SVEP1 plays a crucial role in epidermal differentiation

SVEP1 is a recently identified multidomain cell adhesion protein, homologous to the mouse polydom protein, which has been shown to mediate cell‐cell adhesion in an integrin‐dependent manner in osteogenic cells. In this study, we characterized SVEP1 function in the epidermis. SVEP1 was found by qRT‐PCR to be ubiquitously expressed in human tissues, including the skin. Confocal microscopy revealed that SVEP1 is normally mostly expressed in the cytoplasm of basal and suprabasal epidermal cells. Downregulation of SVEP1 expression in primary keratinocytes resulted in decreased expression of major epidermal differentiation markers. Similarly, SVEP1 downregulation was associated with disturbed differentiation and marked epidermal acanthosis in three‐dimensional skin equivalents. In contrast, the dispase assay failed to demonstrate significant differences in adhesion between keratinocytes expressing normal vs low levels of SVEP1. Homozygous Svep1 knockout mice were embryonic lethal. Thus, to assess the importance of SVEP1 for normal skin homoeostasis in vivo, we downregulated SVEP1 in zebrafish embryos with a Svep1‐specific splice morpholino. Scanning electron microscopy revealed a rugged epidermis with perturbed microridge formation in the centre of the keratinocytes of morphant larvae. Transmission electron microscopy analysis demonstrated abnormal epidermal cell‐cell adhesion with disadhesion between cells in Svep1‐deficient morphant larvae compared to controls. In summary, our results indicate that SVEP1 plays a critical role during epidermal differentiation.     

实现医院X光片共享信息系统的安全策略

随着医院信息化建设的深入发展,为保证医院数据的连续性、完整性和准确性,信息系统需要24h不间断运行。因此,如何保证信息系统正常运行及信息安全,就成为一个重要问题,涉及到网络、环境、人等各方面因素。     

一体化创伤急救护理模式的探索

探索急救护理模式新理念，通过对我院急救部护理工作模式即院前急救一院内急救一救命性手术配合一创伤病房服务一重症监护整体救护机制构建、急救专科护士定位与培养、急救护理临床快速反应流程建立、急救护理质量规范化控制与管理、创伤后心理应激救援与健康教育的运行实践，阐述了急救护理模式从传统型向现代型转变，构建一体化急救护理模式可使得急诊科护士从简单通用型护理，向着急救护理专科型专业化转变的迫切性、必要性和可行性。     

microRNA的液体芯片检测方法

microRNA表达谱的检测需要精确、高通量和多重检测方法 ,液体芯片可通过编码微球结合探针的序列差异,来检测相应的microRNA序列。本文介绍了两代液体芯片背景的FlexmiR产品,其通过锁核酸,逐步降温和单链酶解来提升对microRNA检测的特异性,为临床科研microRNA的研究提供新的检测工具。     

Dynamic combinatorial synthesis of a catenane based on donor–acceptor interactions in water

A new type of neutral donor–acceptor [2]-catenane, containing both complementary units in the same ring was synthesized from a dynamic combinatorial library in water. The yield of the water soluble [2]-catenane is enhanced by increasing either building-block concentrations or ionic strength, or by the addition of an electron-rich template. NMR spectroscopy demonstrates that the template is intercalated between the 2 electron-deficient naphthalenediimide units of the catenane.     

中药游泳加抚触对新生儿黄疸的影响

目的探讨中药游泳加抚触对新生儿黄疸的影响。方法通过对2009年1～3月新生儿随机分为中药游泳加抚触组60例和单纯清水游泳组60例,观察新生儿出生6 d内黄疸状况和抚触后婴儿皮肤完整性和脐部情况。结果观察组新生儿胎便初排和转黄时间明显提前,黄疸程度轻于对照组,观察组无1例出现不良反应。结论中药游泳加抚触有助于新生儿生理性黄疸的消退和减少新生儿病理性黄疸的发生,方便有效,无不良反应。