五仁液有效成分分析

应用GC/MS联用技术研究了五仁液有效成分及其相对含量,发现五仁液中含有丰富的酚类、苯甲酸类、脂肪酸等抗微生物的有效成分。该结果证实了五仁液消毒、杀菌和治疗皮肤病的有效性。     

Regulation of PPAR-γ activity in lipid-laden hepatocytes affects macrophage polarization and inflammation in nonalcoholic fatty liver disease

BACKGROUNDLipid metabolism disorder and inflammatory-immune activation are vital triggers in the pathogenesis of nonalcoholic fatty liver disease (NAFLD). Various studies have shown that PPAR-γ exerts potent anti-inflammatory and immunomodulatory properties. However, little is known about the regulation of PPAR-γ activity in modulating cell crosstalk in NAFLD.AIMTo investigate whether the regulation of PPAR-γ activity in lipid-laden hepatocytes affects macrophage polarization and inflammation.METHODSPrimary hepatocytes were isolated from wild-type C57BL6/J mice or hepatocyte-specific PPAR-γ knockout mice and incubated with free fatty acids (FFAs). Macrophages were incubated with conditioned medium (CM) from lipid-laden hepatocytes with or without a PPAR-γ agonist. Wild-type C57BL/6J mice were fed a high-fat (HF) diet and administered rosiglitazone.RESULTSPrimary hepatocytes exhibited significant lipid deposition and increased ROS production after incubation with FFAs. CM from lipid-laden hepatocytes promoted macrophage polarization to the M1 type and activation of the TLR4/NF-κB pathway. A PPAR-γ agonist ameliorated oxidative stress and NLRP3 inflammasome activation in lipid-laden hepatocytes and subsequently prevented M1 macrophage polarization. Hepatocyte-specific PPAR-γ deficiency aggravated oxidative stress and NLRP3 inflammasome activation in lipid-laden hepatocytes, which further promoted M1 macrophage polarization. Rosiglitazone administration improved oxidative stress and NLRP3 inflammasome activation in HF diet-induced NAFLD mice in vivo.CONCLUSIONUpregulation of PPAR-γ activity in hepatocytes alleviated NAFLD by modulating the crosstalk between hepatocytes and macrophages via the reactive oxygen species-NLRP3-IL-1β pathway.     

益气化瘀补肾法对退变颈椎间盘Ⅱ型胶原mRNA表达的影响

目的益气化瘀补肾法对退变颈椎间盘Ⅱ型胶原mRNA表达的影响。方法健康成年新西兰大白兔42只,随机平均分为益气化瘀补肾组、益气化瘀组、补肾组、消炎组、模型对照组、正常对照组。建立颈椎病椎间盘退变模型,原位杂交后病理图像分析。结果正常对照组髓核组织中Ⅱ型胶原mRNA表达的量显著高于模型对照组和消炎痛治疗组（P<0.01）,与益气化瘀组、补肾组相比较也有显著差异(P<0.05);益气化瘀补肾法与益气化瘀法、单纯补肾法比较有较显著差异(P<0.05),与消炎痛组、模型组比较有非常显著差异(P<0.01);益气化瘀法、补肾法对髓核组织中Ⅱ型胶原mRNA的表达有一定的增强作用。结论颈椎病家兔椎间盘的Ⅱ型胶原mRNA表达比正常组显著降低,益气化瘀补肾法可以上调Ⅱ型胶原mRNA基因的表达,使之维持在正常范围,说明益气化瘀补肾法对延缓椎间盘的退变有积极作用。     

雄黄诱导肿瘤细胞凋亡的实验研究

目的 观察中药雄黄诱导肿瘤细胞凋亡的作用。方法 以细胞凋亡和免疫功能为主要观测指标,观察中药雄黄对荷瘤小鼠的抗肿瘤作用。结果 实验表明,雄黄可诱导荷瘤（Ｌ－１２１０和Ｈ２２）小鼠的肿瘤组织凋亡,抑制荷瘤小鼠肿瘤的生长,并可延长小鼠存活时间。结论 雄黄可通过诱导肿瘤细胞凋亡,抑制细胞ＤＮＡ的合成,增强机体的细胞免疫功能等多种因素发挥抗肿瘤作用,显示出广阔的应用前景。     

妥布霉素玻璃酸钠滴眼液在超声乳化白内障摘出术后的应用

目的评价妥布霉素玻璃酸钠滴眼液在超声乳化白内障摘出术后应用的临床有效性和安全性。方法随机、双盲筛选出白内障患者121例（121眼）施行超声乳化白内障摘出联合人工晶状体植入术，分为试验组和对照组，试验组术后滴用妥布霉素玻璃酸钠滴眼液，对照组术后滴用妥布霉素地塞米松滴眼液，分别于术后第1d、用药后第3、7、14d观察眼部症状和体征，并进行统计学分析。结果两组术后泪膜破裂时间、结膜充血、睫状充血、房水闪辉等比较，差异有统计学意义（P〈0．05）；角膜内皮细胞计数、视力、眼压等比较差异无统计学意义（P〉0．05）。结论对部分手术创伤小，反应轻的超声乳化白内障摘出术后的患者可以使用妥布霉素玻璃酸钠滴眼液。     

Detection of abnormal visual cortex in children with amblyopia by voxel-based morphometry

PURPOSE: To detect the abnormalities of gray matter in children with amblyopia by voxel-based morphometry (VBM). DESIGN: Prospective, nonrandomized clinical trial. METHODS: Thirteen children with amblyopia and 14 normally sighted children underwent magnetic resonance (MR) examination. The two groups were age-matched with a mean age of 5.8 years. In the amblyopia group, five children had strabismus amblyopia, and eight had anisometropic amblyopia. We analyzed the original 3-dimensional T1 brain images using the VBM module within the widely used analysis software package SPM2 (Welcome Department of Cognitive Neurology, London, United Kingdom). After normalization, segmentation, and smoothing of the images, comparison between amblyopic and control groups was derived for the gray matter of the entire brain using parametric statistics. RESULTS: The results of VBM analysis indicated that the amblyopic group had decreased gray matter density in the middle frontal gyrus, parahippocampal gyrus, fusiform gyrus, inferior temporal gyrus of the left hemisphere, and the bilateral calcarine cortices. The radii of these regions ranged from 12 to 36 voxels. These abnormalities were consistent with morphologic changes in brain regions related to visual function. CONCLUSIONS: Using MR and VBM analysis, we detected morphologic changes in the visual cortex of children with amblyopia, which may indicate developmental abnormalities of visual cortex during the critical growth period.     

Surgical and Oncologic Outcomes of Radical Abdominal Trachelectomy Versus Hysterectomy for Stage IA2-IB1 Cervical Cancer

Study Objective: To compare the surgical and oncologic outcomes between abdominal radical trachelectomy (ART) and radical hysterectomy (RH) for stage IA2-IB1 cervical cancer.Design: A retrospective cohort study (Canadian Task Force classification II-2).Setting: Shandong Cancer Hospital, Shandong, China.Patients: Three hundred twenty-nine patients with IA2-IB1 cervical cancer.Interventions: All patients underwent ART or RH.Measurements and Main Results: All patients were divided into ART (n?=?143) and RH (n?=?186) groups according to the surgical approach. Additionally, oncologic and fertility outcomes were compared for different tumor pathologies and sizes in ART patients. The ART group had similar case characteristics as the RH group, except that the ART group had a longer surgical time. During a similar follow-up period, there were 4 (2.9%) recurrences and 3 (2.2%) patients who died from recurrence in the ART group compared with 8 (4.6%) recurrences and 4 (2.3%) patients who died from recurrence in the RH group (p?=?.444 and p?=?.999, respectively). In the ART group, squamous cell carcinoma (SCC) patients had a 5-year overall survival and pregnancy rate similar to those of non-SCC patients (98.1% vs 96.8%, p?=?.999; 33.3% vs 26.7%, p?=?.873), and patients with tumors ≤2 cm and 2 to 4 cm experienced a similar 5-year overall survival rate (97.0% vs 98.6%, p?=?.999), except patients with tumors ≤2 cm had a higher pregnancy rate (45.2% vs 17.2%, p?=?.020).Conclusion: ART seems to have similar surgical and oncologic outcomes to RH, except ART has a longer surgical time. Both non-SCC patients and stage IA2-IB1 patients with 2- to 4-cm tumors can undergo ART safely. Patients with tumors ≤2 cm have a higher pregnancy rate than patients with 2- to 4-cm tumors.     

细胞因子IL-4对小鼠骨髓来源树突状细胞培养的影响

目的:建立从小鼠骨髓中分离、纯化、培养、扩增树突状细胞(DC)前体的方法,研究细胞因子IL-4对小鼠DC体外增殖、分化成熟的影响.方法:将健康小鼠股骨骨髓细胞分离,去除悬浮细胞,将贴壁细胞分为2组,第1组加入重组细胞/巨噬细胞集落刺激因子(GM-CSF)培养;第2组加入GM-CSF和白细胞介素-4(IL-4)联合培养.诱导的DC经相差显微镜观察.结果:分离的DC前体经8～9d的培养,镜下随机取8个视野进行细胞计数,第1组细胞数量是85.38±3.42;第2组细胞数量是119.25±3.96,两组间DC数量有显著差异(t=3.19,P<0.05).且细胞纯度达90%以上,具有典型的树枝状或裙褶状突起.结论:用GM-CSF和IL- 4联合作用更能促进DC的体外扩增及分化.     

SARS病人治愈后随访13个月细胞因子指标的变化

目的探讨严重急性呼吸综合征（SARS）病人远期细胞因子动态变化及规律。方法采用酶联免疫吸附法（ELISA）检测SARS病人痊愈后mo4、mo10及mo13的血清中IL—1β、TNFα,、TGFβ水平。结果111例病人中,IL—1β升高比率在随访的mo4、mo10、mo13分别为0％、48．6％、18．9％;TNFα升高比率在随访的mo4、mo10、mo13分别为26．1％、0．8％、3．6％;TG邱升高比率在随访的mo4、mo10、mo13分别为93．7％、97．3％、100％。结论随访SARS病人康复后血清的IL—1β、TNFα,和TGF-β水平呈波动状态,炎性细胞因子呈递降趋势,而抗炎性因子呈递升趋势,这种趋势发展是一个长期的过程。