人乳头瘤病毒感染的局部免疫反应

本文应用ABC法对30例尖锐湿疣(CA)和30例宫颈癌(CCU)进行原位观察,分析对比两者浸润单一核细胞(MNC)的亚群组成、分布及活化状态。结果提示,两者局部免疫均受抑制,而以宫颈癌为甚。依此本文对不同类型HPV相关疾病的局部免疫反应状态及宿主对不同型别HPV感染的免疫反应进行探讨。     

用标记分析法检测我国海南省登革2型病毒抗原性变化

我们试用标记分析法,用3种单克隆抗体(黄病毒科特异性、登革病毒属特异性和登革病毒2型型特异性单克隆抗体)研究了我国海南省1985～1987年3年内流行的登革2型病毒8个流行株的抗原性变化,并与标准株新几内亚B株进行了比较。发现6株与标准株类似,2株显示出明显的差异。标记分析法为病毒抗原分析提供了一个简便、快速的方法,可用来监测一个地区病毒株群的变化及新株的传入。     

CD3ζ链基因在脐带血T细胞及其CD4+和CD8+T细胞亚群中的表达特点

目的 了解脐带血T细胞及其CD4 和CD8 T细胞亚群的信号传导分子CD3ζ基因的表达特点.方法 采用SYBR Green Ⅰ荧光定量PCR和相对定量分析法检测60例脐带血单个核细胞和12例纯化脐带血CD4 及CD8 T细胞的CD3ζ基因的表达情况,以β2微球蛋白基因(β2M)作为内参,采用相对定量公式:2-△Ct×100%,计算CD3ζ基因相对mRNA表达量,60例健康成人作为对照.并根据荧光定量PCR熔解曲线特点和序列分析了解CD3ζ基因突变情况.结果 全部脐带血和健康成人外周血单个核细胞均表达CD3ζ基因,不同个体CD3ζ基因表达量有所差异,脐带血T细胞CD3ζ基因相对mRNA表达量为6.7%±5.56%,CD4 和CD8 T细胞的CD3ζ基因相对mRNA表达量分别为6.74%±2.0%和6.88%±1.76%,三者CD3ζ基因表达量均明显高于健康成人(P=0.000,P=0.034,P=0.000).序列分析结果显示尚未发现国外文献所报道的ζ链剪接异构体.结论 本研究率先报道了脐带血T细胞及其CD4 和CD8 T细胞亚群的CD3ζ基因mRNA的表达水平,为进一步了解脐带血T细胞免疫学特点提供新的基础资料.     

Afferent innervation of the utricular macula in pigeons

Biotinylated dextran amine (BDA) was used to retrogradely label afferents innervating the utricular macula in adult pigeons. The pigeon utriclar macula consists of a large rectangular-shaped neuroepithelium with a dorsally curved anterior edge and an extended medioposterior tail. The macula could be demarcated into several regions based on cytoarchitectural differences. The striola occupied 30% of the macula and contained a large density of type I hair cells with fewer type II hair cells. Medial and lateral extrastriola zones were located outside the striola and contained only type II hair cells. A six- to eight-cell-wide band of type II hair cells existed near the center of the striola. The reversal line marked by the morphological polarization of hair cells coursed throughout the epithelium, near the peripheral margin, and through the center of the type II band. Calyx afferents innervated type I hair cells with calyceal terminals that contained between 2 and 15 receptor cells. Calyx afferents were located only in the striola region, exclusive of the type II band, had small total fiber innervation areas and low innervation densities. Dimorph afferents innervated both type I and type II hair cells with calyceal and bouton terminals and were primarily located in the striola region. Dimorph afferents had smaller calyceal terminals with few type I hair cells, extended fiber branches with bouton terminals and larger innervation areas. Bouton afferents innervated only type II hair cells in the extrastriola and type II band regions. Bouton afferents innervating the type II band had smaller terminal fields with fewer bouton terminals and smaller innervation areas than fibers located in the extrastriolar zones. Bouton afferents had the most bouton terminals on the longest fibers, the largest innervation areas with the highest innervation densities of all afferents. Among all afferents, smaller terminal innervation fields were observed in the striola and large fields were located in the extrastriola. The cellular organization and innervation patterns of the utricular maculae in birds appear to represent an organ in adaptive evolution, different from that observed for amphibians or mammals.     

蒙药毛勒日—达布斯—4汤中微量元素及氨基酸含量的测定

目的：测定蒙药毛勒日-达布斯-4汤中微量元素和氨基酸的含量。方法：用原子吸收分光光度计,测定蒙药毛勒日-达布斯-4汤中几种重要的微量元素含量;用氨基酸自动分析仪测定氨基酸的含量。结果与结论：蒙药毛勒日-达布斯-4汤中含有较高的Mn、Fe、Zn和Mg;含有17种氨基酸,包括7种人体必需氨基酸,氨基酸总量为4.615%（mg/g）。     

冠状静脉逆灌注对犬缺血心肌的保护作用

【目的】探索一种简便的冠状静脉逆灌注方法,了解其有效性和安全性。【方法】健康成年犬13条,随机分为逆灌注组（n=7）和对照组（n=6）。开胸结扎左冠状动脉前降支（LADA）,逆灌注导管经右颈外静脉送入冠状静脉窦并进一步插至心大静脉（GCV）远端,动脉血由左颈总动脉引出,以自身动脉血压为动力进行持续逆灌注。逆灌注组于LADA结扎后5min开始持续逆灌注,共6h;对照组同样进行插管但不灌注。观察血流动力学、心电图、血清生化及形态学改变。【结果】逆灌注组血流动力学较稳定（P＜0.05～0.01）。LADA结扎后两组缺血中心区心外膜心电图ST段均明显抬高,30min后逐渐回降,逆灌注组回降速度较快（P＜0.01）。与逆灌注组相比,对照组血清肌酸激酶-MB（CK-MB）明显升高,一氧化氮（NO）水平明显下降（P＜0.05～0.01）。对照组和逆灌注组缺血心肌发生梗死的面积分别占（76.2±4.7）%和（38.8±7.3）%（P＜0.01）。光镜下,逆灌注组前室间静脉（LADV）及其小属支内膜结构完整,无明显损伤;两组均未见到出血性梗死。【结论】采用简便的冠状静脉逆灌注方法可明显减轻心肌的缺血性损伤,减小心肌梗死面积,稳定血流动力学,且对冠状静脉系统及心肌组织无明显损伤。     

HPV在小儿喉乳头状瘤中的表达及临床意义

目的：探讨HPV与小儿喉乳状瘤发病及发展的相关性。方法：采用免疫组化方法检测46例小儿喉乳头状瘤96次手术的肿瘤组织及其HPV表达,另取8例正常小儿及成人的喉黏膜,10例成人喉乳头状瘤作对照,结果：小和和成人的正常喉黏膜无HPV表达,成人喉乳头状瘤2例有HPV表达,46例96人次小儿喉乳头状瘤,其中33例61人次肿瘤组织中有HPV表达,且与肿瘤的复发频率呈正相关。结论HPV在小儿喉乳头状瘤的发生发展过程中起重要作用。     

Efficacy of testosterone replacement therapy for treating metabolic disturbances in late-onset hypogonadism: a systematic review and meta-analysis

International Urology and Nephrology - Late onset hypogonadism (LOH) is an age-dependent reduction of testosterone associated with alterations of metabolic profile, including glucose control,...     

Supratentorial pediatric cortical ependymomas: a comprehensive retrospective study

Neurosurgical Review - Pediatric cortical ependymomas (CEs) are rare; the clinical features and optimal treatment remain ill-defined. We aimed to clarify the clinical characteristics and outcome of...     

Clinicopathological characteristics,prognosis, and chemosensitivity in patients with metastatic upper tract urothelial carcinoma

PurposeTo investigate the clinical characteristics, chemosensitivity, and outcome of metastatic upper tract urothelial carcinoma (UTUC).Patients and MethodsRecords of patients with metastatic UTUC since January 2005 were retrieved from a database that included clinical and survival data. Statistical analyses including survival and multivariate analyses of factors were respectively performed by the Kaplan-Meier method and Cox proportional hazard model.ResultsA total of 250 consecutive UTUC cases were evaluated. There were 56 patients (22.4%) with initially diagnosed stage IV disease. The most common metastatic sites were lung (39.6%), distant lymph nodes (39.2%), bone (19.6%), liver (18.0%), and adrenal gland (7.2%), respectively, and the local recurrence rate was 10.4%. Two hundred thirteen patients received first-line chemotherapy. The overall response rate was only 28.7% and the median progression-free survival time was only 5.0 months. The overall survival time of the cohort was 18.0 months. Multivariate analyses showed that initially diagnosed stage IV disease, number of metastatic organs ≥3, no response to chemotherapy and cycles of chemotherapy ≤2 were adverse prognosticators for overall survival.ConclusionUTUC presented to be more prone to metastasize than locally recur and thought to have low chemosensitivity. Stage IV disease at initial diagnosis, number of metastatic organs, response and cycles of chemotherapy were independent prognosticators for metastatic UTUC.