Morphology,Microstructure, and Mechanical Properties of S32101 Duplex Stainless-Steel Joints in K-TIG Welding

In this paper, the {"type":"entrez-protein","attrs":{"text":"S32101","term_id":"422005","term_text":"pir||S32101"}}S32101 duplex stainless steel welded joints were produced by a K-TIG welding system. The weld geometry parameters under different welding speeds were analyzed by combining the morphological characteristics of the keyhole. The microstructure and impact toughness of the base metal and weld metal zone under different welding speeds were studied. The experiment results show that the welding speed has quite an effect on the geometry profile of the weld. In addition, the characteristic parameters of the keyhole can effectively predict the geometry profile of the weld. The test results prove that the microstructure, Σ3 coincidence site lattice grain boundary, and phase boundary of ferrite and austenite have an effect on the impact property of the weld metal zone. When the proportion of the austenite, Σ3 coincidence site lattice grain boundary and random phase boundary increased, the impact property of the weld metal zone also increased.     

大豆黄酮对卵巢切除大鼠鼻黏膜上皮细胞凋亡的保护作用

目的：观察双侧卵巢切除诱导大鼠鼻黏膜上皮细胞凋亡，研究尼尔雌醇、大豆黄酮对此的保护作用。方法：将60只大鼠随机分为4组：健康对照组，卵巢切除组，卵巢切除＋尼尔雌醇组，巢巢切除＋大豆黄酮组。各组动物分期分3批处死，流式细胞仪检测各组动物鼻中隔黏膜的早期凋亡细胞。结果：双侧卵巢切除后，大鼠鼻黏膜的早期凋亡细胞百分数增高，术后60d达到新的平衡。大豆黄酮、尼尔雌醇干预组与健康组比较，早期凋亡细胞无明显改变。结论：雌激素替代和大豆黄酮可通过减少凋亡细胞而保护鼻黏膜免受雌激素缺乏的损害。     

面神经水平段梳理术治疗半面痉挛

半面痉挛（HFS）病因机理不清，有神经冲动短路、局部癫痛、微血管压迫学说，尚无特效药物治疗。本文报告经鼓室探查术进路行面神经水平段减压术治疗HFS6例，平均随访1.2年，显效4例，复发2例，但程度减轻。5例术中未分离砧镫关节，听力不变。HFS行水平段神经梳理术不复杂，安全有效，是多种手术疗法中可取的一种。作者介绍提高手术效果的几点经验：①鼓室探查术外耳道鼓膜皮瓣比常现大一点，有利于暴露面神经水平段；②镫骨上层结构及砧镫关节是定位面神经骨管的重要标志；③为保持听力，一般不要分离砧镫关节；④面神经水平段骨管近卵圆窗缘较薄，可用细钩针挑开；⑤根据面痉挛病程和痉挛程度的不同，进行不同强度的梳理；⑥对病情顽固者，可行第二次梳理术。     

Light Emitting Diodes Irradiation Regulates miRNA-877-3p to Promote Cardiomyocyte Proliferation

Mammalian cardiomyocytes (CMs) maintain a low capacity for self-renewal in adulthood, therefore the induction of CMs cycle re-entry is an important approach to promote myocardial repair after injury. Recently, photobiomodulation (PBM) has been used to manipulate physiological activities of various tissues and organs by non-invasive means. Here, we demonstrate that conditioned PBM using light-emitting diodes with a wavelength of 630 nm (LED-Red) was capable of promoting the proliferation of neonatal CMs. Further studies showed that low-power LED-Red affected the expression of miR-877-3p and promoted the proliferation of CMs. In contrast, silencing of miR-877-3p partially abolished the pro-proliferative actions of LED-Red irradiation on CMs. Mechanistically, GADD45g was identified as a downstream target gene of miR-877-3p. Conditioned LED-Red irradiation also inhibited the expression of GADD45g in neonatal CMs. Moreover, GADD45g siRNA reversed the positive effect of LED-Red on the proliferation of neonatal CMs. Taken together, conditioned LED-Red irradiation increased miR-877-3p expression and promoted the proliferation of neonatal CMs by targeting GADD45g. This finding provides a new insight into the role of LED-Red irradiation in neonatal CMs biology and suggests its potential application in myocardial injury repair.     

Emerging trends in sacubitril/valsartan research: A bibliometric analysis of the years 1995–2021

Background:Sacubitril/valsartan has been approved for the treatment of heart failure (HF) patients with reduced ejection fraction; since then, it gradually became a new star drug in the therapy of HF. Nevertheless, the effectiveness of sacubitril/valsartan remains under investigation. Thus far, only a few bibliometric studies have systematically analyzed the application of sacubitril/valsartan.Methods:Publications on sacubitril/valsartan were retrieved from the Web of Science Core Collection on April 29, 2021. Data were analyzed using Microsoft Excel 2019 (Redmond, WA), VOS viewer (Redmond, WA), and Cite Space V (Drexel University, Philadelphia, PA).Results:A total of 1309 publications on sacubitril/valsartan published from 1995 to 2021 were retrieved. The number of publications regarding sacubitril/valsartan increased sharply in the last 6 years (2015–2021), and American scholars authored >40% of those publications. Most were published in the European Journal of Heart Failure, the United States was the bellwether with a solid academic reputation in this area. Solomon published the highest number of related articles and was the most frequently cited author. “Heart failure” was the leading research hotspot. The keywords, “inflammation,” “fibrosis,” and “oxidative stress” appeared most recently as research fronts.Conclusions:Research attention should be focused on clinical trial outcomes. Considering its effectiveness in HF, the mechanisms and further applications of sacubitril/valsartan may become research hotspots in the future and should be closely examined.     

Effects of electroacupuncture on hippocampal and cortical apoptosis in a mouse model of cerebral ischemia-reperfusion injury

Objective: To observe the effects of electroacupuncture on hippocampal and cortical apoptosis in a mouse model of cerebral ischemia-reperfusion injury.Methods: Mouse models established by repeated cerebral ischemia-reperfusion,followed by electroacupuncture at Shenshu,Geshu,and Baihui points.The control group mice were intragastrically administered Hydergine.On day 1 and 7 post-treatment,hippocampal and cortical apoptosis was detected by terminal-deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL),and apoptosis images in the hippocampal CA1 zone and cortical area were analyzed.Results: In the model group,apoptotic cells were detected one day after treatment and some cellular fibers were disarrayed.By day 7 post-treatment,there was an increase in the number of apoptotic cells in the hippocampal CA1 region.In addition,there were apoptotic cells in the cortical area,the cortical Jayers were thinner with localized neuronal loss and sieve-like lymphocyte infiltration,as well as glial cell proliferation and visible infarct lesions.However,in the Hydergine and electroacupuncture groups,there was a small number of apoptotic cells.At 7 days post-treatment in the model group,field number,numerical density on area,and surface density were increased.However,in the Hydergine and electroacupuncture groups these parameters were decreased (P＜0.01),with a significant difference between the two treatment groups (P＜0.01).Conclusion: Electroacupuncture treatment inhibited apoptosis and provided neuroprotection.     

慢性粒细胞白血病端粒酶的相关研究

目的：对慢性粒细胞白血病端粒酶进行研究,采用 PCR －ELISA 半定量法测定端粒酶活性,同时观察端粒酶活性变化的相关性。方法：选取15例慢性粒细胞白血病患者骨髓标本和15例正常骨髓（取自非肿瘤病人需行骨髓检查者）样本分为慢性粒细胞白血病组（CML 组）和正常对照组,采用 PCR －ELISA 法来测定CML 组和正常对照组的端粒酶活性。结果：正常对照组端粒酶具有微弱的活性,而 CML 组则具有较强的活性,且端粒酶活性 CML 组高于正常对照组,两者差异性非常显著。其中,CML 组男性与女性组间端粒酶活性差异无统计学意义。结论：正常组端粒酶具有微弱的活性,而 CML 组则具有较强的活性。端粒酶活性可能反映了一个高度增生的状态,是一种新的肿瘤标记物,与肿瘤发生密切相关。     

Parasitic loranthus from Loranthaceae rather than Viscaceae potently inhibits fatty acid synthase and reduces body weight in mice

AIM OF THE STUDY: Our previous results have shown one species of parasitic loranthus (Taxillus chinensis (DC.) Dancer) exhibits potent inhibition on fatty acid synthase (FAS) that is proposed to be a potential therapeutic target for treatment of obesity. However, the medicinal parasitic loranthus come from tens of plants of two families, the Loranthaceae and the Viscaceae. This study was carried out to figure out whether these parasitic loranthus from the two families have similar inhibitory ability on FAS, and whether the parasitic loranthus with potent inhibitory ability on FAS significantly reduce body weight of animal. MATERIALS AND METHODS: CD-1 mice were used to test the effects of samples on their body weight and food intake in 20 days. The reversible and irreversible inhibition on FAS was assayed to study the inhibitory ability of sixteen different medicinal plants from these two families, which were collected in nature. RESULTS: The reversible inhibitory ability of the extracts from the Loranthaceae was nearly 400-fold stronger than that from the Viscaceae. The species from the genus Taxillus Tieghem showed the best effect on FAS in both reversible and irreversible inhibition. Moreover, the difference in host plants did not affect markedly on the inhibitory ability of parasitic loranthus. The medicinal herb with high inhibitory ability on FAS significantly reduced the body weight and food intake of mice by oral administration. CONCLUSIONS: The medicinal herbs from the family Loranthaceae, rather than those from the family Viscaceae, should be suitable to apply as botanical sources of parasitic loranthus for weight control. The herbs from genus Taxillus Tieghem are the best.     

不完全型P450 17α酶缺乏症六例报道及分析

目的 探讨不完全型P45017α酶（17α羟化爵每／17,20裂解酶）缺乏症（17OHD）的临床特征、鉴别诊断和处理方法。方法 回顾性分析北京协和医院6例不完全型17OHD的临床特征,并通过文献复习讨论该病的发病机制和典型的临床特征。结果 6例中4例为46,XX不完全型17OHD患者,其临床特征包括女性表型、不同程度的乳房发育、阴毛少、月经稀发或继发闭经、反复发作的卵巢囊肿、性腺功能低下伴持续性血清孕酮和（或）17α羟孕酮水平升高、合并或不合并低钾性高血压;另2例为46,XY不完全型17OHD患者,伴有不同程度的外生殖器性别不清和低钾性高血压。结论 不完全型17OHD是一种极为罕见的先天性甾体合成酶缺乏,临床上出现月经异常、性发育幼稚、反复发作的卵巢囊肿或外生殖器性别不清时应考虑此病。     

Tumor lymphangiogenesis and lymphangiogenic growth factors

Recent studies have revealed that malignant tumors can actively induce the formation of new lymphatic vessels and metastasize through the lymphatic system. Tumor-induced lymphangiogenesis driven by tumors expressed lymphangiogenic growth factors such as VEGF family, fibroblast growth factor 2 (FGF-2), angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2), and platelet-derived growth factors (PDGFs) is correlated with lymph node metastasis in experimental cancer models and in several types of human cancers. Tumor- induced lymphangiogenesis has now been firmly established as a novel mechanism for cancer progression and lymph node metastasis. Recent studies indicate that blockade of the lymphangiogenic growth factors pathway inhibits tumor spread to lymph nodes and likely beyond. The potential effects of most of these newly identified lymphatic growth factors on tumor-induced lymphangiogenesis and lymph node metastasis remain to be further investigated. A number of questions remain to be answered concerning the potential efficacy of targeting at tumor-induced lymphangiogenesis for inhibiting tumor spread to lymph nodes.