植物基除臭剂的配方设计及净化效果的试验研究

目的:通过研究植物基除臭剂的配方,探讨其净化效果。方法:采用市售植物复配精油为基底,三甘醇为溶剂,加入一定比例的乳化剂、助剂和去离子水配制植物基除臭剂。乳化剂选用比较常见的10种表面活性剂进行冷储和热储对比试验,助剂选用β-环糊精,通过三因素三水平正交试验确定配方中植物精油、乳化剂和助剂含量。通过气相色谱质谱分析除臭剂...     

PARP1单核苷酸多态性对晚期胃癌奥沙利铂化疗反应及预后的影响

目的探讨多聚腺苷二磷酸核糖聚合酶(PARP-1)单核苷酸多态性(SNP)与晚期胃癌患者化疗反应及生存时间的关联性。方法选择2016年5月至2019年12月于苏州科技城医院肿瘤内科收治的161例晚期胃癌患者,给予奥沙利铂联合替吉奥化疗,化疗前抽空腹静脉血5 ml用于提取基因组DNA。TaqMan探针法鉴别PARP1基因rs907187C/G、rs1805414T/C和rs1136410T/C多态性位点的基因型,分析各多态性对化疗客观反应率(ORR)及总生存期(OS)的影响。结果 rs907187C/G多态性与化疗敏感性存在明显关联,变异等位基因G携带者化疗ORR显著升高,CC、CG、GG基因型分别为23.2%、42.2%、53.7%(P=0.007)。杂合基因型CG化疗ORR为CC基因型的2.362倍(P=0.035);纯合变异基因型GG化疗ORR为CC基因型的3.812倍(P=0.003);CG+GG基因型化疗ORR为CC基因型的2.854倍(P=0.005)。Kaplan-Meier分析显示rs1136410T/C多态性与患者OS显著相关,变异等位基因C携带者中位OS明显延长,TT、...     

结节性脆发症1例

报告1例结节性脆发症。患者女,27岁,头发白色结节、易断17年。皮肤专科情况：头发稀少,发质干枯,见较多沿毛干密集分布的细小颗粒样白色结节,轻拉头发易断裂。皮肤镜检查：病变区呈中间粗两端细的白色结节状改变。光镜检查：毛小皮不连续,干燥无光泽,较多断裂的丝状纤维向外展开,像两把“扫帚”或“漆刷”末端相互插入。扫描电镜检查：病变中央毛小皮消失,皮质层纤维断裂,粗细不均,部分向外展开或卷曲。诊断：结节性脆发症。     

直腰旋转平推法治疗腰椎间盘突出症效果分析

目的:观察直腰旋转平推法治疗腰椎间盘突出症的临床疗效。方法:569例腰椎间盘突出症患者,随机分为直腰旋转平推法治疗组301例,常规推拿治疗组268例。两组均每日治疗一次,10次为一疗程,1个疗程后进行临床疗效观察。结果:治疗组疗效明显优于对照组(P<0.05)。结论:直腰旋转平推法治疗腰椎间盘突出症疗效确切,优于常规推拿治疗。     

大黄及其蒽醌对自身免疫性疾病治疗的研究进展

大黄始载于《神农本草经》,药理活性广泛.自身免疫性疾病是一类因机体免疫耐受功能受损而致免疫细胞及其成分对自身组织结构和功能破坏的疾病,包括动脉粥样硬化、多发性硬化、痛风、类风湿性关节炎、自身免疫性甲状腺炎、溃疡性结肠炎、1型糖尿病、IgA肾病等.近年临床和实验研究显示,大黄及其蒽醌类成分对自身免疫性疾病具有良好的治疗潜...     

Exosomes Derived from Glioma Cells under Hypoxia Promote Angiogenesis through Up-regulated Exosomal Connexin 43

Glioblastoma multiform (GBM) is a highly aggressive primary brain tumor. Exosomes derived from glioma cells under a hypoxic microenvironment play an important role in tumor biology including metastasis, angiogenesis and chemoresistance. However, the underlying mechanisms remain to be elucidated. In this study, we aimed to explore the role of connexin 43 on exosomal uptake and angiogenesis in glioma under hypoxia. U251 cells were exposed to 3% oxygen to achieve hypoxia, and the expression levels of HIF-1α and Cx43, involved in the colony formation and proliferation of cells were assessed. Exosomes were isolated by differential velocity centrifugation from U251 cells under normoxia and hypoxia (Nor-Exos and Hypo-Exos), respectively. Immunofluorescence staining, along with assays for CCK-8, tube formation and wound healing along with a transwell assay were conducted to profile exosomal uptake, proliferation, tube formation, migration and invasion of HUVECs, respectively. Our results revealed that Hypoxia significantly up-regulated the expression of HIF-1α in U251 cells as well as promoting proliferation and colony number. Hypoxia also increased the level of Cx43 in U251 cells and in the exosomes secreted. The uptake of Dio-stained Hypo-Exos by HUVECs was greater than that of Nor-Exos, and inhibition of Cx43 by ^37,43gap27 or lenti-Cx43-shRNA efficiently prevented the uptake of Hypo-Exos by recipient endothelial cells. In addition, the proliferation and total loops of HUVECs were remarkably increased at 24 h, 48 h, and 10 h after Hypo-Exos, respectively. Notably, ^37,43gap27, a specific Cx-mimetic peptide blocker of Cx37 and Cx43, efficiently alleviated Hypo-Exos-induced proliferation and tube formation by HUVECs. Finally, ^37,43gap27 also significantly attenuated Hypo-Exos-induced migration and invasion of HUVECs. These findings demonstrate that exosomal Cx43 contributes to glioma angiogenesis mediated by Hypo-Exos, and suggests that exosomal Cx43 might serve as a potential therapeutic target for glioblastoma.     

ST 段抬高心肌梗死急诊冠脉介入中室颤发生的分析

目的:分析ST 段抬高心肌梗死在急诊冠脉介入中发生室颤(VF)的临床特征和冠脉造影特点.方法:对50例接受急诊冠脉介入治疗的ST段抬高心肌梗死患者进行回顾性研究,根据术中有无室颤将患者分为两组,其中9例发生心室颤动(VF组),41例患者没有发生心室颤动(无VF组).比较两组的临床特征与冠脉造影的差异.结果:两组患者的基线特征相似.VF组冠脉内溶栓占33.3%,无VF组冠脉内溶栓2.4%,两组统计学上有显著性差异(P<0.05).VF组三支病变占77.7%,无VF组占33.3%,两组统计学上有显著性差异(P<0.05).结论:(1)在ST段抬高的心梗患者在急诊冠脉介入时室颤发生可能与冠脉内急性闭塞处血栓负荷有关.(2)在ST段抬高的心梗患者在急诊冠脉介入时室颤发生与冠脉病变严重程度有关.     

山茱萸化学成分与气象因子相关性分析

目的:对不同产地山茱萸中化学成分进行含量测定,明确不同产地气象因子对化学成分的影响及其相关性.方法:对河南、陕西及浙江3个主产区14个产地山茱萸药材中熊果酸、马钱苷、总多糖、有机酸、鞣质、水溶性浸出物及醇溶性浸出物进行含量测定,运用数理统计学软件(PASW STATISTICS 18)各化学成分指标与产地气象因子进行相关性分析.结果:山茱萸药材中马钱苷、醇浸物、总有机酸、鞣质的含量与产地气象因子没有显著相关性;熊果酸含量与产地日照呈现负相关,多糖含量与产地年日照时数和年均温度呈正相关;水浸物含量与产地年均温度成正相关.结论:明确了山茱萸化学成分与气象因子之间的相关性,为培育质优、高产的山茱萸提供理论基础,同时为山茱萸的GAP生产提供理论支持.     

基于α-葡萄糖苷酶抑制活性评价桑叶多组分药效相互作用研究

采用柱色谱法对桑叶提取物中黄酮、生物碱、多糖组分进行富集分离;采用葡萄糖氧化酶法,利用蔗糖为底物,以α-葡萄糖苷酶抑制模型对桑叶多组分进行评价;采用等效线法、周-特氏联合指数法和等辐射分析法评价2组分间的药效相互作用及量效特点,为揭示桑叶降血糖的作用机制提供科学依据。成分分析表明,桑叶提取物中,黄酮质量分数为5.3%,有机酚酸质量分数为10.8%,1-脱氧野尻霉素(DNJ)质量分数为39.4%,多糖质量分数为18.9%。活性评价表明,桑叶黄酮、生物碱、多糖组分均具有显著的α-葡萄糖苷酶抑制活性,其抑制率随各组分浓度的增加而升高,3个组分中桑叶生物碱抑制活性最强。桑叶生物碱与桑叶黄酮配伍、桑叶生物碱与桑叶多糖配伍均表现出协同增效作用,但多糖与黄酮组配伍未见明显的协同作用,从而证实了桑叶多组分调节血糖的相互作用,为桑叶多组分降血糖物质基础与作用机制的揭示提供了科学依据。