大豆皂甙和神经肽Y在下丘脑后核引起的心血管效应中的关系

微量注射大豆皂甙（Ｔｓ）到下丘脑后核（ＰＨＮ）能引起平均动脉压（ＭＡＰ）升高，心率加快，其效应与向ＰＨＮ内注入神经肽Ｙ（ＮＰＹ）相似。分别注入不能引起血压、心率发生明显变化的微量Ｔｓ、ＮＰＹ，如果合并注入ＰＨＮ则可引起血压、心率的显著变化，表明二者互有加强作用。Ａｔｒｏｐｉｎｅ可以对抗Ｔｓ、ＮＰＹ或Ｔｓ＋ＮＰＹ同时注入ＰＨＮ的升压效应，提示ＮＰＹ是通过激活脑内Ｍ受体而实现升压效应的，而Ｔｓ的心血管效应又可能是经过脑内ＮＰＹ介导和加强的。     

Effect of eucalyptus globulus oil on activation of nuclear factor-kappaB in THP-1 cells]

OBJECTIVE: To study the effect of eucalyptus globulus oil on the activity of nuclear factor-kappaB(NF-kappaB) in THP-1 cell line. METHODS: THP-1 cells were cultured with or without eucalyptus globulus oil at different concentrations (1, 10, 100 mg x L(-1), 30 min) before being stimulated with lipopolysaccharide (LPS, 1 mg x L(-1), 30 min). The location of NF-kappaB p65 subunit (NF-kappaB/p65) in THP-1 cells was detected by indirect immunofluorescence and laser scanning confocal microscope. The expression of NF-kappaB/p65 in nuclei was measured by Western-blot analysis. RESULT: The FITC-label NF-kappaB/p65 was mainly located in the nuclei after THP-1 cells were stimulated with LPS. Whereas, no fluorescence were seen in the nuclei of cells pretreated with eucalyptus globulus oil. This effect on NF-kappaB/p65 nuclear translocation was in a concentration dependent manner. CONCLUSION: Eucalyptus globulus oil inhibits the nuclear translocation of NF-kappaB induced by LPS in THP-1 cells.     

舰船员慢性荨麻疹与马拉色菌相关性研究

OBJECTIVE: To investigate the association of Malassezia furfur with chronic urticaria in the crew members of ships. METHODS: A comparative mycological study of 126 crew members of ships with chronic urticaria and 45 normal control subjects was carried out. The 82 urticaria patients identified as positive for Malassezia furfur were divided into groups A and B to receive treatment with antihistaminics (group A) and antihistaminics combined with 2% ketoconazole shampoo(group A). RESULTS: The carrier rates of Malassezia furfur were significantly higher in urticaria patients than in the normal control subjects (P<0.01), but in view of the case ratios of the final cure or improvement, no significant difference was observed between the two groups by the end of the treatment courses (P>0.05). But 6 to 8 weeks from the end of the treatment course, better therapeutic effect was noted in group B (P<0.01), with higher rate of negative Malassezia furfur findings (P<0.01). CONCLUSION: Malassezia furfur may play an important role in the prevalence of chronic urticaria among the crew members, and anti-fungal treatment may produce better long-term therapeutic effect.     

准分子激光角膜原位磨镶术治疗近视合并中度以上散光疗效观察

目的 :评价小光斑飞点扫描准分子激光系统应用于准分子激光原位角膜磨镶术 (LASIK)治疗近视合并中度以上散光的疗效。　方法 :对 96例患者 14 4只眼按术前屈光度分成三组 ,Ⅰ组 75只眼 ,近视 0～ - 6D ,散光 - 2～ - 4DC ;Ⅱ组 5 7只眼 ,近视 - 6 .2 5～ 10 .0 0D ,散光 - 2～ - 4DC。Ⅲ组 12只眼 ,近视 - 10 .2 5D以上 ,散光 - 2～- 4DC ;术后随访半年以上 ,并将结果进行比较分析。　结果 :术后 1个月裸眼视力达到 0 .6以上者 ,Ⅰ组为5 .3% ,Ⅱ组为 15 .8% ,Ⅲ组为 6 6 .7% ;术后 1个月裸眼视力达到 1.0以上者 ,Ⅰ组为 94 .7% ,Ⅱ组为 84 .2 % ,Ⅲ组为 33.3%。术后半年裸眼视力达到 0 .6以上者 ,Ⅰ组为 4 % ,Ⅱ组为 14 % ,Ⅲ组为 75 % ;术后半年裸眼视力达到 1.0以上者 ,Ⅰ组为 96 % ,Ⅱ组为 86 % ,Ⅲ组为 2 5 %。术后屈光度在± 1.0D以内者 ,Ⅰ组中为 8% ,Ⅱ组为 14 % ,Ⅲ组为 5 8.3%。术后屈光度在± 0 .5 0D以内者 ,Ⅰ组为 92 % ,Ⅱ组为 86 % ,Ⅲ组为 4 1.7%。术后散光度在± 1.0DC以内者 ,Ⅰ组为 8% ,Ⅱ组为 10 .5 % ,Ⅲ组为 5 0 %。术后散光度在± 0 .5 0DC以内者 ,Ⅰ组为 92 % ,Ⅱ组为 89.5 % ,Ⅲ组为 5 0 %。平均实际切削区直径为 (8.0 2± 0 .0 6 9)mm。　结论 :使用小光斑飞点显像LASIK治疗低     

巨噬细胞炎性蛋白4的突变和原核表达

目的 探索如何抑制嗜酸细胞的趋化作用，选择β-趋化因子巨噬细胞炎性蛋白4（MIP4）的突变性（Met-MIP4)作为趋化因子受体3的拮抗剂，将Met-MIP4基因在原核细胞中进行表达。方法 设计MIP4基因的PCR引物并进行氨基酸突变，将MIP4N末端的丙氨酸突变为蛋氨酸，以正常人肺酸突变，将MIP4N末端的丙氨酸突变为蛋氨酸。以正常人肺cDNA文库为模板，PCR方法获取Met-MIP4基因，克隆入载体pUC19，测序验证序列已得到突变，将正确的基因插入到GST融合表达载体pGEX-4T中，以IPTG诱导表达。结果 PCR产物为220bp左右的片段，连接入pUC19质粒后测序验证获得正确突变，构建的pGEX-4T融合表达载体在大肠杆菌中表达，经SDS-PAGE凝胶电泳显示有大小约34kU的新生融合蛋白表达。结论 成功突变并克隆了β-趋化因子MIP4基因，SDS-PAGE表明，与GST融合的Met-MIP4突变体已得到表达，为进一步研究其生物学活性奠定了基础。     

胸腺切除后血清乙酰胆碱受体抗体改变及与疗效关系

随访２３例因重症肌无力行胸腺切除术的病人。运用灵敏度高、特异性强的ＢＡＳ—ＥＬＩＳＡ法检测手术前、后血清乙酰胆碱受体抗体（ＡｃｈＲ抗体）水平，所得结果均进行统计学分析。２３例病人胸腺切除后，１８例临床症状改善，有效率７８％。术后１４例（６１％）病人血清ＡｃｈＲ抗体水平下降，统计结果表明，手术前后血清ＡｃｈＲ抗体水平差异有显著性（Ｐ＜０．０５），但与症状改善无关（Ｐ＞０．０５）。手术前后血清ＡｃｈＲ抗体水平不影响疗效（Ｐ＞０．０５）。     

链置换式扩增检测羊水中巨细胞病毒DNA

目的：介绍一种简便快速准确检测羊水中ＣＭＶ－ＤＮＡ的改良ＰＣＲ－链置换式扩增用于诊断胎儿先天感染ＣＭＶ。方法，将组成套式ＰＣＲ的外内两对引物按照一定比例（外：内＝１：５０－１００）加在同一试管中一次扩增羊水和胎儿组织中ＣＭＶ－ＤＮＡ。结果：９０例异常孕产史的孕妇羊水检测ＣＭＶ－ＤＮＡ，阳性率为３８．９％（３５／９０），其中合并染色数目异常２例（４７，ＸＹＹ和４７，ＸＸ，＋２１）（已引产）核型及染色     

A method of cephalometric analysis]

In this paper, 220 children ranging from age 11 to 15 were selected. Each of them received a cephalometric roentgenographic examination. 153 cephalometric items from 13 types of methods which had been widely in use were studied through statistic analysis. By means of "t" test, several items which expressed little difference between the normal occlusion and the malocclusion were eliminated. By using of the cluster analysis we selected one item to represent the items which were with similar effect. Finally, 17 cephalometric items were pick out to provide references for clinical work.     

TREATMENT OF HYALINEMEMBRANE DISEASE BYHUMAN AMNIOTIC FLUIDSURFACTANT

A case of hyaline membrane disease was treated successfully with pulmonary surfactant (PS) isolated from human amniotic fluid. Dosage was 150 mg phospholipid/kg. The exogenous surfactant was instilled into the airway via a tracheal cannula. Clinical symptoms, PO2 and FiO2 improved evidently 24 hours after administration. L/S ratio and phosphatidylglycerol recovered gradually in aspirates. Lung X-ray film manifested "white lung" before instillation of surfactant and showed a striking improvement 3 days after treatment. The duration of mechanical ventilation was 6 days. During the period of recovery complications of patent ductus arteriosus and bacterial pneumonia developed. However, the patient recovered completely and was discharged 32 days after admission.