A spontaneous increase of salt intake and changes of colonic temperature in mice exposed to cold

The effect of repeated short-term exposure to cold on spontaneous salt (NaCl) intake and colonic temperature was investigated in two experiments on mice. In Experiment 1, half of a group of test animals were exposed to cold (7-9 degrees C; 6h/day; 4 days), and half of both the exposed and non-exposed animals were allowed to choose between drinking water with (0.9%) and without (0%) NaCl. Food and fluids were provided all day long during the experiment. Mice provided with NaCl solution showed increased salt intake with cold exposure. Colonic temperature of mice was measured twice a day at the beginning and the end of cold exposure. Different changes in colonic temperature among the groups were observed at the end of cold exposure. In mice exposed to cold and provided with NaCl solution, colonic temperatures stayed unchanged, whereas in those without NaCl solution colonic temperatures decreased significantly after cold exposure. In Experiment 2, all the mice were exposed to cold as in Experiment 1. Four patterns of feeding of food and fluids were applied to groups of eight mice each: removal of food and fluids during cold; removal during 9 h before cold and during cold; removal during cold and during 9 h after cold, and feeding all day long. Half the mice in each feeding pattern were allowed to choose NaCl fluids (0% & 0.9%). Colonic temperature was measured as in Experiment 1. In all the feeding patterns, colonic temperature was significantly lower in the mice without NaCl solution than those with NaCl solution. Among four feeding patterns in mice with NaCl solution, colonic temperature was significantly higher in the case of feeding all day long than in the other three patterns.     

肝阳上亢证病人红细胞内核苷酸水平

用高效液相色谱法测定肝阳上亢、阴虚阳亢二证型病人及健康人的红细胞内核苷酸水平。结果表明,肝阳上亢组 ATP,ADP,NADP 含量高于健康人组及阴虚阳亢组,AMP,GTP,NAD含量与健康人组差别无显著性。     

腰椎滑脱29例手术分析

目的 探讨腰椎滑脱的手术治疗方式。方法 对29例腰椎滑脱患者应用RF-Ⅱ或SRS椎弓根内固定系统复位，神经根管松解，10例行横突间条状植骨、11例行自体髂骨块椎体间植骨、8例置入椎间融合器。观察其复位、融合情况。结果 术后患者腰腿痛症状均有改善，滑脱椎体得到了完全复位，术后6个月均达到骨性融合。1例行横突间植骨患者有内固定物松动、退钉现象。结论 RF-Ⅱ、SRS复位效果肯定，椎体间自体髂骨块植入、椎间融合器的使用更符合生物力学要求。     

河南贲门癌高发区贲门癌旁肠上皮化生组织的组织化学特征

目的 :探讨贲门癌癌旁肠上皮化生的组织化学特征。方法 :采用艾显蓝 雪夫 (AB PAS)和高铁二胺 艾显兰 (HID AB)粘液组织化学染色法研究了 49例贲门癌癌旁肠上皮化生的组织化学特点 ,并与 93例胃癌癌旁肠上皮化生做对照。结果 :贲门癌癌旁肠上皮化生的检出率 ( 4 4 9% )明显低于胃癌癌旁的肠上皮化生检出率 ( 80 64 % ) (P <0 .0 1) ;贲门癌癌旁不完全型小肠化生 ( 3 1.82 % )和不完全型大肠化生 ( 63 .64 % )所占肠化的比例均明显高于胃癌癌旁不完全型小肠化生 ( 5 .3 3 % )和大肠化生 ( 2 1.3 3 % )所占肠化的比例 (P <0 .0 1)。结论 :贲门癌旁上皮不完全型肠上皮化生检出率明显高于胃癌癌旁上皮提示其可能是一种重要的贲门癌癌前病变     

快速高效提取白色念珠菌DNA

目的建立两种简便的白色念珠菌ＤＮＡ提取方法，即纯ＤＮＡ提取法和快速ＤＮＡ提取法。方法以原生质体提取法为基础进行改良，两种方法均用Ｌｙｔｉｃａｓｅ破壁。结果与结论纯ＮＤＡ提取法提取ＤＮＡ耗时６～７ｈ，产量较高，每１０~（１０）个菌细胞可提取４μｇＤＮＡ，且纯度高，ＯＤ_（２６０）ＯＤ_（２８０）＞１．７，对核酸限制性内切酶敏感，适用于各种分子生物学方面的研究。快速ＤＮＡ提取法全过程仅需３～４ｈ，每１０~（１０）个菌细胞提取１μｇＤＮＡ，ＯＤ_（２６０）：ＯＤ_（２８０）＝１．４，不能进行核酸限制性内切酶酶切，但可用于作ＰＣＲ扩增。     

不孕夫妇精子－宫颈粘液体外穿透试验与其他不孕因素的关系研究

应用玻片法精子－宫颈粘液穿透试验检测３５０例不孕夫妇。结果显示，不孕夫妇穿透试验评分差和阴性者占６０％。３６例为各项检测正常，仅穿透试验评分差，占１０％。穿透试验评分优良的比率与精子活率、向前直线运动精子的比率以及女性血清抗精子抗体阳性的比率有关。     

SARS疑似病例早期临床特征分析

目的 :本研究对我院发热门诊和隔离病房SARS疑似病例行临床特征的分析 ,以探讨其早期诊断、治疗、病程变化及临床预后。方法 :对 5 8例SARS疑似病例早期诊断依据、跟踪指定医院中临床观察结果进行回顾分析。按卫生部非典型肺炎临床诊断标准 (试行 )和WHO诊断标准 ,并将确诊或疑似病例分为轻、中、重三型。 12例发热患者因普通胸部X线片不能确切判断 ,并行肺部CT检查确定炎症病变。治疗根据病情判断分层次给予抗病毒、抗菌、免疫增强剂、甲泼尼龙、中药方剂、无创正压通气等治疗。结果 :5 8例疑似病例中最后确诊SARS 4 9例 ,确诊率 84 .5 %。轻型 :8例 (16 .3% ) ;中度型 :34例 (6 9.3% ) ;重型 :7例 (14 .2 % ) ,其中 2例 (4 % )转入指定专门医院后死亡。轻型患者入院后多无再发热 ,平均住院时间 8.6d ;中度型 :可反复发热数日 ,经抗病毒、抗菌药治疗 ,并多数给甲泼尼龙治疗 (92 % )出院时胸片暗影基本吸收 ,平均住院时间 2 1.3d ;重型SARS患者以高热、乏力为首发症状 ,胸片均可见双肺多处病变 ,进展迅速。病情恶化期大剂量应用甲泼尼龙 ,应用无创正压通气 ,平均住院时间≥ 30d。重型SARS患者体温动态观察多持续 2～ 3d ,用解热或激素均可退热 ,应用激素期间仍于 6～ 8d出现第二次发热 ,多数患者 12～ 1