脂蛋白脂酶活化剂NO-1886抑制糖尿病兔动脉粥样硬化的形成

目的　合成化合物NO 1886 ,一种脂蛋白脂酶活化观察脂蛋白脂酶活化剂是否降低高脂 /高蔗糖饲料诱发的糖尿病新西兰兔的血浆葡萄糖并减轻其动脉粥样硬化。方法　给予高脂 /高蔗糖饲料升高新西兰兔血浆总胆固醇 ,甘油三酯和葡萄糖及降低高密度脂蛋白胆固醇而导致动脉粥样硬化。饲料中加入 1 0 %NO 1886进行治疗观察。分别在 0、4、8、12、16、2 0及 2 4wk从禁食过夜的兔耳静脉抽取血样测定葡萄糖和脂质水平。第 2 4周末 ,处死动物 ,分离主动脉 ,经苏丹Ⅳ染色固定脂质后 ,计算脂纹病变面积。结果　应用NO 1886后 ,实验动物血浆葡萄糖 ,总胆固醇和甘油三酯降低 ,高密度脂蛋白胆固醇增加。对动脉粥样硬化的发展具有抑制作用。结论　NO 1886不仅可改善脂质紊乱 ,而且可降低血浆葡萄糖 ,减轻糖尿病兔动脉粥样硬化     

Inhibitory effect of topiramate on Lewis lung carcinoma metastasis and its relation with AQP1 water channel

AIM: To study the effect of topiramate on tumor metastasis and its relation with aquaporin 1 (AQP1) water channel. METHODS: Lewis lung carcinoma metastatic model was used to determine the effect of topiramate on tumor growth and metastasis. Colorimetric estimation was used to investigate the action of topiramate on carbonic anhydrase (CA) activity. Western blotting and immunohistochemical analysis were used to study the influence of topiramate on AQP1 water channel expression in lungs or tumor tissues of mice bearing Lewis lung carcinoma.     

聚酰胺胺树形化合物基因载体的研究进展

聚酰胺胺阳离子聚合物是一类新型高分子树形化合物,在药物递送、基因转染、疾病诊断等领域有广泛的应用。因其具有无免疫原性、低毒性等优点,聚酰胺胺树形化合物越来越受到人们的关注,在基因治疗领域发挥着重要的作用。本文对目前聚酰胺胺树形化合物作为基因载体的研究进展进行综述。     

提高异位甲状腺诊断符合率的方法

目的 :探讨提高异位甲状腺诊断符合率的方法。方法 :对 11例异位甲状腺的临床资料作回顾性分析。结果 :11例异位甲状腺患者都经99m锝 (99mTc)扫描甲状腺扫描而确诊 ;甲状腺功能低下者 (T3 、T4降低 ,TSH升高 ) 7例 ;行B超和穿刺细胞学检查 3例 ,结果与99mTc扫描一致。结论 :99mTc甲状腺扫描是诊断异位甲状腺的最好方法 ;细胞学检查是其确诊方法 ;B超是诊断该病有用的辅助方法     

抗眼组织纤维化药物的研究进展

纤维化是许多眼科手术失败的主要原因之一，应用抗纤维化药物能提高手术的成功率。如：5—氟尿嘧啶、丝裂霉素C、组织型纤溶酶原激活剂等已用于实验和临床研究，本文重点提出转化生长因子—β抑制剂核心蛋白多糖(Decorin)有可能作为抗眼组织纤维化的药物。     

高度近视眼LASIK前后视网膜神经纤维层厚度的HRT测量

目的 探讨准分子激光原位角膜磨镶术(LASIK)对高度近视眼视网膜神经纤维层厚度的影响.方法 用海德堡视网膜断层扫描仪(HRT)黄斑水肿分析软件(MEM)对施行准分子激光原位角膜磨镶术8例(16只眼),近视度数为-6.75～-11.25 D(-9.75±1.75 D)的高度近视眼患者的黄斑视网膜信号宽度进行测量.检测时间为术前、术后第1、3和7 d.检测部位为黄斑中央及距离黄斑中心凹500 μm的视网膜神经纤维层.结果 准分子激光原位角膜磨镶术术前、术后第1、3和7 d视网膜信号宽度差异有显著意义(P＜0.05).结论 准分子激光原位角膜磨镶术对高度近视视网膜神经纤维层的厚度有影响.     

IFN‐α/β‐mediated NK2R expression is related to the malignancy of colon cancer cells

Neurokinin 2 receptor (NK2R), a G protein‐coupled receptor for neurokinin A (NKA), a tachykinin family member, regulates various physiological functions including pain response, relaxation of smooth muscle, dilation of blood vessels, and vascular permeability. However, the precise role and regulation of NK2R expression in cancer cells have not been fully elucidated. In this study, we found that high NK2R gene expression was correlated with the poor survival of colorectal cancer patients, and Interferon (IFN‐α/β) stimulation significantly enhanced NK2R gene expression level of colon cancer cells in a Janus kinas 1/2 (JAK 1/2)‐dependent manner. NKA stimulation augmented viability/proliferation and phosphorylation of Extracellular‐signal‐regulated kinase 1/2 (ERK1/2) levels of IFN‐α/β‐treated colon cancer cells and NK2R blockade by using a selective antagonist reduced the proliferation in vitro. Administration of an NK2R antagonist alone or combined with polyinosinic‐polycytidylic acid, a synthetic analog of double‐stranded RNA, to CT26‐bearing mice significantly suppressed tumorigenesis. NK2R‐overexpressing CT26 cells showed enhanced tumorigenesis and metastatic colonization in both lung and liver after the inoculation into mice. These findings indicate that IFN‐α/β‐mediated NK2R expression is related to the malignancy of colon cancer cells, suggesting that NK2R blockade may be a promising strategy for colon cancers.     

颈椎“骨错缝筋出槽”临床评价方法

结合临床研究结果 ,从关节功能评估和关节结构评估两个方面阐述＂骨错缝筋出槽＂临床特征的评价方法。关节功能评估包括动态触诊评估和颈椎自主活动度检测,关节结构评估包括静态触诊评估和影像学评估。     

垂体腺瘤nm23-H_1基因表达的临床意义

目的明确nm23-H_1基因在垂体腺瘤中的表达及其临床意义。方法采用免疫组织化学染色（SP）法对62例垂体腺瘤nm23-H_1基因表达进行回顾性研究。结果侵袭型垂体腺瘤nm-H_1基因表达水平显著低于非侵袭型（P＜0.01）;Ⅲ、Ⅳ级垂体腺癌nm23-H_1基因表达水平显著低于Ⅰ、Ⅱ级（P＜0.05）;nm23-H_1基因表达水平下降与垂体腺瘤患者的性别、年龄及肿瘤分泌功能无显著相关性（P＞0.05）。结论nm23-H_1基因可能在会作腺瘤的侵袭过程中起重要作用,其表达改变是反映垂体腺癌侵袭性的敏感指标。     

Efficacy on rabbits with arrhythmia:needling acupoint of Neiguan(PC6) at shallow or deep depth,and retaining needles for 10,20,or 30 min

OBJECTIVE:To compare the effects of Neiguan(PC6) acupuncture at different depths and reten-tion time on arrhythmia duration,myocardial tissue morphology,mRNA ex...