性志愿者20例作为对照组。所有受试者行经会阴盆底超声和盆底表面肌电检查。结果 尿失禁组和非尿失禁组平均肌电值、积分肌电值、均方根值、平均功率频率和中位频率均低于对照组(P <0.05),尿失禁组与非尿失禁组间无显著性差异(P> 0.05)。三组间膀胱颈位置,膀胱尿道后角,膀胱颈移动度,尿道旋转角,以及Valsalva动作后肛提肌裂孔前后径、左右径和面积均无显著性差异(F <2.484, P> 0.05)。结论 无论是否存在尿失禁,脑卒中女性患者盆底肌活动均有不同程度下降,盆底支持组织的结构尚未发生明显改变。     

牙本质仿生矿化的研究进展

牙本质仿生矿化是口腔医学和生物材料学领域关注的热点.通过仿生矿化途径得到在组成、结构和功能等方面与天然牙本质高度一致的仿生材料,从而实现牙本质的仿生修复,具有重要的临床意义和和广阔的应用前景.本文就近年来牙本质仿生矿化的研究进展作一综述.     

Short-term effect of high volume hemofiltration on treatment of critically ill associated with acute kidney injury

目的 探讨高容量血液滤过(HVHF)用于危重症合并急性肾损伤(AKI)治疗的近期疗效.方法 120例危重症合并AKI患者行HVHF治疗,监测治疗前后患者生命征及血电解质、血糖、肾功能变化,同时分别在治疗前后对患者行简化急性生理评分(SAPSⅡ)、急性肾小管坏死-个体严重程度指数(ATN-ISI)评分,并进行统计学分析.结果 治疗结束后患者一般情况稳定,平均动脉压(MAP)显著上升,肾功能指标显著好转,ATN-ISI及SAPSⅡ评分显著下降.结论 HVHF用于危重症合并AKI患者的治疗有良好的近期疗效.     

四种牙本质粘结剂粘结强度的比较研究

目的 比较四种牙本质粘结剂(I-Bond、G-Bond、Xeno V和Clearfil S3 Bond)的粘接强度,为临床上正确选择、合理使用粘接剂提供实验依据.方法 60颗离体前磨牙,随机分为4组,每组15颗.将四种粘结剂分别用于暴露的牙本质表面,修复树脂“冠”,光照固化.用CMT7104型万能材料测试仪测试试件微拉伸和剪切粘结强度,比较四种牙本质粘结剂的粘结强度.结果 四种粘结剂的粘结强度均值依次为Xeno V＞Clearfil S3 Bond＞G-Bond＞I-Bond.其中,I-Bond与G-Bond的粘结强度差异无显著性(P＞0.05);Xeno V和Clearfil S3 Bond的粘结强度差异亦无显著性(P＞0.05);而I-Bond和G-Bond的粘结强度明显低于XenoV和Clearfil S3 Bond (P＜0.05).结论 四种牙本质粘结剂中,Xeno V和Clearfil S3 Bond的粘结强度明显高于I-Bond和G-Bond的粘结强度.     

三磷酸腺苷抑制肿瘤坏死因子-α诱导的肌源性干细胞凋亡及机制

目的 探讨三磷酸腺苷(ATP)对肌源性干细胞(MDSCs)凋亡的抑制作用及其机制.方法 采用差速贴壁法分离新生小鼠MDSCs,利用40μg/L肿瘤坏死因子(TNF)-α诱导MDSCs凋亡,100 μmol/L ATP抑制细胞凋亡.经流式细胞仪检测MDSCs的凋亡率和半胱氨酰天冬氨酸特异性蛋白酶( Caspase) -3/8的表达.结果 TNF-α诱导后,脱壁细胞明显增多,MDSCs的凋亡率由5.17％升至31.58％,增加了5倍,Caspase-3染色阳性细胞率由5.01％增至19.25％,Caspase-8染色阳性细胞率由2.97％增至8.58％.ATP作用后,MDSCs的凋亡率降至12.06％,减少了2/3,Caspase-3染色阳性细胞率降至9.86％,Caspase-8染色阳性细胞率降至6.96％,但仍高于对照组.结论 ATP能抑制MDSCs表达Caspase-3/8,减轻TNF-α诱导的MDSCs凋亡.     

Late Effects in Mouse Heart after 60Co γ-irradiation of the Brain: An Ultrastructural Study

Summary

A single dose of 8 or 20 Gy ⁶⁰Co γ-rays was given to C3H male mice at 4 months of age. Degenerative changes in the cardiac muscle due to brain irradiation were observed first at 6 months after irradiation, and became progressively more severe at 12–24 months. The changes seen at the ultrastructural level included myofibrillolysis, the presence of lysosomal-like bodies and interstitial fibrosis. Ultrastructural changes in the control cardiac muscle throughout the experimental period were monitored and only minor aging changes were noted. The coronary arteries of control mice began to show a slight amount of smooth muscle degeneration and fibrosis 1 year into the experiment. At 18 months the lesions became more severe, and at 24 months there was relatively less distinction between the control and the 20 Gy treated group. Degenerative changes in the coronary arteries were noticed at 6 months after irradiation, and became progressively more severe at later times (12–24 months). The major changes included smooth muscle degeneration with fibrosis and the accumulation of debris and extracellular matrix. At 18 months the medial smooth muscle showed severe damage, with accumulations of matrix material and debris. There was additional fibrosis in the adventitial layer. There were few additional changes at 24 months after 20 Gy irradiation. Quantitative analyses indicated that the average fractional volumes of degenerated smooth muscle cells were 13, 27 and 39% in the unirradiated group at 12, 18 and 24 months, respectively, and 13 and 29% in the sham-irradiated group at 12 and 18 months into the experiment, respectively. These percentages were 12, 32 and 49% (P < 0·05) after 8 Gy irradiation, and 19% (P < 0·05), 46% (P < 0·01), and 42% after 20 Gy irradiation, respectively.     

2010、2011年广州市越秀区空气污染物AQI与呼吸系统疾病死亡的Poisson回归分析研究

[目的]探讨广州市越秀区空气污染物AQI对呼吸系统疾病死亡的影响。[方法]收集2010、2011年广州市越秀区呼吸系统疾病死亡数据和越秀区气象及大气污染物数据,采用广义线性模型模块,拟合Poisson回归分析空气各污染物空气质量指数（AQI）、综合AQI和空气质量分级与呼吸系统疾病死亡的影响。[结果]2010、2011年广州市越秀区因呼吸系统疾病死亡病例有2 678例。SO2AQI与呼吸系统疾病死亡有统计学意义,SO2AQI每升高1个单位,其危险度约增加1%（RR=1.01,P〈0.05）;综合AQI的变化和空气质量等级与呼吸系统疾病死亡无统计学意义。[结论]广州市越秀区空气污染物中SO2是呼吸系统相关因素死亡的危险因素。     

DBC1的研究进展

DBC1是近年来新发现的一种蛋白,属于小GTP酶类Rho家族的非典型成员。最初因为在乳腺癌组织中杂合性缺失被鉴定出来的。目前关于DBC1在乳腺癌上的研究报道尚少。本文对国内外关于该基因的有关研究进行综述,了解其分子机制,基因表达情况。