布地奈德对哮喘大鼠内源性H2S、CSE、CBS 体系的调节机制

目的：观察布地奈德对哮喘大鼠血浆硫化氢（H2S）含量和胱硫醚-γ-裂解酶（CSE）、胱硫醚-β-合成酶(CBS) mRNA的表达,探讨内源性H2S、CSE、CBS体系在哮喘发病机制中的作用。方法：Sprague-Dawley大鼠30只随机分成对照组、哮喘组、布地奈德组,每组10只。用鸡卵白蛋白（OVA）致敏与激发建立大鼠哮喘模型。分光光度法测定血浆H2S的含量,RT-PCR 法测定肺组织CSE、CBS mRNA的表达水平。结果：哮喘组血浆H2S的含量（61±16 μmol/L）显著低于对照组（84±15 μmol/L）（P0.05）。哮喘组肺组织CSE mRNA和CBS mRNA的表达水平显著低于对照组（P<0.01）,布地奈德组CSE mRNA和CBS mRNA表达显著低于对照组但高于哮喘组（均P<0.01）。相关性分析结果显示血浆H2S含量和支气管肺泡灌洗液(BALF)中炎症细胞总数表达呈显著负相关(n=30, r=-0.549,P<0.01)。结论：哮喘大鼠H2S、CSE、CBS的表达下降,它们的表达下降可能促进了炎症细胞在气道中的聚集,布地奈德改善哮喘炎症的机制可能部分通过内源性H2S、CSE、CBS体系起作用。［中国当代儿科杂志,2010,12（8）：654-657］     

强脉冲光照射兔皮肤后的组织学观察

目的：观察强脉冲光照射兔皮肤后的组织学变化，为临床提供参考信息。方法：用34J／cm^2的强脉冲光对10只兔四个部位分别进行1．2、3、4次照射，术后第1、7、15、30、60、90天行切片观察。结果：一定能量的IPL能治疗光老化，并不引起严重炎症反应；IPL可使表皮层增厚，引起的胶原纤维增生可持续3个月以上。结论：光子嫩肤术可安全有效的治疗光老化，其效果可超过3个月，但它也是一种创伤性刺激。     

Over-expression of NYGGF4 inhibits glucose transport in 3T3-L1 adipocytes via attenuated phosphorylation of IRS-1 and Akt

Aim： NYGGF4 is a novel gene that is abundantly expressed in the adipose tissue of obese patients. The purpose of this study was to investigate the effects of NYGGF4 on basal and insulin-stimulated glucose uptake in mature 3T3-L1 adipocytes and to understand the underlying mechanisms.
Methods： 3T3-L1 preadipocytes transfected with either an empty expression vector （pcDNA3.1Myc/His B） or an NYGGF4 expression vector were differentiated into mature adipocytes. Glucose uptake was determined by measuring 2-deoxy-D- [^3H]glucose uptake into the adipocytes. Immunoblotting was performed to detect the translocation of insulin-sensitive glucose transporter 4 （GLUT4）. Immunoblotting also was used to measure the phosphorylation and total protein contents of insulin signaling proteins such as the insulin receptor （IR）, insulin receptor substrate （IRS）-I, Akt, ERK1/2, p38, and JNK.
Results： NYGGF4 over-expression in 3T3-L1 adipocytes reduced insulin-stimulated glucose uptake and impaired insulinstimulated GLUT4 translocation. It also diminished insulin-stimulated tyrosine phosphorylation of IRS-1 and serine phosphorylation of Akt without affecting the phosphorylation of 1R, ERK1/2, p38, and JNK.
Conclusion： NYGGF4 regulates the functions of IRS-1 and Akt, decreases GLUT4 translocation and reduces glucose uptake in response to insulin. These observations highlight the potential role of NYGGP 4 in glucose homeostasis and possibly in the pathogenesis of obesity.     

围手术期化疗加外科手术治疗晚期胃癌

Objective To investigate the feasibility and safety of designed perioperative surgical treatment for advanced gastric cancer.Method From March 2006 to Sep.2009,24 patients with advanced gastric cancer were enrolled for this study,of which 14 cases were graded as Ⅳ M0,10 as Ⅳ M1,including liver metastasis in 5 cases,peritoneal seeding in 4 and lung metastasis in 1.All patients received 2-4 cycles of chemotherapy of PCF or ECF regimen,followed by intended surgical resection and postoperative 2-3 cycles of adjuvant chemotherapy of the same regimen as used during the preoperative course.Result Seven Cases abandoned surgical therapy,a total of 17 cases completed preoperative chemotherapy,surgical resection and postoperative chemotherapy.R0 resection was achieved in 16 cases(94%).There was no perioperative mortality,and total clinical response rate was 75%,overall pathological response rate was 82%.In 59% surgical cases (10 cases) serum CEA and CA199 has returned to normal;In 71%patients tumor pathological stage degraded at the cost of Ⅲ-Ⅳ grade of neutropenia,thrombocytopenia,anemia,severe nausea and vomiting in 79%,8%,13%,and 54% cases respectively.Fever was encountered in 2 cases with grade Ⅲ-Ⅳ neutropenia.Conclusion Designed perioperative chemotherapy regimen renders advanced gastric carcinoma patients operable at the cost of acceptable complications.     

锁定加压接骨板治疗肱骨中下段骨折

2003年8月-2006年7月，我科使用锁定加压接骨板内固定治疗27例肱骨中下段骨折，疗效较好。     

医疗设备安全指数受电能质量影响的研究

目的：研究电能质量对医疗设备安全指数的影响。方法：以循证医学模式为指导。对6类共100台分属医院各科室的、临床使用率较高的A类设备进行历史性列队跟踪调查，详细记录每台设备每次故障的情况．对同一时段内电能质量监测的结果进行比较、分析，鉴别，筛选出故障性质归属，取得相关数据。结果：受电能质量影响而引发的356次故障中，电源部分的故障次数为159次，控制部分的故障次数为157次，信号通道的故障次数为40次。结论：电力污染使电能质量下降，导致医疗设备故障率升高。安全指数下降给医疗设备的安全运行造成严重的负面影响，医学工程技术部门对此应高度警惕。     

Fresenius4008B血透机水路图原理分析

本文详细分析了Fresenius4008B血透机水路图的工作原理以及测试参数，充分说明了水路图中每个元器件的工作状态。     

家庭锡箔加工作坊对儿童健康的影响

目的对兰溪市、浦江县锡箔加工家庭作坊的儿童进行监测,探讨乡镇环境铅污染对当地儿童健康的影响.方法在污染区46名和对照区32名儿童中进行血铅测定,并分别进行了WISC-R儿童智能发育的检查测试.结果污染区儿童血铅明显高于对照组儿童,血铅水平与儿童韦氏智商存在负相关关系.结论家庭作坊的锡箔加工严重污染了当地儿童的生长环境,对儿童的智能发育造成一定的影响.