Immunoaffinity chromatography for purification of Salbutamol and Clenbuterol followed screening and confirmation by ELISA and GC-MS

Screening and confirmatory methods for the determination of Salbutamol (SAL) and Clenbuterol (CL) in swine urine were established. The polyclonal antibody against SAL was prepared, which was used to develop the indirect competitive enzyme-linked immunosorbent assay (ELISA) with the limit of detection of 0.5 ng/ml, and to prepare the immunoaffinity chromatography column. The immunoaffinity column could extract and purify SAL and CL simultaneously, with a binding capacity of 400 ng for SAL and 416 ng for CL. After purification, the swine urine samples were screened by ELISA for the presence of SAL and/or CL, and the positive samples were further confirmed and quantified by gas chromatography-mass spectrometry (GC-MS). The results showed that 95% of the positive samples were confirmed by GC-MS with various levels of SAL (1.1-4.6 ng/ml) and/or CL (1.9-229.1 ng/ml) residues in the incurred samples, and all the negative samples were confirmed with no SAL and/or CL residues.     

心电心向量同步仪的研制

本文介绍一种以３８６计算机为核心的心电心向量同步仪。阐述了仪器的工作原理、硬件组成、软件设计。     

111例急性T淋巴细胞白血病免疫表型分析

选用一组抗人白细胞表面分化抗原单克隆抗体和基因探针,依靠间接免疫荧光技术、Ap-AAP桥联酶标免疫组化染色和分子杂交手段.对111例急性T淋巴细胞白血病(T-ALL)进行了免疫分型研究。本研究所观察到的T-ALL细胞表型绝大多数反映着正常胸腺细胞分化发育阶段或某些少见类型细胞的表型特征,亦有少数病例则无正常淋巴细胞相对应的表型,表明T-ALL细胞的高度异质性。对T-ALL表型的研究为今后深入探讨胸腺内淋巴细胞的分化成熟以及T-ALL发生学提供了良好的研究模式。     

Functional expression of 4-1BB (CD137) in the inflammatory tissue in Crohn's disease

4-1BB ligand (L) expressed on antigen presenting cells (APC) interacts with 4-1BB, expressed on activated T cells and this interaction costimulates T cells to secrete cytokines and to proliferate. We investigated whether 4-1BB/4-1BBL interactions might be involved in the pathogenesis of Crohn's disease (CD). In immunohistochemistry, we found 4-1BB expression on lamina propria (LP) cells in inflamed and to a lesser extend in non-inflamed gut tissue from CD patients. mRNA levels for 4-1BB were also elevated in intestinal CD tissue. In contrast, only few 4-1BB-expressing cells were found in inflamed tissue from ulcerative colitis (UC) patients and almost no positive cells were found in control intestinal tissue. 4-1BB expression was better sustained on in vitro activated lamina propria T cells from CD patients compared to controls. Finally, agonistic anti-4-1BB antibody enhanced interferon-gamma (IFN-gamma) production and proliferation of lamina propria T cells from CD patients. Taken together, our data suggest that 4-1BB/4-1BBL interactions contribute to the persistence of gut inflammation in CD.     

Isolation of Nanjing local strains of HHV-7 and their biological and immunological characteristics

To investigate the biological and immunological characteristics of the Nanjing local strains of HHV-7,fourstrains of herpesvirus were isolated from saliva specimens of one healthy individual and three childrensuffering from a kidney disease in Nanjing.The viruses were identified by transmission electron microscopy(EM),indirect immunofluorescence assay(IFA) with a specific monoclonal antibody;nested polymerase chainreaction,restriction mapping and DNA sequencing.The virus-infected cells showed the typical cytophathiceffect(CPE) under microscopy and could be detected by IFA with the human herpesvirus-7(HHV-7) specificantibody.Under EM,herpesvirus-like and virions capsids could be found in their cytoplasm or nucleoplasm.HHV-7 DNA fragments amplified from infected cells by nested PCR were confirmed by restriction mappingand DNA sequencing.Similarly to DC strain,an known HHV-7 strain used in the present study as the positivecontrol,the virus could be inactivated by ultraviolet irradiation for 10 min,heated at 45℃ for 30 min,pH<5or>9 at 4℃ for 2 h and ether or chloroform for 10 h.The virus induced the production of TNF-α,IL-10 andIL-12p70 while inhibited IFN-γ secretion,increased the percentage of CD2~+ cells while decreased that of CD4~+or CD45RA~+ cells.The results indicate that the viruses isolated in Nanjing are HHV-7,which has similarbiological characteristic to the known HHV-7 strain,DC.Infection with HHV-7 in vitro could affect immunefunction of lymphocytes by disturbing cytokine production and CD antigen expression.Cellular & MolecularImmunology.2004;1(5):367-372.     

大学生自我表露及其与主观幸福感的关系

目的:探讨大学生自我表露、主观幸福感现况及二者的关系.方法:根据浙江大学164名学生的问卷调查结果对Jourard SM自我表露问卷进行修订,用修订的自我表露问卷和幸福感指数量表对浙江大学的356名学生进行问卷调查.结果:不同信息类别的自我表露评分差异显著(F=50.24,P＜0.001),其中品味和兴趣(1.85±0.41)、学习(工作)(1.87±0.43)的自我表露评分均高于态度和看法(1.70±0.32)(t=7.870、8.256)、金钱(1.60±0.41,t=10.517、11.429)、个性(1.70±0.39)、身体(1.71±0.42)(P均＜0.001).目标人的主效应显著(有男/女朋友者、无男/女朋友者的自我表露问卷评分差异均有显著性,F=21.14、13.88,P＜0.001),信息类别和目标人的交互作用显著(F=76.15,P＜0.001).在金钱、身体、学习或工作方面大学生向父母表露多于向男、女性朋友(如金钱上:母亲1.81±0.59、父亲1.73±0.58,男朋友1.42±0.44,女朋友1.40±0.44;F=116.84,P=0.000).女性的表露程度高于男性(1.79±0.31/1.71±0.32,t=2.03,P=0.044).自我表露与主观幸福感呈正相关(r=0.16,P=0.003).分层回归显示自我表露对预测主观幸福感作出了新的贡献(△R2=0.021,P=0.006).结论:信息类别、目标人、被试性别会影响自我表露的程度,提升表露水平有助于改善主观幸福感.     

重症休克大鼠细动脉平滑肌膜电位变化在血管反应性降低中的作用

目的：研究查明重症失血性休克血管反应性降低的机制。方法：测定大鼠脊斜肌细动脉对去甲肾上腺素（ＮＥ）的反应性，在反应性下降时用微电极记录离体主动脉及细动脉条平滑肌静息膜电位；用激光共聚焦显微镜动态测定单个细动脉平滑肌细胞膜电位变化；观察ＡＴＰ敏感钾通道（ＫＡＴＰ）阻滞剂优降糖对细动脉平滑肌细胞膜电位和血管反应性的作用。结果：在失血性休克代偿期，细动脉平滑肌膜电位负值减小，血管对ＮＥ反应性增高；失血性休克失代偿期，细动脉对ＮＥ反应性显著下降，细动脉平滑肌细胞静息膜电位负值增大，呈超极化改变，优降糖能部分逆转休克时平滑肌细胞的超极化状态，同时带来血管反应性的恢复。结论：休克时细动脉平滑肌反应性与膜电位密切相关，ＫＡＴＰ通道开放引起血管平滑肌细胞膜超极化是重症失血性休克后期血管反应性低下的原因之一。     

大鼠背根神经节内kv2.1 mRNA和kv4.2 mRNA的表达

目的　了解kv2 1和kv4 2型钾离子通道在背根神经节的表达。方法　应用原位杂交方法观察背根神经节内细胞膜钾离子kv2 1和kv4 2型通道的mRNA阳性神经结构的表达。结果　腰 3～ 5背根神经节内均有kv2 1和kv4 2mRNA阳性神经元的表达 ,kv4 2mRNA阳性神经元的数量和总面积明显多于kv2 1阳性神经元。结论　kv2 1和kv4 2型钾离子通道可能参与了背根神经节对外周传入信息的传递和调制。     

胎盘部位过度反应及胎盘部位结节的临床病理分析

目的探讨胎盘部位过度反应及胎盘部位结节的临床病理学特征以及免疫组织化学染色在鉴别诊断中的意义。方法对15例胎盘部位过度反应及4例胎盘部位结节的临床及病理表现进行回顾性研究,并应用人绒毛膜促性腺激素(hCG)、人胎盘催乳素(hPL)、细胞角蛋白(CK)18、胎盘碱性磷酸酶(PLAP)、α-抑制素(inhibin),进行免疫组织化学染色。结果15例胎盘部位过度反应患者的年龄为25～40岁(平均31．5岁),4例胎盘部位结节患者年龄为26～39岁(平均34．3岁)。15例胎盘部位过度反应的组织学特征为：在子宫内膜、子宫肌层及螺旋动脉中有索条状及片状种植部位中间滋养细胞浸润,子宫内膜及肌层的结构没有破坏。4例胎盘部位结节在子宫内膜组织及变性坏死的绒毛间有多个以致密的嗜酸性玻璃样物质为背景的结节性病变,结节内为绒毛膜型中间滋养细胞。15例胎盘部位过度反应对hPL及CK18均呈阳性反应;4例胎盘部位结节均对CK18、α-抑制素及PLAP均呈阳性反应。所有15例胎盘部位过度反应Ki-67增生指数均≤5％。4例胎盘部位结节Ki-67增生指数均为0。结论胎盘部位过度反应及胎盘部位结节的临床及病理形态学特征不同于滋养细胞肿瘤。免疫组织化学染色对鉴别诊断有帮助。