非意愿妊娠妇女对紧急避孕知识认知程度的调查

为了解非意愿妊娠妇女对紧急避孕知识的认知程度 ,以预防意外妊娠 ,降低人工流产率 ,保护妇女身心健康 ,采用自行设计的问卷 ,随机对 2 2 0例非意愿妊娠妇女进行了调查。结果 64.5 5 %的妇女完全不了解紧急避孕方法 ;在表示了解的妇女中 ,仅 3 8.4 6%所叙述的方法是完全正确的 ;使用过紧急避孕方法者占 19.5 5 % ;79.5 5 %的妇女迫切需要给予指导。在对紧急避孕知识的了解程度、使用情况及指导需要上 ,妇女的婚姻状况不同 ,差异均无显著性意义 ( P>0 .0 5 ) ;而文化程度不同则差异有显著性或极显著性意义 ( P<0 .0 5或 P<0 .0 1)。提示迫切需要加强紧急避孕知识的宣传教育工作 ,以降低非意愿妊娠的发生 ,文化程度低者应成为教育的重点对象     

心理治疗中的相通心理致病机制

分析了4大主要治疗流派——古典精神分析、行为疗法、当事人中心疗法、理性情绪疗法有关致病机制的论述,总结出以下相通的心理致病机制:当事人对自己情感经验的过分压抑防御,导致人格的僵化、固执,使自我的疆界萎缩;当事人自主力量缺乏,无力控制自己的局面,造成了人格局部的昏乱、冲突;人格局部的失调又导致人格整体的失调或人格的整合不良。     

Hepatotoxicity of iodine-131 ablation for post-surgical differentiated thyroid cancer patients with hepatitis B virus infection

ObjectiveRadioiodine (Iodine-131, ¹³¹I) ablation is a standard treatment for differentiated thyroid cancer (DTC) after thyroidectomy. Hepatotoxicity is a rare side effect of ¹³¹I, and little information is available on the hepatotoxicity of ¹³¹I ablation for post-surgical DTC patients with hepatitis B virus (HBV) infection.MethodsWe performed a retrospective study of 94 post-surgical DTC patients between November 2012 and August 2015 in our hospital. All the patients had been screened for HBV infection and divided into HBV group and non-HBV group. Clinical data were compared between the two groups.Results14 patients with HBV infection and 80 patients without HBV infection were analyzed. The baseline characteristics of the two groups had no statistical differences. Incidence of hepatotoxicity was higher in HBV group than in non-HBV group and HBV infection was confirmed as a risk factor of hepatotoxicity by univariate and multivariate regression analysis.ConclusionPost-surgical DTC patients with HBV infection were prone to hepatotoxicity by ¹³¹I ablation treatment. Physicians should pay more attention to the liver function of patients at risk.     

替诺福韦治疗不同HBV基因型慢性乙型肝炎患者疗效研究*

目的 探讨替诺福韦治疗不同HBV基因型感染的慢性乙型肝炎(CHB)患者的疗效和血清金属硫蛋白(MT)、白细胞介素29(IL-29)和外周血淋巴细胞程序性死亡受体-1(PD-1)表达的变化。方法 2017年1月～2020年11月我院诊治的CHB患者119例,均接受替诺福韦治疗。采用ELISA法检测血清MT和IL-29水平,使用流式细胞仪检测外周血T 淋巴细胞PD-1表达水平。结果 在本组119例CHB患者中,检出HBV B基因型31例(26.1%),C基因型77例(64.7%),B/C 混合基因型11例(9.2%);基线资料比较,B基因型CHB患者血清ALT和AST水平分别为(154.1±46.7)U/L和(83.3±26.8)U/L,显著高于C基因型患者【分别为(135.8±40.3)U/L和(68.5±20.6)U/L,P＜0.05】或B/C混合基因型患者【分别为(138.9±50.2)U/L和(71.6±23.9)U/L,P＜0.05】,而血清HBV DNA水平为(6.7±1.1)lg copies/ml,显著低于C基因型感染患者【(7.8±1.4)lg copies/ml,P＜0.05】或B/C混合型感染者【(7.4±1.0)lg copies/ml,P＜0.05】;在治疗24 w和48 w末,三组血清ALT复常率无显著性差异(P>0.05),而B基因型和C基因型感染患者血清HBV DNA阴转率分别为96.8%和96.8%,和88.3%和93.5%,均显著高于B/C混合型感染患者(分别为63.6%和81.8%,P<0.05);在治疗48 w,B基因型CHB患者血清MT水平显著高于C基因型或B/C基因型(P＜0.05),血清IL-29水平显著高于C基因型(P＜0.05),而外周血CD4⁺和CD8⁺T淋巴细胞表面PD-1表达水平显著低于C基因型或B/C混合基因型患者(P＜0.05)。结论 不同HBV基因型感染的CHB患者可能对替诺福韦治疗的疗效存在差异,深入了解这些差异可能对研究不同基因型病毒感染患者临床转归有帮助。     

孟鲁司特钠用于上呼吸道感染后出现咳嗽症状患儿的临床疗效分析

目的探讨孟鲁司特钠用于上呼吸道感染后出现咳嗽症状患儿的临床疗效。方法选择该院2012年9月—2014年9月上呼吸道感染后咳嗽不止的患儿186例,采用隐匿数字随机法分为两组,对照组93例给予镇咳祛痰药物,观察组在上述治疗的同时加服孟鲁司特钠,动态观察症状改善情况,并评价疗效。结果治疗后6 d、9 d两组咳嗽评分明显低于治疗前(P<0.05),观察组咳嗽评分降低幅度明显大于对照组(P<0.05)。观察组总有效率92.47%,明显高于对照组(P<0.05)。观察组平均咳嗽缓解时间、平均治疗时间短于对照组,9 d症状改善率高于对照组,组间比较,差异有统计学意义(P<0.05)。结论镇咳祛痰药物治疗的同时辅以孟鲁司特钠治疗小儿上呼吸道感染后咳嗽,可明显缩短咳嗽时间和疗程,症状改善明显,有临床应用价值。     

Design,Synthesis and Characterization of HIV-1 CA-Targeting Small Molecules: Conformational Restriction of PF74

Small molecules targeting the PF74 binding site of the HIV-1 capsid protein (CA) confer potent and mechanistically unique antiviral activities. Structural modifications of PF74 could further the understanding of ligand binding modes, diversify ligand chemical classes, and allow identification of new variants with balanced antiviral activity and metabolic stability. In the current work, we designed and synthesized three series of PF74-like analogs featuring conformational constraints at the aniline terminus or the phenylalanine carboxamide moiety, and characterized them using a biophysical thermal shift assay (TSA), cell-based antiviral and cytotoxicity assays, and in vitro metabolic stability assays in human and mouse liver microsomes. These studies showed that the two series with the phenylalanine carboxamide moiety replaced by a pyridine or imidazole ring can provide viable hits. Subsequent SAR identified an improved analog 15 which effectively inhibited HIV-1 (EC₅₀ = 0.31 μM), strongly stabilized CA hexamer (ΔTm = 8.7 °C), and exhibited substantially enhanced metabolic stability (t_1/2 = 27 min for 15 vs. 0.7 min for PF74). Metabolic profiles from the microsomal stability assay also indicate that blocking the C5 position of the indole ring could lead to increased resistance to oxidative metabolism.     

NuRD subunit MTA1 interacts with the DNA non-homologous end joining Ku complex in cancer cells

Metastasis-associated antigen 1 (MTA1) is a chromatin modifier mediating DNA modification and gene expression. Ku70/Ku80 complex has been reported to be essential in DNA damage response. In an effort to explore the MTA1 interactome, we captured the Ku70/Ku80 complex with two specific MTA1 antibodies in a colon cancer cell line. We first validated the in vitro interaction between MTA1 and the Ku complex by co-immunoprecipitation (co-IP) analyses in cell lysate, showing that the interaction occurred mainly at the nucleus, but also existed in the cytoplasm at a lower level. We further visualized and confirmed their in vivo interaction using proximity ligation assay (PLA), which, in line with the in vitro analysis, also demonstrated a vast majority of interaction plots in the nucleus and a small number in the cytoplasm. We previously demonstrated that MTA1 distributed dynamically and periodically during the cell cycle. Here, through fluorescent colocalization, we found that MTA1 and Ku proteins colocalized well in the nucleus at interphase and moved synchronously from prophase to anaphase. Interestingly, at the time of telophase, when MTA1 was reported to re-enter the nucleus, they were separated and moved non-synchronously. Moreover, using in situ PLA, we visualized that the interaction occurred at both interphase and mitosis. At interphase, they interacted mainly in the nucleus, but during mitosis, they interact at the periphery of chromosomes. We also showed that MTA1 correlated well with Ku in both the cancerous and normal tissues, and that they cooperated in UV-induced DNA damage response. Collectively, our data uncover a specific interaction between MTA1 and Ku complex at both the nucleus and cytoplasm, and across the whole cell cycle. We therefore propose a potential functional crosstalk between NuRD and Ku complexes, the two most fundamental function units in cells, via physical interaction.

MTA1 interacts with Ku complex mainly in the nucleus at interphase and surrounding the chromosome during mitosis.     

Aqueous-based electrospun P(NIPAAm-co-AAc)/RSF medicated fibrous mats for dual temperature- and pH-responsive drug controlled release

This paper presents a green method for fabricating dual temperature- and pH-responsive electrospun fibrous mats from an aqueous-based blend poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAAm-co-AAc)) and regenerated silk fibroin (RSF) by employing electrospinning technique. P(NIPAAm-co-AAc) was synthesized by free radical solution polymerization and its low critical solution temperature (LCST) was in the physiological range (38.8 °C). The P(NIPAAm-co-AAc)/RSF fibers were prepared by electrospinning technology in the presence of the crosslinking agents (EDC·HCl and NHS) with water as solvent. After in situ crosslinking and water-annealing process, the water-stable composite fibrous mats were obtained. Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) were used to analyze the crosslinking process. Temperature and pH dual stimuli-responsive swelling-shrinking behavior of the fibrous mats were observed when the temperature was below and above the LCST of the copolymer at different pHs. In addition, rhodamine B-loaded the fibrous mats also showed dual temperature and pH controlled release behavior, demonstrating the potential use of the fibrous mats for “smart” controlled drug delivery applications.

This paper presents a green friendly method for preparing dual temperature- and pH-responsive electrospun P(NIPAAm-co-AAc)/RSF fibrous mats for drug release.     

Layer-by-layer decoration of MOFs on electrospun nanofibers

The design and fabrication of novel organic–inorganic nanocomposite membranes using metal–organic frameworks as building blocks have attracted numerous scientists. Here, HKUST-1 particles were decorated on crosslinked polymer nanofibers through a layer-by-layer method. The immersion sequence, the crosslinking and the number of the deposition cycles have a significant impact on the formation of the HKUST-1 decorated nanofibrous membranes. Moreover, it has been shown that such a membrane could be applied as a catalyst for visual detection of hydrogen peroxide.

A layer-by-layer method was introduced to fabricate MOF-decorated electrospun nanofibers, which could be used for visual detection of hydrogen peroxide.     

整合素α5β1和细胞外信号调节激酶信号传导通路在非小细胞肺癌中的作用及其相关性研究

研究整合素α5β1 及磷酸化ERK1/2 （p-ERK1/2） 在非小细胞肺癌（NSCLC）组织中的表达及整合素α5β1 和ERK1/2 信号通路在人肺癌A549 细胞生长和迁移中的作用。方法:采用免疫组织化学法和免疫印迹（Western blot）法检测41 例NSCLC 组织和15例正常肺组织中整合素α5β1 及p-ERK1/2 的表达并分析两者的相关性,体外培养肺癌A549 细胞,以Anti-α5β1 或ERK 激酶抑制剂PD98059 作为工具药物,采用四甲基偶氮唑盐比色 （MTT） 法和 Annexin-V FITC PI 双染色法检测A549 增殖和凋亡,采用Western blot 检测A549 中MMP-9 蛋白水平。采用逆转录-聚合酶链反应（RT-PCR）和Western blot 检测Anti-α5β1 处理后A549 中ERK1/ 2 蛋白和mRNA 表达水平的变化。结果:人NSCLC 组织中整合素α5β1 及p-ERK1/2 阳性表达率（分别为58.53%,65.52%）明显高于正常肺组织（分别为26.67%, 20.00%）（P<0.01）。整合素α5β1与p-ERK1/2 表达均与病理分级（分别为P<0.001,P=0.030）、淋巴结转移（分别为P=0.014,P<0.001）、临床分期（分别为P=0.027,P=0.038）相关。整合素α5β1 与p-ERK1/2表达具有相关性（r=0.375, P<0.05）。经Anti-α5β1 或PD98059 处理后,A549 细胞增殖效应和早期凋亡细胞百分率增加,MMP-9 的表达均明显减弱。经Anti-α5β1 处理后A549 细胞中ERK 的mRNA 和蛋白表达受到抑制,ERK1/2 的磷酸化水平显著减少。结论: 整合素α5β1 及p-ERK1/2 在人NSCLC 组织中高表达,Anti-α5β1 在下调了细胞内ERK 的mRNA 和蛋白磷酸化的同时,可以明显抑制A549 细胞的增殖和MMP-9 蛋白表达,表明整合素α5β1 可能介导ERK1/2 信号转导通路参与了非小细胞肺癌中的异常增殖和迁移的调控。