子宫内膜异位症患者治疗前后血清TSGF和血浆VEGF水平的临床意义

目的：探讨了子宫内膜异位症患者治疗前后血清TSGF和血浆VEGF水平的变化及临床意义。方法：应用ELISA法测定31例子宫内膜异位症患者血浆VEGF含量,光电比色法测定TSGF含量,并与30名正常健康人作比较。结果：子宫内膜异位症患者在治疗前血清TSGF和血浆VEGF水平非常显著地高于正常人组（P＞0．01）。经中西医结合治疗后3个月,则与正常人组比较无显著性差异（P〉0．05）。结论：子宫内膜异位症的发生与发展与血清TSGF和血浆VEGF水平密切相关。     

幽门下动脉的解剖观测及临床意义

目的：为幽门毗邻部位病变的诊治或手术提供解剖学资料。方法：采用红色乳胶液经动脉灌注和防腐处理的尸体标本，解剖观测幽门下动脉的起始类型及分支分布情况。结果：（1）幽门下动脉主要起源于胃十二指肠动脉（51．6％）或胃网膜右动脉（29．0％）；（2）幽门下动脉双干起始占55．0％，单干起始占45．0％；（3）幽门下动脉与胃网膜右动脉在幽门下端构成浆膜下吻合。结论：在胃手术处理胃网膜右动脉时，应在幽门下动脉起点的远端结扎胃网膜右动脉，以防止幽门附近残胃血供匮乏。     

IL-4对DC产生IL-12影响的研究

为了研究白细胞介素 4 (IL 4 )对树突状细胞 (dendriticcell,DC )产生白细胞介素 1 2 (IL 1 2 )p70的调节作用及其机制 ,将小鼠骨髓细胞在含GM CSF和IL 4的培养液中培养 6d ,加入成熟诱导剂脂多糖 (LPS )或TNF α、CpG ODN继续培养 2d以获得成熟DC。流式细胞仪检测DC表面CD80和MHCII类分子 ,混合淋巴细胞反应检测DC促进同种异体T细胞增殖的能力 ,ELISA法检测不同诱导剂诱导DC产生IL 1 2p70的能力 ,RT PCR和荧光定量PCR检测IL 1 2p35和p4 0mRNA的表达及其变化。结果显示小鼠骨髓细胞在含GM CSF、IL 4和成熟诱导剂的培养液中培养后可以获得成熟的DC ,成熟DC高表达CD80和MHCII类分子 ,具有较强的刺激同种异体T细胞增殖的能力 ,LPS、CpG ODN、TNF α、PolyI:C在促进DC成熟的同时能诱导DC产生有活性的IL 1 2 ,IL 4对IL 1 2的产生具有明显的促进作用 ,RT PCR和荧光定量PCR结果显示LPS诱导IL 1 2产生以及IL 4对其的促进作用与IL 1 2p35基因的转录水平增高有关     

Terminal Schwann cells guide the reinnervation of muscle after nerve injury

Schwann cells and axons labeled by transgene-encoded, fluorescent proteins can be repeatedly imaged in living mice to observe the reinnervation of neuromuscular junctions. Axons typically return to denervated junctions by growing along Schwann cells contained in the old nerve sheaths or "Schwann cell tubes". These axons then commonly "escape" the synaptic sites by growing along the Schwann cell processes extended during the period of denervation. These "escaped fibers" grow to innervate adjacent synaptic sites along Schwann cells bridging these sites. Within the synaptic site, Schwann cells, originally positioned above the synaptic site continue to cover the acetylcholine receptors (AChRs) immediately following denervation, but gradually vacate portions of this site. When regenerating axons return, they first deploy along the Schwann cells and ignore sites of AChRs vacated by Schwann cells. In many cases these vacated sites are never reinnervated and are ultimately lost. Following partial denervation, Schwann cells grow in an apparently tropic fashion from denervated to nearby innervated synaptic sites and serve as the substrates for nerve sprouting. These experiments show that Schwann cells provide pathways that stimulate axon growth and insure the rapid reinnervation of denervated or partially denervated muscles.     

Hydroxyethyl starch inhibits NF-kappaB activation and prevents the expression of inflammatory mediators in endotoxic rats

Hydroxyethyl starch (HES) has been shown to be beneficial in several inflammatory situations, but the mechanisms are unclear. The present study tested the hypothesis that HES has effects on nuclear factor kappa B (NF-kappaB) activation and the expression of inflammatory mediators induced by lipopolysaccharide. Sepsis was induced in male Wistar rats by injection of lipopolysaccharide (LPS, 6 mg/kg, i.p.). At 1 min after the LPS challenge, HES was infused via the right external jugular vein at the following doses: 3.75, 7.5, 15, or 30 ml/kg. NF-kappaB activation in peripheral blood mononuclear cells and neutrophils, plasma concentrations of tumor necrosis factor (TNF)-alpha, cytokine-induced neutrophil chemoattractant (CINC), expression of CD11b on the blood neutrophil cell surface, and neutrophil sequestration in multiple organs were examined 2 or 4 hr after the LPS challenge. Treatment of rats with HES (3.75 and 7.5 ml/kg) prevented LPS-induced NF-kappaB activation, and inhibited, in a dose-related manner, LPS-induced TNF-alpha and CINC expression. The 4 graded doses of HES decreased CD11b expression in a dose-dependent manner. HES significantly reduced neutrophil sequestration in lung, heart, and liver. These results suggest that HES has an anti-inflammatory effect in endotoxic rats. This effect is mediated by inhibition in the production pathways for inflammatory mediators, including NF-kappaB activation.     

Enhancement of the immunogenicity of an infectious bronchitis virus DNA vaccine by a bicistronic plasmid encoding nucleocapsid protein and interleukin-2

A DNA vaccine against infectious bronchitis virus (IBV) can induce specific humoral and cell-mediated immunity. However, compared to conventional vaccines, DNA vaccines usually induce poor antibody responses. To develop a more potent IBV DNA vaccine formulations, a monocistronic vector encoding the nucleocapsid protein of IBV and a bicistronic vector separately encoding the nucleocapsid protein and immune-stimulatory interleukin-2 were constructed. When the DNA vaccines were administered to the quadriceps muscle of chickens, the induced humoral and cellular responses were evaluated. There was a significant difference in ELISA antibody levels elicited by either monocistronic or bicistronic DNA vaccines. The percentage of CD3+, CD3+CD8+ and CD3+CD4+ subgroups of peripheral blood T-lymphocytes in chickens immunized with bicistronic DNA vaccine were higher than those in chickens immunized with monocistronic DNA vaccine. When chickens were challenged with a virulent strain of IBV, the protective efficacy could be enhanced significantly after immunization with bicistronic DNA vaccine. These results demonstrated that bicistronic DNA vaccine is an effective approach to increase IBV DNA vaccine immunogenicity.     

青少年学生体像烦恼、孤独、应对方式的相关研究

目的了解青少年学生的体像烦恼、孤独及应对方式三者的关系。方法采用调查问卷对293名中学生进行调查,收集体像烦恼、孤独和应对方式的有关指标及身高、体重等人口学资料。结果1体像烦恼与消极应对方式相关显著,与孤独相关不显著。2孤独与积极应对方式相关显著,与消极应对方式相关不显著。3积极应对方式、性别烦恼与BMI均相关显著。结论有体像烦恼和孤独的青少年学生倾向于采用消极应对方式,偏胖或偏瘦的青少年学生对自己的性别不满意,这些在高一年级学生身上表现得比较明显。     

Biphasic activation of apoptosis signal-regulating kinase 1-stress-activated protein kinase 1-c-Jun N-terminal protein kinase pathway is selectively mediated by Ca2+-permeable alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors involving oxidative stress following brain ischemia in rat hippocampus

Stress-activated protein kinase/extracellular signal-regulated kinase-1 (SEK1/MKK4) was examined in a rat model of global brain ischemia. Western blot assay showed that SEK1 activation was biphasic in CA1 but not CA3/dentate gyrus. The second activation peak (3 days after ischemia) was prevented by pretreatment with l-naphthyl acetyl spermine (Naspm), a channel blocker of Ca(2+)-permeable alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptors, or N-acetylcysteine (NAC), a free radical scavenger. Concomitantly, the late activation of apoptosis signal-regulating kinase 1 (ASK1) and c-Jun N-terminal protein kinase (JNK) was also prevented by Naspm or NAC. Moreover, phospho-SEK1 and phospho-JNK co-immunoprecipitated with ASK1 and the bindings peaked at 3 days of reperfusion. Together with previous results, these findings indicate that Ca(2+)-permeable AMPA receptors are important routes to mediate the late activation of ASK1-SEK1-JNK pathway involving oxidative stress in hippocampal CA1 region after ischemia.     

蛋白质组学在心血管研究中的应用

随着基因组数据库的不断完善和蛋白质组学技术的不断发展，已有越来越多的研究者将蛋白质组学运用到心血管疾病研究中，即运用2—DE、质谱等手段来观察心肌缺血、高血压、心肌肥大、心衰、心肌梗塞导致的心肌细胞蛋白质表达水平和翻译后修饰(PTM)的改变，对这些急慢性心血管疾病的分子机制给予独特的解释。与此同时，各种心血管疾病模型相关蛋白质组数据库也在建设之中，这将大大方便更多的蛋白质组研究。本文综述了近年来使用蛋白质组学在心血管研究中所取得的进展。