微分脉冲伏安法测定奥美拉唑片中主药含量

目的:建立以微分脉冲伏安(DPV)法测定奥美拉唑片中奥美拉唑含量的方法。方法:分析了奥美拉唑不同电化学分析方法下的不同伏安行为,确定最佳分析方法及条件。结果:最佳分析方法为DPV法,在0.1mol/L醋酸-醋酸钠(HAc-NaAc)缓冲液中奥美拉唑检测浓度在1.0～40.0mg/L范围内与该峰电流值呈良好的线性关系(r=0.9904),其最低检测限为0.19mg/L,回收率为99.3%～102.0%。结论:DPV法测定奥美拉唑含量灵敏度较高,检测限较低,可望成为标准测试方法。     

Endoscopic transnasal canaliculorhinostomy for refractory common canalicular obstruction with an unidentifiable lacrimal sac

AIM: To compare the safety and efficacy of conventional versus accelerated (9 mW/cm2) CXL in progressive keratoconus at the 2-year follow-up. METHODS: In this prospective study, consecutive progressive keratoconus patients were randomized to receive either conventional CXL (CCXL) or accelerated CXL (ACXL, using hydroxypropyl methylcellulose-assisted riboflavin imbibition for 10min) at 9 mW/cm2. Visual, refractive, keratometric, topographic, and aberrometric outcomes and stromal demarcation line depth (DLD) measurements were compared at the end of a 2-year follow-up. RESULTS: Thirty-two eyes from 32 patients in the CCXL and 27 eyes from 27 patients in the ACXL groups completed 2-year follow-ups. At 2 years post-CXL, both uncorrected and corrected visual acuities improved significantly in both groups. The improvements in keratometric readings, flattening rate (flattening of the Kmax more than 1D), 3 topographic indices, and vertical coma were significantly better in the CCXL group compared to the ACXL group (P<0.05). The DLD as measured by anterior segment optical coherence tomography or in vivo confocal microscopy was better detectable and significantly deeper in the CCXL group compared to the ACXL group. The deeper DLD was found to be significantly correlated with improvements in the mean keratometry measurements. Progression was noted in 11.1% of eyes in the ACXL group, whereas progression was not observed in any patient eye in the CCXL group. CONCLUSION: In this prospective randomized study, ACXL was less effective in halting the progression of keratoconus at a 2-year follow-up compared to CCXL.     

灯盏花素对大鼠脑缺血再灌注后血管活性物质的影响

目的观察灯盏花素对大鼠急性脑缺血再灌注后血浆和脑组织ET、TXB2、6-keto-PGF1α含量的影响。方法选用Wistar大鼠制作Pulsinelli四血管阻断(4VO)脑缺血再灌注模型,以川芎嗪为对照,采用放射免疫法测定灯盏花素对大鼠脑缺血再灌注后血浆和脑组织ET、TXB2、6-Keto-PGF1α含量的影响。结果灯盏花素能显著降低脑缺血再灌注后血浆和脑组织ET、TXB2含量及TXB2/6-Keto-PGF1α比值。结论灯盏花素通过改变血管活性物质的含量从而对急性脑缺血再灌注损伤具有保护作用。     

武当山何首乌及其混伪品的鉴定研究进展

通过对武当山何首乌作用情况的调查,发现何首乌混伪品５种;将头花千金藤误作何首乌钐,极易发生中毒,就彻底纠正;将凝胶电泳、ＵＶ、ＩＲ光谱技术应用于何首乌及其混伪品鉴别之中,编制出鉴别检索表,使鉴定方法更趋完善;“人形雌雄何首乌”属无稽之谈,植物块根不可能分化出人体五官、性别特征;经考证,古时的何首乌混淆品宕芋,即为现今的冀蓼;探讨何首乌种混淆的历史渊源,分析将“雌雄”引用于中药名称中的利与弊,防止产     

Xanthone glycosides from Polygala tenuifolia and their conformational analyses

Seven xanthone glycosides were isolated from the cortexes of Polygala tenuifolia, and their structures were identified as polygalaxanthones VIII-XI (1-4), sibiricoxanthone B (5), 7-O-methylmangiferin (6), and lancerin (7), on the basis of spectroscopic analyses. Compounds 1-4 are new xanthone glycosides, and compounds 4 and 5 exist as rotamers. To explain this phenomenon, conformational analyses were performed on compounds 4 and 5 and other compounds with similar skeletons that were isolated from P. tenuifolia.     

中风康胶囊治疗急性脑梗塞临床与实验研究

目的：观察中风康胶囊治疗急性脑梗塞的临床疗效及对线栓法造成的大鼠局灶性脑缺血后再灌注损伤的治疗作用。方法：采用双盲法随机对照观察,设中风康胶囊治疗组30例和尼莫地平对照组30例,动物实验时,建立线栓法引起大鼠局灶性脑缺血模型。在缺血后4h进行再灌注,可引起大鼠产生明显的行为障碍和局灶性脑梗塞,用中风康胶囊口服,并同尼莫地平片进行比较。结果：治疗组总有效率93．33％,对照组总有效率66．67％（P＜0．05）,中风康组疗效优于尼莫地平组;治疗组治疗后血液流变学,血脂较治疗前和对照组治疗后明显下降（P＜0．05）,实验研究证实,中风康胶囊对大鼠线栓法造成的局灶性脑缺血损伤具有明显的治疗作用。结论：中风康胶囊治疗急性脑梗塞有较好的疗效。     

瑞氏-姬姆萨染色不同时间和不同浓度对正常成人红细胞形态测量结果的影响

目的探讨不同染色时间和试剂浓度对细胞形态测量结果的影响情况。方法以瑞氏一姬姆萨染色的红细胞为研究对象,采用不同染色时间（10、20、40min）和A、B液比例（1：1、1：2、1：4）对血涂片进行染色,应用图像分析软件分别测量红细胞的红、绿、蓝三基色和面积、平均直径、周长,并对测量结果进行分析比较。结果不同染色时间和试剂浓度组红细胞红、绿、蓝三基色差异均有统计学意义（P〈0．05）,其中红、绿、蓝基色值在短时间（10min）和低浓度（1：4）组最小,在长时间（40min）和高浓度（1：1）组最大（P〈0．05）。不同染色时间和试剂浓度组红细胞的面积、平均直径、周长差异均没有统计学意义（P〉0．05）。结论染色时间和试剂浓度对细胞色度学参数影响明显,而对几何形态学参数无明显影响。     

外周血 KISS1 mRNA对上皮性卵巢癌的诊断价值

目的:通过与CA125比较,探讨外周血KISS1 mRNA在上皮性卵巢癌(epithelial ovarian cancer,EOC)中的诊断价值。方法:采集2010年1月至2014年1月在南通大学第二附属医院住院拟手术的EOC患者40例手术前一天的外周静脉血和正常健康者静脉血,用RT-PCR法和电化学发光法分别检测外周血KISS1 mRNA和CA125的表达。结果:EOC早期组(Ⅰ~Ⅱ期)、晚期组(Ⅲ~Ⅳ期)KISS1 mRNA的表达量均显著高于正常健康者(均P<0.01),而早期与晚期EOC组间比较无显著差异(P>0.05);EOC晚期组CA125的表达量明显高于早期组及正常健康者(均P<0.01),而早期组与正常健康者比较无显著差异(P>0.05)。KISS1 mRNA ROC(receptive operator character curve,ROC)曲线的切值(cutoff)设为0.51、0.72时,阳性预测值(positive predictive value,PPV)分别为0.58和1,阴性预测值(negative predictive value,NPV)分别为0.92和0.7;CA125的cutoff值设为20、100 U/ml时,PPV分别为0.72和1,NPV分别为0.87和0.81。KISS1 mRNA、CA125对EOC均具有中度诊断效能(P=0.34);两者比较,KISS1 mRNA对早期EOC具有较高诊断价值(P=0.018),而CA125对晚期EOC的诊断价值较高(P<0.01)。结论:患者外周血KISS1 mRNA与CA125对EOC具有同等的诊断价值,KISS1 mRNA可作为EOC的一个新的肿瘤标志物;两者的联合检测可提高EOC的诊断效能,特别是早期EOC的诊断率。     

扶桑花抗生育成分对早孕小鼠黄体影响的定量研究

本文处用图像分析仪 ,以核浆比和数密度为指标 ,研究了不同浓度的扶桑花提取物—HR 1对早孕小鼠黄本组织学的影响。结果表明 :(1)小鼠黄体细胞的核浆比和数密度 ,随给药剂量 (0、 4、 10、 10 0、10 0 0mg/kg/d)的增加而增加 ,其中 10 0和 10 0 0mg/kg/d两组的黄体细胞核浆比和数密度明显高于对照组(p <0 .0 5) ;(2 )黄体组织学的定性观察显示 ,给药各组的黄体细胞明显退化 ,细胞缩小 ,细胞界限不清。这些结果提示 ,扶桑花提取物的抗早孕作用与妊娠黄体受损有关。     

胞苷脱氨酶基因对小鼠大剂量化疗的保护作用

Objective:To explore the feasibility of transfecting cytidine deaminase(CD)gene into mouse bone marrow cells in order to observe the drug resistance of high dose Ara-C and improve the tolerance of myelosuppression following combination chemotherapy.Methods:Human cytidine deaminase gene was transfected into mice bone marrow cells by retroviral vector.Resistant colony-forming unit granulocyte-macrophage(CFU-GM)assay was performed after the transfected mice bone marrow cells treated by the Ara-C.DNA was extracted from mice bone marrow cells.The drug resistant gene in mice bone marrow cells after transfection was detected by PCR.Results:Bone marrow cells of lhe donor mice cultured with lhe retroviral producer cells showed the drug resistant colonies and resistance to Ara-C,so did accept mice transplanted with the CD gene(CFU-GM of donor mice was 52%,X2=124.62,P＜0.01:accept mice was 54%,X2=126.26.P＜0.01,both compared with the contrast group).The animal survival rate was significantly higher in gene transfected group than that of the control(X2=7.42.P＜0.01).CD gene of transfected bone marrow cells was confirmed by PCR.Conclusion:CD gene can be transfected into bone marrow cells of mice efficiently and increase the drug resistance to Ara-C.