Mechanisms of polyploidization of mouse cardiomyocytes

Most ventricular cardiomyocytes in mice aged 5–6 days are polyploid cells. By this time 60% of the cardiomyocytes have become binuclear and a further 10% have become mononuclear polyploid cells. Binuclear cardiomyocytes are formed as a result of acytokinetic mitosis, mononuclear tetraploid cells as a result of termination of mitosis in the initial phases.Laboratory of Cytology, N. K. Kol'tsov Institute of Developmental Biology, Academy of Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR A. P. Avtsyn.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 89, No. 2, pp. 219–222, February, 1980.     

The involvement of glutamatergic transmission in the mechanism of movement disorders induced by reversive rotation of white mice

The ability of the selective non-competitive NMDA receptor blocker MK-801 and a series of new glutamate antagonists—the adamantane derivatives IEM-1754 and IEM-1857 and phencyclidine (IEM-1925)—to prevent movement disorders induced by reversive rotation in mice was studied. I.p. MK-801 at a dose of 0.15 ml and IEM-1754 at a dose of 5.0 mg/kg prevented the development of akinesia in response to reversive rotation, as effectively as scopolamine, a known agent which provides effective prophylaxis for movement diseases. IEM-1857, the quaternary analog of IEM-1754, was not effective. IEM-1925 significantly increased the responses of mice to reversive rotation, possibly because of its high activity in relation to other subtypes of glutamate receptors. These data provide evidence for the involvement of glutamatergic transmission in the mechanism of movement disorders of vestibular origin. Translated from Rossiiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 85, No. 4, pp. 497–501, May, 1999.     

IL-18在体外培养系统中诱导肿瘤快速杀伤效应及肿瘤特异性CTL

目的应用rhlL-18在体外培养系统(Coculture system in vitro，CCs)中诱导快速肿瘤杀伤效应及诱导肿瘤特异性细胞毒性T淋巴细胞(Cytotoxic T Lymphocyte，CTL)。方法 采用StemSep^TM免疫磁性细胞分离法分离人外周血NK细胞、T细胞及树突细胞(DCs)，流式细胞仪分析细胞表型，125I-UdR标记的细胞毒实验检测杀伤活性，ELISA方法检测IFN-7的产生量。结果 在CCs中，rhIL-18诱导出快速肿瘤杀伤效应，这种杀伤效应无抗原特异性、不受MHC限制，DCs和T细胞的存在与否对其无明显影响。在同一培养系统中，肿瘤抗原存在的条件下，96h后，rhIL-18能够诱导并促进CTL介导的肿瘤特异性杀伤效应。结论 rhIL-18能够在体外培养系统中相继诱导肿瘤快速杀伤效应及肿瘤特异性CTL。     

Certain characteristics of myocardial contractility of isovolumic dog heart at randomly variable heart rhythm

Summary The relationship heart rate-left ventricular pressure was investigated in the isolated canine heart perfused with constant pressure at different preloads. Rhythmical stimulation was performed with constant stimulus interval duration and with stimulus intervals randomly changed near the average value (150–200 stimuli in series).Correlation and dispersion function analysis show that rhythm dispersion had a negative inotropic effect which was independent of the preload of the ventricle in the range of 120–180 beat/min, but this dependence occurred with low rates of stimulation.This method is proposed for the assessment of contractility under conditions of heart rate variations (physiological and pathological arrhythmias).     

抑酸抗菌药联合治疗消化性溃疡的疗效观察

68例经胃镜证实的消化性溃疡患者,随机分成两组,分别接受常规抑酸护膜及抑酸抗菌联合治疗。服药法：常规治疗组为雷尼替丁0．15g,2次／d,三钾二橼络合铋120mg,4次／d,抗菌治疗组为三钾二橼络合铋120mg,4次／d,甲硝唑0．2g,4次／d,阿莫西林250mg,4次／d,均连服4周为一疗程,结果两组治愈率分别为73％（22／30）和87％（33／38）,而追踪观察治愈者1年复发率却分别为68％（15／22）和3％（1／33）。结果表明应用抑酸抗菌联合治疗消化性溃疡,具有症状缓解迅速、逼反应少,价格低、病人依从性好等优点,是一种较理想的消化性溃疡的治疗方案。     

Kubelka-Munk模型下的兔血管对氦-氖激光的散射与吸收特性

测量了兔动脉和静脉对 He-Ne激光的反射和透射传输特性。实验采用两积分球系统及波长为632.8nm的He-Ne激光器,并根据测量数据及采用 Kubelka-Munk模型分析和计算了兔动脉和静脉组织对该长激光的吸收系数、散射系数及总的光强I（x）及前向散射通量i(x）和后向散射通量j(x）随厚度的变化情况。结果表明,兔动脉和静脉的漫反射率和透射率有明显差别,而且,动脉对激光的吸收系数明显较静脉的要小,而动脉对激光的散射系数却明显较静脉的要大,在动脉和静脉组织中总的光强I（x）及前向散射通量i(x）和后向散射通量j(x）随组织厚度的变化情况也有明显的区别。     

灌洗法一期叻除吻合术在左侧梗阻性结肠癌中的应用

目的：探讨左半结肠癌并梗阻一期切除吻合的可行性。方法：术中灌洗肠内容物,术后吻合口减压。结果：1995年至1999年所遇左侧梗阻性大肠癌中选择5例用此方法吻合成功。结论：左侧梗阻性大肠癌,只要掌握好手术指针。术中充分灌洗肠内容物,术后扩肛可一期切除吻合。     

大鼠去势后胸腺变化的组织学及超微结构的观察

目的：探讨去势对大鼠胸腺淋巴细胞超微结构与细胞功能的影响。方法：分别于去势大鼠术后2周、4周、6周、8周后,取胸腺组织进行HE染色和透射电镜观察。结果：实验表明去势导致胸腺淋巴细胞结构坏死,或者细胞器严重受损。结论：去势后胸腺淋巴细胞形态和功能均受到损害。