全文获取类型
收费全文 | 81881篇 |
免费 | 7329篇 |
国内免费 | 5501篇 |
专业分类
耳鼻咽喉 | 845篇 |
儿科学 | 884篇 |
妇产科学 | 1224篇 |
基础医学 | 10581篇 |
口腔科学 | 1403篇 |
临床医学 | 10923篇 |
内科学 | 12324篇 |
皮肤病学 | 1026篇 |
神经病学 | 4502篇 |
特种医学 | 3083篇 |
外国民族医学 | 53篇 |
外科学 | 8520篇 |
综合类 | 10783篇 |
现状与发展 | 21篇 |
一般理论 | 22篇 |
预防医学 | 5124篇 |
眼科学 | 2984篇 |
药学 | 8469篇 |
67篇 | |
中国医学 | 4163篇 |
肿瘤学 | 7710篇 |
出版年
2024年 | 259篇 |
2023年 | 1221篇 |
2022年 | 3185篇 |
2021年 | 4106篇 |
2020年 | 3010篇 |
2019年 | 2888篇 |
2018年 | 3071篇 |
2017年 | 2543篇 |
2016年 | 2641篇 |
2015年 | 3799篇 |
2014年 | 4717篇 |
2013年 | 4163篇 |
2012年 | 6174篇 |
2011年 | 6610篇 |
2010年 | 4080篇 |
2009年 | 3084篇 |
2008年 | 4283篇 |
2007年 | 4165篇 |
2006年 | 4251篇 |
2005年 | 4083篇 |
2004年 | 2772篇 |
2003年 | 2536篇 |
2002年 | 2083篇 |
2001年 | 1788篇 |
2000年 | 1774篇 |
1999年 | 2054篇 |
1998年 | 1296篇 |
1997年 | 1265篇 |
1996年 | 956篇 |
1995年 | 904篇 |
1994年 | 767篇 |
1993年 | 482篇 |
1992年 | 596篇 |
1991年 | 497篇 |
1990年 | 472篇 |
1989年 | 412篇 |
1988年 | 371篇 |
1987年 | 300篇 |
1986年 | 248篇 |
1985年 | 210篇 |
1984年 | 130篇 |
1983年 | 86篇 |
1982年 | 42篇 |
1981年 | 51篇 |
1980年 | 29篇 |
1979年 | 63篇 |
1978年 | 22篇 |
1974年 | 27篇 |
1973年 | 18篇 |
1968年 | 16篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
91.
日本血吸虫感染小鼠脾细胞IL—2及IFN—γ动态观察 总被引:4,自引:0,他引:4
本文观察8wk龄C57BL/6小鼠感染日本血吸虫尾蚴后不同时期脾细胞经SEA或ConA刺激,体外诱生的IL-2和IFN-γ活性变化。结果表明两种细胞因子活性均在感染后第4-6wk开始上升,第6—8wk达高峰,第12—14wk恢复至感染前水平。IFN-γ高峰时间略先于IL-2。非特异性刺激原诱生组和特异性刺激原诱生组各阶段细胞因子活性动态基本一致,前组活性高于后组。提示IFN-γ与IL-2活性与血吸虫卵肉芽肿的诱导、成熟与维持有密切关系。 相似文献
92.
系统地研究了肟类药物对塔崩抑制的大鼠脑AChE的体外重活化作用,并与梭曼、沙林和VX进行了比较。结果表明,沙林和VX抑制的AChE较易被药物重活化,而塔崩和梭曼抑制的AChE则较难。37℃、pH7.2条件下,塔崩抑制的大鼠脑AChE可浓度依赖性地被TMB_r和LuH_6重活化,2-PAM在高浓度下也有一定作用,但HI-6在所用3个浓度下均无重活化。通过降低抑制温度成功地建立了未老化的梭曼膦酰化AChE模型。药物试验表明,未老化的梭曼膦酰化大鼠脑AChE可被高浓度(1mmol/L)HI-6重活化,而不被2-PAM、TMB_4及LuH_6重活化。提示药物自身内在活性在重活化作用中的重要性。TMB_4和LuH_6对塔崩磷酸化AChE有较强重活化,而对未老化梭曼膦酸化AChE无重活化,HI-6则相反,对未老化梭曼膦酰化AChE重活化效果好,而对塔崩磷酰化AChE无重活化作用。塔崩和梭曼膦酰化AChE在未老化以前对药物的响应就有所不同,毒剂残基的空间效应可能起重要作用。 相似文献
93.
94.
X F Sun H Zhang X C Wu Y M Han G Q Hou M S Xian 《Acta oncologica (Stockholm, Sweden)》1992,31(1):37-40
The microcirculatory architecture of normal tissue, transitional mucosa and adenocarcinoma of the human colon was investigated with microvascular corrosion casting (MVCC) combined with scanning electron microscopy (SEM). The study showed that the capillaries within the normal mucosa were arranged in a regular hexagonal pattern around the mucosal glands and that the microvessels of transitional mucosa mostly had lost the typical hexagonal pattern and become slightly wider in diameter. The microvessels in the tumor periphery were increased in number and disorganized, and presented large variation in morphology with claw-like formations, widened sinuses, diverticula and appendixoid patterns. Microvessels were lacking in the central areas of tumors. These morphological alterations may serve as additional indicators of tumor development. 相似文献
95.
(+)-CC-1065 is a potent antitumor antibiotic produced by Streptomyces zelensis. Previous studies have shown that the potent cytotoxic and antitumor activities of (+)-CC-1065 are due to the ability of this compound to covalently modify DNA. (+)-CC-1065 reacts with duplex DNA to form a (N3-adenine)-DNA adduct which lies in the minor groove of DNA overlapping with a five base-pair region. As a consequence of covalent modification with (+)-CC-1065, the helix bends into the minor groove and also undergoes winding and stiffening. In the studies described here, we have constructed templates for helicase-catalyzed unwinding of DNA that contain site-directed (+)-CC-1065 and analogue DNA adducts. Using these templates we have shown that (+)-CC-1065 and select synthetic analogues, which have different levels of cytotoxicity, all produce a significant inhibition of unwinding of a 3'-tailed oligomer duplex by helicase II when the displaced strand is covalently modified. However, the extent of helicase II inhibition is much more significant for (+)-CC-1065 and an analogue which also produced DNA winding when the winding effects are transmitted in the opposite direction to the helicase unwinding activity. This observed pattern of inhibition of helicase-catalyzed unwinding of drug-modified templates was the same for a 3'-T-tail, for different duplex region sequences, and with the Escherichia coli rep protein. Unexpectedly, the gel mobility of the displaced drug-modified single strand was dependent on the species of drug attached to the DNA. Last, strand displacement by helicase II coupled to primer extension by E. coli DNA polymerase I showed the same pattern of inhibition when the lagging strand was covalently modified. In addition, the presence of helicase II on single-stranded regions of templates caused the premature termination of primer extension by DNA polymerase. These results are discussed from the perspective that (+)-CC-1065 and its analogues have different effects on DNA structure, and these resulting structural changes in DNA molecules are related to the different in vivo biological consequences caused by these drug molecules. 相似文献
96.
中药黄精中的新生物碱 总被引:10,自引:0,他引:10
中药黄精中的新生物碱孙隆儒1)王素贤李铣(沈阳药科大学天然药物研究室,沈阳110015)黄精为百合科黄精属植物黄精(PolygonatumsibiricumRedoute)的根茎,具有补气养阴、FigStructureofthecompound健脾、... 相似文献
97.
创收工资—医院按劳分配的探索 总被引:1,自引:1,他引:0
遵循“各尽所能,按劳分配”的社会主义分配原则,我院对职工分配进行了积极的探索。从80年代初起,在院内实行定量、定质的超额劳务提成。但随着医院改革不断深化,医院总收入增加,院内、外独立核算,限期还本息项目增多,原超额劳务提成办法不能适应医院发展。因此,在原有基本工资、奖励工资基础上,增设了创收工资,医院对创收工资从质量、数量、文明建设、医德医风建设等方面进行宏观调控。创收工资使科主任责、权、利落到实 相似文献
98.
白细胞介素—2新的功能位点及其中枢镇痛作用 总被引:2,自引:0,他引:2
白细胞介素-2(IL-2)不仅是重要的免疫调节因子,而且还具有重要的中枢调节作用。本实验以钾离子透入引起大鼠甩尾反应为指标,发现侧脑室注射IL—2能显著提高动物痛阈,并能被纳洛酮所阻断,表示IL-2的中枢镇痛作用可能与阿片受体有关。利用基因定位突变技术获得的无免疫活性IL-2实查体仍具有中枢镇痛作用,表明IL—2分子上发挥镇痛和免疫调节作用的功能位点是相互独立的。纳洛酮能够阻断IL—2的中枢镇痛作用,而不能影响IL—2增殖CTLL-2细胞的作用,提示IL-2发挥镇痛和免疫调节作用可能通过不同的受体途径。IL-2分子中第45位Tyr残基突变为Val后,虽仍保留了免疫活性,但丧失了镇痛功能,表示45位Tyr残基是IL—2发挥中枢镇痛功能的关键残基之一。我们推测IL—2的镇痛功能位点可能在IL—2分子中第45位Tyr残基附近区域。 相似文献
99.
Tissue and cell homogenates were prepared for PG and LDH study from 20 samples of histologically proven gastric cancer (GC), 6 samples of gastric cancer xenografts (THPGC-1) of different passages (GCXG) and cultured cells of 3 different gastric cancer cell lines (GCCL). Normal gastric mucosa (NGM) was also obtained from the resected stomach far distant from the primary tumor and histologically tumor free. The results indicated that the expression of PG isoenzymes was low or absent and the PG activities were significantly decreased in GC, GCXG and GCCL as compared to NGM. The activity of LDH was also significantly increased in GC, GCXG and GCCL. In addition, there was a change in isoenzyme pattern in GC and GCXG in which isoenzyme type M was observed whereas isoenzyme type H was preponderent in NGM. The results show that the human gastric cancer xenograft, THPGC-1, has biological properties very similar to those of the primary tumor suggesting that THPGC-1 is a reliable model for the study of the molecular biology of human gastric cancer. 相似文献
100.