浅析肉类罐头杀菌工序的确认和验证

〔目的〕保证肉类罐头食品安全卫生。通过对杀菌工序的确认和验证达到保证罐头食品商业无菌的目的。〔方法〕分析杀菌工序确认与验证的关系,探讨杀菌工序确认与检验的具体方法。〔结果〕对杀菌设备的备案检查及杀菌操作的确认是杀菌工序投入生产的关键;只有经过文件验证和现场检查审核验证才能确保罐头食品安全卫生。〔结论〕杀菌工序的确认和验证两者之间相辅相成,缺一不可,它是对HACCP管理体系的验证,是保证产品和生产过程中消除各种危害因素的关键,是提高产品质量,增加出口经济效益的有力保证。     

Nerve growth factor and tyrosine kinase A in human salivary adenoid cystic carcinoma: expression patterns and effects on in vitro invasive behavior.

PURPOSE: Perineural invasion is a frequent occurrence in salivary adenoid cystic carcinoma (ACC) and may prevent complete surgical resection. Studies have indicated that nerve growth factor (NGF) and its high-affinity receptor tyrosine kinase A (TrkA) may play a role in perineural invasion in several malignancies in which perineural invasion is observed. The present study was conducted to investigate the expression of NGF and TrkA in salivary ACC and to examine the effects of NGF on adhesion, migration and invasion capacities of a salivary ACC cell line (SACC-83) in vitro. PATIENTS AND METHODS: Expression of NGF and TrkA was explored using immunohistochemistry in paraffin-embedded tissues of 32 cases of salivary ACC. The effects of NGF on in vitro adhesion, migration, and invasion capacities of the SACC-83 cell line were examined using an MTT assay and a modified Boyden chamber assay respectively. RESULTS: In ACC specimens, 31 (96.9%) and 32 (100%) tumors showed immunoreactivity for NGF and TrkA respectively. Significant correlations were found between NGF/TrkA expression levels and perineural invasion (P < .05). In cell adhesion assay, the percent adherences of SACC-83 cells co-cultured with 25 ng/ml NGF at 1.5 hours and 5, 25 ng/ml NGF at 6 hours were significantly higher than that co-cultured with 0 ng/ml NGF (P < .05). However, high concentration of NGF (500 ng/ml) resulted in a significant inhibition of invasion (P < .05). CONCLUSION: Overexpression of NGF and TrkA in human salivary ACC tissues may constitute a reason for perineural invasion in salivary ACC.     

γ射线照射对血小板活化的影响

目的:探讨γ射线照射对单采血小板保存过程中血小板活化的影响.方法:用细胞分离机单采血小板20人份,以27Gyy射线照射,标准条件下保存5天,间隔取样分别用RIA,ELISA方法检测血小板表面P-选择素(P-Selectin)、血浆中可溶性P-选择素(sP-Selectin)及β-血小板球蛋白(β-TG)含量.结果:在保存3d,5d后,照射组及对照组中血小板表面P-Selectin含量、血浆中sP-Selectin及β-TG含量与保存前比较差异显著(P＜0.05),但是两组间比较无统计学差异(P＞0.05),结论:γ射线照射不会促进血小板的活化.     

用视诱发电位和富里叶变换作视力客观估计

目的:研究用图形视觉诱发电位(PVEP)和离散富里叶变换(DFT)作视力客观估计的方法。方法:检测对象为33只正常眼。用翻转频率为10次/秒,条纹张角分别为30、20、18、12、10、6、4和2弧分的垂直方波光栅图形刺激,记录相应的稳态图形视诱发电位。用离散富里叶交换提取WEP频谱中10Hz成分的幅值。以光栅条纹张角为横坐标、相应的VEP频谱幅值为纵坐标作关系曲线,取阈值附近的近似线性部分的数据点作直线回归,用外推法求得阈值和视力。结果:比较VEP所测之视力与用国际标准视力表所测之视力,可见在本研究中70％的受检眼VEP视力估计较准确。结论:用本研究中的VEP技术客观地测量视力可靠性较高。眼科学报1997;13:59～61。     

胸部类癌

本文报道了３１例胸部类癌，其中胸腺类癌１４例，支气管类癌１７例，伴有异位ＡＣＴＨ综合征者１７例。１２例患者发生转移（３９％），２４例行原发肿瘤全部或部分切除，术后放疗或化疗或两者均有者１７例，４例仅行探查并取活检，２８例经手术及病理证实。     

小儿肾积水Anderson－Hynes手术后并发症及其治疗

对５２例（５４侧）先天性肾盂输尿管连接部梗阻性肾积水患者行Ａｎｄｅｒｓｏｎ－Ｈｙｎｅｓ手术，对其手术的并发症进行分析，主要并发症为吻合口梗阻（３９％）和泌尿系感染（２８％）．经腹进行手术，肠梗阻发生率为１３％（３／２３）．患儿手术时年龄与手术后并发症发生率无显著性意义．     

补碘对7～12岁轻度碘缺乏病儿童脑功能及生长、发育的影响

通过半年对３５名７～１２岁轻度碘缺乏病儿童进行补碘，并与同龄、同地的正常儿童和缺碘未补碘儿童进行比较，以观察补碘对儿童脑功能及生长发育的影响。结果发现：①补碘可使儿童碘营养状况恢复正常，尿碘值由（８３．２±３．５）μｇ／Ｌ上升到（１６２．２±１．６）μｇ／Ｌ，血清Ｔ４、ＦＴ４Ｉ和ｒＴ３：也与正常儿童一致而与未补碘儿童有显著性差异（Ｐ＜０．０５）．②轻度碘缺乏病儿童智商低于正常儿童（Ｐ＜０．０１），补碘在一定程度上改善其智商水平。③轻度碘缺乏病及补碘未能明显影响儿童的生长发育，可能与儿童总营养水平较差有关。     

Ultrasound Biomicroscopic Dark Room Provocative Test

Purpose : To compare the clinical application value of Ultrasound biomicrsocpic dark room provocative test with the traditional dark room test in screening primary angle closure glaucoma (PACG).Methods: 22 eyes with PACG in prodromal stage and 30 eyes with deep anterior chamber and wide angle of 15 normal persons were observed in this trail. All 52 eyes were performed traditional dark room provocative test and ultrasound biomicorosopic darkroom test respectively. With different positive diagnostic criteria, the sensitivity of the two methods were compared using chi-squared analysis.Results : After staying in the dark room for 2 hours, In case group, IOP in 10 of 22 eyes rose more than 1. 07kPa(1kPa = 7.5mmHg), in 12 eyes less than 1.07kPa or had no changes; appositional angle closure were found by Goldmann gonioscopy in 8 eyes : 3 eyes in two quadrants, 5 eyes in more than two quadrants; the appositional angle closure was found by UBM in 15 eyes; 3 eyes in one quadrants, 5 eyes in two quadrants, 7 eyes     

螺旋神经节神经元原代培养及其免疫细胞化学鉴定

目的 建立成体哺乳动物耳蜗螺旋神经节神经元（ＳＧＮ）体外培养的细胞模型。方法 对出生后７ｄ的Ｗｉｓｔａｒ大鼠的螺旋神经节细胞进行解离与分散培养。用荧光显微镜与倒置相差显微镜观察原代培养ＳＧＮ细胞的活力以及生长与分化过程。应用链霉卵白素过氧化物酶（ＳＰ）方法和抗神经丝蛋白单克隆抗体（ＮＦＰ－ＭｃＡｂ）对ＳＧＮ进行免疫细胞化学染色鉴定。结果 幼龄Ｗｉｓｔａｒ大鼠的ＳＧＮ在体外条件下，可良好存活并有正常     

穿透性角膜移植联合瞳孔成形术的临床观察

目的观察穿透性角膜移植联合瞳孔成形术的效果。方法４８只眼行穿透性角膜移植术的同时对虹膜缺损进行修复，成形瞳孔。术后随访６～８９个月。结果植片透明者３８只眼（７９．２％），视力恢复０．１以上者３１只眼（６４．６％），瞳孔修复接近正常者４３只眼（８９．６％）。结论穿透性角膜移植联合瞳孔成形术，可减少术后并发症、改善植片的预后及视功能。对波及瞳孔的虹膜缺损，联合行瞳孔成形术效果较好。