46例Turner综合征患者临床特征及染色体分析

目的Turner综合征患者身材矮小伴不同程度的性腺发育不全,探讨Turner综合征不同核型的遗传学特征、临床特点及其所占比例。方法高龄或高危孕妇产前诊断及成人外周血染色体核型分析。结果产前诊断发现Turner综合征9例,成人外周血检测发现Turner综合征37例,46例患者中45,X25例(54.35%);45,X/46,XX嵌合型4例(8.70%);45,X/46,X,i(X)嵌合型3例(6.52%);46,X,i(X)2例(4.35%);46,XX,inv(X)2例(4.35%);46,XY7例(15.22%);45,X/46,XX/47,XXX嵌合型,46,XX,t(X;3)和45,X/46,XX,del(X)各1例,分别占2.17%。结论Turner综合征包括X染色体数目异常和结构畸变等多种核型,均可不同程度导致女性不孕、智力低下及其他器官功能异常,应提倡优生优育,做好产前诊断。     

应用微波与激光治疗子宫颈糜烂的对比观察

将３００例慢性宫颈炎妇女随机分为微波组和激光组，分别采用微波组织凝固和ＣＯ＿２激光汽化进行治疗。结果：微波治疗宫颈糜烂一次治愈率９２．２０％（１４２／１５４），ＣＯ＿２激光一次治愈率７９．４５％（１１６／１４６）。两组治疗结果比较，差异有非常显著意义（Ｐ＜０．００５）。两组治疗后的不良反应大致相同，术中出血的发生率，微波组３．２５％（５／１５４），明显低于激光组２１．９２％（３２／１４６），差异有非常显著意义（Ｐ＜０．００５）。研究结果表明：微波组织凝固技术治疗宫颈糜烂一次治愈率高，术中止血效果好，操作简单，有实用价值。     

Do gender and birth height of infant affect calorie of human milk? An association study between human milk macronutrient and various birth factors

Objective: The purpose of this study is to analyze the macronutrient of human milk (HM) and to find out the various maternal–infantile factors that can affect HM composition.

Methods: 478 HM samples were collected from healthy and exclusively breast-feeding mothers who delivered healthy term neonates within 3 months. Macronutrient of the samples was analyzed and the birth data were collected.

Results: In multivariate logistic regression analysis, various maternal–infantile factors were found to be associated with HM composition changes; higher fat: cesarean section (OR?=?2.47, p?p?=?0.004); higher protein: postpartum age (OR?=?0.89, p?p?=?0.005) and female infant (OR?=?0.56, p?=?0.012); higher calorie: postpartum age (OR?=?0.95, p?=?0.003), female infant (OR?=?0.33, p?=?0.017), and birth height (OR?=?0.74, p?p?=?0.029), birth height (OR?=?0.73, p?=?0.001), and postpartum age (OR?=?0.95, p?=?0.005) were found as independent risk factors for higher HM calorie.

Conclusion: Various maternal–infantile factors were found to affect HM composition. Interestingly, delivery mode, gender of infant, and birth height were associated with changes in HM macronutrient as well as postpartum age.     

子宫内膜异位症患者腹腔液中IL-6、IL-8、IL-10含量升高及其临床意义

目的：检测子宫内膜异位症（endometriosis,EMS）患者腹腔液中IL-6、IL-8、IL-10含量及其临床意义。方法：剖腹探查手术中采集EMS组及对照组的腹腔液,用酶联免疫吸附法（ELISA）测细胞因子水平。EMS组中,13份测IL-6,16份测IL-8,22份测IL-10,共51份,对照组检测57份。结果：EMS组和对照组IL-6的含量分别为155．88±114．92ng／L和28．74±25．88ng／L（P＜0．01）。IL-8的含量分别为630．97±404．72ng／L和63．05±62．3ng／L（P＜0．01）。IL-10也明显高于对照组［（38．88±38．75ng／L比10．45±4．33ng／L（P＜0．01）〕。结论：EMS患者腹腔液中IL-6、IL-8及IL-10均升高,提示EMS患者的巨噬细胞活性增强,细胞因子含量增加,可干扰患者免疫调节功能。     

Expression of cyclooxygenase-2 in association with clinicopathological prognostic factors and molecular markers in epithelial ovarian cancer

OBJECTIVES: This study examines whether expression of COX-2 is associated with clinicopathological features and other molecular markers of ovarian cancer. METHODS: Sixty-four paraffin-embedded tissue specimens were obtained from patients with ovarian cancer who received cytoreductive surgery and combination chemotherapy. Tissue specimens were subjected to immunohistochemical analysis using antibodies to COX-2, p53, and VEGF. RESULTS: Increased COX-2 expression significantly correlated with histologic type (mucinous 5.6% vs. non-mucinous 65.2%, P<0.001). COX-2 expression was also significantly associated with stage, tumor grade, residual disease status, and presence of ascites. COX-2 expression correlated positively with expression of p53 (P=0.006) and VEGF (P=0.025). Although survival was lower in patients with high COX-2 expression than in those without high COX-2 expression (P<0.001), only tumor grade and stage were independent prognostic indicators. In patients with non-mucinous cancer, COX-2 expression correlated with stage (P<0.001) and presence of ascites (P=0.033). CONCLUSIONS: Our results suggest that expression of COX-2 in ovarian cancers is specific to histologic type of tumor and is associated with poor clinicopathologic prognostic factors. Expression of COX-2 also correlates well with expression of p53 and VEGF.     

MMP-2、TIMP-2、Ki-67在库肯勃氏瘤中的表达及意义

目的 :探讨MMP 2、TIMP 2、Ki 67表达与库肯勃氏瘤病理生物学行为的关系。方法 :采用免疫组化SP法对 32例库肯勃氏瘤组织和 10例正常卵巢组织进行MMP 2、TIMP 2和Ki 67表达的检测。结果 :MMP 2、TIMP 2、Ki 67在库肯勃氏瘤组织中的表达量显著高于正常卵巢组织 (P <0 .0 1)。有合并转移的库肯勃氏瘤中MMP 2、Ki 67高于无合并转移者 (P <0 .0 5 ) ,TIMP 2低于无合并转移者 (P <0 .0 5 )。MMP 2、Ki 67与术后生存时间呈负相关 (P <0 .0 1) ,TIMP 2与术后生存时间呈正相关 (P <0 .0 1)。结论 :MMP 2、TIMP 2、Ki 67在库肯勃氏瘤发生、发展中起重要作用 ,可作为判断卵巢转移癌恶性程度、临床分期和评估预后的临床参考指标     

Risk of infections of biological and targeted drugs in patients with spondyloarthritis: meta-analysis of randomized clinical trials

Background: Concerns exist regarding the risk of infections in patients with spondyloarthritis (SpA) treated with biologics. We assessed the risk of infections of biological and targeted drugs in patients with SpA by performing a meta-analysis based on randomized controlled trials (RCTs).Methods: A systematic literature search was conducted in PubMed, Embase, Web of Science, the Cochrane Library, and China Biology Medicine Disc for RCTs evaluating the risk of infections of biological therapy in ...     

螺旋霉素产生菌螺旋霉素链霉菌遗传育种

本文是利用抗生素抗性与产量之间的相关性来进行螺旋霉素产生菌螺旋霉素链霉菌的遗传育种,旨在获得高效价菌株,从而提高螺旋霉素的生产水平。首先用正交试验法确定出发菌株最佳发酵培养基,并在此基础上通过紫外线诱变,和在螺旋霉素浓度梯度平板上筛选螺旋霉素抗性菌株。按上述方法理性化筛选得到的菌株多为正突变株。从本实验得到一株螺旋霉素抗性菌株SPM~r-248,其抗性较出发菌株提高300％,生产能力较出发菌株提高81.3％。菌株SPM~r-248的高效价生产性能遗传特性稳定。在7m~3罐做发酵放大,与出发菌株相比,发酵效价提高31.4％,发酵指数提高18.8％,具有一定的学术意义和经济价值。